TSR1_DROME
ID TSR1_DROME Reviewed; 814 AA.
AC Q9VP47; B5RIG8; Q86PD4; Q95TR7;
DT 13-NOV-2007, integrated into UniProtKB/Swiss-Prot.
DT 01-MAY-2000, sequence version 1.
DT 03-AUG-2022, entry version 129.
DE RecName: Full=Pre-rRNA-processing protein TSR1 homolog;
DE AltName: Full=Tsr1 ribosome assembly factor {ECO:0000312|FlyBase:FBgn0037073};
GN Name=Tsr1 {ECO:0000312|FlyBase:FBgn0037073};
GN ORFNames=CG7338 {ECO:0000312|FlyBase:FBgn0037073};
OS Drosophila melanogaster (Fruit fly).
OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea;
OC Drosophilidae; Drosophila; Sophophora.
OX NCBI_TaxID=7227;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Berkeley;
RX PubMed=10731132; DOI=10.1126/science.287.5461.2185;
RA Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D.,
RA Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F.,
RA George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N.,
RA Sutton G.G., Wortman J.R., Yandell M.D., Zhang Q., Chen L.X., Brandon R.C.,
RA Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C.,
RA Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A.,
RA An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A.,
RA Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V.,
RA Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J.,
RA Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E.,
RA Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B.,
RA Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I.,
RA Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C.,
RA Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S.,
RA Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M.,
RA Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M.,
RA Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D.,
RA Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F.,
RA Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D.,
RA Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A.,
RA Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C.,
RA McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C.,
RA Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L.,
RA Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R.,
RA Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V.,
RA Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F.,
RA Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J.,
RA Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R.,
RA Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y.,
RA Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T.,
RA Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S.,
RA Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W.,
RA Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M.,
RA Venter J.C.;
RT "The genome sequence of Drosophila melanogaster.";
RL Science 287:2185-2195(2000).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=Berkeley;
RX PubMed=12537572; DOI=10.1186/gb-2002-3-12-research0083;
RA Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S.,
RA Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E.,
RA Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P.,
RA Bettencourt B.R., Celniker S.E., de Grey A.D.N.J., Drysdale R.A.,
RA Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M.,
RA Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E.;
RT "Annotation of the Drosophila melanogaster euchromatic genome: a systematic
RT review.";
RL Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=Berkeley; TISSUE=Embryo;
RA Stapleton M., Brokstein P., Hong L., Agbayani A., Booth B., Carlson J.W.,
RA Champe M., Chavez C., Dorsett V., Dresnek D., Farfan D., Frise E.,
RA George R.A., Gonzalez M., Guarin H., Kronmiller B., Li P.W., Liao G.,
RA Miranda A., Mungall C.J., Nunoo J., Pacleb J.M., Paragas V., Park S.,
RA Patel S., Phouanenavong S., Wan K.H., Yu C., Lewis S.E., Rubin G.M.,
RA Celniker S.E.;
RL Submitted (SEP-2008) to the EMBL/GenBank/DDBJ databases.
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 189-814.
RC STRAIN=Berkeley; TISSUE=Embryo;
RX PubMed=12537569; DOI=10.1186/gb-2002-3-12-research0080;
RA Stapleton M., Carlson J.W., Brokstein P., Yu C., Champe M., George R.A.,
RA Guarin H., Kronmiller B., Pacleb J.M., Park S., Wan K.H., Rubin G.M.,
RA Celniker S.E.;
RT "A Drosophila full-length cDNA resource.";
RL Genome Biol. 3:RESEARCH0080.1-RESEARCH0080.8(2002).
RN [5]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-444, AND IDENTIFICATION BY
RP MASS SPECTROMETRY.
RC TISSUE=Embryo;
RX PubMed=18327897; DOI=10.1021/pr700696a;
RA Zhai B., Villen J., Beausoleil S.A., Mintseris J., Gygi S.P.;
RT "Phosphoproteome analysis of Drosophila melanogaster embryos.";
RL J. Proteome Res. 7:1675-1682(2008).
CC -!- FUNCTION: Required during maturation of the 40S ribosomal subunit in
CC the nucleolus. {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Nucleus, nucleolus {ECO:0000250}.
CC -!- SIMILARITY: Belongs to the TRAFAC class translation factor GTPase
CC superfamily. Bms1-like GTPase family. TSR1 subfamily. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAL13820.1; Type=Erroneous initiation; Evidence={ECO:0000305};
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DR EMBL; AE014296; AAF51709.1; -; Genomic_DNA.
DR EMBL; BT003192; AAO24947.1; -; mRNA.
DR EMBL; BT044092; ACH92157.1; -; mRNA.
DR EMBL; AY058591; AAL13820.1; ALT_INIT; mRNA.
DR RefSeq; NP_649304.1; NM_141047.4.
DR AlphaFoldDB; Q9VP47; -.
DR SMR; Q9VP47; -.
DR BioGRID; 65608; 4.
DR IntAct; Q9VP47; 2.
DR STRING; 7227.FBpp0077998; -.
DR iPTMnet; Q9VP47; -.
DR PaxDb; Q9VP47; -.
DR PRIDE; Q9VP47; -.
DR DNASU; 40360; -.
DR EnsemblMetazoa; FBtr0078342; FBpp0077998; FBgn0037073.
DR GeneID; 40360; -.
DR KEGG; dme:Dmel_CG7338; -.
DR UCSC; CG7338-RA; d. melanogaster.
DR CTD; 55720; -.
DR FlyBase; FBgn0037073; Tsr1.
DR VEuPathDB; VectorBase:FBgn0037073; -.
DR eggNOG; KOG1980; Eukaryota.
DR GeneTree; ENSGT00940000153195; -.
DR HOGENOM; CLU_009858_1_0_1; -.
DR InParanoid; Q9VP47; -.
DR OMA; KTPHQQV; -.
DR OrthoDB; 186926at2759; -.
DR PhylomeDB; Q9VP47; -.
DR BioGRID-ORCS; 40360; 1 hit in 1 CRISPR screen.
DR GenomeRNAi; 40360; -.
DR PRO; PR:Q9VP47; -.
DR Proteomes; UP000000803; Chromosome 3L.
DR Bgee; FBgn0037073; Expressed in egg chamber and 25 other tissues.
DR Genevisible; Q9VP47; DM.
DR GO; GO:0005730; C:nucleolus; ISS:UniProtKB.
DR GO; GO:0030688; C:preribosome, small subunit precursor; IBA:GO_Central.
DR GO; GO:0005525; F:GTP binding; IBA:GO_Central.
DR GO; GO:0003924; F:GTPase activity; IBA:GO_Central.
DR GO; GO:0034511; F:U3 snoRNA binding; IBA:GO_Central.
DR GO; GO:0000479; P:endonucleolytic cleavage of tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA); IBA:GO_Central.
DR GO; GO:0000462; P:maturation of SSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA); IBA:GO_Central.
DR InterPro; IPR012948; AARP2CN.
DR InterPro; IPR039761; Bms1/Tsr1.
DR InterPro; IPR007034; BMS1_TSR1_C.
DR InterPro; IPR030387; G_Bms1/Tsr1_dom.
DR PANTHER; PTHR12858; PTHR12858; 1.
DR Pfam; PF08142; AARP2CN; 1.
DR Pfam; PF04950; RIBIOP_C; 1.
DR SMART; SM00785; AARP2CN; 1.
DR SMART; SM01362; DUF663; 1.
DR PROSITE; PS51714; G_BMS1; 1.
PE 1: Evidence at protein level;
KW Nucleus; Phosphoprotein; Reference proteome; Ribosome biogenesis.
FT CHAIN 1..814
FT /note="Pre-rRNA-processing protein TSR1 homolog"
FT /id="PRO_0000311280"
FT DOMAIN 84..249
FT /note="Bms1-type G"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01051"
FT REGION 1..67
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 316..357
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 392..448
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 317..341
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 394..423
FT /note="Acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 424..447
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOD_RES 444
FT /note="Phosphothreonine"
FT /evidence="ECO:0000269|PubMed:18327897"
FT CONFLICT 570
FT /note="E -> V (in Ref. 3; AAO24947)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 814 AA; 93717 MW; 3C9299F0F65556A3 CRC64;
MADHAFHRPG PLKQANKAHK TGRHRSKGAI DNAQKGKIGL RPISHKHKQQ QRKEQRRNQM
NQLRKNKREE VLEQKRKLGG QNTAPFLVCL LPMHEQIDPM SALEILKSCD SELVVENSPS
GIVYINLPRF KQRFAFVTPP VGRGNELIAL DYLKVCDTTL LLTTAAFGDD EIFDRWGQRI
FNMMSAQGIP TPVVALMDLE SINPKRRPAA KQAAQKVISK LLPEEKIMQL DTASEALNVM
RRIGGQKKRI LHNVANRPHL FGDVVEFKPG SDPSDDLGTL EVTGFLRGQS LNVNGLVHIP
GLGDFQLSQV VAPPDPYKLD KSRDGENSEV RLLDRSDPSK RTSLQSENIP DPMDAEQTWP
TEDEIAASQA ETKKMKLVKR VPKGYSEYQA AWIPDVEEVE DPDGKDDDDM SEDDDDDKED
DNEDFMSCDN KSFEDEYEKR DSDTEEFQDT VSVASEAAIN DEKYDQQMDF QEERETLKKL
QQARTDQLWP DEIDTPLDVP ARERFQKYRG LESFRTSPWD AKENLPADYA RIYQFQNFDR
TKRRILNEAK EFEGVLPGLY VTLYVINVPE SRWNAFKSAQ LMDNIIVYGM LPHEHQMCVM
NVVLQRMPDS EVPLKSKEQL IIQCGYRRFV VNPIYSQHTN GDKHKFERYF RPYETVCATF
YAPIQFPPAP VLAFKVNPDS TLALVARGRL LSCNPDRIVL KRVVLSGHPM RINRKSASIR
YMFFYKEDVE YFKPVKLRTK CGRLGHIKES LGTHGHMKCY FDGQLRSYDT AFMYLYKRVF
PKWTYEECLV RTAEHERQHA SANRRSSQQV AMEE
