TSN3_HUMAN
ID TSN3_HUMAN Reviewed; 253 AA.
AC O60637; A6NEH4; B3KQQ2; B4DP19; Q9BW22; Q9NVX9;
DT 01-DEC-2000, integrated into UniProtKB/Swiss-Prot.
DT 01-AUG-1998, sequence version 1.
DT 03-AUG-2022, entry version 177.
DE RecName: Full=Tetraspanin-3;
DE Short=Tspan-3;
DE AltName: Full=Tetraspanin TM4-A;
DE AltName: Full=Transmembrane 4 superfamily member 8;
GN Name=TSPAN3; Synonyms=TM4SF8;
OS Homo sapiens (Human).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae;
OC Homo.
OX NCBI_TaxID=9606;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
RX PubMed=9714763; DOI=10.1016/s0167-4781(98)00087-6;
RA Todd S.C., Doctor V.S., Levy S.;
RT "Sequences and expression of six new members of the tetraspanin/TM4SF
RT family.";
RL Biochim. Biophys. Acta 1399:101-104(1998).
RN [2]
RP NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
RA Puls K.L., Ni J., Liu D., Morahan G., Wright M.D.;
RT "The molecular characterization of four tetraspanins.";
RL Submitted (MAR-1999) to the EMBL/GenBank/DDBJ databases.
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 3).
RX PubMed=14702039; DOI=10.1038/ng1285;
RA Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R.,
RA Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H.,
RA Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.,
RA Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K.,
RA Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H.,
RA Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M.,
RA Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K.,
RA Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T.,
RA Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M.,
RA Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S.,
RA Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H.,
RA Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K.,
RA Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N.,
RA Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S.,
RA Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O.,
RA Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H.,
RA Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B.,
RA Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y.,
RA Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K.,
RA Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T.,
RA Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T.,
RA Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y.,
RA Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H.,
RA Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y.,
RA Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H.,
RA Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O.,
RA Isogai T., Sugano S.;
RT "Complete sequencing and characterization of 21,243 full-length human
RT cDNAs.";
RL Nat. Genet. 36:40-45(2004).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
RC TISSUE=Ovary;
RX PubMed=16303743; DOI=10.1093/dnares/12.2.117;
RA Otsuki T., Ota T., Nishikawa T., Hayashi K., Suzuki Y., Yamamoto J.,
RA Wakamatsu A., Kimura K., Sakamoto K., Hatano N., Kawai Y., Ishii S.,
RA Saito K., Kojima S., Sugiyama T., Ono T., Okano K., Yoshikawa Y.,
RA Aotsuka S., Sasaki N., Hattori A., Okumura K., Nagai K., Sugano S.,
RA Isogai T.;
RT "Signal sequence and keyword trap in silico for selection of full-length
RT human cDNAs encoding secretion or membrane proteins from oligo-capped cDNA
RT libraries.";
RL DNA Res. 12:117-126(2005).
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX PubMed=16572171; DOI=10.1038/nature04601;
RA Zody M.C., Garber M., Sharpe T., Young S.K., Rowen L., O'Neill K.,
RA Whittaker C.A., Kamal M., Chang J.L., Cuomo C.A., Dewar K.,
RA FitzGerald M.G., Kodira C.D., Madan A., Qin S., Yang X., Abbasi N.,
RA Abouelleil A., Arachchi H.M., Baradarani L., Birditt B., Bloom S.,
RA Bloom T., Borowsky M.L., Burke J., Butler J., Cook A., DeArellano K.,
RA DeCaprio D., Dorris L. III, Dors M., Eichler E.E., Engels R., Fahey J.,
RA Fleetwood P., Friedman C., Gearin G., Hall J.L., Hensley G., Johnson E.,
RA Jones C., Kamat A., Kaur A., Locke D.P., Madan A., Munson G., Jaffe D.B.,
RA Lui A., Macdonald P., Mauceli E., Naylor J.W., Nesbitt R., Nicol R.,
RA O'Leary S.B., Ratcliffe A., Rounsley S., She X., Sneddon K.M.B.,
RA Stewart S., Sougnez C., Stone S.M., Topham K., Vincent D., Wang S.,
RA Zimmer A.R., Birren B.W., Hood L., Lander E.S., Nusbaum C.;
RT "Analysis of the DNA sequence and duplication history of human chromosome
RT 15.";
RL Nature 440:671-675(2006).
RN [6]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RA Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M.,
RA Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J.,
RA Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S.,
RA Turner R., Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H.,
RA Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K.,
RA Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D.,
RA Hunkapiller M.W., Myers E.W., Venter J.C.;
RL Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases.
RN [7]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
RC TISSUE=Cervix, Colon, Muscle, and Skin;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
CC -!- FUNCTION: Regulates the proliferation and migration of
CC oligodendrocytes, a process essential for normal myelination and
CC repair. {ECO:0000250}.
CC -!- SUBUNIT: Interacts with claudin-11/CLDN11 and integrins. {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Membrane {ECO:0000305}; Multi-pass membrane
CC protein {ECO:0000305}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=3;
CC Name=1;
CC IsoId=O60637-1; Sequence=Displayed;
CC Name=2;
CC IsoId=O60637-2; Sequence=VSP_042679;
CC Name=3;
CC IsoId=O60637-3; Sequence=VSP_043519;
CC -!- SIMILARITY: Belongs to the tetraspanin (TM4SF) family. {ECO:0000305}.
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DR EMBL; AF054840; AAC69716.1; -; mRNA.
DR EMBL; AF133423; AAF08362.1; -; mRNA.
DR EMBL; AK001326; BAA91627.1; -; mRNA.
DR EMBL; AK001305; BAA91613.1; -; mRNA.
DR EMBL; AK001310; BAA91615.1; -; mRNA.
DR EMBL; AK075336; BAG52114.1; -; mRNA.
DR EMBL; AK075482; BAG52152.1; -; mRNA.
DR EMBL; AK298157; BAG60431.1; -; mRNA.
DR EMBL; AC090181; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; CH471136; EAW99209.1; -; Genomic_DNA.
DR EMBL; CH471136; EAW99210.1; -; Genomic_DNA.
DR EMBL; CH471136; EAW99211.1; -; Genomic_DNA.
DR EMBL; BC000704; AAH00704.1; -; mRNA.
DR EMBL; BC004280; AAH04280.1; -; mRNA.
DR EMBL; BC009248; AAH09248.1; -; mRNA.
DR EMBL; BC011206; AAH11206.1; -; mRNA.
DR CCDS; CCDS10292.1; -. [O60637-1]
DR CCDS; CCDS10293.1; -. [O60637-2]
DR CCDS; CCDS53963.1; -. [O60637-3]
DR PIR; A59264; A59264.
DR RefSeq; NP_001161884.1; NM_001168412.1. [O60637-3]
DR RefSeq; NP_005715.1; NM_005724.5. [O60637-1]
DR RefSeq; NP_944492.1; NM_198902.2. [O60637-2]
DR AlphaFoldDB; O60637; -.
DR SMR; O60637; -.
DR BioGRID; 115406; 100.
DR IntAct; O60637; 50.
DR MINT; O60637; -.
DR STRING; 9606.ENSP00000267970; -.
DR TCDB; 8.A.40.1.16; the tetraspanin (tetraspanin) family.
DR GlyConnect; 710; 20 N-Linked glycans (2 sites).
DR GlyGen; O60637; 7 sites, 21 N-linked glycans (2 sites), 1 O-linked glycan (1 site).
DR iPTMnet; O60637; -.
DR PhosphoSitePlus; O60637; -.
DR SwissPalm; O60637; -.
DR BioMuta; TSPAN3; -.
DR jPOST; O60637; -.
DR MassIVE; O60637; -.
DR MaxQB; O60637; -.
DR PaxDb; O60637; -.
DR PeptideAtlas; O60637; -.
DR PRIDE; O60637; -.
DR ProteomicsDB; 49490; -. [O60637-1]
DR ProteomicsDB; 49491; -. [O60637-2]
DR ProteomicsDB; 49492; -. [O60637-3]
DR Antibodypedia; 3085; 163 antibodies from 20 providers.
DR DNASU; 10099; -.
DR Ensembl; ENST00000267970.9; ENSP00000267970.4; ENSG00000140391.15. [O60637-1]
DR Ensembl; ENST00000346495.6; ENSP00000341329.2; ENSG00000140391.15. [O60637-2]
DR Ensembl; ENST00000424443.7; ENSP00000407243.3; ENSG00000140391.15. [O60637-3]
DR GeneID; 10099; -.
DR KEGG; hsa:10099; -.
DR MANE-Select; ENST00000267970.9; ENSP00000267970.4; NM_005724.6; NP_005715.1.
DR UCSC; uc002bcj.4; human. [O60637-1]
DR CTD; 10099; -.
DR DisGeNET; 10099; -.
DR GeneCards; TSPAN3; -.
DR HGNC; HGNC:17752; TSPAN3.
DR HPA; ENSG00000140391; Low tissue specificity.
DR MIM; 613134; gene.
DR neXtProt; NX_O60637; -.
DR OpenTargets; ENSG00000140391; -.
DR PharmGKB; PA134913500; -.
DR VEuPathDB; HostDB:ENSG00000140391; -.
DR eggNOG; KOG3882; Eukaryota.
DR GeneTree; ENSGT00940000154954; -.
DR HOGENOM; CLU_055524_5_2_1; -.
DR InParanoid; O60637; -.
DR OMA; CRETARS; -.
DR OrthoDB; 1406905at2759; -.
DR PhylomeDB; O60637; -.
DR TreeFam; TF316345; -.
DR PathwayCommons; O60637; -.
DR SignaLink; O60637; -.
DR BioGRID-ORCS; 10099; 11 hits in 1088 CRISPR screens.
DR ChiTaRS; TSPAN3; human.
DR GeneWiki; TSPAN3; -.
DR GenomeRNAi; 10099; -.
DR Pharos; O60637; Tbio.
DR PRO; PR:O60637; -.
DR Proteomes; UP000005640; Chromosome 15.
DR RNAct; O60637; protein.
DR Bgee; ENSG00000140391; Expressed in bronchial epithelial cell and 200 other tissues.
DR ExpressionAtlas; O60637; baseline and differential.
DR Genevisible; O60637; HS.
DR GO; GO:0070062; C:extracellular exosome; HDA:UniProtKB.
DR GO; GO:0005887; C:integral component of plasma membrane; IBA:GO_Central.
DR Gene3D; 1.10.1450.10; -; 1.
DR InterPro; IPR018499; Tetraspanin/Peripherin.
DR InterPro; IPR000301; Tetraspanin_animals.
DR InterPro; IPR018503; Tetraspanin_CS.
DR InterPro; IPR008952; Tetraspanin_EC2_sf.
DR PANTHER; PTHR19282; PTHR19282; 1.
DR Pfam; PF00335; Tetraspanin; 1.
DR PIRSF; PIRSF002419; Tetraspanin; 1.
DR SUPFAM; SSF48652; SSF48652; 1.
DR PROSITE; PS00421; TM4_1; 1.
PE 2: Evidence at transcript level;
KW Alternative splicing; Glycoprotein; Membrane; Reference proteome;
KW Transmembrane; Transmembrane helix.
FT CHAIN 1..253
FT /note="Tetraspanin-3"
FT /id="PRO_0000219239"
FT TOPO_DOM 1..11
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 12..32
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 33..50
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 51..71
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 72..85
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 86..106
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 107..212
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 213..233
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 234..253
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT CARBOHYD 127
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 152
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 167
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 183
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT VAR_SEQ 22..85
FT /note="Missing (in isoform 3)"
FT /evidence="ECO:0000303|PubMed:14702039"
FT /id="VSP_043519"
FT VAR_SEQ 86..110
FT /note="Missing (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:16303743"
FT /id="VSP_042679"
FT CONFLICT 85
FT /note="Missing (in Ref. 7; AAH00704)"
FT /evidence="ECO:0000305"
FT CONFLICT 236
FT /note="R -> G (in Ref. 3; BAA91613)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 253 AA; 28018 MW; E074A4CD57229EC6 CRC64;
MGQCGITSSK TVLVFLNLIF WGAAGILCYV GAYVFITYDD YDHFFEDVYT LIPAVVIIAV
GALLFIIGLI GCCATIRESR CGLATFVIIL LLVFVTEVVV VVLGYVYRAK VENEVDRSIQ
KVYKTYNGTN PDAASRAIDY VQRQLHCCGI HNYSDWENTD WFKETKNQSV PLSCCRETAS
NCNGSLAHPS DLYAEGCEAL VVKKLQEIMM HVIWAALAFA AIQLLGMLCA CIVLCRRSRD
PAYELLITGG TYA
