TSN8_HUMAN
ID TSN8_HUMAN Reviewed; 237 AA.
AC P19075; B2R7T7; Q9BS78;
DT 01-NOV-1990, integrated into UniProtKB/Swiss-Prot.
DT 01-NOV-1990, sequence version 1.
DT 03-AUG-2022, entry version 177.
DE RecName: Full=Tetraspanin-8;
DE Short=Tspan-8;
DE AltName: Full=Transmembrane 4 superfamily member 3;
DE AltName: Full=Tumor-associated antigen CO-029;
GN Name=TSPAN8; Synonyms=TM4SF3;
OS Homo sapiens (Human).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae;
OC Homo.
OX NCBI_TaxID=9606;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA].
RC TISSUE=Colon carcinoma;
RX PubMed=2395876; DOI=10.1073/pnas.87.17.6833;
RA Szala S., Kasai Y., Steplewski Z., Rodeck U., Koprowski H.,
RA Linnenbach A.J.;
RT "Molecular cloning of cDNA for the human tumor-associated antigen CO-029
RT and identification of related transmembrane antigens.";
RL Proc. Natl. Acad. Sci. U.S.A. 87:6833-6837(1990).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC TISSUE=Colon;
RX PubMed=14702039; DOI=10.1038/ng1285;
RA Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R.,
RA Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H.,
RA Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.,
RA Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K.,
RA Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H.,
RA Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M.,
RA Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K.,
RA Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T.,
RA Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M.,
RA Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S.,
RA Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H.,
RA Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K.,
RA Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N.,
RA Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S.,
RA Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O.,
RA Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H.,
RA Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B.,
RA Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y.,
RA Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K.,
RA Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T.,
RA Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T.,
RA Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y.,
RA Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H.,
RA Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y.,
RA Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H.,
RA Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O.,
RA Isogai T., Sugano S.;
RT "Complete sequencing and characterization of 21,243 full-length human
RT cDNAs.";
RL Nat. Genet. 36:40-45(2004).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RA Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M.,
RA Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J.,
RA Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S.,
RA Turner R., Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H.,
RA Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K.,
RA Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D.,
RA Hunkapiller M.W., Myers E.W., Venter J.C.;
RL Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases.
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA], AND VARIANTS ILE-35 AND ALA-73.
RC TISSUE=Liver;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [5]
RP GLYCOSYLATION [LARGE SCALE ANALYSIS] AT ASN-118.
RC TISSUE=Liver;
RX PubMed=19159218; DOI=10.1021/pr8008012;
RA Chen R., Jiang X., Sun D., Han G., Wang F., Ye M., Wang L., Zou H.;
RT "Glycoproteomics analysis of human liver tissue by combination of multiple
RT enzyme digestion and hydrazide chemistry.";
RL J. Proteome Res. 8:651-661(2009).
CC -!- INTERACTION:
CC P19075; Q16820: MEP1B; NbExp=4; IntAct=EBI-4289938, EBI-968418;
CC P19075; P54315: PNLIPRP1; NbExp=3; IntAct=EBI-4289938, EBI-8652812;
CC P19075; Q9NVC3: SLC38A7; NbExp=3; IntAct=EBI-4289938, EBI-10314552;
CC P19075; P32856-2: STX2; NbExp=3; IntAct=EBI-4289938, EBI-11956649;
CC P19075; O14925: TIMM23; NbExp=3; IntAct=EBI-4289938, EBI-1047996;
CC -!- SUBCELLULAR LOCATION: Membrane; Multi-pass membrane protein.
CC -!- TISSUE SPECIFICITY: Gastric, colon, rectal, and pancreatic carcinomas.
CC -!- SIMILARITY: Belongs to the tetraspanin (TM4SF) family. {ECO:0000305}.
CC -!- WEB RESOURCE: Name=Atlas of Genetics and Cytogenetics in Oncology and
CC Haematology;
CC URL="http://atlasgeneticsoncology.org/Genes/TSPAN8ID42585ch12q21.html";
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DR EMBL; M35252; AAA35709.1; -; mRNA.
DR EMBL; AK313112; BAG35934.1; -; mRNA.
DR EMBL; CH471054; EAW97257.1; -; Genomic_DNA.
DR EMBL; BC005246; AAH05246.1; -; mRNA.
DR CCDS; CCDS8999.1; -.
DR PIR; A36056; A36056.
DR RefSeq; NP_004607.1; NM_004616.2.
DR RefSeq; XP_006719646.1; XM_006719583.3.
DR RefSeq; XP_016875402.1; XM_017019913.1.
DR AlphaFoldDB; P19075; -.
DR SMR; P19075; -.
DR BioGRID; 112958; 79.
DR IntAct; P19075; 6.
DR STRING; 9606.ENSP00000377003; -.
DR GlyGen; P19075; 1 site.
DR iPTMnet; P19075; -.
DR PhosphoSitePlus; P19075; -.
DR BioMuta; TSPAN8; -.
DR DMDM; 116589; -.
DR jPOST; P19075; -.
DR MassIVE; P19075; -.
DR MaxQB; P19075; -.
DR PaxDb; P19075; -.
DR PeptideAtlas; P19075; -.
DR PRIDE; P19075; -.
DR ProteomicsDB; 53629; -.
DR Antibodypedia; 29509; 314 antibodies from 37 providers.
DR DNASU; 7103; -.
DR Ensembl; ENST00000247829.8; ENSP00000247829.3; ENSG00000127324.9.
DR Ensembl; ENST00000393330.6; ENSP00000377003.2; ENSG00000127324.9.
DR Ensembl; ENST00000546561.2; ENSP00000447160.1; ENSG00000127324.9.
DR GeneID; 7103; -.
DR KEGG; hsa:7103; -.
DR MANE-Select; ENST00000247829.8; ENSP00000247829.3; NM_004616.3; NP_004607.1.
DR UCSC; uc001swj.2; human.
DR CTD; 7103; -.
DR DisGeNET; 7103; -.
DR GeneCards; TSPAN8; -.
DR HGNC; HGNC:11855; TSPAN8.
DR HPA; ENSG00000127324; Tissue enhanced (intestine, stomach).
DR MIM; 600769; gene.
DR neXtProt; NX_P19075; -.
DR OpenTargets; ENSG00000127324; -.
DR PharmGKB; PA36556; -.
DR VEuPathDB; HostDB:ENSG00000127324; -.
DR eggNOG; KOG3882; Eukaryota.
DR GeneTree; ENSGT00940000158153; -.
DR HOGENOM; CLU_055524_4_2_1; -.
DR InParanoid; P19075; -.
DR OMA; GCLKYSM; -.
DR OrthoDB; 1205716at2759; -.
DR PhylomeDB; P19075; -.
DR TreeFam; TF352895; -.
DR PathwayCommons; P19075; -.
DR SignaLink; P19075; -.
DR BioGRID-ORCS; 7103; 6 hits in 1004 CRISPR screens.
DR ChiTaRS; TSPAN8; human.
DR GeneWiki; TSPAN8; -.
DR GenomeRNAi; 7103; -.
DR Pharos; P19075; Tbio.
DR PRO; PR:P19075; -.
DR Proteomes; UP000005640; Chromosome 12.
DR RNAct; P19075; protein.
DR Bgee; ENSG00000127324; Expressed in colonic mucosa and 176 other tissues.
DR Genevisible; P19075; HS.
DR GO; GO:0009986; C:cell surface; IEA:Ensembl.
DR GO; GO:0070062; C:extracellular exosome; HDA:UniProtKB.
DR GO; GO:0005887; C:integral component of plasma membrane; IBA:GO_Central.
DR GO; GO:0005178; F:integrin binding; IEA:Ensembl.
DR GO; GO:0030195; P:negative regulation of blood coagulation; IEA:Ensembl.
DR GO; GO:0010468; P:regulation of gene expression; IEA:Ensembl.
DR GO; GO:0007283; P:spermatogenesis; IEA:Ensembl.
DR Gene3D; 1.10.1450.10; -; 1.
DR InterPro; IPR018499; Tetraspanin/Peripherin.
DR InterPro; IPR000301; Tetraspanin_animals.
DR InterPro; IPR018503; Tetraspanin_CS.
DR InterPro; IPR008952; Tetraspanin_EC2_sf.
DR PANTHER; PTHR19282; PTHR19282; 1.
DR Pfam; PF00335; Tetraspanin; 1.
DR PIRSF; PIRSF002419; Tetraspanin; 1.
DR SUPFAM; SSF48652; SSF48652; 1.
DR PROSITE; PS00421; TM4_1; 1.
PE 1: Evidence at protein level;
KW Glycoprotein; Membrane; Reference proteome; Transmembrane;
KW Transmembrane helix.
FT CHAIN 1..237
FT /note="Tetraspanin-8"
FT /id="PRO_0000219252"
FT TOPO_DOM 1..9
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 10..33
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 34..57
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 58..72
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 73..83
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 84..109
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 110..205
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 206..230
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 231..237
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT CARBOHYD 118
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000269|PubMed:19159218"
FT VARIANT 35
FT /note="V -> I (in dbSNP:rs17849952)"
FT /evidence="ECO:0000269|PubMed:15489334"
FT /id="VAR_061848"
FT VARIANT 73
FT /note="G -> A (in dbSNP:rs3763978)"
FT /evidence="ECO:0000269|PubMed:15489334"
FT /id="VAR_020093"
FT VARIANT 213
FT /note="S -> A (in dbSNP:rs1051334)"
FT /id="VAR_012054"
SQ SEQUENCE 237 AA; 26044 MW; D1058F054EE8C14E CRC64;
MAGVSACIKY SMFTFNFLFW LCGILILALA IWVRVSNDSQ AIFGSEDVGS SSYVAVDILI
AVGAIIMILG FLGCCGAIKE SRCMLLLFFI GLLLILLLQV ATGILGAVFK SKSDRIVNET
LYENTKLLSA TGESEKQFQE AIIVFQEEFK CCGLVNGAAD WGNNFQHYPE LCACLDKQRP
CQSYNGKQVY KETCISFIKD FLAKNLIIVI GISFGLAVIE ILGLVFSMVL YCQIGNK
