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TSSB1_PSEAE
ID   TSSB1_PSEAE             Reviewed;         172 AA.
AC   Q9I749;
DT   22-APR-2020, integrated into UniProtKB/Swiss-Prot.
DT   01-MAR-2001, sequence version 1.
DT   03-AUG-2022, entry version 98.
DE   RecName: Full=Type VI secretion system sheath protein TssB1 {ECO:0000303|PubMed:22906320};
DE   AltName: Full=Sheath protein HsiB1;
GN   Name=tssB1 {ECO:0000303|PubMed:22906320}; OrderedLocusNames=PA0083;
OS   Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM
OS   14847 / LMG 12228 / 1C / PRS 101 / PAO1).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Pseudomonadales;
OC   Pseudomonadaceae; Pseudomonas.
OX   NCBI_TaxID=208964;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C /
RC   PRS 101 / PAO1;
RX   PubMed=10984043; DOI=10.1038/35023079;
RA   Stover C.K., Pham X.-Q.T., Erwin A.L., Mizoguchi S.D., Warrener P.,
RA   Hickey M.J., Brinkman F.S.L., Hufnagle W.O., Kowalik D.J., Lagrou M.,
RA   Garber R.L., Goltry L., Tolentino E., Westbrock-Wadman S., Yuan Y.,
RA   Brody L.L., Coulter S.N., Folger K.R., Kas A., Larbig K., Lim R.M.,
RA   Smith K.A., Spencer D.H., Wong G.K.-S., Wu Z., Paulsen I.T., Reizer J.,
RA   Saier M.H. Jr., Hancock R.E.W., Lory S., Olson M.V.;
RT   "Complete genome sequence of Pseudomonas aeruginosa PAO1, an opportunistic
RT   pathogen.";
RL   Nature 406:959-964(2000).
RN   [2]
RP   FUNCTION, INTERACTION WITH TAGJ, AND DISRUPTION PHENOTYPE.
RC   STRAIN=PAK;
RX   PubMed=22906320; DOI=10.1111/j.1365-2958.2012.08204.x;
RA   Lossi N.S., Manoli E., Simpson P., Jones C., Hui K., Dajani R.,
RA   Coulthurst S.J., Freemont P., Filloux A.;
RT   "The archetype Pseudomonas aeruginosa proteins TssB and TagJ form a novel
RT   subcomplex in the bacterial type VI secretion system.";
RL   Mol. Microbiol. 86:437-456(2012).
RN   [3]
RP   FUNCTION, AND INTERACTION WITH TSSC1.
RX   PubMed=23341461; DOI=10.1074/jbc.m112.439273;
RA   Lossi N.S., Manoli E., Foerster A., Dajani R., Pape T., Freemont P.,
RA   Filloux A.;
RT   "The HsiB1C1 (TssB-TssC) complex of the Pseudomonas aeruginosa type VI
RT   secretion system forms a bacteriophage tail sheathlike structure.";
RL   J. Biol. Chem. 288:7536-7548(2013).
RN   [4]
RP   INTERACTION WITH TSSA1.
RX   PubMed=27288401; DOI=10.15252/embj.201694024;
RA   Planamente S., Salih O., Manoli E., Albesa-Jove D., Freemont P.S.,
RA   Filloux A.;
RT   "TssA forms a gp6-like ring attached to the type VI secretion sheath.";
RL   EMBO J. 35:1613-1627(2016).
RN   [5] {ECO:0007744|PDB:4UQY, ECO:0007744|PDB:4UQZ}
RP   X-RAY CRYSTALLOGRAPHY (1.60 ANGSTROMS), INTERACTION WITH TAGJ AND CLPV1,
RP   AND FUNCTION.
RX   PubMed=25305017; DOI=10.1074/jbc.m114.600510;
RA   Foerster A., Planamente S., Manoli E., Lossi N.S., Freemont P.S.,
RA   Filloux A.;
RT   "Coevolution of the ATPase ClpV, the sheath proteins TssB and TssC, and the
RT   accessory protein TagJ/HsiE1 distinguishes type VI secretion classes.";
RL   J. Biol. Chem. 289:33032-33043(2014).
RN   [6] {ECO:0007744|PDB:5N8N}
RP   STRUCTURE BY ELECTRON MICROSCOPY (3.28 ANGSTROMS) OF 4-135, FUNCTION, AND
RP   INTERACTION WITH TSSC1.
RX   PubMed=29307484; DOI=10.1016/j.str.2017.12.005;
RA   Salih O., He S., Planamente S., Stach L., MacDonald J.T., Manoli E.,
RA   Scheres S.H.W., Filloux A., Freemont P.S.;
RT   "Atomic Structure of Type VI Contractile Sheath from Pseudomonas
RT   aeruginosa.";
RL   Structure 26:329-336.E3(2018).
CC   -!- FUNCTION: Core component of the H1 type VI (H1-T6SS) secretion system
CC       that plays a role in the release of toxins targeting both eukaryotic
CC       and prokaryotic species. Forms the sheath of the structure by
CC       assembling into tubules together with TssC1 resulting in the stacking
CC       of cogwheel-like structures showing predominantly a 12-fold symmetry
CC       (PubMed:23341461, PubMed:25305017, PubMed:22906320). The sheath
CC       contracts to provide the energy needed for effector delivery
CC       (PubMed:29307484). {ECO:0000269|PubMed:22906320,
CC       ECO:0000269|PubMed:23341461, ECO:0000269|PubMed:25305017,
CC       ECO:0000269|PubMed:29307484}.
CC   -!- SUBUNIT: Forms a heterodimer with TssC1. Heterodimers assemble to form
CC       the sheath of the T6SS machinery (PubMed:29307484). Interacts with TagJ
CC       (PubMed:22906320, PubMed:25305017). Interacts with TssA1
CC       (PubMed:27288401). {ECO:0000269|PubMed:22906320,
CC       ECO:0000269|PubMed:25305017, ECO:0000269|PubMed:27288401,
CC       ECO:0000269|PubMed:29307484}.
CC   -!- DISRUPTION PHENOTYPE: Deletion mutant shows a clear reduction in E.
CC       coli killing. {ECO:0000269|PubMed:22906320}.
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DR   EMBL; AE004091; AAG03473.1; -; Genomic_DNA.
DR   PIR; H83634; H83634.
DR   RefSeq; NP_248773.1; NC_002516.2.
DR   RefSeq; WP_003083666.1; NZ_QZGE01000015.1.
DR   PDB; 4UQY; X-ray; 1.60 A; B=1-20.
DR   PDB; 4UQZ; X-ray; 1.60 A; B=1-172.
DR   PDB; 5N8N; EM; 3.28 A; A/C/E/G/I/K/M/O/Q/S/U/W/Y/a/c=4-135.
DR   PDBsum; 4UQY; -.
DR   PDBsum; 4UQZ; -.
DR   PDBsum; 5N8N; -.
DR   AlphaFoldDB; Q9I749; -.
DR   SMR; Q9I749; -.
DR   STRING; 287.DR97_3040; -.
DR   PaxDb; Q9I749; -.
DR   PRIDE; Q9I749; -.
DR   DNASU; 879476; -.
DR   EnsemblBacteria; AAG03473; AAG03473; PA0083.
DR   GeneID; 879476; -.
DR   KEGG; pae:PA0083; -.
DR   PATRIC; fig|208964.12.peg.87; -.
DR   PseudoCAP; PA0083; -.
DR   HOGENOM; CLU_111033_0_1_6; -.
DR   InParanoid; Q9I749; -.
DR   OMA; MTFESME; -.
DR   PhylomeDB; Q9I749; -.
DR   BioCyc; PAER208964:G1FZ6-85-MON; -.
DR   Proteomes; UP000002438; Chromosome.
DR   InterPro; IPR008312; T6SS_TssB1.
DR   PANTHER; PTHR35850; PTHR35850; 1.
DR   Pfam; PF05591; T6SS_VipA; 1.
DR   PIRSF; PIRSF028301; UCP028301; 1.
DR   TIGRFAMs; TIGR03358; VI_chp_5; 1.
PE   1: Evidence at protein level;
KW   3D-structure; Reference proteome.
FT   CHAIN           1..172
FT                   /note="Type VI secretion system sheath protein TssB1"
FT                   /id="PRO_0000449262"
FT   HELIX           7..14
FT                   /evidence="ECO:0007829|PDB:5N8N"
FT   STRAND          18..23
FT                   /evidence="ECO:0007829|PDB:5N8N"
FT   STRAND          26..28
FT                   /evidence="ECO:0007829|PDB:4UQZ"
FT   STRAND          38..44
FT                   /evidence="ECO:0007829|PDB:5N8N"
FT   STRAND          63..65
FT                   /evidence="ECO:0007829|PDB:5N8N"
FT   TURN            67..69
FT                   /evidence="ECO:0007829|PDB:5N8N"
FT   HELIX           70..77
FT                   /evidence="ECO:0007829|PDB:5N8N"
FT   STRAND          80..86
FT                   /evidence="ECO:0007829|PDB:5N8N"
FT   STRAND          89..91
FT                   /evidence="ECO:0007829|PDB:5N8N"
FT   STRAND          93..100
FT                   /evidence="ECO:0007829|PDB:5N8N"
FT   HELIX           104..107
FT                   /evidence="ECO:0007829|PDB:5N8N"
FT   HELIX           109..115
FT                   /evidence="ECO:0007829|PDB:5N8N"
FT   HELIX           117..133
FT                   /evidence="ECO:0007829|PDB:5N8N"
SQ   SEQUENCE   172 AA;  18786 MW;  655C9C0B5C9D97F5 CRC64;
     MGSTTSSQKF IARNRAPRVQ IEYDVELYGA EKKVQLPFVM GVMADLAGKP AEPQAAVADR
     KFLEIDVDNF DARLKAMKPR VAFNVPNVLT GEGNLSLDIT FESMDDFSPA AVARKVDSLN
     KLLEARTQLA NLLTYMDGKT GAEEMIMKAI KDPALLQALA SAPKPKDDEP QA
 
 
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