ID   TSKS_RAT                Reviewed;         585 AA.
AC   P60531;
DT   01-MAR-2004, integrated into UniProtKB/Swiss-Prot.
DT   01-MAR-2004, sequence version 1.
DT   03-AUG-2022, entry version 95.
DE   RecName: Full=Testis-specific serine kinase substrate;
DE            Short=Testis-specific kinase substrate;
DE   AltName: Full=STK22 substrate 1;
GN   Name=Tsks; Synonyms=Stk22s1;
OS   Rattus norvegicus (Rat).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC   Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC   Murinae; Rattus.
OX   NCBI_TaxID=10116;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA].
RC   STRAIN=Sprague-Dawley;
RA   Zhou G., Zhang Y., Zhang Z.;
RT   "Cloning of three Rattus norvegicus testis specific serine kinases.";
RL   Submitted (JUL-2003) to the EMBL/GenBank/DDBJ databases.
RN   [2]
RP   PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-224; SER-281 AND SER-309, AND
RP   IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RX   PubMed=22673903; DOI=10.1038/ncomms1871;
RA   Lundby A., Secher A., Lage K., Nordsborg N.B., Dmytriyev A., Lundby C.,
RA   Olsen J.V.;
RT   "Quantitative maps of protein phosphorylation sites across 14 different rat
RT   organs and tissues.";
RL   Nat. Commun. 3:876-876(2012).
CC   -!- FUNCTION: May play a role in testicular physiology, most probably in
CC       the process of spermatogenesis or spermatid development.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm, cytoskeleton, microtubule organizing
CC       center, centrosome, centriole {ECO:0000250}. Note=Concentrates in
CC       spermatid centrioles during flagellogenesis. {ECO:0000250}.
CC   -!- PTM: Phosphorylated on serine residue(s) by STK22A/TSSK1 and
CC       STK22B/TSSK2. {ECO:0000250}.
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DR   EMBL; AY346104; AAQ24209.1; -; mRNA.
DR   RefSeq; NP_954519.1; NM_199088.1.
DR   AlphaFoldDB; P60531; -.
DR   SMR; P60531; -.
DR   STRING; 10116.ENSRNOP00000043218; -.
DR   iPTMnet; P60531; -.
DR   PhosphoSitePlus; P60531; -.
DR   PaxDb; P60531; -.
DR   PRIDE; P60531; -.
DR   Ensembl; ENSRNOT00000049584; ENSRNOP00000043218; ENSRNOG00000020443.
DR   GeneID; 292890; -.
DR   KEGG; rno:292890; -.
DR   UCSC; RGD:727784; rat.
DR   CTD; 60385; -.
DR   RGD; 727784; Tsks.
DR   eggNOG; ENOG502RT0F; Eukaryota.
DR   GeneTree; ENSGT00390000002611; -.
DR   HOGENOM; CLU_033722_0_0_1; -.
DR   InParanoid; P60531; -.
DR   OrthoDB; 732555at2759; -.
DR   PhylomeDB; P60531; -.
DR   TreeFam; TF337594; -.
DR   PRO; PR:P60531; -.
DR   Proteomes; UP000002494; Chromosome 1.
DR   Genevisible; P60531; RN.
DR   GO; GO:0005814; C:centriole; ISO:RGD.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-KW.
DR   GO; GO:0019901; F:protein kinase binding; ISO:RGD.
DR   InterPro; IPR028214; TSKS.
DR   PANTHER; PTHR14351; PTHR14351; 1.
DR   Pfam; PF15358; TSKS; 1.
PE   1: Evidence at protein level;
KW   Cytoplasm; Cytoskeleton; Phosphoprotein; Reference proteome.
FT   CHAIN           1..585
FT                   /note="Testis-specific serine kinase substrate"
FT                   /id="PRO_0000065667"
FT   REGION          91..126
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          262..309
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          559..585
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   MOD_RES         224
FT                   /note="Phosphoserine"
FT                   /evidence="ECO:0007744|PubMed:22673903"
FT   MOD_RES         281
FT                   /note="Phosphoserine"
FT                   /evidence="ECO:0007744|PubMed:22673903"
FT   MOD_RES         309
FT                   /note="Phosphoserine"
FT                   /evidence="ECO:0007744|PubMed:22673903"
SQ   SEQUENCE   585 AA;  63532 MW;  7EBC554C04FA2D14 CRC64;
     MASVVVKTIW QSKEIHEAGD PPAGVESRAQ LVPEAPGGVT SPAKGITKKK KAVSFHGVEP
     RMSHEPMHWC LNLKRSSACT NVSLLNLAAM EPDSSGTDST TEDSGPLALP GPPASPTTPW
     APEDPDITEL LSGVNSGLVR AKDSITSLKE KTTRVNQHVQ TLQSECSVLS ENLERRRQEA
     EELEGYCSQL KGPRPDVLTQ ENCRKVTRSV EDAEIKTNVL KQNSALLEEK LRYLQQQLQD
     ETPRRQEAEL QELEQKLEAG LSRHGLGPTT PIQGCSGPPG SPEEPPRPRG LPSNGWGMAI
     RAGEGPSLSE QELQKVSSGL EELRREVSSL AARWHQEEGA VQEALRLLGG LGGRLDGFLG
     QWERAQREQA QSARGLQELR GRADELCTMV ERSAVSVASL RSELEALGPV KPILEELGRQ
     LQNSRRGPDH VLNLDRPAQG PCPRCASQGQ QLSTESLQQL LERALTPLVD EVKQKGLAPA
     CPSCQRLHKK ILELERQALA KHVRAEALSS TLRLAQDEAV RAKNLLLTDK MKPEEKVATL
     DYMHLKMCSL HDQLSHLPLE GSTGAMGGGS TGGAPPKRGG PGSEQ