TSLP_MOUSE
ID TSLP_MOUSE Reviewed; 140 AA.
AC Q9JIE6;
DT 11-SEP-2007, integrated into UniProtKB/Swiss-Prot.
DT 01-OCT-2000, sequence version 1.
DT 03-AUG-2022, entry version 107.
DE RecName: Full=Thymic stromal lymphopoietin;
DE AltName: Full=Thymic stroma-derived lymphopoietin;
DE Flags: Precursor;
GN Name=Tslp;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA], SUBCELLULAR LOCATION, AND FUNCTION.
RC STRAIN=BALB/cJ;
RX PubMed=10974033; DOI=10.1084/jem.192.5.671;
RA Sims J.E., Williams D.E., Morrissey P.J., Garka K., Foxworthe D., Price V.,
RA Friend S.L., Farr A., Bedell M.A., Jenkins N.A., Copeland N.G.,
RA Grabstein K., Paxton R.J.;
RT "Molecular cloning and biological characterization of a novel murine
RT lymphoid growth factor.";
RL J. Exp. Med. 192:671-680(2000).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [3]
RP X-RAY CRYSTALLOGRAPHY (1.9 ANGSTROMS) OF 20-140 IN COMPLEX WITH IL7R AND
RP CRLF2, SUBUNIT, AND DISULFIDE BONDS.
RX PubMed=24632570; DOI=10.1038/nsmb.2794;
RA Verstraete K., van Schie L., Vyncke L., Bloch Y., Tavernier J., Pauwels E.,
RA Peelman F., Savvides S.N.;
RT "Structural basis of the proinflammatory signaling complex mediated by
RT TSLP.";
RL Nat. Struct. Mol. Biol. 21:375-382(2014).
CC -!- FUNCTION: Cytokine that induces the release of T-cell-attracting
CC chemokines from monocytes and, in particular, enhances the maturation
CC of CD11c(+) dendritic cells. Can induce allergic inflammation by
CC directly activating mast cells (By similarity). {ECO:0000250,
CC ECO:0000269|PubMed:10974033}.
CC -!- SUBUNIT: Interacts with a receptor composed of CRLF2 and IL7R. Binding
CC of TSLP to CRLF2/TSLPR is a mechanistic prerequisite for recruitment of
CC IL7R to the high-affinity ternary complex.
CC {ECO:0000269|PubMed:24632570}.
CC -!- INTERACTION:
CC Q9JIE6; Q8CII9: Crlf2; NbExp=9; IntAct=EBI-16096402, EBI-15887886;
CC -!- SUBCELLULAR LOCATION: Secreted {ECO:0000269|PubMed:10974033}.
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DR EMBL; AF232937; AAF81677.1; -; mRNA.
DR EMBL; BC130230; AAI30231.1; -; mRNA.
DR CCDS; CCDS29121.1; -.
DR RefSeq; NP_067342.1; NM_021367.2.
DR PDB; 4NN5; X-ray; 1.90 A; A=20-140.
DR PDB; 4NN6; X-ray; 2.54 A; A=20-140.
DR PDB; 4NN7; X-ray; 3.78 A; A=20-140.
DR PDBsum; 4NN5; -.
DR PDBsum; 4NN6; -.
DR PDBsum; 4NN7; -.
DR AlphaFoldDB; Q9JIE6; -.
DR SMR; Q9JIE6; -.
DR DIP; DIP-60842N; -.
DR IntAct; Q9JIE6; 2.
DR STRING; 10090.ENSMUSP00000025237; -.
DR GlyGen; Q9JIE6; 3 sites.
DR PhosphoSitePlus; Q9JIE6; -.
DR PaxDb; Q9JIE6; -.
DR PRIDE; Q9JIE6; -.
DR Antibodypedia; 25309; 676 antibodies from 41 providers.
DR DNASU; 53603; -.
DR Ensembl; ENSMUST00000025237; ENSMUSP00000025237; ENSMUSG00000024379.
DR GeneID; 53603; -.
DR KEGG; mmu:53603; -.
DR UCSC; uc008ejl.2; mouse.
DR CTD; 85480; -.
DR MGI; MGI:1855696; Tslp.
DR VEuPathDB; HostDB:ENSMUSG00000024379; -.
DR eggNOG; ENOG502TEM0; Eukaryota.
DR GeneTree; ENSGT00390000012541; -.
DR HOGENOM; CLU_143224_0_0_1; -.
DR InParanoid; Q9JIE6; -.
DR OMA; NPTPRCA; -.
DR OrthoDB; 1389326at2759; -.
DR PhylomeDB; Q9JIE6; -.
DR TreeFam; TF338216; -.
DR Reactome; R-MMU-1266695; Interleukin-7 signaling.
DR BioGRID-ORCS; 53603; 1 hit in 70 CRISPR screens.
DR PRO; PR:Q9JIE6; -.
DR Proteomes; UP000000589; Chromosome 18.
DR RNAct; Q9JIE6; protein.
DR Bgee; ENSMUSG00000024379; Expressed in ureter smooth muscle and 75 other tissues.
DR Genevisible; Q9JIE6; MM.
DR GO; GO:0005576; C:extracellular region; ISO:MGI.
DR GO; GO:0005615; C:extracellular space; IEA:UniProtKB-KW.
DR GO; GO:0005125; F:cytokine activity; IDA:MGI.
DR GO; GO:0005139; F:interleukin-7 receptor binding; ISO:MGI.
DR GO; GO:0061844; P:antimicrobial humoral immune response mediated by antimicrobial peptide; ISO:MGI.
DR GO; GO:0050832; P:defense response to fungus; ISO:MGI.
DR GO; GO:0050829; P:defense response to Gram-negative bacterium; ISO:MGI.
DR GO; GO:0043066; P:negative regulation of apoptotic process; ISO:MGI.
DR GO; GO:0008284; P:positive regulation of cell population proliferation; ISO:MGI.
DR GO; GO:0071654; P:positive regulation of chemokine (C-C motif) ligand 1 production; ISO:MGI.
DR GO; GO:0032722; P:positive regulation of chemokine production; ISO:MGI.
DR GO; GO:0001961; P:positive regulation of cytokine-mediated signaling pathway; ISO:MGI.
DR GO; GO:0071657; P:positive regulation of granulocyte colony-stimulating factor production; ISO:MGI.
DR GO; GO:0050729; P:positive regulation of inflammatory response; ISO:MGI.
DR GO; GO:0032733; P:positive regulation of interleukin-10 production; ISO:MGI.
DR GO; GO:0032736; P:positive regulation of interleukin-13 production; ISO:MGI.
DR GO; GO:0032754; P:positive regulation of interleukin-5 production; ISO:MGI.
DR GO; GO:0032755; P:positive regulation of interleukin-6 production; ISO:MGI.
DR GO; GO:0033005; P:positive regulation of mast cell activation; ISO:MGI.
DR GO; GO:1904894; P:positive regulation of receptor signaling pathway via STAT; ISO:MGI.
DR GO; GO:0042531; P:positive regulation of tyrosine phosphorylation of STAT protein; ISO:MGI.
DR Gene3D; 1.20.1250.90; -; 1.
DR InterPro; IPR029189; TSLP.
DR InterPro; IPR038329; TSLP_sf.
DR PANTHER; PTHR38003; PTHR38003; 1.
DR Pfam; PF15216; TSLP; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Cytokine; Disulfide bond; Glycoprotein; Reference proteome;
KW Secreted; Signal.
FT SIGNAL 1..19
FT /evidence="ECO:0000255"
FT CHAIN 20..140
FT /note="Thymic stromal lymphopoietin"
FT /id="PRO_0000300874"
FT SITE 37
FT /note="Inserts into a conserved IL7R hydrophobic pocket,
FT important for IL7R-binding"
FT /evidence="ECO:0000269|PubMed:24632570"
FT CARBOHYD 21
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 26
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 123
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT DISULFID 25..98
FT /evidence="ECO:0000269|PubMed:24632570"
FT DISULFID 57..63
FT /evidence="ECO:0000269|PubMed:24632570"
FT DISULFID 78..121
FT /evidence="ECO:0000269|PubMed:24632570"
FT HELIX 22..24
FT /evidence="ECO:0007829|PDB:4NN5"
FT HELIX 27..36
FT /evidence="ECO:0007829|PDB:4NN5"
FT HELIX 38..46
FT /evidence="ECO:0007829|PDB:4NN5"
FT HELIX 60..72
FT /evidence="ECO:0007829|PDB:4NN5"
FT HELIX 81..83
FT /evidence="ECO:0007829|PDB:4NN5"
FT HELIX 85..97
FT /evidence="ECO:0007829|PDB:4NN5"
FT HELIX 118..137
FT /evidence="ECO:0007829|PDB:4NN5"
SQ SEQUENCE 140 AA; 16152 MW; 5B6D3196E73BAA8B CRC64;
MVLLRSLFIL QVLVRMGLTY NFSNCNFTSI TKIYCNIIFH DLTGDLKGAK FEQIEDCESK
PACLLKIEYY TLNPIPGCPS LPDKTFARRT REALNDHCPG YPETERNDGT QEMAQEVQNI
CLNQTSQILR LWYSFMQSPE
