TSN9_HUMAN
ID TSN9_HUMAN Reviewed; 239 AA.
AC O75954; D3DUQ7; Q53FV2; Q6FGJ8;
DT 01-DEC-2000, integrated into UniProtKB/Swiss-Prot.
DT 01-NOV-1998, sequence version 1.
DT 03-AUG-2022, entry version 152.
DE RecName: Full=Tetraspanin-9;
DE Short=Tspan-9;
DE AltName: Full=Tetraspan NET-5;
GN Name=TSPAN9; Synonyms=NET5;
OS Homo sapiens (Human).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae;
OC Homo.
OX NCBI_TaxID=9606;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA].
RX PubMed=10719184; DOI=10.1016/s0167-4838(00)00022-4;
RA Serru V., Dessen P., Boucheix C., Rubinstein E.;
RT "Sequence and expression of seven new tetraspans.";
RL Biochim. Biophys. Acta 1478:159-163(2000).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC TISSUE=Embryo;
RX PubMed=14702039; DOI=10.1038/ng1285;
RA Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R.,
RA Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H.,
RA Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.,
RA Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K.,
RA Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H.,
RA Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M.,
RA Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K.,
RA Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T.,
RA Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M.,
RA Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S.,
RA Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H.,
RA Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K.,
RA Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N.,
RA Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S.,
RA Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O.,
RA Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H.,
RA Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B.,
RA Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y.,
RA Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K.,
RA Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T.,
RA Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T.,
RA Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y.,
RA Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H.,
RA Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y.,
RA Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H.,
RA Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O.,
RA Isogai T., Sugano S.;
RT "Complete sequencing and characterization of 21,243 full-length human
RT cDNAs.";
RL Nat. Genet. 36:40-45(2004).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RA Ebert L., Schick M., Neubert P., Schatten R., Henze S., Korn B.;
RT "Cloning of human full open reading frames in Gateway(TM) system entry
RT vector (pDONR201).";
RL Submitted (JUN-2004) to the EMBL/GenBank/DDBJ databases.
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC TISSUE=Pancreas;
RA Suzuki Y., Sugano S., Totoki Y., Toyoda A., Takeda T., Sakaki Y.,
RA Tanaka A., Yokoyama S.;
RL Submitted (APR-2005) to the EMBL/GenBank/DDBJ databases.
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX PubMed=16541075; DOI=10.1038/nature04569;
RA Scherer S.E., Muzny D.M., Buhay C.J., Chen R., Cree A., Ding Y.,
RA Dugan-Rocha S., Gill R., Gunaratne P., Harris R.A., Hawes A.C.,
RA Hernandez J., Hodgson A.V., Hume J., Jackson A., Khan Z.M., Kovar-Smith C.,
RA Lewis L.R., Lozado R.J., Metzker M.L., Milosavljevic A., Miner G.R.,
RA Montgomery K.T., Morgan M.B., Nazareth L.V., Scott G., Sodergren E.,
RA Song X.-Z., Steffen D., Lovering R.C., Wheeler D.A., Worley K.C., Yuan Y.,
RA Zhang Z., Adams C.Q., Ansari-Lari M.A., Ayele M., Brown M.J., Chen G.,
RA Chen Z., Clerc-Blankenburg K.P., Davis C., Delgado O., Dinh H.H.,
RA Draper H., Gonzalez-Garay M.L., Havlak P., Jackson L.R., Jacob L.S.,
RA Kelly S.H., Li L., Li Z., Liu J., Liu W., Lu J., Maheshwari M.,
RA Nguyen B.-V., Okwuonu G.O., Pasternak S., Perez L.M., Plopper F.J.H.,
RA Santibanez J., Shen H., Tabor P.E., Verduzco D., Waldron L., Wang Q.,
RA Williams G.A., Zhang J., Zhou J., Allen C.C., Amin A.G., Anyalebechi V.,
RA Bailey M., Barbaria J.A., Bimage K.E., Bryant N.P., Burch P.E.,
RA Burkett C.E., Burrell K.L., Calderon E., Cardenas V., Carter K., Casias K.,
RA Cavazos I., Cavazos S.R., Ceasar H., Chacko J., Chan S.N., Chavez D.,
RA Christopoulos C., Chu J., Cockrell R., Cox C.D., Dang M., Dathorne S.R.,
RA David R., Davis C.M., Davy-Carroll L., Deshazo D.R., Donlin J.E.,
RA D'Souza L., Eaves K.A., Egan A., Emery-Cohen A.J., Escotto M., Flagg N.,
RA Forbes L.D., Gabisi A.M., Garza M., Hamilton C., Henderson N.,
RA Hernandez O., Hines S., Hogues M.E., Huang M., Idlebird D.G., Johnson R.,
RA Jolivet A., Jones S., Kagan R., King L.M., Leal B., Lebow H., Lee S.,
RA LeVan J.M., Lewis L.C., London P., Lorensuhewa L.M., Loulseged H.,
RA Lovett D.A., Lucier A., Lucier R.L., Ma J., Madu R.C., Mapua P.,
RA Martindale A.D., Martinez E., Massey E., Mawhiney S., Meador M.G.,
RA Mendez S., Mercado C., Mercado I.C., Merritt C.E., Miner Z.L., Minja E.,
RA Mitchell T., Mohabbat F., Mohabbat K., Montgomery B., Moore N., Morris S.,
RA Munidasa M., Ngo R.N., Nguyen N.B., Nickerson E., Nwaokelemeh O.O.,
RA Nwokenkwo S., Obregon M., Oguh M., Oragunye N., Oviedo R.J., Parish B.J.,
RA Parker D.N., Parrish J., Parks K.L., Paul H.A., Payton B.A., Perez A.,
RA Perrin W., Pickens A., Primus E.L., Pu L.-L., Puazo M., Quiles M.M.,
RA Quiroz J.B., Rabata D., Reeves K., Ruiz S.J., Shao H., Sisson I.,
RA Sonaike T., Sorelle R.P., Sutton A.E., Svatek A.F., Svetz L.A.,
RA Tamerisa K.S., Taylor T.R., Teague B., Thomas N., Thorn R.D., Trejos Z.Y.,
RA Trevino B.K., Ukegbu O.N., Urban J.B., Vasquez L.I., Vera V.A.,
RA Villasana D.M., Wang L., Ward-Moore S., Warren J.T., Wei X., White F.,
RA Williamson A.L., Wleczyk R., Wooden H.S., Wooden S.H., Yen J., Yoon L.,
RA Yoon V., Zorrilla S.E., Nelson D., Kucherlapati R., Weinstock G.,
RA Gibbs R.A.;
RT "The finished DNA sequence of human chromosome 12.";
RL Nature 440:346-351(2006).
RN [6]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RA Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M.,
RA Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J.,
RA Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S.,
RA Turner R., Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H.,
RA Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K.,
RA Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D.,
RA Hunkapiller M.W., Myers E.W., Venter J.C.;
RL Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases.
RN [7]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC TISSUE=Salivary gland;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [8]
RP TISSUE SPECIFICITY.
RX PubMed=18795891; DOI=10.1042/bj20081126;
RA Protty M.B., Watkins N.A., Colombo D., Thomas S.G., Heath V.L.,
RA Herbert J.M., Bicknell R., Senis Y.A., Ashman L.K., Berditchevski F.,
RA Ouwehand W.H., Watson S.P., Tomlinson M.G.;
RT "Identification of Tspan9 as a novel platelet tetraspanin and the collagen
RT receptor GPVI as a component of tetraspanin microdomains.";
RL Biochem. J. 417:391-400(2009).
CC -!- SUBUNIT: Found in a complex with GP6. {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Membrane {ECO:0000250}; Multi-pass membrane
CC protein {ECO:0000250}. Note=Colocalizes with GP6 in tetraspanin
CC microdomains on the platelet surface. {ECO:0000250}.
CC -!- TISSUE SPECIFICITY: Expressed in megakaryocytes and platelets (at
CC protein level). {ECO:0000269|PubMed:18795891}.
CC -!- PTM: Glycosylated. {ECO:0000250}.
CC -!- SIMILARITY: Belongs to the tetraspanin (TM4SF) family. {ECO:0000305}.
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DR EMBL; AF089749; AAC35859.1; -; mRNA.
DR EMBL; AK027366; BAG51307.1; -; mRNA.
DR EMBL; CR542109; CAG46906.1; -; mRNA.
DR EMBL; AK223179; BAD96899.1; -; mRNA.
DR EMBL; AC005912; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; CH471116; EAW88868.1; -; Genomic_DNA.
DR EMBL; CH471116; EAW88869.1; -; Genomic_DNA.
DR EMBL; BC071881; AAH71881.1; -; mRNA.
DR CCDS; CCDS8520.1; -.
DR RefSeq; NP_001161792.1; NM_001168320.1.
DR RefSeq; NP_006666.1; NM_006675.4.
DR AlphaFoldDB; O75954; -.
DR SMR; O75954; -.
DR BioGRID; 116076; 7.
DR IntAct; O75954; 4.
DR MINT; O75954; -.
DR STRING; 9606.ENSP00000011898; -.
DR TCDB; 8.A.40.1.22; the tetraspanin (tetraspanin) family.
DR GlyGen; O75954; 1 site.
DR PhosphoSitePlus; O75954; -.
DR SwissPalm; O75954; -.
DR BioMuta; TSPAN9; -.
DR EPD; O75954; -.
DR jPOST; O75954; -.
DR MassIVE; O75954; -.
DR MaxQB; O75954; -.
DR PaxDb; O75954; -.
DR PeptideAtlas; O75954; -.
DR PRIDE; O75954; -.
DR ProteomicsDB; 50320; -.
DR Antibodypedia; 2820; 165 antibodies from 32 providers.
DR DNASU; 10867; -.
DR Ensembl; ENST00000011898.10; ENSP00000011898.5; ENSG00000011105.14.
DR Ensembl; ENST00000537971.5; ENSP00000444799.1; ENSG00000011105.14.
DR GeneID; 10867; -.
DR KEGG; hsa:10867; -.
DR MANE-Select; ENST00000011898.10; ENSP00000011898.5; NM_006675.5; NP_006666.1.
DR UCSC; uc001qlp.4; human.
DR CTD; 10867; -.
DR DisGeNET; 10867; -.
DR GeneCards; TSPAN9; -.
DR HGNC; HGNC:21640; TSPAN9.
DR HPA; ENSG00000011105; Tissue enhanced (heart).
DR MIM; 613137; gene.
DR neXtProt; NX_O75954; -.
DR OpenTargets; ENSG00000011105; -.
DR PharmGKB; PA142670694; -.
DR VEuPathDB; HostDB:ENSG00000011105; -.
DR eggNOG; KOG3882; Eukaryota.
DR GeneTree; ENSGT00940000158225; -.
DR HOGENOM; CLU_055524_4_3_1; -.
DR InParanoid; O75954; -.
DR OMA; DIQSWSG; -.
DR OrthoDB; 1224210at2759; -.
DR PhylomeDB; O75954; -.
DR TreeFam; TF352892; -.
DR PathwayCommons; O75954; -.
DR SignaLink; O75954; -.
DR BioGRID-ORCS; 10867; 7 hits in 1074 CRISPR screens.
DR ChiTaRS; TSPAN9; human.
DR GenomeRNAi; 10867; -.
DR Pharos; O75954; Tbio.
DR PRO; PR:O75954; -.
DR Proteomes; UP000005640; Chromosome 12.
DR RNAct; O75954; protein.
DR Bgee; ENSG00000011105; Expressed in apex of heart and 192 other tissues.
DR ExpressionAtlas; O75954; baseline and differential.
DR Genevisible; O75954; HS.
DR GO; GO:0005925; C:focal adhesion; HDA:UniProtKB.
DR GO; GO:0005887; C:integral component of plasma membrane; IBA:GO_Central.
DR GO; GO:0005886; C:plasma membrane; IDA:UniProtKB.
DR GO; GO:0097197; C:tetraspanin-enriched microdomain; IDA:UniProtKB.
DR Gene3D; 1.10.1450.10; -; 1.
DR InterPro; IPR018499; Tetraspanin/Peripherin.
DR InterPro; IPR000301; Tetraspanin_animals.
DR InterPro; IPR018503; Tetraspanin_CS.
DR InterPro; IPR008952; Tetraspanin_EC2_sf.
DR PANTHER; PTHR19282; PTHR19282; 1.
DR Pfam; PF00335; Tetraspanin; 1.
DR PIRSF; PIRSF002419; Tetraspanin; 1.
DR SUPFAM; SSF48652; SSF48652; 1.
DR PROSITE; PS00421; TM4_1; 1.
PE 1: Evidence at protein level;
KW Glycoprotein; Membrane; Reference proteome; Transmembrane;
KW Transmembrane helix.
FT CHAIN 1..239
FT /note="Tetraspanin-9"
FT /id="PRO_0000219253"
FT TOPO_DOM 1..13
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 14..34
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 35..55
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 56..76
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 77..85
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 86..106
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 107..203
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 204..224
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 225..239
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT CARBOHYD 180
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CONFLICT 216
FT /note="Q -> R (in Ref. 4; BAD96899)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 239 AA; 26779 MW; DD7BA332BF6584EB CRC64;
MARGCLCCLK YMMFLFNLIF WLCGCGLLGV GIWLSVSQGN FATFSPSFPS LSAANLVIAI
GTIVMVTGFL GCLGAIKENK CLLLSFFIVL LVILLAELIL LILFFVYMDK VNENAKKDLK
EGLLLYHTEN NVGLKNAWNI IQAEMRCCGV TDYTDWYPVL GENTVPDRCC MENSQGCGRN
ATTPLWRTGC YEKVKMWFDD NKHVLGTVGM CILIMQILGM AFSMTLFQHI HRTGKKYDA
