TSSP_MOUSE
ID TSSP_MOUSE Reviewed; 509 AA.
AC Q9QXE5;
DT 27-APR-2001, integrated into UniProtKB/Swiss-Prot.
DT 01-MAY-2000, sequence version 1.
DT 03-AUG-2022, entry version 152.
DE RecName: Full=Thymus-specific serine protease;
DE EC=3.4.-.-;
DE AltName: Full=Serine protease 16;
DE Flags: Precursor;
GN Name=Prss16; Synonyms=Tssp;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA].
RC STRAIN=C57BL/6J; TISSUE=Embryonic thymus;
RX PubMed=10630289; DOI=10.1007/s002510050601;
RA Carrier A., Nguyen C., Victorero G., Granjeaud S., Rocha D., Bernard K.,
RA Miazek A., Ferrier P., Malissen M., Naquet P., Malissen B., Jordan B.R.;
RT "Differential gene expression in CD3epsilon- and RAG1-deficient thymuses:
RT definition of a set of genes potentially involved in thymocyte
RT maturation.";
RL Immunogenetics 50:255-270(1999).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=NOD; TISSUE=Thymus;
RX PubMed=16141072; DOI=10.1126/science.1112014;
RA Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N.,
RA Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K.,
RA Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.,
RA Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R.,
RA Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T.,
RA Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A.,
RA Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B.,
RA Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M.,
RA Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S.,
RA Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E.,
RA Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D.,
RA Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M.,
RA Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H.,
RA Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V.,
RA Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S.,
RA Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H.,
RA Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N.,
RA Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F.,
RA Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G.,
RA Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z.,
RA Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C.,
RA Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y.,
RA Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S.,
RA Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K.,
RA Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R.,
RA van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H.,
RA Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M.,
RA Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C.,
RA Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S.,
RA Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K.,
RA Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M.,
RA Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C.,
RA Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A.,
RA Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.;
RT "The transcriptional landscape of the mammalian genome.";
RL Science 309:1559-1563(2005).
CC -!- FUNCTION: Protease that may play a role in T-cell development.
CC -!- SUBCELLULAR LOCATION: Cytoplasmic vesicle. Note=Vesicular, either
CC lysosomal or endosomal.
CC -!- TISSUE SPECIFICITY: Expressed predominantly in cortical thymic
CC epithelial cells, with highest expression around vessels and the thymic
CC capsule.
CC -!- DEVELOPMENTAL STAGE: Expressed in developing thymus at 14 to 18 dpc,
CC with maximal expression at 16 dpc.
CC -!- SIMILARITY: Belongs to the peptidase S28 family. {ECO:0000305}.
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DR EMBL; AJ131775; CAB66137.1; -; mRNA.
DR EMBL; AK088019; BAC40100.1; -; mRNA.
DR CCDS; CCDS26303.1; -.
DR RefSeq; NP_062302.1; NM_019429.2.
DR AlphaFoldDB; Q9QXE5; -.
DR SMR; Q9QXE5; -.
DR STRING; 10090.ENSMUSP00000006341; -.
DR ESTHER; mouse-tssp; Prolylcarboxypeptidase.
DR MEROPS; S28.003; -.
DR GlyGen; Q9QXE5; 3 sites.
DR iPTMnet; Q9QXE5; -.
DR PhosphoSitePlus; Q9QXE5; -.
DR MaxQB; Q9QXE5; -.
DR PaxDb; Q9QXE5; -.
DR PRIDE; Q9QXE5; -.
DR ProteomicsDB; 298321; -.
DR Antibodypedia; 1724; 110 antibodies from 21 providers.
DR DNASU; 54373; -.
DR Ensembl; ENSMUST00000006341; ENSMUSP00000006341; ENSMUSG00000006179.
DR GeneID; 54373; -.
DR KEGG; mmu:54373; -.
DR UCSC; uc007prz.1; mouse.
DR CTD; 10279; -.
DR MGI; MGI:1859181; Prss16.
DR VEuPathDB; HostDB:ENSMUSG00000006179; -.
DR eggNOG; KOG2182; Eukaryota.
DR GeneTree; ENSGT00940000160281; -.
DR HOGENOM; CLU_020959_3_1_1; -.
DR InParanoid; Q9QXE5; -.
DR OMA; VGGSEKC; -.
DR OrthoDB; 555765at2759; -.
DR PhylomeDB; Q9QXE5; -.
DR TreeFam; TF314855; -.
DR BioGRID-ORCS; 54373; 2 hits in 73 CRISPR screens.
DR PRO; PR:Q9QXE5; -.
DR Proteomes; UP000000589; Chromosome 13.
DR RNAct; Q9QXE5; protein.
DR Bgee; ENSMUSG00000006179; Expressed in thymus and 57 other tissues.
DR ExpressionAtlas; Q9QXE5; baseline and differential.
DR Genevisible; Q9QXE5; MM.
DR GO; GO:0005768; C:endosome; IDA:UniProtKB.
DR GO; GO:0005764; C:lysosome; IDA:UniProtKB.
DR GO; GO:0008239; F:dipeptidyl-peptidase activity; IBA:GO_Central.
DR GO; GO:0008236; F:serine-type peptidase activity; IEA:UniProtKB-KW.
DR GO; GO:0006508; P:proteolysis; IEA:UniProtKB-KW.
DR Gene3D; 1.20.120.980; -; 1.
DR Gene3D; 3.40.50.1820; -; 1.
DR InterPro; IPR029058; AB_hydrolase.
DR InterPro; IPR008758; Peptidase_S28.
DR InterPro; IPR042269; Ser_carbopepase_S28_SKS.
DR Pfam; PF05577; Peptidase_S28; 1.
DR SUPFAM; SSF53474; SSF53474; 1.
PE 2: Evidence at transcript level;
KW Cytoplasmic vesicle; Glycoprotein; Hydrolase; Protease; Reference proteome;
KW Serine protease; Signal.
FT SIGNAL 1..22
FT /evidence="ECO:0000255"
FT CHAIN 23..509
FT /note="Thymus-specific serine protease"
FT /id="PRO_0000027321"
FT ACT_SITE 184
FT /note="Charge relay system"
FT /evidence="ECO:0000255"
FT ACT_SITE 446
FT /note="Charge relay system"
FT /evidence="ECO:0000255"
FT ACT_SITE 471
FT /note="Charge relay system"
FT /evidence="ECO:0000255"
FT CARBOHYD 69
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 171
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 320
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
SQ SEQUENCE 509 AA; 54523 MW; CD8425F3F71EE43C CRC64;
MAVKAPWLGF LLLVSLWGLS TPALLLRRLR EHIQKFQESS SLHPGFGLGH GPGAVPKQGW
LEQPLDPFNA SDRRTFLQRY WVNDQHRTGQ DVPVFLHIGG EGSLGPGSVM AGHPAALAPA
WGALVISLEH RFYGLSMPAG GLDLALLRYL SSRHALADVA SARQALSGLL NVSSSSPWIC
FGGSYAGSLA TWARLKFPHL VFAAVASSAP LSAVVDFSAY NQVVARSLTQ VAIGGSLECL
AAASTAFTEV ERLLRAGPAA QAVLREELGA CGSLDLTEDQ AELLGALQAL VGGTVQYDGQ
AGAPLSVRQL CGLLLGKWGN RSRSTPYLGL RRAVQIVLRS MGQKCLSFSR AETVAQLSNT
EPQVSGVGDR QWLYQTCTEF GFYVTCEGLQ CPFSQLPALP FQLELCEQVF GLSPASVAQA
VAQTNSYYGG QSPGATQVLF VNGDTDPWHV LSVTQDLGLS EPALLIPSAS HCFDMAPMRP
SDSPSLRLGR QKISQQLQDW LKDIKKSQS
