TSN1_HUMAN
ID TSN1_HUMAN Reviewed; 241 AA.
AC O60635; D3DQ14; O60745; Q5VST0;
DT 01-DEC-2000, integrated into UniProtKB/Swiss-Prot.
DT 01-DEC-2000, sequence version 2.
DT 03-AUG-2022, entry version 162.
DE RecName: Full=Tetraspanin-1;
DE Short=Tspan-1;
DE AltName: Full=Tetraspan NET-1;
DE AltName: Full=Tetraspanin TM4-C;
GN Name=TSPAN1;
OS Homo sapiens (Human).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae;
OC Homo.
OX NCBI_TaxID=9606;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA].
RX PubMed=9714763; DOI=10.1016/s0167-4781(98)00087-6;
RA Todd S.C., Doctor V.S., Levy S.;
RT "Sequences and expression of six new members of the tetraspanin/TM4SF
RT family.";
RL Biochim. Biophys. Acta 1399:101-104(1998).
RN [2]
RP NUCLEOTIDE SEQUENCE [MRNA].
RA Rubinstein E., Serru V., Boucheix C.;
RT "New tetraspans identified in the EST database.";
RL Submitted (MAY-1998) to the EMBL/GenBank/DDBJ databases.
RN [3]
RP NUCLEOTIDE SEQUENCE [MRNA].
RA Puls K.L., Ni J., Liu D., Morahan G., Wright M.D.;
RT "The molecular characterization of four tetraspanins.";
RL Submitted (MAR-1999) to the EMBL/GenBank/DDBJ databases.
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC TISSUE=Testis;
RX PubMed=14702039; DOI=10.1038/ng1285;
RA Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R.,
RA Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H.,
RA Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.,
RA Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K.,
RA Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H.,
RA Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M.,
RA Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K.,
RA Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T.,
RA Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M.,
RA Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S.,
RA Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H.,
RA Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K.,
RA Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N.,
RA Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S.,
RA Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O.,
RA Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H.,
RA Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B.,
RA Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y.,
RA Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K.,
RA Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T.,
RA Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T.,
RA Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y.,
RA Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H.,
RA Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y.,
RA Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H.,
RA Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O.,
RA Isogai T., Sugano S.;
RT "Complete sequencing and characterization of 21,243 full-length human
RT cDNAs.";
RL Nat. Genet. 36:40-45(2004).
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX PubMed=16710414; DOI=10.1038/nature04727;
RA Gregory S.G., Barlow K.F., McLay K.E., Kaul R., Swarbreck D., Dunham A.,
RA Scott C.E., Howe K.L., Woodfine K., Spencer C.C.A., Jones M.C., Gillson C.,
RA Searle S., Zhou Y., Kokocinski F., McDonald L., Evans R., Phillips K.,
RA Atkinson A., Cooper R., Jones C., Hall R.E., Andrews T.D., Lloyd C.,
RA Ainscough R., Almeida J.P., Ambrose K.D., Anderson F., Andrew R.W.,
RA Ashwell R.I.S., Aubin K., Babbage A.K., Bagguley C.L., Bailey J.,
RA Beasley H., Bethel G., Bird C.P., Bray-Allen S., Brown J.Y., Brown A.J.,
RA Buckley D., Burton J., Bye J., Carder C., Chapman J.C., Clark S.Y.,
RA Clarke G., Clee C., Cobley V., Collier R.E., Corby N., Coville G.J.,
RA Davies J., Deadman R., Dunn M., Earthrowl M., Ellington A.G., Errington H.,
RA Frankish A., Frankland J., French L., Garner P., Garnett J., Gay L.,
RA Ghori M.R.J., Gibson R., Gilby L.M., Gillett W., Glithero R.J.,
RA Grafham D.V., Griffiths C., Griffiths-Jones S., Grocock R., Hammond S.,
RA Harrison E.S.I., Hart E., Haugen E., Heath P.D., Holmes S., Holt K.,
RA Howden P.J., Hunt A.R., Hunt S.E., Hunter G., Isherwood J., James R.,
RA Johnson C., Johnson D., Joy A., Kay M., Kershaw J.K., Kibukawa M.,
RA Kimberley A.M., King A., Knights A.J., Lad H., Laird G., Lawlor S.,
RA Leongamornlert D.A., Lloyd D.M., Loveland J., Lovell J., Lush M.J.,
RA Lyne R., Martin S., Mashreghi-Mohammadi M., Matthews L., Matthews N.S.W.,
RA McLaren S., Milne S., Mistry S., Moore M.J.F., Nickerson T., O'Dell C.N.,
RA Oliver K., Palmeiri A., Palmer S.A., Parker A., Patel D., Pearce A.V.,
RA Peck A.I., Pelan S., Phelps K., Phillimore B.J., Plumb R., Rajan J.,
RA Raymond C., Rouse G., Saenphimmachak C., Sehra H.K., Sheridan E.,
RA Shownkeen R., Sims S., Skuce C.D., Smith M., Steward C., Subramanian S.,
RA Sycamore N., Tracey A., Tromans A., Van Helmond Z., Wall M., Wallis J.M.,
RA White S., Whitehead S.L., Wilkinson J.E., Willey D.L., Williams H.,
RA Wilming L., Wray P.W., Wu Z., Coulson A., Vaudin M., Sulston J.E.,
RA Durbin R.M., Hubbard T., Wooster R., Dunham I., Carter N.P., McVean G.,
RA Ross M.T., Harrow J., Olson M.V., Beck S., Rogers J., Bentley D.R.;
RT "The DNA sequence and biological annotation of human chromosome 1.";
RL Nature 441:315-321(2006).
RN [6]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RA Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M.,
RA Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J.,
RA Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S.,
RA Turner R., Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H.,
RA Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K.,
RA Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D.,
RA Hunkapiller M.W., Myers E.W., Venter J.C.;
RL Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases.
RN [7]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC TISSUE=Colon;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [8]
RP SUBCELLULAR LOCATION, GLYCOSYLATION AT ASN-141; ASN-154; ASN-178 AND
RP ASN-184, AND MUTAGENESIS OF ASN-141; ASN-154; ASN-178 AND ASN-184.
RX PubMed=19508227; DOI=10.2174/092986609789071234;
RA Scholz C.-J., Sauer G., Deissler H.;
RT "Glycosylation of tetraspanin Tspan-1 at four distinct sites promotes its
RT transition through the endoplasmic reticulum.";
RL Protein Pept. Lett. 16:1244-1248(2009).
CC -!- INTERACTION:
CC O60635; O60779: SLC19A2; NbExp=5; IntAct=EBI-3914312, EBI-3941998;
CC O60635; Q00059: TFAM; NbExp=3; IntAct=EBI-3914312, EBI-1049924;
CC O60635; Q96HE8: TMEM80; NbExp=3; IntAct=EBI-3914312, EBI-11742770;
CC -!- SUBCELLULAR LOCATION: Lysosome membrane {ECO:0000269|PubMed:19508227};
CC Multi-pass membrane protein {ECO:0000269|PubMed:19508227}.
CC -!- SIMILARITY: Belongs to the tetraspanin (TM4SF) family. {ECO:0000305}.
CC -!- WEB RESOURCE: Name=Atlas of Genetics and Cytogenetics in Oncology and
CC Haematology;
CC URL="http://atlasgeneticsoncology.org/Genes/TSPAN1ID44178ch1p34.html";
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DR EMBL; AF054838; AAC69714.1; -; mRNA.
DR EMBL; AF065388; AAC17119.1; -; mRNA.
DR EMBL; AF133425; AAF08364.1; -; mRNA.
DR EMBL; AK313774; BAG36512.1; -; mRNA.
DR EMBL; AL672043; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; CH471059; EAX06938.1; -; Genomic_DNA.
DR EMBL; CH471059; EAX06939.1; -; Genomic_DNA.
DR EMBL; CH471059; EAX06940.1; -; Genomic_DNA.
DR EMBL; BC007290; AAH07290.1; -; mRNA.
DR EMBL; BC013404; AAH13404.1; -; mRNA.
DR CCDS; CCDS530.1; -.
DR PIR; A59262; A59262.
DR RefSeq; NP_005718.2; NM_005727.3.
DR AlphaFoldDB; O60635; -.
DR SMR; O60635; -.
DR BioGRID; 115410; 40.
DR IntAct; O60635; 4.
DR STRING; 9606.ENSP00000361072; -.
DR GlyConnect; 1797; 9 N-Linked glycans (2 sites).
DR GlyGen; O60635; 4 sites, 10 N-linked glycans (2 sites).
DR iPTMnet; O60635; -.
DR PhosphoSitePlus; O60635; -.
DR BioMuta; TSPAN1; -.
DR EPD; O60635; -.
DR jPOST; O60635; -.
DR MassIVE; O60635; -.
DR MaxQB; O60635; -.
DR PaxDb; O60635; -.
DR PeptideAtlas; O60635; -.
DR PRIDE; O60635; -.
DR ProteomicsDB; 49488; -.
DR Antibodypedia; 32752; 278 antibodies from 31 providers.
DR DNASU; 10103; -.
DR Ensembl; ENST00000372003.6; ENSP00000361072.1; ENSG00000117472.10.
DR GeneID; 10103; -.
DR KEGG; hsa:10103; -.
DR MANE-Select; ENST00000372003.6; ENSP00000361072.1; NM_005727.4; NP_005718.2.
DR UCSC; uc001cpd.4; human.
DR CTD; 10103; -.
DR DisGeNET; 10103; -.
DR GeneCards; TSPAN1; -.
DR HGNC; HGNC:20657; TSPAN1.
DR HPA; ENSG00000117472; Tissue enhanced (intestine).
DR MalaCards; TSPAN1; -.
DR MIM; 613170; gene.
DR neXtProt; NX_O60635; -.
DR OpenTargets; ENSG00000117472; -.
DR PharmGKB; PA134938100; -.
DR VEuPathDB; HostDB:ENSG00000117472; -.
DR eggNOG; KOG3882; Eukaryota.
DR GeneTree; ENSGT00940000158851; -.
DR HOGENOM; CLU_055524_4_1_1; -.
DR InParanoid; O60635; -.
DR OMA; HKESKCL; -.
DR PhylomeDB; O60635; -.
DR TreeFam; TF352892; -.
DR PathwayCommons; O60635; -.
DR SignaLink; O60635; -.
DR BioGRID-ORCS; 10103; 10 hits in 1066 CRISPR screens.
DR ChiTaRS; TSPAN1; human.
DR GenomeRNAi; 10103; -.
DR Pharos; O60635; Tbio.
DR PRO; PR:O60635; -.
DR Proteomes; UP000005640; Chromosome 1.
DR RNAct; O60635; protein.
DR Bgee; ENSG00000117472; Expressed in bronchial epithelial cell and 128 other tissues.
DR Genevisible; O60635; HS.
DR GO; GO:0030054; C:cell junction; IDA:HPA.
DR GO; GO:0005737; C:cytoplasm; IDA:UniProtKB.
DR GO; GO:0070062; C:extracellular exosome; HDA:UniProtKB.
DR GO; GO:0005887; C:integral component of plasma membrane; IBA:GO_Central.
DR GO; GO:0043231; C:intracellular membrane-bounded organelle; IDA:HPA.
DR GO; GO:0005765; C:lysosomal membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0016020; C:membrane; IDA:UniProtKB.
DR GO; GO:0005654; C:nucleoplasm; IDA:HPA.
DR GO; GO:0048471; C:perinuclear region of cytoplasm; IDA:UniProtKB.
DR GO; GO:0005886; C:plasma membrane; IDA:UniProtKB.
DR GO; GO:0031982; C:vesicle; IDA:UniProtKB.
DR GO; GO:0050821; P:protein stabilization; IDA:UniProtKB.
DR Gene3D; 1.10.1450.10; -; 1.
DR InterPro; IPR018499; Tetraspanin/Peripherin.
DR InterPro; IPR000301; Tetraspanin_animals.
DR InterPro; IPR018503; Tetraspanin_CS.
DR InterPro; IPR008952; Tetraspanin_EC2_sf.
DR PANTHER; PTHR19282; PTHR19282; 1.
DR Pfam; PF00335; Tetraspanin; 1.
DR PIRSF; PIRSF002419; Tetraspanin; 1.
DR SUPFAM; SSF48652; SSF48652; 1.
DR PROSITE; PS00421; TM4_1; 1.
PE 1: Evidence at protein level;
KW Glycoprotein; Lysosome; Membrane; Reference proteome; Transmembrane;
KW Transmembrane helix.
FT CHAIN 1..241
FT /note="Tetraspanin-1"
FT /id="PRO_0000219234"
FT TOPO_DOM 1..11
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 12..32
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 33..52
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 53..73
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 74..88
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 89..109
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 110..211
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 212..232
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 233..241
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT CARBOHYD 141
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000269|PubMed:19508227"
FT CARBOHYD 154
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000269|PubMed:19508227"
FT CARBOHYD 178
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000269|PubMed:19508227"
FT CARBOHYD 184
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000269|PubMed:19508227"
FT VARIANT 38
FT /note="S -> F (in dbSNP:rs2234267)"
FT /id="VAR_052327"
FT VARIANT 87
FT /note="V -> M (in dbSNP:rs2234268)"
FT /id="VAR_034573"
FT MUTAGEN 141
FT /note="N->A: Loss of glycosylation."
FT /evidence="ECO:0000269|PubMed:19508227"
FT MUTAGEN 154
FT /note="N->A: Loss of glycosylation."
FT /evidence="ECO:0000269|PubMed:19508227"
FT MUTAGEN 178
FT /note="N->A: Loss of glycosylation."
FT /evidence="ECO:0000269|PubMed:19508227"
FT MUTAGEN 184
FT /note="N->A: Loss of glycosylation."
FT /evidence="ECO:0000269|PubMed:19508227"
FT CONFLICT 189
FT /note="K -> E (in Ref. 1; AAC69714)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 241 AA; 26301 MW; AF938AD7147CB884 CRC64;
MQCFSFIKTM MILFNLLIFL CGAALLAVGI WVSIDGASFL KIFGPLSSSA MQFVNVGYFL
IAAGVVVFAL GFLGCYGAKT ESKCALVTFF FILLLIFIAE VAAAVVALVY TTMAEHFLTL
LVVPAIKKDY GSQEDFTQVW NTTMKGLKCC GFTNYTDFED SPYFKENSAF PPFCCNDNVT
NTANETCTKQ KAHDQKVEGC FNQLLYDIRT NAVTVGGVAA GIGGLELAAM IVSMYLYCNL
Q
