TSO1_ARATH
ID TSO1_ARATH Reviewed; 695 AA.
AC Q9LUI3; Q8H1Q0; Q94AI2; Q9LE32;
DT 11-JUL-2012, integrated into UniProtKB/Swiss-Prot.
DT 01-OCT-2000, sequence version 1.
DT 03-AUG-2022, entry version 114.
DE RecName: Full=CRC domain-containing protein TSO1;
GN Name=TSO1; OrderedLocusNames=At3g22780; ORFNames=MWI23.15;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, TISSUE SPECIFICITY, MUTAGENESIS OF
RP CYS-403, AND SUBCELLULAR LOCATION.
RC STRAIN=cv. Landsberg erecta; TISSUE=Flower;
RX PubMed=10769244; DOI=10.1242/dev.127.10.2207;
RA Song J.Y., Leung T., Ehler L.K., Wang C., Liu Z.;
RT "Regulation of meristem organization and cell division by TSO1, an
RT Arabidopsis gene with cysteine-rich repeats.";
RL Development 127:2207-2217(2000).
RN [2]
RP NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, TISSUE SPECIFICITY, MUTAGENESIS OF
RP CYS-501, AND SUBCELLULAR LOCATION.
RC STRAIN=cv. Landsberg erecta;
RX PubMed=10769245; DOI=10.1242/dev.127.10.2219;
RA Hauser B.A., He J.Q., Park S.O., Gasser C.S.;
RT "TSO1 is a novel protein that modulates cytokinesis and cell expansion in
RT Arabidopsis.";
RL Development 127:2219-2226(2000).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=10819329; DOI=10.1093/dnares/7.2.131;
RA Sato S., Nakamura Y., Kaneko T., Katoh T., Asamizu E., Tabata S.;
RT "Structural analysis of Arabidopsis thaliana chromosome 3. I. Sequence
RT features of the regions of 4,504,864 bp covered by sixty P1 and TAC
RT clones.";
RL DNA Res. 7:131-135(2000).
RN [4]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 160-695.
RC STRAIN=cv. Columbia;
RX PubMed=14593172; DOI=10.1126/science.1088305;
RA Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M.,
RA Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G.,
RA Liu S.X., Lam B., Sakano H., Wu T., Yu G., Miranda M., Quach H.L.,
RA Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C.,
RA Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J.,
RA Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A.,
RA Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C.,
RA Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X.,
RA Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M.,
RA Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K.,
RA Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A.,
RA Ecker J.R.;
RT "Empirical analysis of transcriptional activity in the Arabidopsis
RT genome.";
RL Science 302:842-846(2003).
RN [6]
RP FUNCTION.
RX PubMed=9043081; DOI=10.1242/dev.124.3.665;
RA Liu Z., Running M.P., Meyerowitz E.M.;
RT "TSO1 functions in cell division during Arabidopsis flower development.";
RL Development 124:665-672(1997).
RN [7]
RP FUNCTION, AND DISRUPTION PHENOTYPE.
RX PubMed=9725857; DOI=10.1093/genetics/150.1.411;
RA Hauser B.A., Villanueva J.M., Gasser C.S.;
RT "Arabidopsis TSO1 regulates directional processes in cells during floral
RT organogenesis.";
RL Genetics 150:411-423(1998).
RN [8]
RP GENE FAMILY, NOMENCLATURE, ZINC-BINDING, DISRUPTION PHENOTYPE, TISSUE
RP SPECIFICITY, AND DEVELOPMENTAL STAGE.
RX PubMed=18057042; DOI=10.1093/jxb/erm215;
RA Andersen S.U., Algreen-Petersen R.G., Hoedl M., Jurkiewicz A.,
RA Cvitanich C., Braunschweig U., Schauser L., Oh S.A., Twell D., Jensen E.O.;
RT "The conserved cysteine-rich domain of a tesmin/TSO1-like protein binds
RT zinc in vitro and TSO1 is required for both male and female fertility in
RT Arabidopsis thaliana.";
RL J. Exp. Bot. 58:3657-3670(2007).
RN [9]
RP DISRUPTION PHENOTYPE.
RX PubMed=22072982; DOI=10.1371/journal.pgen.1002352;
RA Sijacic P., Wang W., Liu Z.;
RT "Recessive antimorphic alleles overcome functionally redundant loci to
RT reveal TSO1 function in Arabidopsis flowers and meristems.";
RL PLoS Genet. 7:E1002352-E1002352(2011).
CC -!- FUNCTION: Probable floral-specific cell division component, required
CC for proper organ formation in flowers. Regulates the floral meristem
CC cell division and the inflorescence meristem organization. Plays a role
CC in development of both male and female reproductive tissues.
CC {ECO:0000269|PubMed:10769244, ECO:0000269|PubMed:10769245,
CC ECO:0000269|PubMed:9043081, ECO:0000269|PubMed:9725857}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000269|PubMed:10769244,
CC ECO:0000269|PubMed:10769245}.
CC -!- TISSUE SPECIFICITY: Ubiquitous but expressed mostly in flowers with
CC highest levels in developing ovules and microspores.
CC {ECO:0000269|PubMed:10769244, ECO:0000269|PubMed:10769245,
CC ECO:0000269|PubMed:18057042}.
CC -!- DEVELOPMENTAL STAGE: Expressed at the first stage of pollen development
CC in uninucleate microspores and bicellular pollen but not in tricellular
CC and mature pollen. {ECO:0000269|PubMed:18057042}.
CC -!- DOMAIN: The cysteine-rich domain CRC binds zinc in vitro.
CC -!- DISRUPTION PHENOTYPE: Despite a wild-type flower phenotype, the null
CC mutant presents defects in pollen, carpel and ovule development,
CC including a failure in megasporogenesis. Small siliques.
CC {ECO:0000269|PubMed:18057042, ECO:0000269|PubMed:22072982,
CC ECO:0000269|PubMed:9725857}.
CC -!- SIMILARITY: Belongs to the lin-54 family. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAK76693.1; Type=Erroneous initiation; Note=Truncated N-terminus.; Evidence={ECO:0000305};
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DR EMBL; AF206324; AAF27433.1; -; mRNA.
DR EMBL; AF204059; AAF69124.1; -; mRNA.
DR EMBL; AB022223; BAB01253.1; -; Genomic_DNA.
DR EMBL; CP002686; AEE76676.1; -; Genomic_DNA.
DR EMBL; AY046019; AAK76693.1; ALT_INIT; mRNA.
DR EMBL; AY142642; AAN13100.1; -; mRNA.
DR RefSeq; NP_566718.2; NM_113177.4.
DR AlphaFoldDB; Q9LUI3; -.
DR SMR; Q9LUI3; -.
DR STRING; 3702.AT3G22780.1; -.
DR iPTMnet; Q9LUI3; -.
DR PaxDb; Q9LUI3; -.
DR PRIDE; Q9LUI3; -.
DR ProteomicsDB; 232356; -.
DR EnsemblPlants; AT3G22780.1; AT3G22780.1; AT3G22780.
DR GeneID; 821849; -.
DR Gramene; AT3G22780.1; AT3G22780.1; AT3G22780.
DR KEGG; ath:AT3G22780; -.
DR Araport; AT3G22780; -.
DR TAIR; locus:2094369; AT3G22780.
DR eggNOG; KOG1171; Eukaryota.
DR HOGENOM; CLU_012297_1_0_1; -.
DR InParanoid; Q9LUI3; -.
DR OMA; RCHNSVD; -.
DR OrthoDB; 267145at2759; -.
DR PhylomeDB; Q9LUI3; -.
DR PRO; PR:Q9LUI3; -.
DR Proteomes; UP000006548; Chromosome 3.
DR ExpressionAtlas; Q9LUI3; baseline and differential.
DR Genevisible; Q9LUI3; AT.
DR GO; GO:0005634; C:nucleus; IDA:UniProtKB.
DR GO; GO:0003700; F:DNA-binding transcription factor activity; ISS:TAIR.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR GO; GO:0000976; F:transcription cis-regulatory region binding; IPI:TAIR.
DR GO; GO:0010481; P:epidermal cell division; IGI:TAIR.
DR GO; GO:0048444; P:floral organ morphogenesis; IMP:UniProtKB.
DR GO; GO:0010235; P:guard mother cell cytokinesis; IGI:TAIR.
DR GO; GO:0051302; P:regulation of cell division; IMP:UniProtKB.
DR GO; GO:0009934; P:regulation of meristem structural organization; IMP:TAIR.
DR InterPro; IPR005172; CRC.
DR InterPro; IPR033467; Tesmin/TSO1-like_CXC.
DR InterPro; IPR044522; TSO1-like.
DR PANTHER; PTHR46159; PTHR46159; 1.
DR Pfam; PF03638; TCR; 2.
DR SMART; SM01114; CXC; 2.
DR PROSITE; PS51634; CRC; 1.
PE 1: Evidence at protein level;
KW Developmental protein; Metal-binding; Nucleus; Reference proteome; Zinc.
FT CHAIN 1..695
FT /note="CRC domain-containing protein TSO1"
FT /id="PRO_0000418166"
FT DOMAIN 399..524
FT /note="CRC"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00971"
FT REGION 1..21
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 107..128
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 559..578
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 599..626
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 650..676
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOTIF 269..283
FT /note="Nuclear localization signal"
FT /evidence="ECO:0000255"
FT COMPBIAS 599..622
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 650..671
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MUTAGEN 403
FT /note="C->Y: Abnormalities in floral meristems and sepals
FT including increased cell size, decreased cell number,
FT disorganized layer structure, increased DNA content per
FT cell, and partially formed cell walls. Resulting phenotype
FT is callus-like tissues in place of floral organs."
FT /evidence="ECO:0000269|PubMed:10769244"
FT MUTAGEN 501
FT /note="C->Y: Abnormalities in floral meristems and sepals
FT including increased cell size, decreased cell number,
FT disorganized layer structure, increased DNA content per
FT cell, and partially formed cell walls. Resulting phenotype
FT is callus-like tissues in place of floral organs."
FT /evidence="ECO:0000269|PubMed:10769245"
FT CONFLICT 130
FT /note="M -> L (in Ref. 1; AAF27433 and 2; AAF69124)"
FT /evidence="ECO:0000305"
FT CONFLICT 375
FT /note="V -> M (in Ref. 5; AAK76693)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 695 AA; 76277 MW; 34BBA0E450F6BCE1 CRC64;
MDKSQKNPTS QIGTSTPKSK FEDSPVFNYI SNLSPIESVK SISTAQTFSS LSFTSPPPVF
TSPHVISHRE SRFFRCHNSV DRSKHLESLD GSAVKGEVVV PLVEDLNKEA SLEDEEETSV
ETSSELPQIM KFDSQTSEHS DSPCTEDVVI EASSDPPRGD NGSSSEDVTM GLQNMLVVRE
GNDTPGCGRL ISDATELLVF RSPNDSEAFR CLVDKISSSE RRFCAGVKST KRPDINKDIP
ANGSSNENQP LAVLPTNESV FNLHRGGMRR RCLDFEMPGK RKKDIVDDQQ SVCDNNVAGE
SSSSCVVPGI GLHLNAVAMS AKDSNISVIH GYSISGEIQK SFSGSTTPIQ SQDTVQETSD
QAENEPVEEV PKALVFPELN LGSLKKKMRK SEQAGEGESC KRCNCKKSKC LKLYCECFAA
GVYCIEPCSC IDCFNKPIHE ETVLATRKQI ESRNPLAFAP KVIRNADSIM EASDDASKTP
ASARHKRGCN CKKSNCMKKY CECYQGGVGC SMNCRCEGCT NVFGRKDGSL LVIMESKLEE
NQETYEKRIA KIQHNVEVSK EVEQNPSSDQ PSTPLPPYRH LVVHQPFLSK NRLPPTQFFL
GTGSSSFRKP NSDLAQSQNE KKPLETVTED KTEIMPEILL NSPIANIKAI SPNSKRVSPP
QPGSSESGSI LRRRGNGRKL ILRSIPAFPS LNPNQ
