TSNA1_HUMAN
ID TSNA1_HUMAN Reviewed; 513 AA.
AC Q96NA8; B7ZLB0; Q14D03;
DT 13-SEP-2005, integrated into UniProtKB/Swiss-Prot.
DT 05-MAY-2009, sequence version 2.
DT 03-AUG-2022, entry version 137.
DE RecName: Full=t-SNARE domain-containing protein 1;
GN Name=TSNARE1;
OS Homo sapiens (Human).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae;
OC Homo.
OX NCBI_TaxID=9606;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1), AND VARIANTS LEU-18;
RP PRO-55 AND ALA-118.
RC TISSUE=Kidney;
RX PubMed=14702039; DOI=10.1038/ng1285;
RA Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R.,
RA Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H.,
RA Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.,
RA Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K.,
RA Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H.,
RA Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M.,
RA Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K.,
RA Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T.,
RA Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M.,
RA Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S.,
RA Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H.,
RA Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K.,
RA Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N.,
RA Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S.,
RA Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O.,
RA Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H.,
RA Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B.,
RA Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y.,
RA Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K.,
RA Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T.,
RA Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T.,
RA Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y.,
RA Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H.,
RA Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y.,
RA Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H.,
RA Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O.,
RA Isogai T., Sugano S.;
RT "Complete sequencing and characterization of 21,243 full-length human
RT cDNAs.";
RL Nat. Genet. 36:40-45(2004).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX PubMed=16421571; DOI=10.1038/nature04406;
RA Nusbaum C., Mikkelsen T.S., Zody M.C., Asakawa S., Taudien S., Garber M.,
RA Kodira C.D., Schueler M.G., Shimizu A., Whittaker C.A., Chang J.L.,
RA Cuomo C.A., Dewar K., FitzGerald M.G., Yang X., Allen N.R., Anderson S.,
RA Asakawa T., Blechschmidt K., Bloom T., Borowsky M.L., Butler J., Cook A.,
RA Corum B., DeArellano K., DeCaprio D., Dooley K.T., Dorris L. III,
RA Engels R., Gloeckner G., Hafez N., Hagopian D.S., Hall J.L., Ishikawa S.K.,
RA Jaffe D.B., Kamat A., Kudoh J., Lehmann R., Lokitsang T., Macdonald P.,
RA Major J.E., Matthews C.D., Mauceli E., Menzel U., Mihalev A.H.,
RA Minoshima S., Murayama Y., Naylor J.W., Nicol R., Nguyen C., O'Leary S.B.,
RA O'Neill K., Parker S.C.J., Polley A., Raymond C.K., Reichwald K.,
RA Rodriguez J., Sasaki T., Schilhabel M., Siddiqui R., Smith C.L.,
RA Sneddon T.P., Talamas J.A., Tenzin P., Topham K., Venkataraman V., Wen G.,
RA Yamazaki S., Young S.K., Zeng Q., Zimmer A.R., Rosenthal A., Birren B.W.,
RA Platzer M., Shimizu N., Lander E.S.;
RT "DNA sequence and analysis of human chromosome 8.";
RL Nature 439:331-335(2006).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
RC TISSUE=Brain;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [4]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-378, AND IDENTIFICATION BY
RP MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Erythroleukemia;
RX PubMed=23186163; DOI=10.1021/pr300630k;
RA Zhou H., Di Palma S., Preisinger C., Peng M., Polat A.N., Heck A.J.,
RA Mohammed S.;
RT "Toward a comprehensive characterization of a human cancer cell
RT phosphoproteome.";
RL J. Proteome Res. 12:260-271(2013).
CC -!- SUBCELLULAR LOCATION: Membrane {ECO:0000250}; Single-pass membrane
CC protein {ECO:0000250}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=1;
CC IsoId=Q96NA8-1; Sequence=Displayed;
CC Name=2;
CC IsoId=Q96NA8-2; Sequence=VSP_056304;
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DR EMBL; AK055726; BAB70997.1; -; mRNA.
DR EMBL; AC103758; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; AC129918; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; AC134682; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; BC113556; AAI13557.1; -; mRNA.
DR EMBL; BC143684; AAI43685.1; -; mRNA.
DR CCDS; CCDS6384.1; -. [Q96NA8-1]
DR RefSeq; NP_659440.2; NM_145003.4. [Q96NA8-1]
DR RefSeq; XP_016868667.1; XM_017013178.1.
DR AlphaFoldDB; Q96NA8; -.
DR SMR; Q96NA8; -.
DR BioGRID; 128443; 37.
DR IntAct; Q96NA8; 2.
DR STRING; 9606.ENSP00000303437; -.
DR iPTMnet; Q96NA8; -.
DR PhosphoSitePlus; Q96NA8; -.
DR BioMuta; TSNARE1; -.
DR DMDM; 229462801; -.
DR EPD; Q96NA8; -.
DR jPOST; Q96NA8; -.
DR MassIVE; Q96NA8; -.
DR PaxDb; Q96NA8; -.
DR PeptideAtlas; Q96NA8; -.
DR PRIDE; Q96NA8; -.
DR ProteomicsDB; 7219; -.
DR ProteomicsDB; 77496; -. [Q96NA8-1]
DR Antibodypedia; 14519; 87 antibodies from 14 providers.
DR DNASU; 203062; -.
DR Ensembl; ENST00000524325.6; ENSP00000428763.2; ENSG00000171045.16. [Q96NA8-1]
DR GeneID; 203062; -.
DR KEGG; hsa:203062; -.
DR MANE-Select; ENST00000524325.6; ENSP00000428763.2; NM_145003.5; NP_659440.2.
DR UCSC; uc003ywk.4; human. [Q96NA8-1]
DR CTD; 203062; -.
DR DisGeNET; 203062; -.
DR GeneCards; TSNARE1; -.
DR HGNC; HGNC:26437; TSNARE1.
DR HPA; ENSG00000171045; Low tissue specificity.
DR neXtProt; NX_Q96NA8; -.
DR OpenTargets; ENSG00000171045; -.
DR PharmGKB; PA142670693; -.
DR VEuPathDB; HostDB:ENSG00000171045; -.
DR eggNOG; KOG0811; Eukaryota.
DR GeneTree; ENSGT01000000214440; -.
DR InParanoid; Q96NA8; -.
DR OMA; IQHYGVV; -.
DR OrthoDB; 182700at2759; -.
DR PhylomeDB; Q96NA8; -.
DR TreeFam; TF335542; -.
DR PathwayCommons; Q96NA8; -.
DR SignaLink; Q96NA8; -.
DR BioGRID-ORCS; 203062; 10 hits in 1078 CRISPR screens.
DR ChiTaRS; TSNARE1; human.
DR GenomeRNAi; 203062; -.
DR Pharos; Q96NA8; Tdark.
DR PRO; PR:Q96NA8; -.
DR Proteomes; UP000005640; Chromosome 8.
DR RNAct; Q96NA8; protein.
DR Bgee; ENSG00000171045; Expressed in skin of abdomen and 120 other tissues.
DR ExpressionAtlas; Q96NA8; baseline and differential.
DR Genevisible; Q96NA8; HS.
DR GO; GO:0012505; C:endomembrane system; IBA:GO_Central.
DR GO; GO:0016021; C:integral component of membrane; IBA:GO_Central.
DR GO; GO:0031201; C:SNARE complex; IBA:GO_Central.
DR GO; GO:0005484; F:SNAP receptor activity; IBA:GO_Central.
DR GO; GO:0000149; F:SNARE binding; IBA:GO_Central.
DR GO; GO:0006886; P:intracellular protein transport; IBA:GO_Central.
DR GO; GO:0048278; P:vesicle docking; IBA:GO_Central.
DR GO; GO:0006906; P:vesicle fusion; IBA:GO_Central.
DR InterPro; IPR028002; Myb_DNA-bind_5.
DR InterPro; IPR010989; SNARE.
DR InterPro; IPR045242; Syntaxin.
DR InterPro; IPR006011; Syntaxin_N.
DR InterPro; IPR000727; T_SNARE_dom.
DR PANTHER; PTHR19957; PTHR19957; 1.
DR Pfam; PF13873; Myb_DNA-bind_5; 1.
DR Pfam; PF05739; SNARE; 1.
DR Pfam; PF14523; Syntaxin_2; 1.
DR SMART; SM00503; SynN; 1.
DR SMART; SM00397; t_SNARE; 1.
DR SUPFAM; SSF47661; SSF47661; 1.
DR PROSITE; PS50192; T_SNARE; 1.
PE 1: Evidence at protein level;
KW Alternative splicing; Coiled coil; Membrane; Phosphoprotein;
KW Reference proteome; Transmembrane; Transmembrane helix.
FT CHAIN 1..513
FT /note="t-SNARE domain-containing protein 1"
FT /id="PRO_0000065668"
FT TRANSMEM 491..511
FT /note="Helical"
FT /evidence="ECO:0000255"
FT DOMAIN 416..478
FT /note="t-SNARE coiled-coil homology"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00202"
FT REGION 1..23
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 49..128
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOD_RES 378
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:23186163"
FT VAR_SEQ 329
FT /note="Missing (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:15489334"
FT /id="VSP_056304"
FT VARIANT 18
FT /note="F -> L (in dbSNP:rs7814359)"
FT /evidence="ECO:0000269|PubMed:14702039"
FT /id="VAR_055146"
FT VARIANT 55
FT /note="R -> P (in dbSNP:rs33970858)"
FT /evidence="ECO:0000269|PubMed:14702039"
FT /id="VAR_051461"
FT VARIANT 118
FT /note="T -> A (in dbSNP:rs10100935)"
FT /evidence="ECO:0000269|PubMed:14702039"
FT /id="VAR_051462"
FT VARIANT 268
FT /note="V -> I (in dbSNP:rs10435683)"
FT /id="VAR_051463"
SQ SEQUENCE 513 AA; 55949 MW; 415F67290CF97DA8 CRC64;
MSYGSIARGG GLGSRGPFGG PSRQGCQPLE CARCWTEYGI RHFPCPSPES KLQNRCVGKD
GEGDLGPAGT PIVPRARKRG PGVAPEGSRM PEPTSSPTIG PRKDSAAGPH GRMAGPSTTR
AKKRKPNFCP QETEVLVSKV SKHHQLLFGT GLLKAEPTRR YRVWSRILQA VNALGYCRRD
VVDLKHKWRD LRAVVRRKLG DLRKAAHGPS PGSGKPQALA LTPVEQVVAK TFSCQALPSE
GFSLEPPRAT QVDPCNLQEL FQEMSANVFR INSSVTSLER SLQSLGTPSD TQELRDSLHT
AQQETNKTIA ASASSVKQMA ELLRSSCPQE RLQQERPQLD RLKTQLSDAI QCYGVVQKKI
AEKSRALLPM AQRGSKQSPQ APFAELADDE KVFNGSDNMW QGQEQALLPD ITEEDLEAIR
LREEAILQME SNLLDVNQII KDLASMVSEQ GEAVDSIEAS LEAASSHAEA ARQLLAGASR
HQLQRHKIKC CFLSAGVTAL LVIIIIIATS VRK
