ID   TSSM1_PSEAE             Reviewed;        1101 AA.
AC   Q9I755;
DT   22-APR-2020, integrated into UniProtKB/Swiss-Prot.
DT   01-MAR-2001, sequence version 1.
DT   03-AUG-2022, entry version 97.
DE   RecName: Full=Type VI secretion system component TssM1 {ECO:0000303|PubMed:31379775};
GN   Name=tssM1 {ECO:0000303|PubMed:31379775}; OrderedLocusNames=PA0077;
OS   Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM
OS   14847 / LMG 12228 / 1C / PRS 101 / PAO1).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Pseudomonadales;
OC   Pseudomonadaceae; Pseudomonas.
OX   NCBI_TaxID=208964;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C /
RC   PRS 101 / PAO1;
RX   PubMed=10984043; DOI=10.1038/35023079;
RA   Stover C.K., Pham X.-Q.T., Erwin A.L., Mizoguchi S.D., Warrener P.,
RA   Hickey M.J., Brinkman F.S.L., Hufnagle W.O., Kowalik D.J., Lagrou M.,
RA   Garber R.L., Goltry L., Tolentino E., Westbrock-Wadman S., Yuan Y.,
RA   Brody L.L., Coulter S.N., Folger K.R., Kas A., Larbig K., Lim R.M.,
RA   Smith K.A., Spencer D.H., Wong G.K.-S., Wu Z., Paulsen I.T., Reizer J.,
RA   Saier M.H. Jr., Hancock R.E.W., Lory S., Olson M.V.;
RT   "Complete genome sequence of Pseudomonas aeruginosa PAO1, an opportunistic
RT   pathogen.";
RL   Nature 406:959-964(2000).
RN   [2]
RP   SUBCELLULAR LOCATION.
RC   STRAIN=ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C /
RC   PRS 101 / PAO1;
RX   PubMed=23744604; DOI=10.1002/pmic.201200565;
RA   Casabona M.G., Vandenbrouck Y., Attree I., Coute Y.;
RT   "Proteomic characterization of Pseudomonas aeruginosa PAO1 inner
RT   membrane.";
RL   Proteomics 13:2419-2423(2013).
RN   [3]
RP   FUNCTION, AND DISRUPTION PHENOTYPE.
RX   PubMed=31379775; DOI=10.3389/fmicb.2019.01615;
RA   Liebl D., Robert-Genthon M., Job V., Cogoni V., Attree I.;
RT   "Baseplate component TssK and spatio-temporal assembly of T6SS in
RT   Pseudomonas aeruginosa.";
RL   Front. Microbiol. 10:1615-1615(2019).
CC   -!- FUNCTION: Core component of the type VI (T6SS) secretion system that
CC       plays a role in the release of toxins targeting both eukaryotic and
CC       prokaryotic species. Plays an essential role in stabilization of
CC       assembled TssK1 structure at a fixed perimembrane site.
CC       {ECO:0000269|PubMed:31379775}.
CC   -!- SUBCELLULAR LOCATION: Cell inner membrane
CC       {ECO:0000269|PubMed:23744604}; Single-pass membrane protein
CC       {ECO:0000255}.
CC   -!- DISRUPTION PHENOTYPE: In the deletion mutant, TssB1 is still recruited
CC       to the structure containing TssK1, but the assembly of TssB1-sheath is
CC       aborted likely at the nucleation stage. {ECO:0000269|PubMed:31379775}.
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DR   EMBL; AE004091; AAG03467.1; -; Genomic_DNA.
DR   PIR; G83637; G83637.
DR   RefSeq; NP_248767.1; NC_002516.2.
DR   RefSeq; WP_010895493.1; NC_002516.2.
DR   AlphaFoldDB; Q9I755; -.
DR   SMR; Q9I755; -.
DR   STRING; 287.DR97_3034; -.
DR   PaxDb; Q9I755; -.
DR   PRIDE; Q9I755; -.
DR   EnsemblBacteria; AAG03467; AAG03467; PA0077.
DR   GeneID; 879396; -.
DR   KEGG; pae:PA0077; -.
DR   PATRIC; fig|208964.12.peg.80; -.
DR   PseudoCAP; PA0077; -.
DR   HOGENOM; CLU_003353_2_1_6; -.
DR   InParanoid; Q9I755; -.
DR   OMA; GRQPING; -.
DR   PhylomeDB; Q9I755; -.
DR   BioCyc; PAER208964:G1FZ6-79-MON; -.
DR   Proteomes; UP000002438; Chromosome.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR   InterPro; IPR009612; IcmF-related.
DR   InterPro; IPR025743; IcmF-related_N_dom.
DR   InterPro; IPR010623; IcmF_C.
DR   InterPro; IPR027417; P-loop_NTPase.
DR   InterPro; IPR017731; TssM1-like.
DR   Pfam; PF06761; IcmF-related; 1.
DR   Pfam; PF14331; IcmF-related_N; 1.
DR   Pfam; PF06744; IcmF_C; 1.
DR   SUPFAM; SSF52540; SSF52540; 1.
DR   TIGRFAMs; TIGR03348; VI_IcmF; 1.
PE   3: Inferred from homology;
KW   Cell inner membrane; Cell membrane; Membrane; Reference proteome;
KW   Transmembrane; Transmembrane helix.
FT   CHAIN           1..1101
FT                   /note="Type VI secretion system component TssM1"
FT                   /id="PRO_0000449267"
FT   TRANSMEM        371..391
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
SQ   SEQUENCE   1101 AA;  121460 MW;  216CCAD5E6F768CE CRC64;
     MQSLAEVSAP DAASVATAEE LATLKQRMDE ALALLKRAKL GGSERRNLYE LPWYVIIGPP
     GSGKTTALMN SGLDFPLAAQ MGAGAIRGVG GTRNCDWWFT DEAVLLDTAG RYTTQDSHAQ
     VDKAAWLGFL DLLKTQRKRR PIDGAFIAIS LSDLLLGSDA ERAAHAQAIR ARIQELYQQL
     GVRFPIYVML TKFDLVPGFM EFFDSLNREE RAQVWGMTFA LDDGKSAEGP LAVFDSEFAL
     LEQRLTARLV ERLQQERDPA RRDLVYGFPQ QFAALRECLG EFLNGVFKPN PYEERPLLRG
     LYFTSGTQEG SPIDRLIGSM AQSMNLDRQH LARQTGTGRS YFIERLFREV AFGERGLVGT
     NPKVERRRKW LTIGALSATA LVVLAVTAVW IASYRANQSY IAAVDQRVDP LARGIESLSP
     AQRDVLAVLP QLNAVQNLAG DAPSWAEGYG LYQGDMLGEE SASVYRKLLI AVFAPRLVTR
     IEEQLRSGGS SDFLYEGLKA YLMLGSPDHY DADFIKAWIS LDWERNLPRD LSPEQRQALH
     AHLDALLERR PPSARLDQDL VEDLRRQLQQ LPVAQRVYDR VKRQRLPKDV PDFRISDAAG
     RDAPLVFARK SGKPLTDPLS GFFTYRGYRE VFLTASLSQA GTIAEEQWVL GRDLNDAGDA
     ANLALDVRRL YFQDYLRQWD DLLADLTVVP ITNVTQAADV LRILSGPTSP FRKLLEAVAR
     ETDLQKGDRL VAAQVKKAAD GTVDKLKQRL GSLVGQEEEG AREQPRQVDS DPISAHFAEL
     NSLVSKGEGG NEPAPIDSLL EDMNALYVQV SAMAGASGDS LLGDAKNQVA AAASRVALSA
     ERQPPVVQGL VKNVVNSTTS SMMGSVRNQL NAAWISDVVS VYRQSLAGRY PIAAGSSRDA
     TLEDFGHFFG AGGVMDSYFR QYLQPYVDTS ASTWRWQPGA AQKLGINPGV LHTFQRAAAI
     RDAFFRSGGM QPTVRFELKP VTMDAAISQF ILDLDGQQLT YDHGPSRPVA MQWPSANGLG
     VVRLTVTPPP SSGRSGLTLE GPWAWFRLLD QSDLERGNSP DRFTLRLRID GSSIACELRA
     SSAFNPFKSR VVSGFSLPER L