TSKS_HUMAN
ID TSKS_HUMAN Reviewed; 592 AA.
AC Q9UJT2; Q8WXJ0;
DT 01-MAR-2004, integrated into UniProtKB/Swiss-Prot.
DT 01-MAR-2001, sequence version 3.
DT 03-AUG-2022, entry version 139.
DE RecName: Full=Testis-specific serine kinase substrate;
DE Short=Testis-specific kinase substrate;
DE AltName: Full=STK22 substrate 1;
GN Name=TSKS; Synonyms=STK22S1, TSKS1;
OS Homo sapiens (Human).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae;
OC Homo.
OX NCBI_TaxID=9606;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND TISSUE SPECIFICITY.
RX PubMed=11444856; DOI=10.1006/bbrc.2001.5165;
RA Scorilas A., Yousef G.M., Jung K., Rajpert-De Meyts E., Carsten S.,
RA Diamandis E.P.;
RT "Identification and characterization of a novel human testis-specific
RT kinase substrate gene which is downregulated in testicular tumors.";
RL Biochem. Biophys. Res. Commun. 285:400-408(2001).
RN [2]
RP NUCLEOTIDE SEQUENCE [MRNA], PHOSPHORYLATION, INTERACTION WITH TSSK2, TISSUE
RP SPECIFICITY, AND VARIANT GLY-382.
RX PubMed=15044604; DOI=10.1093/molehr/gah052;
RA Hao Z., Jha K.N., Kim Y.H., Vemuganti S., Westbrook V.A., Chertihin O.,
RA Markgraf K., Flickinger C.J., Coppola M., Herr J.C., Visconti P.E.;
RT "Expression analysis of the human testis-specific serine/threonine kinase
RT (TSSK) homologues. A TSSK member is present in the equatorial segment of
RT human sperm.";
RL Mol. Hum. Reprod. 10:433-444(2004).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC TISSUE=Brain;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [4]
RP SUBCELLULAR LOCATION.
RX PubMed=18495105; DOI=10.1016/j.ydbio.2008.03.043;
RA Xu B., Hao Z., Jha K.N., Zhang Z., Urekar C., Digilio L., Pulido S.,
RA Strauss J.F. III, Flickinger C.J., Herr J.C.;
RT "TSKS concentrates in spermatid centrioles during flagellogenesis.";
RL Dev. Biol. 319:201-210(2008).
RN [5]
RP VARIANT [LARGE SCALE ANALYSIS] ARG-19.
RX PubMed=16959974; DOI=10.1126/science.1133427;
RA Sjoeblom T., Jones S., Wood L.D., Parsons D.W., Lin J., Barber T.D.,
RA Mandelker D., Leary R.J., Ptak J., Silliman N., Szabo S., Buckhaults P.,
RA Farrell C., Meeh P., Markowitz S.D., Willis J., Dawson D., Willson J.K.V.,
RA Gazdar A.F., Hartigan J., Wu L., Liu C., Parmigiani G., Park B.H.,
RA Bachman K.E., Papadopoulos N., Vogelstein B., Kinzler K.W.,
RA Velculescu V.E.;
RT "The consensus coding sequences of human breast and colorectal cancers.";
RL Science 314:268-274(2006).
CC -!- FUNCTION: May play a role in testicular physiology, most probably in
CC the process of spermatogenesis or spermatid development.
CC -!- INTERACTION:
CC Q9UJT2; Q7Z3Y8: KRT27; NbExp=3; IntAct=EBI-852101, EBI-3044087;
CC Q9UJT2; P06753: TPM3; NbExp=3; IntAct=EBI-852101, EBI-355607;
CC Q9UJT2; Q9BXA7: TSSK1B; NbExp=3; IntAct=EBI-852101, EBI-6423734;
CC Q9UJT2; Q96PF2: TSSK2; NbExp=9; IntAct=EBI-852101, EBI-852089;
CC Q9UJT2-2; Q96PF2: TSSK2; NbExp=2; IntAct=EBI-852113, EBI-852089;
CC -!- SUBCELLULAR LOCATION: Cytoplasm, cytoskeleton, microtubule organizing
CC center, centrosome, centriole {ECO:0000269|PubMed:18495105}.
CC Note=Concentrates in spermatid centrioles during flagellogenesis.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=1;
CC IsoId=Q9UJT2-1; Sequence=Displayed;
CC Name=2;
CC IsoId=Q9UJT2-2; Sequence=VSP_009591, VSP_009592, VSP_009593;
CC -!- TISSUE SPECIFICITY: Highly expressed in testis. Expressed at low levels
CC in prostate, female breast, placenta, ovary and thymus.
CC {ECO:0000269|PubMed:11444856, ECO:0000269|PubMed:15044604}.
CC -!- PTM: Phosphorylated on serine residue(s) by STK22A/TSSK1 and
CC STK22B/TSSK2. {ECO:0000269|PubMed:15044604}.
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; AF200923; AAF12819.3; -; Genomic_DNA.
DR EMBL; AF411384; AAL60464.1; -; mRNA.
DR EMBL; BC058862; AAH58862.1; -; mRNA.
DR CCDS; CCDS12780.1; -. [Q9UJT2-1]
DR PIR; JC7709; JC7709.
DR RefSeq; NP_068379.1; NM_021733.1. [Q9UJT2-1]
DR AlphaFoldDB; Q9UJT2; -.
DR SMR; Q9UJT2; -.
DR BioGRID; 121902; 43.
DR IntAct; Q9UJT2; 32.
DR STRING; 9606.ENSP00000246801; -.
DR iPTMnet; Q9UJT2; -.
DR PhosphoSitePlus; Q9UJT2; -.
DR BioMuta; TSKS; -.
DR DMDM; 44888550; -.
DR EPD; Q9UJT2; -.
DR MassIVE; Q9UJT2; -.
DR PaxDb; Q9UJT2; -.
DR PeptideAtlas; Q9UJT2; -.
DR PRIDE; Q9UJT2; -.
DR ProteomicsDB; 84647; -. [Q9UJT2-1]
DR ProteomicsDB; 84648; -. [Q9UJT2-2]
DR Antibodypedia; 18691; 123 antibodies from 20 providers.
DR DNASU; 60385; -.
DR Ensembl; ENST00000246801.8; ENSP00000246801.2; ENSG00000126467.11. [Q9UJT2-1]
DR GeneID; 60385; -.
DR KEGG; hsa:60385; -.
DR MANE-Select; ENST00000246801.8; ENSP00000246801.2; NM_021733.2; NP_068379.1.
DR UCSC; uc002ppm.4; human. [Q9UJT2-1]
DR CTD; 60385; -.
DR DisGeNET; 60385; -.
DR GeneCards; TSKS; -.
DR HGNC; HGNC:30719; TSKS.
DR HPA; ENSG00000126467; Tissue enriched (testis).
DR MIM; 608253; gene.
DR neXtProt; NX_Q9UJT2; -.
DR OpenTargets; ENSG00000126467; -.
DR PharmGKB; PA164727339; -.
DR VEuPathDB; HostDB:ENSG00000126467; -.
DR eggNOG; ENOG502RT0F; Eukaryota.
DR GeneTree; ENSGT00390000002611; -.
DR HOGENOM; CLU_033722_0_0_1; -.
DR InParanoid; Q9UJT2; -.
DR OMA; DYMHLKM; -.
DR OrthoDB; 732555at2759; -.
DR PhylomeDB; Q9UJT2; -.
DR TreeFam; TF337594; -.
DR PathwayCommons; Q9UJT2; -.
DR SignaLink; Q9UJT2; -.
DR BioGRID-ORCS; 60385; 13 hits in 1088 CRISPR screens.
DR GenomeRNAi; 60385; -.
DR Pharos; Q9UJT2; Tbio.
DR PRO; PR:Q9UJT2; -.
DR Proteomes; UP000005640; Chromosome 19.
DR RNAct; Q9UJT2; protein.
DR Bgee; ENSG00000126467; Expressed in right testis and 109 other tissues.
DR ExpressionAtlas; Q9UJT2; baseline and differential.
DR Genevisible; Q9UJT2; HS.
DR GO; GO:0005814; C:centriole; IDA:UniProtKB.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-KW.
DR GO; GO:0019901; F:protein kinase binding; IPI:UniProtKB.
DR InterPro; IPR028214; TSKS.
DR PANTHER; PTHR14351; PTHR14351; 1.
DR Pfam; PF15358; TSKS; 1.
PE 1: Evidence at protein level;
KW Alternative splicing; Cytoplasm; Cytoskeleton; Phosphoprotein;
KW Reference proteome.
FT CHAIN 1..592
FT /note="Testis-specific serine kinase substrate"
FT /id="PRO_0000065665"
FT REGION 232..308
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 566..592
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 235..264
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 566..585
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOD_RES 217
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:P60531"
FT MOD_RES 288
FT /note="Phosphoserine; by TSSK1 and TSSK2"
FT /evidence="ECO:0000250|UniProtKB:O54887"
FT MOD_RES 316
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:P60531"
FT VAR_SEQ 1..200
FT /note="Missing (in isoform 2)"
FT /evidence="ECO:0000305"
FT /id="VSP_009591"
FT VAR_SEQ 201..221
FT /note="RSVEDAEIKTNVLKQNSALLE -> MGDSCPAVTFRSQKENRAGLQ (in
FT isoform 2)"
FT /evidence="ECO:0000305"
FT /id="VSP_009592"
FT VAR_SEQ 254..259
FT /note="Missing (in isoform 2)"
FT /evidence="ECO:0000305"
FT /id="VSP_009593"
FT VARIANT 19
FT /note="G -> R (in a colorectal cancer sample; somatic
FT mutation; dbSNP:rs149529240)"
FT /evidence="ECO:0000269|PubMed:16959974"
FT /id="VAR_036272"
FT VARIANT 167
FT /note="E -> K (in dbSNP:rs34701020)"
FT /id="VAR_051459"
FT VARIANT 382
FT /note="D -> G (in dbSNP:rs2304202)"
FT /evidence="ECO:0000269|PubMed:15044604"
FT /id="VAR_051460"
FT CONFLICT 272
FT /note="S -> G (in Ref. 2; AAL60464)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 592 AA; 65050 MW; 87B262601EECE121 CRC64;
MASVVVKTIW QSKEIHEAGD TPTGVESCSQ LVPEAPRRVT SRAKGIPKKK KAVSFHGVEP
QMSHQPMHWC LNLKRSSACT NVSLLNLAAM EPTDSTGTDS TVEDLSGQLT LAGPPASPTL
PWDPDDADIT EILSGVNSGL VRAKDSITSL KEKTNRVNQH VQSLQSECSV LSENLERRRQ
EAEELEGYCI QLKENCWKVT RSVEDAEIKT NVLKQNSALL EEKLRYLQQQ LQDETPRRQE
AELQEPEEKQ EPEEKQEPEE KQKPEAGLSW NSLGPAATSQ GCPGPPGSPD KPSRPHGLVP
AGWGMGPRAG EGPYVSEQEL QKLFTGIEEL RREVSSLTAR WHQEEGAVQE ALRLLGGLGG
RVDGFLGQWE RAQREQAQTA RDLQELRGRA DELCTMVERS AVSVASLRSE LEGLGPLKPI
LEEFGRQFQN SRRGPDLSMN LDRSHQGNCA RCASQGSQLS TESLQQLLDR ALTSLVDEVK
QRGLTPACPS CQRLHKKILE LERQALAKHV RAEALSSTLR LAQDEALRAK NLLLTDKMKP
EEKMATLDHL HLKMCSLHDH LSNLPLEGST GTMGGGSSAG TPPKQGGSAP EQ
