TSST_STAAU
ID TSST_STAAU Reviewed; 234 AA.
AC P06886;
DT 01-JAN-1988, integrated into UniProtKB/Swiss-Prot.
DT 01-JAN-1988, sequence version 1.
DT 25-MAY-2022, entry version 126.
DE RecName: Full=Toxic shock syndrome toxin-1;
DE Short=TSST-1;
DE Flags: Precursor;
GN Name=tst;
OS Staphylococcus aureus.
OC Bacteria; Firmicutes; Bacilli; Bacillales; Staphylococcaceae;
OC Staphylococcus.
OX NCBI_TaxID=1280;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND PROTEIN SEQUENCE OF 41-106 AND
RP 199-224.
RC STRAIN=3-14;
RX PubMed=3782090; DOI=10.1016/s0021-9258(18)66787-0;
RA Blomster-Hautamaa D.A., Kreiswirth B.N., Kornblum J.S., Novick R.P.,
RA Schlievert P.M.;
RT "The nucleotide and partial amino acid sequence of toxic shock syndrome
RT toxin-1.";
RL J. Biol. Chem. 261:15783-15786(1986).
RN [2]
RP GENE CLONING, AND SUBCELLULAR LOCATION.
RC STRAIN=3-14;
RX PubMed=6226876; DOI=10.1038/305709a0;
RA Kreiswirth B.N., Loefdahl S., Betley M.J., O'Reilly M., Schlievert P.M.,
RA Bergdoll M.S., Novick R.P.;
RT "The toxic shock syndrome exotoxin structural gene is not detectably
RT transmitted by a prophage.";
RL Nature 305:709-712(1983).
RN [3]
RP X-RAY CRYSTALLOGRAPHY (2.5 ANGSTROMS).
RX PubMed=8107781; DOI=10.1038/367094a0;
RA Acharya K.R., Passalacqua E.F., Jones E.Y., Harlos K., Stuart D.I.,
RA Brehm R.D., Tranter H.S.;
RT "Structural basis of superantigen action inferred from crystal structure of
RT toxic-shock syndrome toxin-1.";
RL Nature 367:94-97(1994).
RN [4]
RP X-RAY CRYSTALLOGRAPHY (2.5 ANGSTROMS).
RX PubMed=8268150; DOI=10.1021/bi00213a001;
RA Prasad G.S., Earhart C.A., Murray D.L., Novick R.P., Schlivert P.M.,
RA Ohlendorf D.H.;
RT "Structure of toxic shock syndrome toxin 1.";
RL Biochemistry 32:13761-13766(1993).
RN [5]
RP X-RAY CRYSTALLOGRAPHY (2.07 ANGSTROMS).
RX PubMed=8759320; DOI=10.1006/jmbi.1996.0421;
RA Papgeorgiou A.C., Brehm R.D., Leonidas D.D., Tranter H.S., Acharya K.R.;
RT "The refined crystal structure of toxic shock syndrome toxin-1 at 2.07-A
RT resolution.";
RL J. Mol. Biol. 260:553-569(1996).
RN [6]
RP X-RAY CRYSTALLOGRAPHY (2.05 ANGSTROMS).
RX PubMed=9194182; DOI=10.1002/pro.5560060610;
RA Prasad G.S., Radhakrishnan R., Mitchell D.T., Earhart C.A., Dinges M.M.,
RA Cook W.J., Schlivert P.M., Ohlendorf D.H.;
RT "Refined structures of three crystal forms of toxic shock syndrome toxin-1
RT and of a tetramutant with reduced activity.";
RL Protein Sci. 6:1220-1227(1997).
RN [7]
RP X-RAY CRYSTALLOGRAPHY (1.95 ANGSTROMS) OF MUTANTS.
RX PubMed=9585531; DOI=10.1021/bi9721896;
RA Earhart C.A., Mitchell D.T., Murray D.L., Pinheiro D.M., Matsumura M.,
RA Schlievert P.M., Ohlendorf D.H.;
RT "Structures of five mutants of toxic shock syndrome toxin-1 with reduced
RT biological activity.";
RL Biochemistry 37:7194-7202(1998).
CC -!- FUNCTION: Responsible for the symptoms of toxic shock syndrome.
CC -!- INTERACTION:
CC PRO_0000035619; P42081-3: CD86; Xeno; NbExp=2; IntAct=EBI-16211350, EBI-15945259;
CC -!- SUBCELLULAR LOCATION: Secreted {ECO:0000269|PubMed:6226876}.
CC -!- SIMILARITY: Belongs to the staphylococcal/streptococcal toxin family.
CC {ECO:0000305}.
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DR EMBL; J02615; AAA26682.1; -; Genomic_DNA.
DR PIR; A24606; XCSAS1.
DR PDB; 1AW7; X-ray; 1.95 A; A/B/C/D=41-234.
DR PDB; 1QIL; X-ray; 2.50 A; A/B/C=41-234.
DR PDB; 1TS2; X-ray; 2.30 A; A/B/C=41-234.
DR PDB; 1TS3; X-ray; 2.00 A; A/B/C=41-234.
DR PDB; 1TS4; X-ray; 3.40 A; A/B=41-234.
DR PDB; 1TS5; X-ray; 3.10 A; A/B=41-234.
DR PDB; 2IJ0; X-ray; 2.25 A; A/B=41-234.
DR PDB; 2QIL; X-ray; 2.07 A; A/B/C=41-234.
DR PDB; 2TSS; X-ray; 2.05 A; A/B/C=41-234.
DR PDB; 3TSS; X-ray; 1.90 A; A=41-234.
DR PDB; 4OHJ; X-ray; 1.28 A; A/B=22-234.
DR PDB; 4TSS; X-ray; 2.75 A; A=41-234.
DR PDB; 5TSS; X-ray; 2.90 A; A/B=41-234.
DR PDBsum; 1AW7; -.
DR PDBsum; 1QIL; -.
DR PDBsum; 1TS2; -.
DR PDBsum; 1TS3; -.
DR PDBsum; 1TS4; -.
DR PDBsum; 1TS5; -.
DR PDBsum; 2IJ0; -.
DR PDBsum; 2QIL; -.
DR PDBsum; 2TSS; -.
DR PDBsum; 3TSS; -.
DR PDBsum; 4OHJ; -.
DR PDBsum; 4TSS; -.
DR PDBsum; 5TSS; -.
DR AlphaFoldDB; P06886; -.
DR SMR; P06886; -.
DR IntAct; P06886; 3.
DR MINT; P06886; -.
DR Allergome; 2143; Sta a TSST.
DR EvolutionaryTrace; P06886; -.
DR PRO; PR:P06886; -.
DR GO; GO:0005576; C:extracellular region; IEA:UniProtKB-SubCell.
DR GO; GO:0090729; F:toxin activity; IEA:UniProtKB-KW.
DR InterPro; IPR008992; Enterotoxin.
DR InterPro; IPR006126; Staph/Strept_toxin_CS.
DR InterPro; IPR016091; SuperAg_toxin_C.
DR InterPro; IPR013307; Superantigen_bac.
DR PRINTS; PR01501; TOXICSSTOXIN.
DR SUPFAM; SSF50203; SSF50203; 1.
DR SUPFAM; SSF54334; SSF54334; 1.
DR PROSITE; PS00278; STAPH_STREP_TOXIN_2; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Direct protein sequencing; Secreted; Signal; Superantigen;
KW Toxin; Virulence.
FT SIGNAL 1..40
FT /evidence="ECO:0000269|PubMed:3782090"
FT CHAIN 41..234
FT /note="Toxic shock syndrome toxin-1"
FT /id="PRO_0000035619"
FT HELIX 44..53
FT /evidence="ECO:0007829|PDB:4OHJ"
FT STRAND 58..68
FT /evidence="ECO:0007829|PDB:4OHJ"
FT STRAND 70..76
FT /evidence="ECO:0007829|PDB:4OHJ"
FT STRAND 82..86
FT /evidence="ECO:0007829|PDB:4OHJ"
FT STRAND 90..92
FT /evidence="ECO:0007829|PDB:1QIL"
FT STRAND 101..114
FT /evidence="ECO:0007829|PDB:4OHJ"
FT STRAND 116..118
FT /evidence="ECO:0007829|PDB:1TS3"
FT STRAND 120..126
FT /evidence="ECO:0007829|PDB:4OHJ"
FT STRAND 128..130
FT /evidence="ECO:0007829|PDB:1QIL"
FT STRAND 133..139
FT /evidence="ECO:0007829|PDB:4OHJ"
FT STRAND 143..146
FT /evidence="ECO:0007829|PDB:4OHJ"
FT STRAND 149..151
FT /evidence="ECO:0007829|PDB:3TSS"
FT STRAND 158..164
FT /evidence="ECO:0007829|PDB:4OHJ"
FT HELIX 166..181
FT /evidence="ECO:0007829|PDB:4OHJ"
FT TURN 183..185
FT /evidence="ECO:0007829|PDB:3TSS"
FT HELIX 187..189
FT /evidence="ECO:0007829|PDB:4TSS"
FT STRAND 190..198
FT /evidence="ECO:0007829|PDB:4OHJ"
FT STRAND 203..207
FT /evidence="ECO:0007829|PDB:4OHJ"
FT HELIX 214..216
FT /evidence="ECO:0007829|PDB:4OHJ"
FT HELIX 223..225
FT /evidence="ECO:0007829|PDB:4OHJ"
FT STRAND 226..234
FT /evidence="ECO:0007829|PDB:4OHJ"
SQ SEQUENCE 234 AA; 26306 MW; E95789FF9A1D7AB4 CRC64;
MNKKLLMNFF IVSPLLLATT ATDFTPVPLS SNQIIKTAKA STNDNIKDLL DWYSSGSDTF
TNSEVLDNSL GSMRIKNTDG SISLIIFPSP YYSPAFTKGE KVDLNTKRTK KSQHTSEGTY
IHFQISGVTN TEKLPTPIEL PLKVKVHGKD SPLKYGPKFD KKQLAISTLD FEIRHQLTQI
HGLYRSSDKT GGYWKITMND GSTYQSDLSK KFEYNTEKPP INIDEIKTIE AEIN
