TSPY1_RAT
ID TSPY1_RAT Reviewed; 334 AA.
AC Q9R1M3; O70602; Q9R1M4;
DT 15-AUG-2003, integrated into UniProtKB/Swiss-Prot.
DT 15-AUG-2003, sequence version 2.
DT 03-AUG-2022, entry version 106.
DE RecName: Full=Testis-specific Y-encoded protein 1;
DE Short=rTSPY;
GN Name=Tspy1; Synonyms=Tspy;
OS Rattus norvegicus (Rat).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Rattus.
OX NCBI_TaxID=10116;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA] (ISOFORMS 1 AND 2).
RC STRAIN=Sprague-Dawley; TISSUE=Testis;
RX PubMed=10072602; DOI=10.1159/000015169;
RA Dechend F., Schubert S., Nanda I., Vogel T., Schmid M., Schmidtke J.;
RT "Organization and expression of rat Tspy.";
RL Cytogenet. Cell Genet. 83:270-274(1998).
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA / MRNA] OF 163-334 (ISOFORM 1), AND TISSUE
RP SPECIFICITY.
RC STRAIN=Wistar; TISSUE=Testis;
RX PubMed=9467017; DOI=10.1093/hmg/7.3.557;
RA Mazeyrat S., Mitchell M.J.;
RT "Rodent Y chromosome TSPY gene is functional in rat and non-functional in
RT mouse.";
RL Hum. Mol. Genet. 7:557-562(1998).
RN [3]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-4, AND IDENTIFICATION BY MASS
RP SPECTROMETRY [LARGE SCALE ANALYSIS].
RX PubMed=22673903; DOI=10.1038/ncomms1871;
RA Lundby A., Secher A., Lage K., Nordsborg N.B., Dmytriyev A., Lundby C.,
RA Olsen J.V.;
RT "Quantitative maps of protein phosphorylation sites across 14 different rat
RT organs and tissues.";
RL Nat. Commun. 3:876-876(2012).
CC -!- FUNCTION: May be involved in sperm differentiation and proliferation.
CC {ECO:0000250|UniProtKB:Q01534}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000250|UniProtKB:Q01534}. Nucleus
CC {ECO:0000250|UniProtKB:Q01534}. Note=Predominantly cytoplasmic. Also
CC found in nucleus. {ECO:0000250|UniProtKB:Q01534}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=1; Synonyms=pLEE;
CC IsoId=Q9R1M3-1; Sequence=Displayed;
CC Name=2; Synonyms=pSEE;
CC IsoId=Q9R1M3-2; Sequence=VSP_008014, VSP_008015;
CC -!- TISSUE SPECIFICITY: Testis. {ECO:0000269|PubMed:9467017}.
CC -!- PTM: Phosphorylated. {ECO:0000250}.
CC -!- SIMILARITY: Belongs to the nucleosome assembly protein (NAP) family.
CC {ECO:0000305}.
CC -!- CAUTION: Maps to duplicated regions on chromosome Y; the gene is
CC present in at least 3 copies. {ECO:0000305}.
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DR EMBL; AF074880; AAD42924.1; -; mRNA.
DR EMBL; AF074879; AAD42694.1; -; Genomic_DNA.
DR EMBL; AJ001377; CAA04709.1; -; mRNA.
DR EMBL; AJ001380; CAA04711.1; -; Genomic_DNA.
DR RefSeq; NP_075212.1; NM_022923.1. [Q9R1M3-1]
DR RefSeq; XP_008771894.1; XM_008773672.2. [Q9R1M3-1]
DR AlphaFoldDB; Q9R1M3; -.
DR SMR; Q9R1M3; -.
DR BioGRID; 247264; 2.
DR IntAct; Q9R1M3; 1.
DR iPTMnet; Q9R1M3; -.
DR PhosphoSitePlus; Q9R1M3; -.
DR Ensembl; ENSRNOT00000083382; ENSRNOP00000075045; ENSRNOG00000054648. [Q9R1M3-1]
DR Ensembl; ENSRNOT00000116666; ENSRNOP00000081959; ENSRNOG00000054648. [Q9R1M3-2]
DR GeneID; 25223; -.
DR KEGG; rno:25223; -.
DR CTD; 7258; -.
DR RGD; 3912; Tspy1.
DR GeneTree; ENSGT00940000162417; -.
DR HOGENOM; CLU_051687_1_1_1; -.
DR InParanoid; Q9R1M3; -.
DR OMA; YFRNDIV; -.
DR OrthoDB; 1191764at2759; -.
DR PhylomeDB; Q9R1M3; -.
DR PRO; PR:Q9R1M3; -.
DR Proteomes; UP000002494; Unplaced.
DR Bgee; ENSRNOG00000054648; Expressed in testis and 2 other tissues.
DR Genevisible; Q9R1M3; RN.
DR GO; GO:0000785; C:chromatin; IBA:GO_Central.
DR GO; GO:0005737; C:cytoplasm; IDA:RGD.
DR GO; GO:0005634; C:nucleus; IBA:GO_Central.
DR GO; GO:0003682; F:chromatin binding; IBA:GO_Central.
DR GO; GO:0042393; F:histone binding; IDA:RGD.
DR GO; GO:0030154; P:cell differentiation; IEA:UniProtKB-KW.
DR GO; GO:0007506; P:gonadal mesoderm development; IEA:UniProtKB-KW.
DR GO; GO:0006334; P:nucleosome assembly; IEA:InterPro.
DR GO; GO:0007283; P:spermatogenesis; IEP:RGD.
DR InterPro; IPR037231; NAP-like_sf.
DR InterPro; IPR002164; NAP_family.
DR PANTHER; PTHR11875; PTHR11875; 1.
DR Pfam; PF00956; NAP; 1.
DR SUPFAM; SSF143113; SSF143113; 1.
PE 1: Evidence at protein level;
KW Alternative splicing; Cytoplasm; Developmental protein; Differentiation;
KW Gonadal differentiation; Nucleus; Phosphoprotein; Reference proteome;
KW Spermatogenesis.
FT CHAIN 1..334
FT /note="Testis-specific Y-encoded protein 1"
FT /id="PRO_0000185669"
FT REGION 27..46
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 96..146
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 96..124
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOD_RES 4
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:22673903"
FT VAR_SEQ 1..148
FT /note="Missing (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:10072602"
FT /id="VSP_008014"
FT VAR_SEQ 149..202
FT /note="MEELELLQLELSFVNARCSGAFARIKAKVAKMRRPHFDRRKTIIQGIPGFWA
FT KA -> MGYLQKHHFTESRLFVHPFVSSTFNSIAQFLDQRQ (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:10072602"
FT /id="VSP_008015"
SQ SEQUENCE 334 AA; 37998 MW; 2A49DD2AB52D924D CRC64;
MENSEEESVG VAPQEFRELV LLESSLLEGE PSVQAPEQSP GAPAGDNQMV KPLVICGPVE
DWVPLAEVLH HPDTCEEGDE ATMAELKVTE VVDVKNEGEE VKDQKQEGEQ DQQPELEKNP
EQACDSKDQQ GIQRLPLSGG GPAERRSKME ELELLQLELS FVNARCSGAF ARIKAKVAKM
RRPHFDRRKT IIQGIPGFWA KAMMNHPQMS SIISNQDEDL LSYMLSLEVE EYNPGLRMCR
MMFFFSENPY FRNDIVTKDY QLSIIGYKES DSSTIEWIGQ AEHGYANCMQ DTTRLTFFNW
LCAHKFPGSN RIAEIIMDDL WPNPLYYYPK EDHS
