TSR1_HUMAN
ID TSR1_HUMAN Reviewed; 804 AA.
AC Q2NL82; Q8WUY5; Q9NVT0; Q9P2E6;
DT 13-NOV-2007, integrated into UniProtKB/Swiss-Prot.
DT 07-FEB-2006, sequence version 1.
DT 03-AUG-2022, entry version 145.
DE RecName: Full=Pre-rRNA-processing protein TSR1 homolog;
GN Name=TSR1; Synonyms=KIAA1401;
OS Homo sapiens (Human).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae;
OC Homo.
OX NCBI_TaxID=9606;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC TISSUE=Brain;
RX PubMed=10718198; DOI=10.1093/dnares/7.1.65;
RA Nagase T., Kikuno R., Ishikawa K., Hirosawa M., Ohara O.;
RT "Prediction of the coding sequences of unidentified human genes. XVI. The
RT complete sequences of 150 new cDNA clones from brain which code for large
RT proteins in vitro.";
RL DNA Res. 7:65-73(2000).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RA Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M.,
RA Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J.,
RA Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S.,
RA Turner R., Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H.,
RA Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K.,
RA Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D.,
RA Hunkapiller M.W., Myers E.W., Venter J.C.;
RL Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases.
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA], AND VARIANT GLY-386.
RC TISSUE=Brain, and Lymph;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [4]
RP PROTEIN SEQUENCE OF 79-106; 140-151; 220-234; 251-273; 480-492; 518-537;
RP 625-637 AND 721-732, AND IDENTIFICATION BY MASS SPECTROMETRY.
RC TISSUE=Hepatoma;
RA Bienvenut W.V., Boldt K., von Kriegsheim A.F., Kolch W.;
RL Submitted (JUL-2007) to UniProtKB.
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 135-804.
RC TISSUE=Teratocarcinoma;
RX PubMed=14702039; DOI=10.1038/ng1285;
RA Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R.,
RA Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H.,
RA Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.,
RA Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K.,
RA Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H.,
RA Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M.,
RA Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K.,
RA Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T.,
RA Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M.,
RA Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S.,
RA Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H.,
RA Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K.,
RA Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N.,
RA Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S.,
RA Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O.,
RA Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H.,
RA Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B.,
RA Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y.,
RA Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K.,
RA Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T.,
RA Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T.,
RA Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y.,
RA Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H.,
RA Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y.,
RA Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H.,
RA Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O.,
RA Isogai T., Sugano S.;
RT "Complete sequencing and characterization of 21,243 full-length human
RT cDNAs.";
RL Nat. Genet. 36:40-45(2004).
RN [6]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RX PubMed=21269460; DOI=10.1186/1752-0509-5-17;
RA Burkard T.R., Planyavsky M., Kaupe I., Breitwieser F.P., Buerckstuemmer T.,
RA Bennett K.L., Superti-Furga G., Colinge J.;
RT "Initial characterization of the human central proteome.";
RL BMC Syst. Biol. 5:17-17(2011).
CC -!- FUNCTION: Required during maturation of the 40S ribosomal subunit in
CC the nucleolus. {ECO:0000250}.
CC -!- INTERACTION:
CC Q2NL82; Q9UHD4: CIDEB; NbExp=3; IntAct=EBI-358058, EBI-7062247;
CC Q2NL82; Q6NTF9-3: RHBDD2; NbExp=3; IntAct=EBI-358058, EBI-17589229;
CC -!- SUBCELLULAR LOCATION: Nucleus, nucleolus {ECO:0000250}.
CC -!- SIMILARITY: Belongs to the TRAFAC class translation factor GTPase
CC superfamily. Bms1-like GTPase family. TSR1 subfamily. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=BAA91667.1; Type=Erroneous initiation; Evidence={ECO:0000305};
CC Sequence=BAA92639.1; Type=Erroneous initiation; Evidence={ECO:0000305};
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DR EMBL; AB037822; BAA92639.1; ALT_INIT; mRNA.
DR EMBL; CH471108; EAW90552.1; -; Genomic_DNA.
DR EMBL; BC019090; AAH19090.2; -; mRNA.
DR EMBL; BC110851; AAI10852.1; -; mRNA.
DR EMBL; BC126110; AAI26111.1; -; mRNA.
DR EMBL; AK001396; BAA91667.1; ALT_INIT; mRNA.
DR CCDS; CCDS32525.1; -.
DR RefSeq; NP_060598.3; NM_018128.4.
DR PDB; 6G18; EM; 3.60 A; u=1-804.
DR PDB; 6G4S; EM; 4.00 A; u=1-804.
DR PDB; 6G4W; EM; 4.50 A; u=1-804.
DR PDB; 6G51; EM; 4.10 A; u=1-804.
DR PDB; 6G53; EM; 4.50 A; u=1-804.
DR PDB; 6ZMT; EM; 3.00 A; u=1-804.
DR PDB; 6ZN5; EM; 3.20 A; u=1-804.
DR PDBsum; 6G18; -.
DR PDBsum; 6G4S; -.
DR PDBsum; 6G4W; -.
DR PDBsum; 6G51; -.
DR PDBsum; 6G53; -.
DR PDBsum; 6ZMT; -.
DR PDBsum; 6ZN5; -.
DR AlphaFoldDB; Q2NL82; -.
DR SMR; Q2NL82; -.
DR BioGRID; 120842; 210.
DR IntAct; Q2NL82; 68.
DR MINT; Q2NL82; -.
DR STRING; 9606.ENSP00000301364; -.
DR GlyGen; Q2NL82; 1 site, 1 O-linked glycan (1 site).
DR iPTMnet; Q2NL82; -.
DR MetOSite; Q2NL82; -.
DR PhosphoSitePlus; Q2NL82; -.
DR BioMuta; TSR1; -.
DR DMDM; 121948971; -.
DR EPD; Q2NL82; -.
DR jPOST; Q2NL82; -.
DR MassIVE; Q2NL82; -.
DR MaxQB; Q2NL82; -.
DR PaxDb; Q2NL82; -.
DR PeptideAtlas; Q2NL82; -.
DR PRIDE; Q2NL82; -.
DR ProteomicsDB; 61424; -.
DR Antibodypedia; 10721; 114 antibodies from 23 providers.
DR DNASU; 55720; -.
DR Ensembl; ENST00000301364.10; ENSP00000301364.4; ENSG00000167721.11.
DR GeneID; 55720; -.
DR KEGG; hsa:55720; -.
DR MANE-Select; ENST00000301364.10; ENSP00000301364.4; NM_018128.5; NP_060598.3.
DR UCSC; uc002fuj.4; human.
DR CTD; 55720; -.
DR DisGeNET; 55720; -.
DR GeneCards; TSR1; -.
DR HGNC; HGNC:25542; TSR1.
DR HPA; ENSG00000167721; Low tissue specificity.
DR MIM; 611214; gene.
DR neXtProt; NX_Q2NL82; -.
DR OpenTargets; ENSG00000167721; -.
DR PharmGKB; PA142670691; -.
DR VEuPathDB; HostDB:ENSG00000167721; -.
DR eggNOG; KOG1980; Eukaryota.
DR GeneTree; ENSGT00940000153195; -.
DR HOGENOM; CLU_009858_1_0_1; -.
DR InParanoid; Q2NL82; -.
DR OMA; KTPHQQV; -.
DR OrthoDB; 186926at2759; -.
DR PhylomeDB; Q2NL82; -.
DR TreeFam; TF105717; -.
DR PathwayCommons; Q2NL82; -.
DR Reactome; R-HSA-6791226; Major pathway of rRNA processing in the nucleolus and cytosol.
DR SignaLink; Q2NL82; -.
DR BioGRID-ORCS; 55720; 711 hits in 1087 CRISPR screens.
DR ChiTaRS; TSR1; human.
DR GenomeRNAi; 55720; -.
DR Pharos; Q2NL82; Tbio.
DR PRO; PR:Q2NL82; -.
DR Proteomes; UP000005640; Chromosome 17.
DR RNAct; Q2NL82; protein.
DR Bgee; ENSG00000167721; Expressed in cervix squamous epithelium and 225 other tissues.
DR ExpressionAtlas; Q2NL82; baseline and differential.
DR Genevisible; Q2NL82; HS.
DR GO; GO:0005829; C:cytosol; TAS:Reactome.
DR GO; GO:0005730; C:nucleolus; ISS:UniProtKB.
DR GO; GO:0005654; C:nucleoplasm; TAS:Reactome.
DR GO; GO:0030688; C:preribosome, small subunit precursor; IBA:GO_Central.
DR GO; GO:0005525; F:GTP binding; IBA:GO_Central.
DR GO; GO:0003924; F:GTPase activity; IBA:GO_Central.
DR GO; GO:0003723; F:RNA binding; HDA:UniProtKB.
DR GO; GO:0034511; F:U3 snoRNA binding; IBA:GO_Central.
DR GO; GO:0000479; P:endonucleolytic cleavage of tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA); IBA:GO_Central.
DR GO; GO:0000462; P:maturation of SSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA); IBA:GO_Central.
DR InterPro; IPR012948; AARP2CN.
DR InterPro; IPR039761; Bms1/Tsr1.
DR InterPro; IPR007034; BMS1_TSR1_C.
DR InterPro; IPR030387; G_Bms1/Tsr1_dom.
DR PANTHER; PTHR12858; PTHR12858; 1.
DR Pfam; PF08142; AARP2CN; 1.
DR Pfam; PF04950; RIBIOP_C; 1.
DR SMART; SM00785; AARP2CN; 1.
DR SMART; SM01362; DUF663; 1.
DR PROSITE; PS51714; G_BMS1; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Direct protein sequencing; Nucleus; Reference proteome;
KW Ribosome biogenesis.
FT CHAIN 1..804
FT /note="Pre-rRNA-processing protein TSR1 homolog"
FT /id="PRO_0000311274"
FT DOMAIN 81..242
FT /note="Bms1-type G"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01051"
FT REGION 1..67
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 24..56
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT VARIANT 386
FT /note="S -> G (in dbSNP:rs2281726)"
FT /evidence="ECO:0000269|PubMed:15489334"
FT /id="VAR_037211"
FT VARIANT 719
FT /note="N -> S (in dbSNP:rs2273983)"
FT /id="VAR_037212"
FT VARIANT 727
FT /note="K -> Q (in dbSNP:rs35019711)"
FT /id="VAR_037213"
FT VARIANT 750
FT /note="H -> Q (in dbSNP:rs35343613)"
FT /id="VAR_037214"
FT CONFLICT 350
FT /note="G -> C (in Ref. 5; BAA91667)"
FT /evidence="ECO:0000305"
FT CONFLICT 737
FT /note="R -> W (in Ref. 1; BAA92639)"
FT /evidence="ECO:0000305"
FT HELIX 50..71
FT /evidence="ECO:0007829|PDB:6ZMT"
FT STRAND 74..80
FT /evidence="ECO:0007829|PDB:6ZMT"
FT STRAND 83..91
FT /evidence="ECO:0007829|PDB:6ZMT"
FT HELIX 96..103
FT /evidence="ECO:0007829|PDB:6ZMT"
FT STRAND 110..112
FT /evidence="ECO:0007829|PDB:6ZMT"
FT STRAND 117..119
FT /evidence="ECO:0007829|PDB:6ZN5"
FT STRAND 122..126
FT /evidence="ECO:0007829|PDB:6ZMT"
FT TURN 127..130
FT /evidence="ECO:0007829|PDB:6ZMT"
FT STRAND 131..137
FT /evidence="ECO:0007829|PDB:6ZMT"
FT HELIX 143..149
FT /evidence="ECO:0007829|PDB:6ZMT"
FT TURN 150..152
FT /evidence="ECO:0007829|PDB:6ZMT"
FT STRAND 154..160
FT /evidence="ECO:0007829|PDB:6ZMT"
FT STRAND 162..165
FT /evidence="ECO:0007829|PDB:6ZMT"
FT HELIX 168..180
FT /evidence="ECO:0007829|PDB:6ZMT"
FT STRAND 184..186
FT /evidence="ECO:0007829|PDB:6ZMT"
FT STRAND 188..190
FT /evidence="ECO:0007829|PDB:6ZN5"
FT STRAND 193..195
FT /evidence="ECO:0007829|PDB:6ZMT"
FT TURN 197..202
FT /evidence="ECO:0007829|PDB:6ZMT"
FT HELIX 203..211
FT /evidence="ECO:0007829|PDB:6ZMT"
FT TURN 212..214
FT /evidence="ECO:0007829|PDB:6ZMT"
FT STRAND 215..217
FT /evidence="ECO:0007829|PDB:6ZMT"
FT HELIX 226..237
FT /evidence="ECO:0007829|PDB:6ZMT"
FT HELIX 245..249
FT /evidence="ECO:0007829|PDB:6ZMT"
FT STRAND 256..264
FT /evidence="ECO:0007829|PDB:6ZMT"
FT TURN 265..268
FT /evidence="ECO:0007829|PDB:6ZMT"
FT STRAND 269..278
FT /evidence="ECO:0007829|PDB:6ZMT"
FT STRAND 285..287
FT /evidence="ECO:0007829|PDB:6ZMT"
FT STRAND 289..291
FT /evidence="ECO:0007829|PDB:6ZMT"
FT STRAND 299..304
FT /evidence="ECO:0007829|PDB:6ZMT"
FT STRAND 343..347
FT /evidence="ECO:0007829|PDB:6ZN5"
FT TURN 350..352
FT /evidence="ECO:0007829|PDB:6ZMT"
FT HELIX 465..480
FT /evidence="ECO:0007829|PDB:6ZMT"
FT TURN 481..483
FT /evidence="ECO:0007829|PDB:6ZMT"
FT STRAND 484..487
FT /evidence="ECO:0007829|PDB:6ZMT"
FT STRAND 492..494
FT /evidence="ECO:0007829|PDB:6ZMT"
FT HELIX 496..499
FT /evidence="ECO:0007829|PDB:6ZMT"
FT TURN 500..502
FT /evidence="ECO:0007829|PDB:6ZMT"
FT TURN 509..511
FT /evidence="ECO:0007829|PDB:6ZMT"
FT HELIX 522..525
FT /evidence="ECO:0007829|PDB:6ZMT"
FT HELIX 533..544
FT /evidence="ECO:0007829|PDB:6ZMT"
FT STRAND 545..547
FT /evidence="ECO:0007829|PDB:6ZMT"
FT STRAND 550..552
FT /evidence="ECO:0007829|PDB:6ZN5"
FT STRAND 557..563
FT /evidence="ECO:0007829|PDB:6ZMT"
FT STRAND 565..567
FT /evidence="ECO:0007829|PDB:6ZMT"
FT HELIX 568..575
FT /evidence="ECO:0007829|PDB:6ZMT"
FT STRAND 581..583
FT /evidence="ECO:0007829|PDB:6ZMT"
FT STRAND 589..598
FT /evidence="ECO:0007829|PDB:6ZMT"
FT STRAND 614..620
FT /evidence="ECO:0007829|PDB:6ZMT"
FT STRAND 623..625
FT /evidence="ECO:0007829|PDB:6ZMT"
FT STRAND 634..640
FT /evidence="ECO:0007829|PDB:6ZMT"
FT STRAND 646..648
FT /evidence="ECO:0007829|PDB:6ZMT"
FT STRAND 650..657
FT /evidence="ECO:0007829|PDB:6ZMT"
FT STRAND 664..672
FT /evidence="ECO:0007829|PDB:6ZMT"
FT STRAND 675..684
FT /evidence="ECO:0007829|PDB:6ZMT"
FT STRAND 691..707
FT /evidence="ECO:0007829|PDB:6ZMT"
FT STRAND 710..716
FT /evidence="ECO:0007829|PDB:6ZMT"
FT STRAND 718..720
FT /evidence="ECO:0007829|PDB:6ZN5"
FT HELIX 721..725
FT /evidence="ECO:0007829|PDB:6ZMT"
FT STRAND 726..729
FT /evidence="ECO:0007829|PDB:6ZN5"
FT STRAND 731..733
FT /evidence="ECO:0007829|PDB:6ZMT"
FT STRAND 738..744
FT /evidence="ECO:0007829|PDB:6ZMT"
FT STRAND 746..749
FT /evidence="ECO:0007829|PDB:6ZMT"
FT STRAND 751..757
FT /evidence="ECO:0007829|PDB:6ZMT"
FT STRAND 765..772
FT /evidence="ECO:0007829|PDB:6ZMT"
SQ SEQUENCE 804 AA; 91810 MW; EDDBE6AD04E7F3A7 CRC64;
MAAHRPGPLK QQNKAHKGGR HRGRGSAQRD GKGRLALKTL SKKVRKELSR VDQRHRASQL
RKQKKEAVLA EKRQLGGKDG PPHQVLVVPL HSRISLPEAM QLLQDRDTGT VHLNELGNTQ
NFMLLCPRLK HRWFFTSARP GDLHVVLDMA KVADTILFLL DPLEGWDSTG DYCLSCLFAQ
GLPTYTLAVQ GISGLPLKKQ IDTRKKLSKA VEKRFPHDKL LLLDTQQEAG MLLRQLANQK
QQHLAFRDRR AYLFAHAVDF VPSEENNLVG TLKISGYVRG QTLNVNRLLH IVGYGDFQMK
QIDAPGDPFP LNPRGIKPQK DPDMAMEICA TDAVDDMEEG LKVLMKADPG RQESLQAEVI
PDPMEGEQTW PTEEELSEAK DFLKESSKVV KKVPKGTSSY QAEWILDGGS QSGGEGDEYE
YDDMEHEDFM EEESQDESSE EEEEYETMTI GESVHDDLYD KKVDEEAEAK MLEKYKQERL
EEMFPDEVDT PRDVAARIRF QKYRGLKSFR TSPWDPKENL PQDYARIFQF QNFTNTRKSI
FKEVEEKEVE GAEVGWYVTL HVSEVPVSVV ECFRQGTPLI AFSLLPHEQK MSVLNMVVRR
DPGNTEPVKA KEELIFHCGF RRFRASPLFS QHTAADKHKL QRFLTADMAL VATVYAPITF
PPASVLLFKQ KSNGMHSLIA TGHLMSVDPD RMVIKRVVLS GHPFKIFTKM AVVRYMFFNR
EDVLWFKPVE LRTKWGRRGH IKEPLGTHGH MKCSFDGKLK SQDTVLMNLY KRVFPKWTYD
PYVPEPVPWL KSEISSTVPQ GGME
