TSN2_MOUSE
ID TSN2_MOUSE Reviewed; 221 AA.
AC Q922J6; Q543K4; Q9D397;
DT 19-SEP-2002, integrated into UniProtKB/Swiss-Prot.
DT 01-DEC-2001, sequence version 1.
DT 03-AUG-2022, entry version 147.
DE RecName: Full=Tetraspanin-2;
DE Short=Tspan-2;
GN Name=Tspan2;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=C57BL/6J; TISSUE=Hypothalamus, and Medulla oblongata;
RX PubMed=16141072; DOI=10.1126/science.1112014;
RA Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N.,
RA Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K.,
RA Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.,
RA Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R.,
RA Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T.,
RA Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A.,
RA Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B.,
RA Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M.,
RA Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S.,
RA Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E.,
RA Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D.,
RA Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M.,
RA Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H.,
RA Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V.,
RA Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S.,
RA Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H.,
RA Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N.,
RA Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F.,
RA Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G.,
RA Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z.,
RA Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C.,
RA Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y.,
RA Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S.,
RA Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K.,
RA Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R.,
RA van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H.,
RA Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M.,
RA Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C.,
RA Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S.,
RA Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K.,
RA Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M.,
RA Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C.,
RA Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A.,
RA Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.;
RT "The transcriptional landscape of the mammalian genome.";
RL Science 309:1559-1563(2005).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [3]
RP PROTEIN SEQUENCE OF 155-166, AND IDENTIFICATION BY MASS SPECTROMETRY.
RC STRAIN=C57BL/6J; TISSUE=Brain;
RA Lubec G., Kang S.U.;
RL Submitted (APR-2007) to UniProtKB.
RN [4]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Brain;
RX PubMed=21183079; DOI=10.1016/j.cell.2010.12.001;
RA Huttlin E.L., Jedrychowski M.P., Elias J.E., Goswami T., Rad R.,
RA Beausoleil S.A., Villen J., Haas W., Sowa M.E., Gygi S.P.;
RT "A tissue-specific atlas of mouse protein phosphorylation and expression.";
RL Cell 143:1174-1189(2010).
CC -!- FUNCTION: May play a role in signalling in oligodendrocytes in the
CC early stages of their terminal differentiation into myelin-forming glia
CC and may also function in stabilizing the mature sheath. {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Membrane {ECO:0000305}; Multi-pass membrane
CC protein {ECO:0000305}.
CC -!- SIMILARITY: Belongs to the tetraspanin (TM4SF) family. {ECO:0000305}.
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DR EMBL; AK018178; BAB31109.1; -; mRNA.
DR EMBL; AK032520; BAC27907.1; -; mRNA.
DR EMBL; AK049888; BAC33972.1; -; mRNA.
DR EMBL; AK079560; BAC37684.1; -; mRNA.
DR EMBL; BC007185; AAH07185.1; -; mRNA.
DR CCDS; CCDS17688.1; -.
DR RefSeq; NP_081809.2; NM_027533.3.
DR AlphaFoldDB; Q922J6; -.
DR SMR; Q922J6; -.
DR BioGRID; 214232; 1.
DR STRING; 10090.ENSMUSP00000029451; -.
DR GlyConnect; 2760; 13 N-Linked glycans (1 site).
DR GlyGen; Q922J6; 1 site, 13 N-linked glycans (1 site).
DR iPTMnet; Q922J6; -.
DR PhosphoSitePlus; Q922J6; -.
DR SwissPalm; Q922J6; -.
DR MaxQB; Q922J6; -.
DR PaxDb; Q922J6; -.
DR PRIDE; Q922J6; -.
DR ProteomicsDB; 297986; -.
DR Antibodypedia; 2866; 246 antibodies from 26 providers.
DR DNASU; 70747; -.
DR Ensembl; ENSMUST00000029451; ENSMUSP00000029451; ENSMUSG00000027858.
DR GeneID; 70747; -.
DR KEGG; mmu:70747; -.
DR UCSC; uc008qrw.2; mouse.
DR CTD; 10100; -.
DR MGI; MGI:1917997; Tspan2.
DR VEuPathDB; HostDB:ENSMUSG00000027858; -.
DR eggNOG; KOG3882; Eukaryota.
DR GeneTree; ENSGT00940000157504; -.
DR HOGENOM; CLU_055524_10_1_1; -.
DR InParanoid; Q922J6; -.
DR OMA; VNRTIYH; -.
DR OrthoDB; 1205716at2759; -.
DR PhylomeDB; Q922J6; -.
DR TreeFam; TF352895; -.
DR BioGRID-ORCS; 70747; 2 hits in 71 CRISPR screens.
DR ChiTaRS; Tspan2; mouse.
DR PRO; PR:Q922J6; -.
DR Proteomes; UP000000589; Chromosome 3.
DR RNAct; Q922J6; protein.
DR Bgee; ENSMUSG00000027858; Expressed in ventral tegmental area and 221 other tissues.
DR ExpressionAtlas; Q922J6; baseline and differential.
DR Genevisible; Q922J6; MM.
DR GO; GO:0005887; C:integral component of plasma membrane; IBA:GO_Central.
DR GO; GO:0043209; C:myelin sheath; IDA:MGI.
DR GO; GO:0005654; C:nucleoplasm; ISO:MGI.
DR GO; GO:0005886; C:plasma membrane; IDA:MGI.
DR GO; GO:0014002; P:astrocyte development; IMP:MGI.
DR GO; GO:0061564; P:axon development; IGI:MGI.
DR GO; GO:0007420; P:brain development; IEA:Ensembl.
DR GO; GO:0006954; P:inflammatory response; IGI:MGI.
DR GO; GO:0014005; P:microglia development; IMP:MGI.
DR GO; GO:0042552; P:myelination; IGI:MGI.
DR GO; GO:0048709; P:oligodendrocyte differentiation; IMP:MGI.
DR Gene3D; 1.10.1450.10; -; 1.
DR InterPro; IPR018499; Tetraspanin/Peripherin.
DR InterPro; IPR000301; Tetraspanin_animals.
DR InterPro; IPR018503; Tetraspanin_CS.
DR InterPro; IPR008952; Tetraspanin_EC2_sf.
DR PANTHER; PTHR19282; PTHR19282; 1.
DR Pfam; PF00335; Tetraspanin; 1.
DR PIRSF; PIRSF002419; Tetraspanin; 1.
DR SUPFAM; SSF48652; SSF48652; 1.
DR PROSITE; PS00421; TM4_1; 1.
PE 1: Evidence at protein level;
KW Direct protein sequencing; Glycoprotein; Membrane; Reference proteome;
KW Transmembrane; Transmembrane helix.
FT CHAIN 1..221
FT /note="Tetraspanin-2"
FT /id="PRO_0000219237"
FT TOPO_DOM 1..13
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 14..34
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 35..54
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 55..75
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 76..90
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 91..111
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 112..188
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 189..209
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 210..221
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT CARBOHYD 139
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CONFLICT 68
FT /note="L -> M (in Ref. 1; BAB31109)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 221 AA; 24181 MW; 9DF8BE399DD3EE5A CRC64;
MGRFRGGLRC IKYLLLGFNL LFWLAGSAVI AFGLWFRFGG TMKDLSSEDK SPEYFYVGLY
VLVGAGALMM TVGFFGCCGA MRESQCVLGS FFTCLLVIFA AEVTTGVFAF IGKDVAIRHV
QSMYEEAYSD YLKDRARGNG TLITFHSAFQ CCGKESSEQV QPTCPKELPG HKNCIDKIET
VISAKLQLIG IVGIGIAGLT IFGMIFSMVL CCAIRNSRDV I
