TSR1_MOUSE
ID TSR1_MOUSE Reviewed; 803 AA.
AC Q5SWD9; Q3TMW6; Q3TRK6; Q3U4T2; Q3UYU4; Q3V3X8; Q6ZPR9; Q8K2F5;
DT 13-NOV-2007, integrated into UniProtKB/Swiss-Prot.
DT 21-DEC-2004, sequence version 1.
DT 03-AUG-2022, entry version 105.
DE RecName: Full=Pre-rRNA-processing protein TSR1 homolog;
GN Name=Tsr1; Synonyms=Kiaa1401;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 3), AND NUCLEOTIDE
RP SEQUENCE [LARGE SCALE MRNA] OF 1-535 (ISOFORM 2).
RC STRAIN=C57BL/6J, and NOD; TISSUE=Pituitary, Testis, Thymus, and Vagina;
RX PubMed=16141072; DOI=10.1126/science.1112014;
RA Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N.,
RA Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K.,
RA Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.,
RA Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R.,
RA Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T.,
RA Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A.,
RA Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B.,
RA Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M.,
RA Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S.,
RA Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E.,
RA Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D.,
RA Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M.,
RA Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H.,
RA Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V.,
RA Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S.,
RA Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H.,
RA Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N.,
RA Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F.,
RA Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G.,
RA Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z.,
RA Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C.,
RA Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y.,
RA Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S.,
RA Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K.,
RA Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R.,
RA van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H.,
RA Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M.,
RA Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C.,
RA Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S.,
RA Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K.,
RA Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M.,
RA Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C.,
RA Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A.,
RA Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.;
RT "The transcriptional landscape of the mammalian genome.";
RL Science 309:1559-1563(2005).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=C57BL/6J;
RX PubMed=19468303; DOI=10.1371/journal.pbio.1000112;
RA Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X.,
RA Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y.,
RA Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S.,
RA Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R.,
RA Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K.,
RA Eichler E.E., Ponting C.P.;
RT "Lineage-specific biology revealed by a finished genome assembly of the
RT mouse.";
RL PLoS Biol. 7:E1000112-E1000112(2009).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
RC STRAIN=Czech II; TISSUE=Mammary tumor;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 4-803 (ISOFORM 1).
RX PubMed=14621295; DOI=10.1093/dnares/10.4.167;
RA Okazaki N., Kikuno R., Ohara R., Inamoto S., Koseki H., Hiraoka S.,
RA Saga Y., Nagase T., Ohara O., Koga H.;
RT "Prediction of the coding sequences of mouse homologues of KIAA gene: III.
RT The complete nucleotide sequences of 500 mouse KIAA-homologous cDNAs
RT identified by screening of terminal sequences of cDNA clones randomly
RT sampled from size-fractionated libraries.";
RL DNA Res. 10:167-180(2003).
RN [5]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Liver, Lung, Pancreas, Spleen, and Testis;
RX PubMed=21183079; DOI=10.1016/j.cell.2010.12.001;
RA Huttlin E.L., Jedrychowski M.P., Elias J.E., Goswami T., Rad R.,
RA Beausoleil S.A., Villen J., Haas W., Sowa M.E., Gygi S.P.;
RT "A tissue-specific atlas of mouse protein phosphorylation and expression.";
RL Cell 143:1174-1189(2010).
CC -!- FUNCTION: Required during maturation of the 40S ribosomal subunit in
CC the nucleolus. {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Nucleus, nucleolus {ECO:0000250}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=3;
CC Name=1;
CC IsoId=Q5SWD9-1; Sequence=Displayed;
CC Name=2;
CC IsoId=Q5SWD9-2; Sequence=VSP_029504;
CC Name=3;
CC IsoId=Q5SWD9-3; Sequence=VSP_029505, VSP_029506;
CC -!- SIMILARITY: Belongs to the TRAFAC class translation factor GTPase
CC superfamily. Bms1-like GTPase family. TSR1 subfamily. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAH31531.1; Type=Erroneous initiation; Note=Truncated N-terminus.; Evidence={ECO:0000305};
CC Sequence=AAH31531.1; Type=Frameshift; Evidence={ECO:0000305};
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DR EMBL; AK030545; BAE20453.1; -; mRNA.
DR EMBL; AK134365; BAE22117.1; -; mRNA.
DR EMBL; AK154063; BAE32349.1; -; mRNA.
DR EMBL; AK162686; BAE37023.1; -; mRNA.
DR EMBL; AK165662; BAE38324.1; -; mRNA.
DR EMBL; AL604066; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; BC031531; AAH31531.1; ALT_SEQ; mRNA.
DR EMBL; AK129350; BAC98160.1; -; mRNA.
DR CCDS; CCDS36225.1; -. [Q5SWD9-1]
DR RefSeq; NP_796299.2; NM_177325.3. [Q5SWD9-1]
DR AlphaFoldDB; Q5SWD9; -.
DR SMR; Q5SWD9; -.
DR BioGRID; 222666; 4.
DR IntAct; Q5SWD9; 1.
DR STRING; 10090.ENSMUSP00000039027; -.
DR iPTMnet; Q5SWD9; -.
DR PhosphoSitePlus; Q5SWD9; -.
DR SwissPalm; Q5SWD9; -.
DR EPD; Q5SWD9; -.
DR MaxQB; Q5SWD9; -.
DR PaxDb; Q5SWD9; -.
DR PeptideAtlas; Q5SWD9; -.
DR PRIDE; Q5SWD9; -.
DR ProteomicsDB; 300139; -. [Q5SWD9-1]
DR ProteomicsDB; 300140; -. [Q5SWD9-2]
DR ProteomicsDB; 300141; -. [Q5SWD9-3]
DR Antibodypedia; 10721; 114 antibodies from 23 providers.
DR Ensembl; ENSMUST00000045807; ENSMUSP00000039027; ENSMUSG00000038335. [Q5SWD9-1]
DR GeneID; 104662; -.
DR KEGG; mmu:104662; -.
DR UCSC; uc007kcm.1; mouse. [Q5SWD9-3]
DR UCSC; uc007kcn.1; mouse. [Q5SWD9-1]
DR CTD; 55720; -.
DR MGI; MGI:2144566; Tsr1.
DR VEuPathDB; HostDB:ENSMUSG00000038335; -.
DR eggNOG; KOG1980; Eukaryota.
DR GeneTree; ENSGT00940000153195; -.
DR HOGENOM; CLU_009858_1_0_1; -.
DR InParanoid; Q5SWD9; -.
DR OMA; KTPHQQV; -.
DR OrthoDB; 186926at2759; -.
DR PhylomeDB; Q5SWD9; -.
DR TreeFam; TF105717; -.
DR Reactome; R-MMU-6791226; Major pathway of rRNA processing in the nucleolus and cytosol.
DR BioGRID-ORCS; 104662; 24 hits in 75 CRISPR screens.
DR ChiTaRS; Tsr1; mouse.
DR PRO; PR:Q5SWD9; -.
DR Proteomes; UP000000589; Chromosome 11.
DR RNAct; Q5SWD9; protein.
DR Bgee; ENSMUSG00000038335; Expressed in primitive streak and 251 other tissues.
DR ExpressionAtlas; Q5SWD9; baseline and differential.
DR Genevisible; Q5SWD9; MM.
DR GO; GO:0005730; C:nucleolus; ISS:UniProtKB.
DR GO; GO:0030688; C:preribosome, small subunit precursor; IBA:GO_Central.
DR GO; GO:0005525; F:GTP binding; IBA:GO_Central.
DR GO; GO:0003924; F:GTPase activity; IBA:GO_Central.
DR GO; GO:0034511; F:U3 snoRNA binding; IBA:GO_Central.
DR GO; GO:0000479; P:endonucleolytic cleavage of tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA); IBA:GO_Central.
DR GO; GO:0000462; P:maturation of SSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA); IBA:GO_Central.
DR InterPro; IPR012948; AARP2CN.
DR InterPro; IPR039761; Bms1/Tsr1.
DR InterPro; IPR007034; BMS1_TSR1_C.
DR InterPro; IPR030387; G_Bms1/Tsr1_dom.
DR PANTHER; PTHR12858; PTHR12858; 1.
DR Pfam; PF08142; AARP2CN; 1.
DR Pfam; PF04950; RIBIOP_C; 1.
DR SMART; SM00785; AARP2CN; 1.
DR SMART; SM01362; DUF663; 1.
DR PROSITE; PS51714; G_BMS1; 1.
PE 1: Evidence at protein level;
KW Alternative splicing; Nucleus; Reference proteome; Ribosome biogenesis.
FT CHAIN 1..803
FT /note="Pre-rRNA-processing protein TSR1 homolog"
FT /id="PRO_0000311275"
FT DOMAIN 81..242
FT /note="Bms1-type G"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01051"
FT REGION 1..83
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 428..447
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 62..78
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 432..446
FT /note="Acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT VAR_SEQ 1..122
FT /note="Missing (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:16141072"
FT /id="VSP_029504"
FT VAR_SEQ 381..382
FT /note="DL -> GQ (in isoform 3)"
FT /evidence="ECO:0000303|PubMed:16141072"
FT /id="VSP_029505"
FT VAR_SEQ 383..803
FT /note="Missing (in isoform 3)"
FT /evidence="ECO:0000303|PubMed:16141072"
FT /id="VSP_029506"
FT CONFLICT 210
FT /note="M -> T (in Ref. 3; AAH31531)"
FT /evidence="ECO:0000305"
FT CONFLICT 335
FT /note="P -> A (in Ref. 3; AAH31531)"
FT /evidence="ECO:0000305"
FT CONFLICT 438
FT /note="E -> K (in Ref. 1; BAE37023)"
FT /evidence="ECO:0000305"
FT CONFLICT 464
FT /note="A -> E (in Ref. 3; AAH31531)"
FT /evidence="ECO:0000305"
FT CONFLICT 649
FT /note="F -> L (in Ref. 3; AAH31531)"
FT /evidence="ECO:0000305"
FT CONFLICT 707
FT /note="T -> N (in Ref. 1; BAE32349)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 803 AA; 92105 MW; 0F0D07FD08E96C0A CRC64;
MAAHRSGPLK QQNKAHKGGR HHGGGSAQRD SKGRVGPKIL CKKLKRQLSR IDQRHRASQL
RKQKRESVLA EKRQLGSKDG PPHQVLVVPL HSRISLPEAF KLLQNEDLGT VYLSERGSTQ
SFMLLCPSLK HRWFFTYARP GDLHTLLDMA KVADTILFLL DPLEGWDSTG DYCLSCLFAQ
GLPTYTLAVQ GLSGFPPKKQ IDARKKLSKM VEKRFPEDKL LLLDTQQESG MLLRQLANQK
QRHLAFRDRR AYLFAHVADF VPSEESDLVG TLKISGYVRG RTLNVNSLLH IVGHGDFQMN
QIDAPVDPFP LNPRVIKSQK KPNMAMEVCV TDAAPDMEED LKVLMKADPD HQESLQTEAI
PDPMEGEQTW PTEEELDEAD DLLKQRSRVV KKVPKGTSSY QAEWILDEGD ESDGEGGEYD
DIQHEGFMEE ESQDGSGEEE EEECETMTLG ESVRDDLYDE KVDAEDEERM LEKYKQERLE
EMFPDEMDTP RDVAARIRFQ KYRGLKSFRT SPWDPKENLP RDYARIFQFQ NFVNTRKRIF
KEIEEKEAEG AEVGWYVTLH VSDVPVSVVE YFRQGAPLIA FSLLPYEQKM SVLNMVVSRN
PGNTEPVKAK EELIFHCGFR RFRASPLFSQ HTAADKHKFQ RFLTADAAFV VTVFAPITFP
PASVLLFKQR RNGMHSLIAT GHLFSVDPDR MVIKRVVLSG HPFKIFTKMA VVRYMFFNRE
DVMWFKPVEL RTKWGRRGHI KEPLGTHGHM KCSFDGKLKS QDTVLMNLYK RVFPKWTYDP
YVPEPVPWVK SDISSTVSEV DME
