TSN32_HUMAN
ID TSN32_HUMAN Reviewed; 320 AA.
AC Q96QS1; Q96KX4; Q9HC50; Q9HC51; Q9Y5U1;
DT 27-MAR-2002, integrated into UniProtKB/Swiss-Prot.
DT 01-DEC-2001, sequence version 1.
DT 03-AUG-2022, entry version 145.
DE RecName: Full=Tetraspanin-32;
DE Short=Tspan-32;
DE AltName: Full=Protein Phemx;
GN Name=TSPAN32; Synonyms=PHEMX, TSSC6;
OS Homo sapiens (Human).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae;
OC Homo.
OX NCBI_TaxID=9606;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2), AND TISSUE SPECIFICITY.
RX PubMed=10072438; DOI=10.1093/hmg/8.4.683;
RA Lee M.P., Brandenburg S., Landes G.M., Adams M., Miller G., Feinberg A.P.;
RT "Two novel genes in the center of the 11p15 imprinted domain escape genomic
RT imprinting.";
RL Hum. Mol. Genet. 8:683-690(1999).
RN [2]
RP NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
RX PubMed=11718897; DOI=10.1016/s0167-4781(01)00306-2;
RA Robb L., Tarrant J., Groom J., Ibrahim M., Li R., Borobakas B.,
RA Wright M.D.;
RT "Molecular characterisation of mouse and human TSSC6: evidence that TSSC6
RT is a genuine member of the tetraspanin superfamily and is expressed
RT specifically in haematopoietic organs.";
RL Biochim. Biophys. Acta 1522:31-41(2001).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 5).
RC TISSUE=Lung;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [4]
RP NUCLEOTIDE SEQUENCE [MRNA] OF 33-320 (ISOFORMS 3 AND 4), AND DEVELOPMENTAL
RP STAGE.
RX PubMed=10950922; DOI=10.1006/geno.2000.6277;
RA Nicholson R.H., Pantano S., Eliason J.F., Galy A., Weiler S., Kaplan J.,
RA Hughes M.R., Ko M.S.H.;
RT "Phemx, a novel mouse gene expressed in hematopoietic cells maps to the
RT imprinted cluster on distal chromosome 7.";
RL Genomics 68:13-21(2000).
RN [5]
RP SPLICE ISOFORM(S) THAT ARE POTENTIAL NMD TARGET(S).
RX PubMed=14759258; DOI=10.1186/gb-2004-5-2-r8;
RA Hillman R.T., Green R.E., Brenner S.E.;
RT "An unappreciated role for RNA surveillance.";
RL Genome Biol. 5:R8.1-R8.16(2004).
CC -!- SUBCELLULAR LOCATION: Membrane {ECO:0000305}; Multi-pass membrane
CC protein {ECO:0000305}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=5;
CC Comment=Additional isoforms seem to exist. Experimental confirmation
CC may be lacking for some isoforms.;
CC Name=1;
CC IsoId=Q96QS1-1; Sequence=Displayed;
CC Name=2;
CC IsoId=Q96QS1-2; Sequence=VSP_003932;
CC Name=3;
CC IsoId=Q96QS1-3; Sequence=VSP_003937, VSP_003938;
CC Name=4;
CC IsoId=Q96QS1-4; Sequence=VSP_003932, VSP_003933, VSP_003934;
CC Name=5;
CC IsoId=Q96QS1-5; Sequence=VSP_003935, VSP_003936;
CC -!- TISSUE SPECIFICITY: Expressed ubiquitously at low levels. High levels
CC of expression are confined to hematopoietic tissues including
CC peripheral blood leukocytes, thymus and spleen.
CC {ECO:0000269|PubMed:10072438}.
CC -!- DEVELOPMENTAL STAGE: Expressed from early embryogenesis through to
CC adulthood. {ECO:0000269|PubMed:10950922}.
CC -!- MISCELLANEOUS: [Isoform 4]: May be produced at very low levels due to a
CC premature stop codon in the mRNA, leading to nonsense-mediated mRNA
CC decay. {ECO:0000305}.
CC -!- MISCELLANEOUS: [Isoform 5]: May be produced at very low levels due to a
CC premature stop codon in the mRNA, leading to nonsense-mediated mRNA
CC decay. {ECO:0000305}.
CC -!- SIMILARITY: Belongs to the tetraspanin (TM4SF) family. {ECO:0000305}.
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DR EMBL; AF125569; AAD23580.1; -; mRNA.
DR EMBL; AY039001; AAK84431.1; -; mRNA.
DR EMBL; BC016693; AAH16693.1; -; mRNA.
DR EMBL; AF176070; AAG15840.1; -; mRNA.
DR EMBL; AF176071; AAG15841.1; -; mRNA.
DR CCDS; CCDS7733.1; -. [Q96QS1-1]
DR RefSeq; NP_620591.3; NM_139022.2. [Q96QS1-1]
DR AlphaFoldDB; Q96QS1; -.
DR STRING; 9606.ENSP00000182290; -.
DR iPTMnet; Q96QS1; -.
DR PhosphoSitePlus; Q96QS1; -.
DR BioMuta; TSPAN32; -.
DR DMDM; 20139069; -.
DR MassIVE; Q96QS1; -.
DR PaxDb; Q96QS1; -.
DR PeptideAtlas; Q96QS1; -.
DR PRIDE; Q96QS1; -.
DR ProteomicsDB; 77889; -. [Q96QS1-1]
DR ProteomicsDB; 77890; -. [Q96QS1-2]
DR ProteomicsDB; 77891; -. [Q96QS1-3]
DR ProteomicsDB; 77893; -. [Q96QS1-5]
DR Antibodypedia; 10387; 199 antibodies from 28 providers.
DR DNASU; 10077; -.
DR Ensembl; ENST00000182290.9; ENSP00000182290.5; ENSG00000064201.16. [Q96QS1-1]
DR Ensembl; ENST00000381121.7; ENSP00000370513.3; ENSG00000064201.16. [Q96QS1-3]
DR Ensembl; ENST00000446063.6; ENSP00000395018.2; ENSG00000064201.16. [Q96QS1-5]
DR GeneID; 10077; -.
DR KEGG; hsa:10077; -.
DR MANE-Select; ENST00000182290.9; ENSP00000182290.5; NM_139022.3; NP_620591.3.
DR UCSC; uc001lvy.2; human. [Q96QS1-1]
DR CTD; 10077; -.
DR DisGeNET; 10077; -.
DR GeneCards; TSPAN32; -.
DR HGNC; HGNC:13410; TSPAN32.
DR HPA; ENSG00000064201; Group enriched (bone marrow, heart muscle, lymphoid tissue).
DR MIM; 603853; gene.
DR neXtProt; NX_Q96QS1; -.
DR OpenTargets; ENSG00000064201; -.
DR PharmGKB; PA33257; -.
DR VEuPathDB; HostDB:ENSG00000064201; -.
DR eggNOG; ENOG502S0ED; Eukaryota.
DR GeneTree; ENSGT00390000003287; -.
DR HOGENOM; CLU_076116_0_0_1; -.
DR InParanoid; Q96QS1; -.
DR OMA; FTVYGMV; -.
DR OrthoDB; 1239506at2759; -.
DR PhylomeDB; Q96QS1; -.
DR TreeFam; TF336277; -.
DR PathwayCommons; Q96QS1; -.
DR BioGRID-ORCS; 10077; 9 hits in 1066 CRISPR screens.
DR ChiTaRS; TSPAN32; human.
DR GeneWiki; TSPAN32; -.
DR GenomeRNAi; 10077; -.
DR Pharos; Q96QS1; Tbio.
DR PRO; PR:Q96QS1; -.
DR Proteomes; UP000005640; Chromosome 11.
DR RNAct; Q96QS1; protein.
DR Bgee; ENSG00000064201; Expressed in granulocyte and 97 other tissues.
DR ExpressionAtlas; Q96QS1; baseline and differential.
DR Genevisible; Q96QS1; HS.
DR GO; GO:0009986; C:cell surface; IEA:Ensembl.
DR GO; GO:0005887; C:integral component of plasma membrane; IBA:GO_Central.
DR GO; GO:0070442; C:integrin alphaIIb-beta3 complex; IEA:Ensembl.
DR GO; GO:0007267; P:cell-cell signaling; TAS:UniProtKB.
DR GO; GO:0007010; P:cytoskeleton organization; IEA:Ensembl.
DR GO; GO:0042832; P:defense response to protozoan; IEA:Ensembl.
DR GO; GO:0007229; P:integrin-mediated signaling pathway; IEA:Ensembl.
DR GO; GO:0030886; P:negative regulation of myeloid dendritic cell activation; IEA:Ensembl.
DR GO; GO:0042130; P:negative regulation of T cell proliferation; IEA:Ensembl.
DR GO; GO:0070527; P:platelet aggregation; IEA:Ensembl.
DR GO; GO:0072659; P:protein localization to plasma membrane; IBA:GO_Central.
DR GO; GO:0051604; P:protein maturation; IBA:GO_Central.
DR GO; GO:0050688; P:regulation of defense response to virus; IEA:Ensembl.
DR GO; GO:0042098; P:T cell proliferation; IEA:Ensembl.
DR CDD; cd03153; PHEMX_like_LEL; 1.
DR Gene3D; 1.10.1450.10; -; 1.
DR InterPro; IPR042782; PHEMX_LEL.
DR InterPro; IPR018499; Tetraspanin/Peripherin.
DR InterPro; IPR008952; Tetraspanin_EC2_sf.
DR PANTHER; PTHR19282; PTHR19282; 1.
DR Pfam; PF00335; Tetraspanin; 1.
DR SUPFAM; SSF48652; SSF48652; 1.
PE 2: Evidence at transcript level;
KW Alternative splicing; Membrane; Reference proteome; Transmembrane;
KW Transmembrane helix.
FT CHAIN 1..320
FT /note="Tetraspanin-32"
FT /id="PRO_0000219278"
FT TRANSMEM 14..34
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 60..80
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 90..110
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 203..223
FT /note="Helical"
FT /evidence="ECO:0000255"
FT VAR_SEQ 1..30
FT /note="Missing (in isoform 2 and isoform 4)"
FT /evidence="ECO:0000303|PubMed:10072438,
FT ECO:0000303|PubMed:10950922"
FT /id="VSP_003932"
FT VAR_SEQ 137..154
FT /note="GTSHVRRQELAAIQDVFL -> VSVLWEEVSFQPSGEHRG (in isoform
FT 4)"
FT /evidence="ECO:0000303|PubMed:10950922"
FT /id="VSP_003933"
FT VAR_SEQ 155..320
FT /note="Missing (in isoform 4)"
FT /evidence="ECO:0000303|PubMed:10950922"
FT /id="VSP_003934"
FT VAR_SEQ 210..258
FT /note="VSALLFSSFLWFAIRCGCSLDRKGKYTLTPRACGRQPQEPSLLRCSQGG ->
FT LGPQGQIHPDPTSMWPPAPGAQPLEMLPGWTHTLSPLRSSCYWSKRMLG (in
FT isoform 5)"
FT /evidence="ECO:0000303|PubMed:15489334"
FT /id="VSP_003935"
FT VAR_SEQ 241..253
FT /note="ACGRQPQEPSLLR -> SPGQKSRWAQWVP (in isoform 3)"
FT /evidence="ECO:0000303|PubMed:10950922"
FT /id="VSP_003937"
FT VAR_SEQ 254..320
FT /note="Missing (in isoform 3)"
FT /evidence="ECO:0000303|PubMed:10950922"
FT /id="VSP_003938"
FT VAR_SEQ 259..320
FT /note="Missing (in isoform 5)"
FT /evidence="ECO:0000303|PubMed:15489334"
FT /id="VSP_003936"
SQ SEQUENCE 320 AA; 34631 MW; 534E4C29767B8E6D CRC64;
MGPWSRVRVA KCQMLVTCFF ILLLGLSVAT MVTLTYFGAH FAVIRRASLE KNPYQAVHQW
AFSAGLSLVG LLTLGAVLSA AATVREAQGL MAGGFLCFSL AFCAQVQVVF WRLHSPTQVE
DAMLDTYDLV YEQAMKGTSH VRRQELAAIQ DVFLCCGKKS PFSRLGSTEA DLCQGEEAAR
EDCLQGIRSF LRTHQQVASS LTSIGLALTV SALLFSSFLW FAIRCGCSLD RKGKYTLTPR
ACGRQPQEPS LLRCSQGGPT HCLHSEAVAI GPRGCSGSLR WLQESDAAPL PLSCHLAAHR
ALQGRSRGGL SGCPERGLSD
