TSN32_MOUSE
ID TSN32_MOUSE Reviewed; 256 AA.
AC Q9JHH2; Q923G9; Q9ESH0; Q9JHQ9; Q9JHR0; Q9JHR1; Q9JHR2; Q9JHS8;
DT 27-MAR-2002, integrated into UniProtKB/Swiss-Prot.
DT 27-MAR-2002, sequence version 2.
DT 03-AUG-2022, entry version 139.
DE RecName: Full=Tetraspanin-32;
DE Short=Tspan-32;
DE AltName: Full=AML1-regulated transmembrane protein 1;
DE AltName: Full=Protein Phemx;
GN Name=Tspan32; Synonyms=Art1, Phemx, Tssc6;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), TISSUE SPECIFICITY, AND
RP DEVELOPMENTAL STAGE.
RC STRAIN=C57BL/6J;
RX PubMed=10950922; DOI=10.1006/geno.2000.6277;
RA Nicholson R.H., Pantano S., Eliason J.F., Galy A., Weiler S., Kaplan J.,
RA Hughes M.R., Ko M.S.H.;
RT "Phemx, a novel mouse gene expressed in hematopoietic cells maps to the
RT imprinted cluster on distal chromosome 7.";
RL Genomics 68:13-21(2000).
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA] (ISOFORMS 1; 2; 3; 4 AND 5).
RC STRAIN=129/Sv, and C57BL/6J;
RX PubMed=10915772; DOI=10.1093/hmg/9.12.1829;
RA Paulsen M., El-Maarri O., Engemann S., Stroedicke M., Franck O., Davies K.,
RA Reinhardt R., Reik W., Walter J.;
RT "Sequence conservation and variability of imprinting in the Beckwith-
RT Wiedemann syndrome gene cluster in human and mouse.";
RL Hum. Mol. Genet. 9:1829-1841(2000).
RN [3]
RP NUCLEOTIDE SEQUENCE (ISOFORM 6).
RX PubMed=11718897; DOI=10.1016/s0167-4781(01)00306-2;
RA Robb L., Tarrant J., Groom J., Ibrahim M., Li R., Borobakas B.,
RA Wright M.D.;
RT "Molecular characterisation of mouse and human TSSC6: evidence that TSSC6
RT is a genuine member of the tetraspanin superfamily and is expressed
RT specifically in haematopoietic organs.";
RL Biochim. Biophys. Acta 1522:31-41(2001).
RN [4]
RP NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
RC STRAIN=C57BL/6J; TISSUE=Spleen;
RX PubMed=11396961; DOI=10.1006/bbrc.2001.5005;
RA Harada Y., Harada H., Downing J.R., Kimura A.;
RT "A hematopoietic-specific transmembrane protein, Art-1, is possibly
RT regulated by aml1.";
RL Biochem. Biophys. Res. Commun. 284:714-722(2001).
CC -!- SUBCELLULAR LOCATION: Membrane {ECO:0000305}; Multi-pass membrane
CC protein {ECO:0000305}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=6;
CC Comment=Additional isoforms seem to exist.;
CC Name=6;
CC IsoId=Q9JHH2-1; Sequence=Displayed;
CC Name=1;
CC IsoId=Q9JHH2-2; Sequence=VSP_003939;
CC Name=2;
CC IsoId=Q9JHH2-3; Sequence=VSP_003939, VSP_003941;
CC Name=3;
CC IsoId=Q9JHH2-4; Sequence=VSP_003939, VSP_003942, VSP_003943;
CC Name=4;
CC IsoId=Q9JHH2-5; Sequence=VSP_003939, VSP_003940, VSP_003941;
CC Name=5;
CC IsoId=Q9JHH2-6; Sequence=VSP_003939, VSP_003940, VSP_003942;
CC -!- TISSUE SPECIFICITY: Expressed exclusively in hematopoietic tissues.
CC Expression detected in spleen, thymus, bone marrow and peripheral blood
CC leukocytes but not in heart, brain, lung, liver, kidney or testis.
CC {ECO:0000269|PubMed:10950922}.
CC -!- DEVELOPMENTAL STAGE: Expressed from early embryogenesis through to
CC adulthood. {ECO:0000269|PubMed:10950922}.
CC -!- SIMILARITY: Belongs to the tetraspanin (TM4SF) family. {ECO:0000305}.
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; AF175771; AAG27268.1; -; mRNA.
DR EMBL; AJ251788; CAB94716.1; -; Genomic_DNA.
DR EMBL; AJ251835; CAB94777.1; -; Genomic_DNA.
DR EMBL; AJ279791; CAB94719.1; -; mRNA.
DR EMBL; AJ279792; CAB94720.1; -; mRNA.
DR EMBL; AJ279793; CAB94721.1; -; mRNA.
DR EMBL; AJ279794; CAB94722.1; -; mRNA.
DR EMBL; AJ279795; CAB94723.1; -; mRNA.
DR EMBL; AY039000; AAK84430.1; -; mRNA.
DR EMBL; AF291425; AAK83110.1; -; mRNA.
DR CCDS; CCDS52459.1; -. [Q9JHH2-1]
DR RefSeq; NP_001121552.1; NM_001128080.2. [Q9JHH2-1]
DR RefSeq; NP_001121553.1; NM_001128081.1.
DR RefSeq; NP_001121554.1; NM_001128082.1.
DR RefSeq; NP_064682.1; NM_020286.3.
DR AlphaFoldDB; Q9JHH2; -.
DR SMR; Q9JHH2; -.
DR STRING; 10090.ENSMUSP00000009396; -.
DR PhosphoSitePlus; Q9JHH2; -.
DR MaxQB; Q9JHH2; -.
DR PaxDb; Q9JHH2; -.
DR PRIDE; Q9JHH2; -.
DR Antibodypedia; 10387; 199 antibodies from 28 providers.
DR DNASU; 27027; -.
DR Ensembl; ENSMUST00000009396; ENSMUSP00000009396; ENSMUSG00000000244. [Q9JHH2-1]
DR GeneID; 27027; -.
DR KEGG; mmu:27027; -.
DR UCSC; uc009kom.2; mouse. [Q9JHH2-1]
DR CTD; 10077; -.
DR MGI; MGI:1350360; Tspan32.
DR VEuPathDB; HostDB:ENSMUSG00000000244; -.
DR eggNOG; ENOG502S0ED; Eukaryota.
DR GeneTree; ENSGT00390000003287; -.
DR HOGENOM; CLU_076116_0_0_1; -.
DR InParanoid; Q9JHH2; -.
DR OMA; FTVYGMV; -.
DR OrthoDB; 1239506at2759; -.
DR PhylomeDB; Q9JHH2; -.
DR TreeFam; TF336277; -.
DR BioGRID-ORCS; 27027; 2 hits in 71 CRISPR screens.
DR ChiTaRS; Art1; mouse.
DR PRO; PR:Q9JHH2; -.
DR Proteomes; UP000000589; Chromosome 7.
DR RNAct; Q9JHH2; protein.
DR Bgee; ENSMUSG00000000244; Expressed in granulocyte and 104 other tissues.
DR ExpressionAtlas; Q9JHH2; baseline and differential.
DR Genevisible; Q9JHH2; MM.
DR GO; GO:0009986; C:cell surface; IDA:MGI.
DR GO; GO:0005887; C:integral component of plasma membrane; IBA:GO_Central.
DR GO; GO:0070442; C:integrin alphaIIb-beta3 complex; IDA:MGI.
DR GO; GO:0007596; P:blood coagulation; IMP:MGI.
DR GO; GO:0007010; P:cytoskeleton organization; IMP:MGI.
DR GO; GO:0042832; P:defense response to protozoan; IGI:MGI.
DR GO; GO:0007599; P:hemostasis; IMP:MGI.
DR GO; GO:0007229; P:integrin-mediated signaling pathway; IMP:MGI.
DR GO; GO:0030886; P:negative regulation of myeloid dendritic cell activation; IGI:MGI.
DR GO; GO:0042130; P:negative regulation of T cell proliferation; IGI:MGI.
DR GO; GO:0070527; P:platelet aggregation; IMP:MGI.
DR GO; GO:0072659; P:protein localization to plasma membrane; IBA:GO_Central.
DR GO; GO:0051604; P:protein maturation; IBA:GO_Central.
DR GO; GO:0050688; P:regulation of defense response to virus; IGI:MGI.
DR GO; GO:0042098; P:T cell proliferation; IGI:MGI.
DR CDD; cd03153; PHEMX_like_LEL; 1.
DR Gene3D; 1.10.1450.10; -; 1.
DR InterPro; IPR042782; PHEMX_LEL.
DR InterPro; IPR018499; Tetraspanin/Peripherin.
DR InterPro; IPR000301; Tetraspanin_animals.
DR InterPro; IPR008952; Tetraspanin_EC2_sf.
DR PANTHER; PTHR19282; PTHR19282; 1.
DR PIRSF; PIRSF002419; Tetraspanin; 1.
DR SUPFAM; SSF48652; SSF48652; 1.
PE 2: Evidence at transcript level;
KW Alternative splicing; Membrane; Reference proteome; Transmembrane;
KW Transmembrane helix.
FT CHAIN 1..256
FT /note="Tetraspanin-32"
FT /id="PRO_0000219279"
FT TRANSMEM 15..35
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 61..81
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 90..110
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 203..223
FT /note="Helical"
FT /evidence="ECO:0000255"
FT VAR_SEQ 1..30
FT /note="Missing (in isoform 1, isoform 2, isoform 3, isoform
FT 4 and isoform 5)"
FT /evidence="ECO:0000303|PubMed:10915772,
FT ECO:0000303|PubMed:10950922, ECO:0000303|PubMed:11396961"
FT /id="VSP_003939"
FT VAR_SEQ 119..152
FT /note="Missing (in isoform 4 and isoform 5)"
FT /evidence="ECO:0000303|PubMed:10915772"
FT /id="VSP_003940"
FT VAR_SEQ 182..209
FT /note="Missing (in isoform 2 and isoform 4)"
FT /evidence="ECO:0000303|PubMed:10915772"
FT /id="VSP_003941"
FT VAR_SEQ 182..206
FT /note="DCLQSIRNVLWTHYSIASILTCTSL -> IPWFPDPGTQPLQMDLGWAGASM
FT PC (in isoform 3 and isoform 5)"
FT /evidence="ECO:0000303|PubMed:10915772"
FT /id="VSP_003942"
FT VAR_SEQ 207..236
FT /note="Missing (in isoform 3)"
FT /evidence="ECO:0000303|PubMed:10915772"
FT /id="VSP_003943"
FT CONFLICT 174
FT /note="Q -> L (in Ref. 1; AAG27268)"
FT /evidence="ECO:0000305"
FT CONFLICT 235
FT /note="Y -> F (in Ref. 1; AAG27268)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 256 AA; 28906 MW; A20EF0F6248FF67A CRC64;
MGHWNRIKIA KCQILITNFL VLLLGLSMAT MVVVIHFGDH FTVIGHASLE RNPYETLRYW
AFYVGISLAG LLSLGAALST IATVREAHGL MAAGFLCFAL SFCILVQVAF WRFYNPTQVE
DAVLDTYDFV YDQAMKSPSS NWWQELAVIQ DTFLCCGKKS PFGLLVSTGA IMCQGREAMR
EDCLQSIRNV LWTHYSIASI LTCTSLALTV YAMMLCAFLW FAIHSYHGLD RKGRYSLTPP
RSHGFQTQEP SLFRWT
