TSP_BPT4
ID TSP_BPT4 Reviewed; 659 AA.
AC P13332; Q9T0U3;
DT 01-JAN-1990, integrated into UniProtKB/Swiss-Prot.
DT 23-JAN-2007, sequence version 5.
DT 29-SEP-2021, entry version 110.
DE RecName: Full=Tail sheath protein;
DE Short=TSP;
DE AltName: Full=Gene product 18;
DE Short=gp18;
GN Name=18;
OS Enterobacteria phage T4 (Bacteriophage T4).
OC Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes;
OC Caudovirales; Myoviridae; Tevenvirinae; Tequatrovirus.
OX NCBI_TaxID=10665;
OH NCBI_TaxID=562; Escherichia coli.
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND PARTIAL PROTEIN SEQUENCE.
RX PubMed=2964531; DOI=10.1128/jvi.62.4.1186-1193.1988;
RA Arisaka F., Nakako T., Takahashi H., Ishii S.;
RT "Nucleotide sequence of the tail sheath gene of bacteriophage T4 and amino
RT acid sequence of its product.";
RL J. Virol. 62:1186-1193(1988).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX PubMed=12626685; DOI=10.1128/mmbr.67.1.86-156.2003;
RA Miller E.S., Kutter E., Mosig G., Arisaka F., Kunisawa T., Ruger W.;
RT "Bacteriophage T4 genome.";
RL Microbiol. Mol. Biol. Rev. 67:86-156(2003).
RN [3]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 639-659.
RX PubMed=2963141; DOI=10.1128/jvi.62.3.882-886.1988;
RA Arisaka F., Ishimoto L., Kassavetis G., Kumazaki T., Ishii S.;
RT "Nucleotide sequence of the tail tube structural gene of bacteriophage
RT T4.";
RL J. Virol. 62:882-886(1988).
RN [4]
RP FUNCTION.
RX PubMed=14625682; DOI=10.1007/s00018-003-3072-1;
RA Leiman P.G., Kanamaru S., Mesyanzhinov V.V., Arisaka F., Rossmann M.G.;
RT "Structure and morphogenesis of bacteriophage T4.";
RL Cell. Mol. Life Sci. 60:2356-2370(2003).
RN [5]
RP SUBCELLULAR LOCATION AND SUBUNIT.
RX PubMed=15315755; DOI=10.1016/j.cell.2004.07.022;
RA Leiman P.G., Chipman P.R., Kostyuchenko V.A., Mesyanzhinov V.V.,
RA Rossmann M.G.;
RT "Three-dimensional rearrangement of proteins in the tail of bacteriophage
RT T4 on infection of its host.";
RL Cell 118:419-429(2004).
RN [6]
RP X-RAY CRYSTALLOGRAPHY (3.5 ANGSTROMS) OF 1-509.
RX PubMed=19229296; DOI=10.1038/emboj.2009.36;
RA Aksyuk A.A., Leiman P.G., Kurochkina L.P., Shneider M.M.,
RA Kostyuchenko V.A., Mesyanzhinov V.V., Rossmann M.G.;
RT "The tail sheath structure of bacteriophage T4: a molecular machine for
RT infecting bacteria.";
RL EMBO J. 28:821-829(2009).
CC -!- FUNCTION: Structural component of the bacteriophage tail which consists
CC of a contractile sheath, a tube and a baseplate. The central
CC cylindrical segment of the tail consists of a rigid tube, composed of
CC multiple copies of gp19, surrounded by the outer contractile sheath
CC assembled from gp18 subunits. A total of 138 copies of gp18 arranged
CC into 23 hexameric rings constitutes the sheath. During infection,
CC contraction of the sheath drives the central tube through the host
CC outer membrane, creating a channel for DNA ejection from the capsid
CC into the host cell. {ECO:0000269|PubMed:14625682}.
CC -!- SUBUNIT: Hexamer.
CC -!- SUBCELLULAR LOCATION: Virion {ECO:0000269|PubMed:15315755}.
CC -!- SIMILARITY: Belongs to the myoviridae tail sheath protein family.
CC {ECO:0000305}.
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; M19085; AAA32541.1; -; Genomic_DNA.
DR EMBL; AF158101; AAD42423.1; -; Genomic_DNA.
DR PIR; A60885; A60885.
DR PIR; JF0021; GKBPT4.
DR RefSeq; NP_049780.1; NC_000866.4.
DR PDB; 3FO8; X-ray; 1.80 A; D=83-365.
DR PDB; 3FOA; X-ray; 3.50 A; A/B/C/D=1-510.
DR PDB; 3FOH; EM; 15.00 A; A/B/C/D/E/F=1-510.
DR PDB; 3FOI; EM; 16.00 A; A/B/C/D/E/F=1-510.
DR PDB; 3J2M; EM; 15.00 A; U/V/W/X/Y/Z=1-659.
DR PDB; 3J2N; EM; 16.00 A; U/V/W/X/Y/Z=1-659.
DR PDBsum; 3FO8; -.
DR PDBsum; 3FOA; -.
DR PDBsum; 3FOH; -.
DR PDBsum; 3FOI; -.
DR PDBsum; 3J2M; -.
DR PDBsum; 3J2N; -.
DR SMR; P13332; -.
DR TCDB; 1.K.1.1.1; the gp27/5 t4-baseplate (t4-bp) family.
DR GeneID; 1258597; -.
DR KEGG; vg:1258597; -.
DR EvolutionaryTrace; P13332; -.
DR Proteomes; UP000009087; Genome.
DR GO; GO:0098027; C:virus tail, sheath; IDA:UniProtKB.
DR GO; GO:0099000; P:viral genome ejection through host cell envelope, contractile tail mechanism; IEA:UniProtKB-KW.
DR InterPro; IPR035326; Phage_sheath_beta.
DR InterPro; IPR035089; Phage_sheath_subtilisin.
DR InterPro; IPR020287; Tail_sheath_C.
DR Pfam; PF04984; Phage_sheath_1; 1.
DR Pfam; PF17482; Phage_sheath_1C; 1.
DR Pfam; PF17481; Phage_sheath_1N; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Direct protein sequencing; Reference proteome;
KW Viral contractile tail ejection system;
KW Viral genome ejection through host cell envelope;
KW Viral penetration into host cytoplasm; Viral tail protein;
KW Viral tail sheath protein; Virion; Virus entry into host cell.
FT INIT_MET 1
FT /note="Removed; by host"
FT CHAIN 2..659
FT /note="Tail sheath protein"
FT /id="PRO_0000165007"
FT CONFLICT 100
FT /note="D -> E (in Ref. 1; AAA32541)"
FT /evidence="ECO:0000305"
FT CONFLICT 148..151
FT /note="GKNY -> AKII (in Ref. 1; AAA32541)"
FT /evidence="ECO:0000305"
FT CONFLICT 301
FT /note="E -> G (in Ref. 1; AAA32541)"
FT /evidence="ECO:0000305"
FT CONFLICT 399
FT /note="A -> V (in Ref. 1; AAA32541)"
FT /evidence="ECO:0000305"
FT CONFLICT 454
FT /note="H -> Y (in Ref. 1; AAA32541)"
FT /evidence="ECO:0000305"
FT CONFLICT 595
FT /note="N -> I (in Ref. 1; AAA32541)"
FT /evidence="ECO:0000305"
FT STRAND 26..31
FT /evidence="ECO:0007829|PDB:3FOA"
FT STRAND 42..44
FT /evidence="ECO:0007829|PDB:3FOA"
FT HELIX 48..54
FT /evidence="ECO:0007829|PDB:3FOA"
FT TURN 59..61
FT /evidence="ECO:0007829|PDB:3FOA"
FT HELIX 62..69
FT /evidence="ECO:0007829|PDB:3FOA"
FT TURN 70..74
FT /evidence="ECO:0007829|PDB:3FOA"
FT STRAND 76..82
FT /evidence="ECO:0007829|PDB:3FOA"
FT TURN 86..88
FT /evidence="ECO:0007829|PDB:3FOA"
FT STRAND 93..95
FT /evidence="ECO:0007829|PDB:3FO8"
FT STRAND 99..105
FT /evidence="ECO:0007829|PDB:3FO8"
FT STRAND 115..120
FT /evidence="ECO:0007829|PDB:3FO8"
FT STRAND 123..134
FT /evidence="ECO:0007829|PDB:3FO8"
FT STRAND 140..144
FT /evidence="ECO:0007829|PDB:3FO8"
FT HELIX 148..157
FT /evidence="ECO:0007829|PDB:3FO8"
FT TURN 160..162
FT /evidence="ECO:0007829|PDB:3FO8"
FT STRAND 167..171
FT /evidence="ECO:0007829|PDB:3FO8"
FT STRAND 181..187
FT /evidence="ECO:0007829|PDB:3FO8"
FT STRAND 198..200
FT /evidence="ECO:0007829|PDB:3FOA"
FT HELIX 201..205
FT /evidence="ECO:0007829|PDB:3FO8"
FT HELIX 208..217
FT /evidence="ECO:0007829|PDB:3FO8"
FT STRAND 222..226
FT /evidence="ECO:0007829|PDB:3FO8"
FT HELIX 229..233
FT /evidence="ECO:0007829|PDB:3FO8"
FT STRAND 234..240
FT /evidence="ECO:0007829|PDB:3FO8"
FT HELIX 241..244
FT /evidence="ECO:0007829|PDB:3FO8"
FT HELIX 245..249
FT /evidence="ECO:0007829|PDB:3FO8"
FT STRAND 251..255
FT /evidence="ECO:0007829|PDB:3FO8"
FT STRAND 260..262
FT /evidence="ECO:0007829|PDB:3FO8"
FT HELIX 265..268
FT /evidence="ECO:0007829|PDB:3FO8"
FT STRAND 278..285
FT /evidence="ECO:0007829|PDB:3FO8"
FT STRAND 288..298
FT /evidence="ECO:0007829|PDB:3FO8"
FT HELIX 311..316
FT /evidence="ECO:0007829|PDB:3FO8"
FT STRAND 321..325
FT /evidence="ECO:0007829|PDB:3FO8"
FT STRAND 336..339
FT /evidence="ECO:0007829|PDB:3FO8"
FT HELIX 347..349
FT /evidence="ECO:0007829|PDB:3FO8"
FT HELIX 352..358
FT /evidence="ECO:0007829|PDB:3FO8"
FT HELIX 360..362
FT /evidence="ECO:0007829|PDB:3FOA"
FT TURN 365..367
FT /evidence="ECO:0007829|PDB:3FOA"
FT HELIX 376..378
FT /evidence="ECO:0007829|PDB:3FOA"
FT HELIX 383..400
FT /evidence="ECO:0007829|PDB:3FOA"
FT HELIX 411..414
FT /evidence="ECO:0007829|PDB:3FOA"
FT STRAND 415..417
FT /evidence="ECO:0007829|PDB:3FOA"
FT HELIX 419..429
FT /evidence="ECO:0007829|PDB:3FOA"
FT STRAND 446..449
FT /evidence="ECO:0007829|PDB:3FOA"
FT STRAND 453..459
FT /evidence="ECO:0007829|PDB:3FOA"
FT TURN 460..463
FT /evidence="ECO:0007829|PDB:3FOA"
FT STRAND 464..470
FT /evidence="ECO:0007829|PDB:3FOA"
FT HELIX 471..480
FT /evidence="ECO:0007829|PDB:3FOA"
FT STRAND 505..507
FT /evidence="ECO:0007829|PDB:3FOA"
SQ SEQUENCE 659 AA; 71331 MW; 5E5974F5BF799816 CRC64;
MTLLSPGIEL KETTVQSTVV NNSTGTAALA GKFQWGPAFQ IKQVTNEVDL VNTFGQPTAE
TADYFMSAMN FLQYGNDLRV VRAVDRDTAK NSSPIAGNID YTISTPGSNY AVGDKITVKY
VSDDIETEGK ITEVDADGKI KKINIPTGKN YAKAKEVGEY PTLGSNWTAE ISSSSSGLAA
VITLGKIITD SGILLAEIEN AEAAMTAVDF QANLKKYGIP GVVALYPGEL GDKIEIEIVS
KADYAKGASA LLPIYPGGGT RASTAKAVFG YGPQTDSQYA IIVRRNDAIV QSVVLSTKRG
EKDIYDSNIY IDDFFAKGGS EYIFATAQNW PEGFSGILTL SGGLSSNAEV TAGDLMEAWD
FFADRESVDV QLFIAGSCAG ESLETASTVQ KHVVSIGDAR QDCLVLCSPP RETVVGIPVT
RAVDNLVNWR TAAGSYTDNN FNISSTYAAI DGNHKYQYDK YNDVNRWVPL AADIAGLCAR
TDNVSQTWMS PAGYNRGQIL NVIKLAIETR QAQRDRLYQE AINPVTGTGG DGYVLYGDKT
ATSVPSPFDR INVRRLFNML KTNIGRSSKY RLFELNNAFT RSSFRTETAQ YLQGNKALGG
IYEYRVVCDT TNNTPSVIDR NEFVATFYIQ PARSINYITL NFVATATGAD FDELTGLAG
