ID   TSSP_HUMAN              Reviewed;         514 AA.
AC   Q9NQE7; O75416;
DT   27-APR-2001, integrated into UniProtKB/Swiss-Prot.
DT   27-APR-2001, sequence version 2.
DT   03-AUG-2022, entry version 145.
DE   RecName: Full=Thymus-specific serine protease;
DE            EC=3.4.-.-;
DE   AltName: Full=Serine protease 16;
DE   Flags: Precursor;
GN   Name=PRSS16; Synonyms=TSSP;
OS   Homo sapiens (Human).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC   Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae;
OC   Homo.
OX   NCBI_TaxID=9606;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA].
RC   TISSUE=Thymus;
RX   PubMed=10527559; DOI=10.1006/cimm.1999.1543;
RA   Bowlus C.L., Ahn J., Chu T., Gruen J.R.;
RT   "Cloning of a novel MHC-encoded serine peptidase highly expressed by
RT   cortical epithelial cells of the thymus.";
RL   Cell. Immunol. 196:80-86(1999).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX   PubMed=14574404; DOI=10.1038/nature02055;
RA   Mungall A.J., Palmer S.A., Sims S.K., Edwards C.A., Ashurst J.L.,
RA   Wilming L., Jones M.C., Horton R., Hunt S.E., Scott C.E., Gilbert J.G.R.,
RA   Clamp M.E., Bethel G., Milne S., Ainscough R., Almeida J.P., Ambrose K.D.,
RA   Andrews T.D., Ashwell R.I.S., Babbage A.K., Bagguley C.L., Bailey J.,
RA   Banerjee R., Barker D.J., Barlow K.F., Bates K., Beare D.M., Beasley H.,
RA   Beasley O., Bird C.P., Blakey S.E., Bray-Allen S., Brook J., Brown A.J.,
RA   Brown J.Y., Burford D.C., Burrill W., Burton J., Carder C., Carter N.P.,
RA   Chapman J.C., Clark S.Y., Clark G., Clee C.M., Clegg S., Cobley V.,
RA   Collier R.E., Collins J.E., Colman L.K., Corby N.R., Coville G.J.,
RA   Culley K.M., Dhami P., Davies J., Dunn M., Earthrowl M.E., Ellington A.E.,
RA   Evans K.A., Faulkner L., Francis M.D., Frankish A., Frankland J.,
RA   French L., Garner P., Garnett J., Ghori M.J., Gilby L.M., Gillson C.J.,
RA   Glithero R.J., Grafham D.V., Grant M., Gribble S., Griffiths C.,
RA   Griffiths M.N.D., Hall R., Halls K.S., Hammond S., Harley J.L., Hart E.A.,
RA   Heath P.D., Heathcott R., Holmes S.J., Howden P.J., Howe K.L., Howell G.R.,
RA   Huckle E., Humphray S.J., Humphries M.D., Hunt A.R., Johnson C.M.,
RA   Joy A.A., Kay M., Keenan S.J., Kimberley A.M., King A., Laird G.K.,
RA   Langford C., Lawlor S., Leongamornlert D.A., Leversha M., Lloyd C.R.,
RA   Lloyd D.M., Loveland J.E., Lovell J., Martin S., Mashreghi-Mohammadi M.,
RA   Maslen G.L., Matthews L., McCann O.T., McLaren S.J., McLay K., McMurray A.,
RA   Moore M.J.F., Mullikin J.C., Niblett D., Nickerson T., Novik K.L.,
RA   Oliver K., Overton-Larty E.K., Parker A., Patel R., Pearce A.V., Peck A.I.,
RA   Phillimore B.J.C.T., Phillips S., Plumb R.W., Porter K.M., Ramsey Y.,
RA   Ranby S.A., Rice C.M., Ross M.T., Searle S.M., Sehra H.K., Sheridan E.,
RA   Skuce C.D., Smith S., Smith M., Spraggon L., Squares S.L., Steward C.A.,
RA   Sycamore N., Tamlyn-Hall G., Tester J., Theaker A.J., Thomas D.W.,
RA   Thorpe A., Tracey A., Tromans A., Tubby B., Wall M., Wallis J.M.,
RA   West A.P., White S.S., Whitehead S.L., Whittaker H., Wild A., Willey D.J.,
RA   Wilmer T.E., Wood J.M., Wray P.W., Wyatt J.C., Young L., Younger R.M.,
RA   Bentley D.R., Coulson A., Durbin R.M., Hubbard T., Sulston J.E., Dunham I.,
RA   Rogers J., Beck S.;
RT   "The DNA sequence and analysis of human chromosome 6.";
RL   Nature 425:805-811(2003).
CC   -!- FUNCTION: Protease that may play a role in T-cell development.
CC   -!- SUBCELLULAR LOCATION: Cytoplasmic vesicle. Note=Vesicular, either
CC       lysosomal or endosomal.
CC   -!- TISSUE SPECIFICITY: Expressed predominantly in cortical thymic
CC       epithelial cells.
CC   -!- DEVELOPMENTAL STAGE: Expressed in fetal thymus.
CC   -!- SIMILARITY: Belongs to the peptidase S28 family. {ECO:0000305}.
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DR   EMBL; AF052514; AAC33563.1; -; mRNA.
DR   EMBL; AL021808; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR   CCDS; CCDS4623.1; -.
DR   RefSeq; NP_005856.1; NM_005865.3.
DR   AlphaFoldDB; Q9NQE7; -.
DR   SMR; Q9NQE7; -.
DR   BioGRID; 115568; 133.
DR   IntAct; Q9NQE7; 1.
DR   MINT; Q9NQE7; -.
DR   STRING; 9606.ENSP00000230582; -.
DR   ESTHER; human-PRSS16; Prolylcarboxypeptidase.
DR   MEROPS; S28.003; -.
DR   GlyGen; Q9NQE7; 3 sites.
DR   iPTMnet; Q9NQE7; -.
DR   PhosphoSitePlus; Q9NQE7; -.
DR   BioMuta; PRSS16; -.
DR   DMDM; 13633990; -.
DR   MassIVE; Q9NQE7; -.
DR   PaxDb; Q9NQE7; -.
DR   PeptideAtlas; Q9NQE7; -.
DR   PRIDE; Q9NQE7; -.
DR   Antibodypedia; 1724; 110 antibodies from 21 providers.
DR   DNASU; 10279; -.
DR   Ensembl; ENST00000230582.8; ENSP00000230582.3; ENSG00000112812.16.
DR   GeneID; 10279; -.
DR   KEGG; hsa:10279; -.
DR   MANE-Select; ENST00000230582.8; ENSP00000230582.3; NM_005865.4; NP_005856.1.
DR   UCSC; uc003nja.4; human.
DR   CTD; 10279; -.
DR   DisGeNET; 10279; -.
DR   GeneCards; PRSS16; -.
DR   HGNC; HGNC:9480; PRSS16.
DR   HPA; ENSG00000112812; Tissue enriched (lymphoid).
DR   MIM; 607169; gene.
DR   neXtProt; NX_Q9NQE7; -.
DR   OpenTargets; ENSG00000112812; -.
DR   PharmGKB; PA33832; -.
DR   VEuPathDB; HostDB:ENSG00000112812; -.
DR   eggNOG; KOG2182; Eukaryota.
DR   GeneTree; ENSGT00940000160281; -.
DR   HOGENOM; CLU_020959_3_1_1; -.
DR   InParanoid; Q9NQE7; -.
DR   OMA; VGGSEKC; -.
DR   OrthoDB; 555765at2759; -.
DR   PhylomeDB; Q9NQE7; -.
DR   TreeFam; TF314855; -.
DR   PathwayCommons; Q9NQE7; -.
DR   SignaLink; Q9NQE7; -.
DR   BioGRID-ORCS; 10279; 10 hits in 1074 CRISPR screens.
DR   ChiTaRS; PRSS16; human.
DR   GeneWiki; PRSS16; -.
DR   GenomeRNAi; 10279; -.
DR   Pharos; Q9NQE7; Tbio.
DR   PRO; PR:Q9NQE7; -.
DR   Proteomes; UP000005640; Chromosome 6.
DR   RNAct; Q9NQE7; protein.
DR   Bgee; ENSG00000112812; Expressed in thymus and 112 other tissues.
DR   ExpressionAtlas; Q9NQE7; baseline and differential.
DR   Genevisible; Q9NQE7; HS.
DR   GO; GO:0005768; C:endosome; IDA:UniProtKB.
DR   GO; GO:0005764; C:lysosome; IDA:UniProtKB.
DR   GO; GO:0008239; F:dipeptidyl-peptidase activity; IBA:GO_Central.
DR   GO; GO:0008236; F:serine-type peptidase activity; NAS:UniProtKB.
DR   GO; GO:0030163; P:protein catabolic process; NAS:UniProtKB.
DR   GO; GO:0006508; P:proteolysis; IEA:UniProtKB-KW.
DR   Gene3D; 1.20.120.980; -; 1.
DR   Gene3D; 3.40.50.1820; -; 1.
DR   InterPro; IPR029058; AB_hydrolase.
DR   InterPro; IPR008758; Peptidase_S28.
DR   InterPro; IPR042269; Ser_carbopepase_S28_SKS.
DR   Pfam; PF05577; Peptidase_S28; 1.
DR   SUPFAM; SSF53474; SSF53474; 1.
PE   2: Evidence at transcript level;
KW   Cytoplasmic vesicle; Glycoprotein; Hydrolase; Protease; Reference proteome;
KW   Serine protease; Signal.
FT   SIGNAL          1..24
FT                   /evidence="ECO:0000255"
FT   CHAIN           25..514
FT                   /note="Thymus-specific serine protease"
FT                   /id="PRO_0000027320"
FT   ACT_SITE        185
FT                   /note="Charge relay system"
FT                   /evidence="ECO:0000255"
FT   ACT_SITE        447
FT                   /note="Charge relay system"
FT                   /evidence="ECO:0000255"
FT   ACT_SITE        472
FT                   /note="Charge relay system"
FT                   /evidence="ECO:0000255"
FT   CARBOHYD        70
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255"
FT   CARBOHYD        172
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255"
FT   CARBOHYD        321
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255"
FT   VARIANT         104
FT                   /note="S -> I (in dbSNP:rs5030965)"
FT                   /id="VAR_051826"
FT   VARIANT         204
FT                   /note="A -> G (in dbSNP:rs35466700)"
FT                   /id="VAR_051827"
SQ   SEQUENCE   514 AA;  55049 MW;  EF39BC06C560709A CRC64;
     MAVWLAQWLG PLLLVSLWGL LAPASLLRRL GEHIQQFQES SAQGLGLSLG PGAAALPKVG
     WLEQLLDPFN VSDRRSFLQR YWVNDQHWVG QDGPIFLHLG GEGSLGPGSV MRGHPAALAP
     AWGALVISLE HRFYGLSIPA GGLEMAQLRF LSSRLALADV VSARLALSRL FNISSSSPWI
     CFGGSYAGSL AAWARLKFPH LIFASVASSA PVRAVLDFSE YNDVVSRSLM STAIGGSLEC
     RAAVSVAFAE VERRLRSGGA AQAALRTELS ACGPLGRAEN QAELLGALQA LVGGVVQYDG
     QTGAPLSVRQ LCGLLLGGGG NRSHSTPYCG LRRAVQIVLH SLGQKCLSFS RAETVAQLRS
     TEPQLSGVGD RQWLYQTCTE FGFYVTCENP RCPFSQLPAL PSQLDLCEQV FGLSALSVAQ
     AVAQTNSYYG GQTPGANKVL FVNGDTDPWH VLSVTQALGS SESTLLIRTG SHCLDMAPER
     PSDSPSLRLG RQNIFQQLQT WLKLAKESQI KGEV