TSN9_MOUSE
ID TSN9_MOUSE Reviewed; 239 AA.
AC Q8BJU2; Q3TQQ0;
DT 26-APR-2005, integrated into UniProtKB/Swiss-Prot.
DT 01-MAR-2003, sequence version 1.
DT 03-AUG-2022, entry version 135.
DE RecName: Full=Tetraspanin-9;
DE Short=Tspan-9;
DE AltName: Full=Tetraspan NET-5;
GN Name=Tspan9; Synonyms=Net5;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=C57BL/6J; TISSUE=Embryo, and Visual cortex;
RX PubMed=16141072; DOI=10.1126/science.1112014;
RA Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N.,
RA Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K.,
RA Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.,
RA Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R.,
RA Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T.,
RA Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A.,
RA Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B.,
RA Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M.,
RA Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S.,
RA Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E.,
RA Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D.,
RA Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M.,
RA Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H.,
RA Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V.,
RA Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S.,
RA Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H.,
RA Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N.,
RA Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F.,
RA Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G.,
RA Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z.,
RA Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C.,
RA Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y.,
RA Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S.,
RA Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K.,
RA Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R.,
RA van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H.,
RA Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M.,
RA Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C.,
RA Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S.,
RA Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K.,
RA Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M.,
RA Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C.,
RA Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A.,
RA Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.;
RT "The transcriptional landscape of the mammalian genome.";
RL Science 309:1559-1563(2005).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=Czech II; TISSUE=Lung;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [3]
RP IDENTIFICATION IN A COMPLEX WITH GP6, SUBCELLULAR LOCATION, GLYCOSYLATION,
RP AND TISSUE SPECIFICITY.
RX PubMed=18795891; DOI=10.1042/bj20081126;
RA Protty M.B., Watkins N.A., Colombo D., Thomas S.G., Heath V.L.,
RA Herbert J.M., Bicknell R., Senis Y.A., Ashman L.K., Berditchevski F.,
RA Ouwehand W.H., Watson S.P., Tomlinson M.G.;
RT "Identification of Tspan9 as a novel platelet tetraspanin and the collagen
RT receptor GPVI as a component of tetraspanin microdomains.";
RL Biochem. J. 417:391-400(2009).
RN [4]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Heart, Kidney, Liver, Lung, and Spleen;
RX PubMed=21183079; DOI=10.1016/j.cell.2010.12.001;
RA Huttlin E.L., Jedrychowski M.P., Elias J.E., Goswami T., Rad R.,
RA Beausoleil S.A., Villen J., Haas W., Sowa M.E., Gygi S.P.;
RT "A tissue-specific atlas of mouse protein phosphorylation and expression.";
RL Cell 143:1174-1189(2010).
CC -!- SUBUNIT: Found in a complex with GP6. {ECO:0000269|PubMed:18795891}.
CC -!- SUBCELLULAR LOCATION: Membrane {ECO:0000305|PubMed:18795891}; Multi-
CC pass membrane protein {ECO:0000305|PubMed:18795891}. Note=Colocalizes
CC with GP6 in tetraspanin microdomains on the platelet surface.
CC -!- TISSUE SPECIFICITY: Strongly expressed in megakaryocytes, platelets and
CC lung. Weakly expressed in bone marrow, brain and kidney (at protein
CC level). {ECO:0000269|PubMed:18795891}.
CC -!- PTM: Glycosylated. {ECO:0000269|PubMed:18795891}.
CC -!- SIMILARITY: Belongs to the tetraspanin (TM4SF) family. {ECO:0000305}.
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DR EMBL; AK079139; BAC37559.1; -; mRNA.
DR EMBL; AK158729; BAE34632.1; -; mRNA.
DR EMBL; AK163395; BAE37332.1; -; mRNA.
DR EMBL; BC052503; AAH52503.1; -; mRNA.
DR CCDS; CCDS20568.1; -.
DR RefSeq; NP_780623.1; NM_175414.4.
DR RefSeq; XP_006505396.1; XM_006505333.3.
DR RefSeq; XP_006505398.1; XM_006505335.2.
DR AlphaFoldDB; Q8BJU2; -.
DR SMR; Q8BJU2; -.
DR BioGRID; 224615; 1.
DR STRING; 10090.ENSMUSP00000107794; -.
DR GlyGen; Q8BJU2; 1 site.
DR iPTMnet; Q8BJU2; -.
DR PhosphoSitePlus; Q8BJU2; -.
DR SwissPalm; Q8BJU2; -.
DR jPOST; Q8BJU2; -.
DR MaxQB; Q8BJU2; -.
DR PaxDb; Q8BJU2; -.
DR PeptideAtlas; Q8BJU2; -.
DR PRIDE; Q8BJU2; -.
DR ProteomicsDB; 297666; -.
DR Antibodypedia; 2820; 165 antibodies from 32 providers.
DR DNASU; 109246; -.
DR Ensembl; ENSMUST00000032503; ENSMUSP00000032503; ENSMUSG00000030352.
DR Ensembl; ENSMUST00000112171; ENSMUSP00000107794; ENSMUSG00000030352.
DR Ensembl; ENSMUST00000112173; ENSMUSP00000107796; ENSMUSG00000030352.
DR GeneID; 109246; -.
DR KEGG; mmu:109246; -.
DR UCSC; uc009eco.1; mouse.
DR CTD; 10867; -.
DR MGI; MGI:1924558; Tspan9.
DR VEuPathDB; HostDB:ENSMUSG00000030352; -.
DR eggNOG; KOG3882; Eukaryota.
DR GeneTree; ENSGT00940000158225; -.
DR HOGENOM; CLU_055524_4_3_1; -.
DR InParanoid; Q8BJU2; -.
DR OMA; DIQSWSG; -.
DR OrthoDB; 1224210at2759; -.
DR PhylomeDB; Q8BJU2; -.
DR TreeFam; TF352892; -.
DR BioGRID-ORCS; 109246; 3 hits in 74 CRISPR screens.
DR ChiTaRS; Tspan9; mouse.
DR PRO; PR:Q8BJU2; -.
DR Proteomes; UP000000589; Chromosome 6.
DR RNAct; Q8BJU2; protein.
DR Bgee; ENSMUSG00000030352; Expressed in gastrula and 265 other tissues.
DR ExpressionAtlas; Q8BJU2; baseline and differential.
DR Genevisible; Q8BJU2; MM.
DR GO; GO:0005887; C:integral component of plasma membrane; IBA:GO_Central.
DR GO; GO:0016020; C:membrane; IDA:UniProtKB.
DR GO; GO:0005886; C:plasma membrane; ISO:MGI.
DR GO; GO:0097197; C:tetraspanin-enriched microdomain; ISO:MGI.
DR Gene3D; 1.10.1450.10; -; 1.
DR InterPro; IPR018499; Tetraspanin/Peripherin.
DR InterPro; IPR000301; Tetraspanin_animals.
DR InterPro; IPR018503; Tetraspanin_CS.
DR InterPro; IPR008952; Tetraspanin_EC2_sf.
DR PANTHER; PTHR19282; PTHR19282; 1.
DR Pfam; PF00335; Tetraspanin; 1.
DR PIRSF; PIRSF002419; Tetraspanin; 1.
DR SUPFAM; SSF48652; SSF48652; 1.
DR PROSITE; PS00421; TM4_1; 1.
PE 1: Evidence at protein level;
KW Glycoprotein; Membrane; Reference proteome; Transmembrane;
KW Transmembrane helix.
FT CHAIN 1..239
FT /note="Tetraspanin-9"
FT /id="PRO_0000219254"
FT TOPO_DOM 1..13
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 14..34
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 35..55
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 56..76
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 77..85
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 86..106
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 107..203
FT /note="Extracellular"
FT /evidence="ECO:0000255"
FT TRANSMEM 204..224
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 225..239
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT CARBOHYD 180
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000269|PubMed:18795891"
SQ SEQUENCE 239 AA; 26738 MW; B3DC47ADA1D877DD CRC64;
MARGCLCCLK YTMFLFNLIF WLCGCGLLGV GIWLSVSQGN FATFSPSFPS LSAANLVIAI
GTIVMVTGFL GCLGAIKENK CLLLSFFIVL LIILLAELIL IILFFVYMDK VNENAKQDLK
EGLLLYNTEN NVGLKNAWNI IQAEMRCCGV TDYTDWYPVL GENTVPDRCC MENSQGCGRN
STTPLWRTGC YEKVKLWFDD NKHVLGTVGM CILIMQILGM AFSMTLFQHI HRTGKKYDA
