TSN2A_DROME
ID TSN2A_DROME Reviewed; 244 AA.
AC O46101;
DT 05-OCT-2016, integrated into UniProtKB/Swiss-Prot.
DT 01-JUN-1998, sequence version 1.
DT 03-AUG-2022, entry version 155.
DE RecName: Full=Tetraspanin-2A {ECO:0000312|FlyBase:FBgn0024361};
GN Name=Tsp2A {ECO:0000312|FlyBase:FBgn0024361};
GN ORFNames=CG11415 {ECO:0000312|FlyBase:FBgn0024361};
OS Drosophila melanogaster (Fruit fly).
OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea;
OC Drosophilidae; Drosophila; Sophophora.
OX NCBI_TaxID=7227 {ECO:0000312|Proteomes:UP000000803};
RN [1] {ECO:0000312|Proteomes:UP000000803}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Berkeley;
RX PubMed=10731132; DOI=10.1126/science.287.5461.2185;
RA Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D.,
RA Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F.,
RA George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N.,
RA Sutton G.G., Wortman J.R., Yandell M.D., Zhang Q., Chen L.X., Brandon R.C.,
RA Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C.,
RA Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A.,
RA An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A.,
RA Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V.,
RA Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J.,
RA Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E.,
RA Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B.,
RA Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I.,
RA Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C.,
RA Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S.,
RA Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M.,
RA Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M.,
RA Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D.,
RA Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F.,
RA Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D.,
RA Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A.,
RA Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C.,
RA McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C.,
RA Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L.,
RA Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R.,
RA Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V.,
RA Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F.,
RA Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J.,
RA Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R.,
RA Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y.,
RA Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T.,
RA Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S.,
RA Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W.,
RA Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M.,
RA Venter J.C.;
RT "The genome sequence of Drosophila melanogaster.";
RL Science 287:2185-2195(2000).
RN [2] {ECO:0000312|Proteomes:UP000000803}
RP GENOME REANNOTATION.
RC STRAIN=Berkeley {ECO:0000312|Proteomes:UP000000803};
RX PubMed=12537572; DOI=10.1186/gb-2002-3-12-research0083;
RA Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S.,
RA Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E.,
RA Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P.,
RA Bettencourt B.R., Celniker S.E., de Grey A.D.N.J., Drysdale R.A.,
RA Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M.,
RA Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E.;
RT "Annotation of the Drosophila melanogaster euchromatic genome: a systematic
RT review.";
RL Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002).
RN [3] {ECO:0000312|EMBL:CAA17687.1}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Oregon-R {ECO:0000312|EMBL:CAA17687.1};
RX PubMed=10731137; DOI=10.1126/science.287.5461.2220;
RA Benos P.V., Gatt M.K., Ashburner M., Murphy L., Harris D., Barrell B.G.,
RA Ferraz C., Vidal S., Brun C., Demailles J., Cadieu E., Dreano S., Gloux S.,
RA Lelaure V., Mottier S., Galibert F., Borkova D., Minana B., Kafatos F.C.,
RA Louis C., Siden-Kiamos I., Bolshakov S., Papagiannakis G., Spanos L.,
RA Cox S., Madueno E., de Pablos B., Modolell J., Peter A., Schoettler P.,
RA Werner M., Mourkioti F., Beinert N., Dowe G., Schaefer U., Jaeckle H.,
RA Bucheton A., Callister D.M., Campbell L.A., Darlamitsou A., Henderson N.S.,
RA McMillan P.J., Salles C., Tait E.A., Valenti P., Saunders R.D.C.,
RA Glover D.M.;
RT "From sequence to chromosome: the tip of the X chromosome of D.
RT melanogaster.";
RL Science 287:2220-2222(2000).
RN [4] {ECO:0000312|EMBL:AAT47766.1}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=Berkeley {ECO:0000312|EMBL:AAT47766.1};
RA Stapleton M., Carlson J., Chavez C., Frise E., George R., Pacleb J.,
RA Park S., Wan K., Yu C., Rubin G.M., Celniker S.;
RL Submitted (JUN-2004) to the EMBL/GenBank/DDBJ databases.
RN [5] {ECO:0000305}
RP FUNCTION, IDENTIFICATION IN A COMPLEX WITH SSK AND MESH, SUBCELLULAR
RP LOCATION, AND DEVELOPMENTAL STAGE.
RX PubMed=26848177; DOI=10.1242/jcs.180448;
RA Izumi Y., Motoishi M., Furuse K., Furuse M.;
RT "A tetraspanin regulates septate junction formation in Drosophila midgut.";
RL J. Cell Sci. 129:1155-1164(2016).
CC -!- FUNCTION: Required for assembly of smooth septate junctions (sSJs),
CC together with Ssk and mesh. Important for barrier function of the
CC midgut epithelium. {ECO:0000269|PubMed:26848177}.
CC -!- SUBUNIT: Forms a complex with Ssk and mesh.
CC {ECO:0000269|PubMed:26848177}.
CC -!- SUBCELLULAR LOCATION: Apicolateral cell membrane
CC {ECO:0000269|PubMed:26848177}; Multi-pass membrane protein
CC {ECO:0000255}. Cell junction, septate junction
CC {ECO:0000269|PubMed:26848177}.
CC -!- DEVELOPMENTAL STAGE: Detected in embryos from stage 15 onwards (at
CC protein level). In first instar larvae, detected in midgut, Malpighian
CC tubules and outer epithelial layer of the proventriculus (OELP) (at
CC protein level). {ECO:0000269|PubMed:26848177}.
CC -!- SIMILARITY: Belongs to the tetraspanin (TM4SF) family.
CC {ECO:0000255|RuleBase:RU361218}.
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DR EMBL; AE014298; AAF45608.1; -; Genomic_DNA.
DR EMBL; AE014298; ADV37607.1; -; Genomic_DNA.
DR EMBL; AL022018; CAA17687.1; -; Genomic_DNA.
DR EMBL; BT014915; AAT47766.1; -; mRNA.
DR PIR; T13615; T13615.
DR RefSeq; NP_001188523.1; NM_001201594.2.
DR RefSeq; NP_525037.1; NM_080298.4.
DR AlphaFoldDB; O46101; -.
DR SMR; O46101; -.
DR STRING; 7227.FBpp0070204; -.
DR PaxDb; O46101; -.
DR PRIDE; O46101; -.
DR EnsemblMetazoa; FBtr0070211; FBpp0070204; FBgn0024361.
DR EnsemblMetazoa; FBtr0303284; FBpp0292376; FBgn0024361.
DR GeneID; 31080; -.
DR KEGG; dme:Dmel_CG11415; -.
DR UCSC; CG11415-RA; d. melanogaster.
DR CTD; 31080; -.
DR FlyBase; FBgn0024361; Tsp2A.
DR VEuPathDB; VectorBase:FBgn0024361; -.
DR eggNOG; KOG3882; Eukaryota.
DR HOGENOM; CLU_055524_8_0_1; -.
DR InParanoid; O46101; -.
DR OMA; EFYIGIY; -.
DR OrthoDB; 1205716at2759; -.
DR PhylomeDB; O46101; -.
DR Reactome; R-DME-6798695; Neutrophil degranulation.
DR BioGRID-ORCS; 31080; 0 hits in 1 CRISPR screen.
DR ChiTaRS; Tsp2A; fly.
DR GenomeRNAi; 31080; -.
DR PRO; PR:O46101; -.
DR Proteomes; UP000000803; Chromosome X.
DR Bgee; FBgn0024361; Expressed in adult midgut (Drosophila) and 29 other tissues.
DR GO; GO:0016327; C:apicolateral plasma membrane; IDA:UniProtKB.
DR GO; GO:0016021; C:integral component of membrane; ISM:FlyBase.
DR GO; GO:0005887; C:integral component of plasma membrane; IBA:GO_Central.
DR GO; GO:0005920; C:smooth septate junction; IDA:UniProtKB.
DR GO; GO:0061028; P:establishment of endothelial barrier; IMP:UniProtKB.
DR GO; GO:0008347; P:glial cell migration; IGI:FlyBase.
DR GO; GO:0090528; P:smooth septate junction assembly; IMP:UniProtKB.
DR Gene3D; 1.10.1450.10; -; 1.
DR InterPro; IPR018499; Tetraspanin/Peripherin.
DR InterPro; IPR000301; Tetraspanin_animals.
DR InterPro; IPR008952; Tetraspanin_EC2_sf.
DR PANTHER; PTHR19282; PTHR19282; 1.
DR Pfam; PF00335; Tetraspanin; 1.
DR PIRSF; PIRSF002419; Tetraspanin; 1.
DR SUPFAM; SSF48652; SSF48652; 1.
PE 1: Evidence at protein level;
KW Cell junction; Cell membrane; Membrane; Reference proteome; Transmembrane;
KW Transmembrane helix.
FT CHAIN 1..244
FT /note="Tetraspanin-2A"
FT /id="PRO_0000437455"
FT TOPO_DOM 1..22
FT /note="Cytoplasmic"
FT /evidence="ECO:0000305"
FT TRANSMEM 23..43
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 44..61
FT /note="Extracellular"
FT /evidence="ECO:0000305"
FT TRANSMEM 62..82
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 83..91
FT /note="Cytoplasmic"
FT /evidence="ECO:0000305"
FT TRANSMEM 92..112
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 113..206
FT /note="Extracellular"
FT /evidence="ECO:0000305"
FT TRANSMEM 207..227
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 228..244
FT /note="Cytoplasmic"
FT /evidence="ECO:0000305"
SQ SEQUENCE 244 AA; 26894 MW; 216392DADB9EA284 CRC64;
MGIGYGASDE QLEKQIGCVK YTLFCFNIVA WMISTALFAL TVWLRAEPGF NDWLRILEAQ
SFYIGVYVLI GISIVMMAVS FLGCLSALME NTLALFVFVG TQVFGFIAIV AGSAVLLQFS
TINSSLQPLL NVSLRGFVAT SEYTYSNYVL TMIQENIGCC GATGPWDYLD LRQPLPSSCR
DTVSGNAFFN GCVDELTWFF EGKTGWIVAL AMTLGLLNVI CAVMSFVLVQ AVKKEEEQAS
NYRR
