ID   TSK_XENTR               Reviewed;         350 AA.
AC   Q5M7S9;
DT   27-JUN-2006, integrated into UniProtKB/Swiss-Prot.
DT   01-FEB-2005, sequence version 1.
DT   03-AUG-2022, entry version 100.
DE   RecName: Full=Tsukushi {ECO:0000250|UniProtKB:Q8WUA8};
DE   AltName: Full=Leucine-rich repeat-containing protein 54;
DE   Flags: Precursor;
GN   Name=tsku; Synonyms=lrcc54, tsk;
OS   Xenopus tropicalis (Western clawed frog) (Silurana tropicalis).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Amphibia;
OC   Batrachia; Anura; Pipoidea; Pipidae; Xenopodinae; Xenopus; Silurana.
OX   NCBI_TaxID=8364;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   TISSUE=Embryo;
RG   NIH - Xenopus Gene Collection (XGC) project;
RL   Submitted (DEC-2004) to the EMBL/GenBank/DDBJ databases.
CC   -!- FUNCTION: Contributes to various developmental events through its
CC       interactions with multiple signaling pathways. Dorsalizing factor which
CC       functions as an inhibitor of bone morphogenetic proteins (BMP) during
CC       gastrulation. Promotes dll1-dependent activation of Notch signaling and
CC       is required for neural crest formation. Induces endoderm and dorsal
CC       mesoderm formation by enhancing nodal2/Xnr2 activity while inhibiting
CC       ventrolateral mesoderm formation through inhibition of fgf8.
CC       {ECO:0000250|UniProtKB:Q65YW8}.
CC   -!- SUBUNIT: Interacts with bmp4. Interacts with dll1 (via extracellular
CC       region). Interacts with fgf8; inhibits fgf8 signaling. Interacts with
CC       nodal2/Xnr2; enhances nodal2 activity. {ECO:0000250|UniProtKB:Q65YW8}.
CC   -!- SUBCELLULAR LOCATION: Secreted {ECO:0000250|UniProtKB:Q65Z91}.
CC   -!- MISCELLANEOUS: This factor is named 'Tsukushi' because its expression
CC       pattern in chick embryos is similar to the shape of the Japanese
CC       horsetail plant, tsukushi. {ECO:0000250|UniProtKB:Q65Z91}.
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DR   EMBL; BC088476; AAH88476.1; -; mRNA.
DR   RefSeq; NP_001011330.1; NM_001011330.1.
DR   AlphaFoldDB; Q5M7S9; -.
DR   SMR; Q5M7S9; -.
DR   STRING; 8364.ENSXETP00000003192; -.
DR   PaxDb; Q5M7S9; -.
DR   DNASU; 496792; -.
DR   GeneID; 496792; -.
DR   KEGG; xtr:496792; -.
DR   CTD; 25987; -.
DR   Xenbase; XB-GENE-493206; tsku.
DR   eggNOG; KOG0619; Eukaryota.
DR   HOGENOM; CLU_785168_0_0_1; -.
DR   InParanoid; Q5M7S9; -.
DR   OMA; SQDVRCR; -.
DR   OrthoDB; 1168051at2759; -.
DR   PhylomeDB; Q5M7S9; -.
DR   Proteomes; UP000008143; Chromosome 2.
DR   Proteomes; UP000790000; Unplaced.
DR   GO; GO:0005615; C:extracellular space; ISS:UniProtKB.
DR   GO; GO:0098868; P:bone growth; ISS:UniProtKB.
DR   GO; GO:0097009; P:energy homeostasis; ISS:UniProtKB.
DR   GO; GO:0003431; P:growth plate cartilage chondrocyte development; ISS:UniProtKB.
DR   GO; GO:0007399; P:nervous system development; IEA:UniProtKB-KW.
DR   Gene3D; 3.80.10.10; -; 2.
DR   InterPro; IPR001611; Leu-rich_rpt.
DR   InterPro; IPR003591; Leu-rich_rpt_typical-subtyp.
DR   InterPro; IPR032675; LRR_dom_sf.
DR   Pfam; PF00560; LRR_1; 1.
DR   Pfam; PF13855; LRR_8; 2.
DR   SMART; SM00369; LRR_TYP; 8.
DR   PROSITE; PS51450; LRR; 9.
PE   2: Evidence at transcript level;
KW   Developmental protein; Glycoprotein; Leucine-rich repeat; Neurogenesis;
KW   Reference proteome; Repeat; Secreted; Signal.
FT   SIGNAL          1..17
FT                   /evidence="ECO:0000255"
FT   CHAIN           18..350
FT                   /note="Tsukushi"
FT                   /id="PRO_0000240412"
FT   DOMAIN          18..59
FT                   /note="LRRNT"
FT   REPEAT          60..81
FT                   /note="LRR 1"
FT   REPEAT          86..107
FT                   /note="LRR 2"
FT   REPEAT          110..131
FT                   /note="LRR 3"
FT   REPEAT          133..154
FT                   /note="LRR 4"
FT   REPEAT          159..180
FT                   /note="LRR 5"
FT   REPEAT          183..203
FT                   /note="LRR 6"
FT   REPEAT          204..225
FT                   /note="LRR 7"
FT   REPEAT          228..250
FT                   /note="LRR 8"
FT   REPEAT          253..275
FT                   /note="LRR 9"
FT   REPEAT          278..299
FT                   /note="LRR 10"
FT   CARBOHYD        75
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255"
FT   CARBOHYD        91
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255"
SQ   SEQUENCE   350 AA;  38444 MW;  A646357A81E9A15B CRC64;
     MAPSWLFLLF IPGMVGSSRS CFPGCQCIVD NFGLFHSFSL TKVDCSGVGP HVVPVSIPLD
     TSYLDLSANG IKRINESVLS GPGYTTLINL NLSHNQIVRI SFSTFSKLRY LESLDLSHNL
     LETLPDGSFL YSRLTELDLS SNKLLEVGIG AFTLKSQGRS MTINLGNNNI RSIHRGAERP
     VPNIHSLTLS GNDLLSVPDL HGIPLRHLDL DRNPLAKIEK ESFLGLEGLT HLSLSDLPNL
     REVSPYSFKT LPSLLDLDLS SNPQLKSLSS DMFFGLDSLQ ELNLAYSGVA ALPKDIMLNL
     PSMKSITWGE NIRCSKTVKE SPFHAQKGRV REEVLLCHDD YGAVPAQDVL