ID   TSSK1_PSEAE             Reviewed;         444 AA.
AC   Q9I753;
DT   22-APR-2020, integrated into UniProtKB/Swiss-Prot.
DT   01-MAR-2001, sequence version 1.
DT   03-AUG-2022, entry version 85.
DE   RecName: Full=Type VI secretion system baseplate component TssK1 {ECO:0000303|PubMed:27288401};
GN   Name=tssK1 {ECO:0000303|PubMed:27288401}; OrderedLocusNames=PA0079;
OS   Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM
OS   14847 / LMG 12228 / 1C / PRS 101 / PAO1).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Pseudomonadales;
OC   Pseudomonadaceae; Pseudomonas.
OX   NCBI_TaxID=208964;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C /
RC   PRS 101 / PAO1;
RX   PubMed=10984043; DOI=10.1038/35023079;
RA   Stover C.K., Pham X.-Q.T., Erwin A.L., Mizoguchi S.D., Warrener P.,
RA   Hickey M.J., Brinkman F.S.L., Hufnagle W.O., Kowalik D.J., Lagrou M.,
RA   Garber R.L., Goltry L., Tolentino E., Westbrock-Wadman S., Yuan Y.,
RA   Brody L.L., Coulter S.N., Folger K.R., Kas A., Larbig K., Lim R.M.,
RA   Smith K.A., Spencer D.H., Wong G.K.-S., Wu Z., Paulsen I.T., Reizer J.,
RA   Saier M.H. Jr., Hancock R.E.W., Lory S., Olson M.V.;
RT   "Complete genome sequence of Pseudomonas aeruginosa PAO1, an opportunistic
RT   pathogen.";
RL   Nature 406:959-964(2000).
RN   [2]
RP   INTERACTION WITH TSSA1.
RX   PubMed=27288401; DOI=10.15252/embj.201694024;
RA   Planamente S., Salih O., Manoli E., Albesa-Jove D., Freemont P.S.,
RA   Filloux A.;
RT   "TssA forms a gp6-like ring attached to the type VI secretion sheath.";
RL   EMBO J. 35:1613-1627(2016).
RN   [3]
RP   FUNCTION, DISRUPTION PHENOTYPE, AND SUBUNIT.
RX   PubMed=31379775; DOI=10.3389/fmicb.2019.01615;
RA   Liebl D., Robert-Genthon M., Job V., Cogoni V., Attree I.;
RT   "Baseplate component TssK and spatio-temporal assembly of T6SS in
RT   Pseudomonas aeruginosa.";
RL   Front. Microbiol. 10:1615-1615(2019).
CC   -!- FUNCTION: Core component of the H1 type VI (H1-T6SS) secretion system
CC       that plays a role in the release of toxins targeting both eukaryotic
CC       and prokaryotic species. Functions as a spatio-temporal marker for
CC       assembly of contractile apparatus made of TssB1 and TssC1. This role in
CC       assembly depends on TssM1. {ECO:0000269|PubMed:31379775}.
CC   -!- SUBUNIT: Forms transient higher-order structures that correlated with
CC       dynamics of sheath component TssB1 (PubMed:31379775). Interacts with
CC       TssA1 (PubMed:27288401). {ECO:0000269|PubMed:27288401,
CC       ECO:0000269|PubMed:31379775}.
CC   -!- DISRUPTION PHENOTYPE: Deletion mutant shows a severely reduced
CC       secretion of VgrG1a, VgrG1c as well as Hcp1.
CC       {ECO:0000269|PubMed:31379775}.
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DR   EMBL; AE004091; AAG03469.1; -; Genomic_DNA.
DR   PIR; D83634; D83634.
DR   RefSeq; NP_248769.1; NC_002516.2.
DR   RefSeq; WP_003115052.1; NZ_QZGE01000015.1.
DR   AlphaFoldDB; Q9I753; -.
DR   SMR; Q9I753; -.
DR   STRING; 287.DR97_3036; -.
DR   PaxDb; Q9I753; -.
DR   PRIDE; Q9I753; -.
DR   EnsemblBacteria; AAG03469; AAG03469; PA0079.
DR   GeneID; 879382; -.
DR   KEGG; pae:PA0079; -.
DR   PATRIC; fig|208964.12.peg.82; -.
DR   PseudoCAP; PA0079; -.
DR   HOGENOM; CLU_031690_3_0_6; -.
DR   InParanoid; Q9I753; -.
DR   OMA; YTYFELD; -.
DR   PhylomeDB; Q9I753; -.
DR   BioCyc; PAER208964:G1FZ6-81-MON; -.
DR   Proteomes; UP000002438; Chromosome.
DR   InterPro; IPR010263; T6SS_TssK.
DR   PANTHER; PTHR35566; PTHR35566; 1.
DR   Pfam; PF05936; T6SS_VasE; 1.
DR   TIGRFAMs; TIGR03353; VI_chp_4; 1.
PE   1: Evidence at protein level;
KW   Reference proteome.
FT   CHAIN           1..444
FT                   /note="Type VI secretion system baseplate component TssK1"
FT                   /id="PRO_0000449266"
SQ   SEQUENCE   444 AA;  48275 MW;  EE0E772FA6BAA5A5 CRC64;
     MSWNNRVVWS EGMFLRPQHF QQHDRYLETL VDGRCRSLLA GGWGFSELKL DDALLTQGKL
     AIVSARGVLP DGTPFNIPAD DPAPAPLNVE ESLRDGIVYL GLPLKRVGTR DTVEEGEALG
     GARYVSQVQE VRDDNAAFES RAPVALGSQA FRLLTERDGL GEYAAVGVAR VREKRADQAL
     SLDEDYLPPV LDIAAAPPLA SFAKELLGLL HQRGEALAGR VVASSAGGAS EIADFLLLQL
     VNRAEALTGH LSRVRPLHPQ ELYRELVALA GEFCTFTASQ RRPEEYPVYN HDDLAASFAP
     VMLALRQALA TVIDAKAIAI PIVEKAYGVH VAMLSDRSLI DNASFVLVVR ADVPGESLRG
     HFPQQAKVGS VEHIRDLVNL QLPGIGLLPM PVAPRQIPYH AGSTYFELDR GSAHWKQLTH
     SGGFAFHIAG QFPGLNLAFW AIRG