TSTD2_HUMAN
ID TSTD2_HUMAN Reviewed; 516 AA.
AC Q5T7W7; A6NMJ4; A8K584; Q6ZQZ6; Q8IYM3; Q8WY73; Q96ML6; Q96MU1;
DT 04-APR-2006, integrated into UniProtKB/Swiss-Prot.
DT 21-DEC-2004, sequence version 1.
DT 03-AUG-2022, entry version 142.
DE RecName: Full=Thiosulfate sulfurtransferase/rhodanese-like domain-containing protein 2;
DE AltName: Full=Rhodanese domain-containing protein 2;
GN Name=TSTD2; Synonyms=C9orf97; ORFNames=PP4189;
OS Homo sapiens (Human).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae;
OC Homo.
OX NCBI_TaxID=9606;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
RX PubMed=15498874; DOI=10.1073/pnas.0404089101;
RA Wan D., Gong Y., Qin W., Zhang P., Li J., Wei L., Zhou X., Li H., Qiu X.,
RA Zhong F., He L., Yu J., Yao G., Jiang H., Qian L., Yu Y., Shu H., Chen X.,
RA Xu H., Guo M., Pan Z., Chen Y., Ge C., Yang S., Gu J.;
RT "Large-scale cDNA transfection screening for genes related to cancer
RT development and progression.";
RL Proc. Natl. Acad. Sci. U.S.A. 101:15724-15729(2004).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1), AND VARIANT GLN-83.
RC TISSUE=Trachea;
RX PubMed=14702039; DOI=10.1038/ng1285;
RA Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R.,
RA Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H.,
RA Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.,
RA Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K.,
RA Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H.,
RA Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M.,
RA Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K.,
RA Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T.,
RA Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M.,
RA Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S.,
RA Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H.,
RA Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K.,
RA Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N.,
RA Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S.,
RA Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O.,
RA Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H.,
RA Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B.,
RA Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y.,
RA Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K.,
RA Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T.,
RA Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T.,
RA Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y.,
RA Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H.,
RA Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y.,
RA Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H.,
RA Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O.,
RA Isogai T., Sugano S.;
RT "Complete sequencing and characterization of 21,243 full-length human
RT cDNAs.";
RL Nat. Genet. 36:40-45(2004).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX PubMed=15164053; DOI=10.1038/nature02465;
RA Humphray S.J., Oliver K., Hunt A.R., Plumb R.W., Loveland J.E., Howe K.L.,
RA Andrews T.D., Searle S., Hunt S.E., Scott C.E., Jones M.C., Ainscough R.,
RA Almeida J.P., Ambrose K.D., Ashwell R.I.S., Babbage A.K., Babbage S.,
RA Bagguley C.L., Bailey J., Banerjee R., Barker D.J., Barlow K.F., Bates K.,
RA Beasley H., Beasley O., Bird C.P., Bray-Allen S., Brown A.J., Brown J.Y.,
RA Burford D., Burrill W., Burton J., Carder C., Carter N.P., Chapman J.C.,
RA Chen Y., Clarke G., Clark S.Y., Clee C.M., Clegg S., Collier R.E.,
RA Corby N., Crosier M., Cummings A.T., Davies J., Dhami P., Dunn M.,
RA Dutta I., Dyer L.W., Earthrowl M.E., Faulkner L., Fleming C.J.,
RA Frankish A., Frankland J.A., French L., Fricker D.G., Garner P.,
RA Garnett J., Ghori J., Gilbert J.G.R., Glison C., Grafham D.V., Gribble S.,
RA Griffiths C., Griffiths-Jones S., Grocock R., Guy J., Hall R.E.,
RA Hammond S., Harley J.L., Harrison E.S.I., Hart E.A., Heath P.D.,
RA Henderson C.D., Hopkins B.L., Howard P.J., Howden P.J., Huckle E.,
RA Johnson C., Johnson D., Joy A.A., Kay M., Keenan S., Kershaw J.K.,
RA Kimberley A.M., King A., Knights A., Laird G.K., Langford C., Lawlor S.,
RA Leongamornlert D.A., Leversha M., Lloyd C., Lloyd D.M., Lovell J.,
RA Martin S., Mashreghi-Mohammadi M., Matthews L., McLaren S., McLay K.E.,
RA McMurray A., Milne S., Nickerson T., Nisbett J., Nordsiek G., Pearce A.V.,
RA Peck A.I., Porter K.M., Pandian R., Pelan S., Phillimore B., Povey S.,
RA Ramsey Y., Rand V., Scharfe M., Sehra H.K., Shownkeen R., Sims S.K.,
RA Skuce C.D., Smith M., Steward C.A., Swarbreck D., Sycamore N., Tester J.,
RA Thorpe A., Tracey A., Tromans A., Thomas D.W., Wall M., Wallis J.M.,
RA West A.P., Whitehead S.L., Willey D.L., Williams S.A., Wilming L.,
RA Wray P.W., Young L., Ashurst J.L., Coulson A., Blocker H., Durbin R.M.,
RA Sulston J.E., Hubbard T., Jackson M.J., Bentley D.R., Beck S., Rogers J.,
RA Dunham I.;
RT "DNA sequence and analysis of human chromosome 9.";
RL Nature 429:369-374(2004).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA], AND VARIANT GLN-83.
RA Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M.,
RA Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J.,
RA Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S.,
RA Turner R., Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H.,
RA Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K.,
RA Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D.,
RA Hunkapiller M.W., Myers E.W., Venter J.C.;
RL Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases.
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1), AND VARIANT GLN-83.
RC TISSUE=Lymph;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [6]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Cervix carcinoma;
RX PubMed=18669648; DOI=10.1073/pnas.0805139105;
RA Dephoure N., Zhou C., Villen J., Beausoleil S.A., Bakalarski C.E.,
RA Elledge S.J., Gygi S.P.;
RT "A quantitative atlas of mitotic phosphorylation.";
RL Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008).
RN [7]
RP PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-269, AND IDENTIFICATION BY
RP MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Erythroleukemia;
RX PubMed=23186163; DOI=10.1021/pr300630k;
RA Zhou H., Di Palma S., Preisinger C., Peng M., Polat A.N., Heck A.J.,
RA Mohammed S.;
RT "Toward a comprehensive characterization of a human cancer cell
RT phosphoproteome.";
RL J. Proteome Res. 12:260-271(2013).
CC -!- INTERACTION:
CC Q5T7W7; O95994: AGR2; NbExp=3; IntAct=EBI-8994397, EBI-712648;
CC Q5T7W7; Q9H2G9: BLZF1; NbExp=5; IntAct=EBI-8994397, EBI-2548012;
CC Q5T7W7; Q13422-7: IKZF1; NbExp=3; IntAct=EBI-8994397, EBI-11522367;
CC Q5T7W7; Q9UKT9: IKZF3; NbExp=10; IntAct=EBI-8994397, EBI-747204;
CC Q5T7W7; Q0VD86: INCA1; NbExp=3; IntAct=EBI-8994397, EBI-6509505;
CC Q5T7W7; Q9ULR0-1: ISY1; NbExp=3; IntAct=EBI-8994397, EBI-18398632;
CC Q5T7W7; Q86T90: KIAA1328; NbExp=3; IntAct=EBI-8994397, EBI-3437878;
CC Q5T7W7; Q96JM7-2: L3MBTL3; NbExp=3; IntAct=EBI-8994397, EBI-11985629;
CC Q5T7W7; Q969G2: LHX4; NbExp=3; IntAct=EBI-8994397, EBI-2865388;
CC Q5T7W7; Q6FHY5: MEOX2; NbExp=3; IntAct=EBI-8994397, EBI-16439278;
CC Q5T7W7; Q9NRD5: PICK1; NbExp=3; IntAct=EBI-8994397, EBI-79165;
CC Q5T7W7; Q04864: REL; NbExp=3; IntAct=EBI-8994397, EBI-307352;
CC Q5T7W7; Q04864-2: REL; NbExp=3; IntAct=EBI-8994397, EBI-10829018;
CC Q5T7W7; Q8WVD3: RNF138; NbExp=3; IntAct=EBI-8994397, EBI-749039;
CC Q5T7W7; Q9Y2D8: SSX2IP; NbExp=3; IntAct=EBI-8994397, EBI-2212028;
CC Q5T7W7; P15884-3: TCF4; NbExp=3; IntAct=EBI-8994397, EBI-13636688;
CC Q5T7W7; Q8N1B4: VPS52; NbExp=6; IntAct=EBI-8994397, EBI-2799833;
CC Q5T7W7; O43829: ZBTB14; NbExp=3; IntAct=EBI-8994397, EBI-10176632;
CC Q5T7W7; O95159: ZFPL1; NbExp=3; IntAct=EBI-8994397, EBI-718439;
CC Q5T7W7; Q7Z4V0: ZNF438; NbExp=3; IntAct=EBI-8994397, EBI-11962468;
CC Q5T7W7; Q6NX45: ZNF774; NbExp=3; IntAct=EBI-8994397, EBI-10251462;
CC Q5T7W7; Q9UGI0: ZRANB1; NbExp=3; IntAct=EBI-8994397, EBI-527853;
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=1;
CC IsoId=Q5T7W7-1; Sequence=Displayed;
CC Name=2;
CC IsoId=Q5T7W7-2; Sequence=VSP_017825, VSP_017826, VSP_017827,
CC VSP_017828;
CC -!- SEQUENCE CAUTION:
CC Sequence=AAH35604.1; Type=Erroneous initiation; Evidence={ECO:0000305};
CC Sequence=BAB71188.1; Type=Erroneous initiation; Evidence={ECO:0000305};
CC Sequence=BAB71271.1; Type=Erroneous initiation; Evidence={ECO:0000305};
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DR EMBL; AF258575; AAG23778.1; -; mRNA.
DR EMBL; AK056453; BAB71188.1; ALT_INIT; mRNA.
DR EMBL; AK056752; BAB71271.1; ALT_INIT; mRNA.
DR EMBL; AK128615; BAC87530.1; -; mRNA.
DR EMBL; AK291199; BAF83888.1; -; mRNA.
DR EMBL; AL162385; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; CH471105; EAW58848.1; -; Genomic_DNA.
DR EMBL; BC035604; AAH35604.1; ALT_INIT; mRNA.
DR CCDS; CCDS6727.2; -. [Q5T7W7-1]
DR RefSeq; NP_640339.4; NM_139246.4. [Q5T7W7-1]
DR AlphaFoldDB; Q5T7W7; -.
DR SMR; Q5T7W7; -.
DR BioGRID; 127681; 52.
DR IntAct; Q5T7W7; 36.
DR MINT; Q5T7W7; -.
DR STRING; 9606.ENSP00000342499; -.
DR iPTMnet; Q5T7W7; -.
DR PhosphoSitePlus; Q5T7W7; -.
DR BioMuta; TSTD2; -.
DR DMDM; 74745447; -.
DR EPD; Q5T7W7; -.
DR jPOST; Q5T7W7; -.
DR MassIVE; Q5T7W7; -.
DR MaxQB; Q5T7W7; -.
DR PaxDb; Q5T7W7; -.
DR PeptideAtlas; Q5T7W7; -.
DR PRIDE; Q5T7W7; -.
DR ProteomicsDB; 64695; -. [Q5T7W7-1]
DR ProteomicsDB; 64696; -. [Q5T7W7-2]
DR Antibodypedia; 14352; 28 antibodies from 9 providers.
DR DNASU; 158427; -.
DR Ensembl; ENST00000341170.5; ENSP00000342499.4; ENSG00000136925.15. [Q5T7W7-1]
DR GeneID; 158427; -.
DR KEGG; hsa:158427; -.
DR MANE-Select; ENST00000341170.5; ENSP00000342499.4; NM_139246.5; NP_640339.4.
DR UCSC; uc004axn.4; human. [Q5T7W7-1]
DR CTD; 158427; -.
DR GeneCards; TSTD2; -.
DR HGNC; HGNC:30087; TSTD2.
DR HPA; ENSG00000136925; Low tissue specificity.
DR neXtProt; NX_Q5T7W7; -.
DR OpenTargets; ENSG00000136925; -.
DR PharmGKB; PA165586365; -.
DR VEuPathDB; HostDB:ENSG00000136925; -.
DR eggNOG; ENOG502QSQK; Eukaryota.
DR GeneTree; ENSGT00390000016307; -.
DR HOGENOM; CLU_038878_1_0_1; -.
DR InParanoid; Q5T7W7; -.
DR OMA; ECKEKLW; -.
DR OrthoDB; 1276106at2759; -.
DR PhylomeDB; Q5T7W7; -.
DR TreeFam; TF324329; -.
DR PathwayCommons; Q5T7W7; -.
DR SignaLink; Q5T7W7; -.
DR BioGRID-ORCS; 158427; 10 hits in 1075 CRISPR screens.
DR ChiTaRS; TSTD2; human.
DR GenomeRNAi; 158427; -.
DR Pharos; Q5T7W7; Tdark.
DR PRO; PR:Q5T7W7; -.
DR Proteomes; UP000005640; Chromosome 9.
DR RNAct; Q5T7W7; protein.
DR Bgee; ENSG00000136925; Expressed in secondary oocyte and 201 other tissues.
DR ExpressionAtlas; Q5T7W7; baseline and differential.
DR Genevisible; Q5T7W7; HS.
DR Gene3D; 3.40.250.10; -; 1.
DR InterPro; IPR001763; Rhodanese-like_dom.
DR InterPro; IPR036873; Rhodanese-like_dom_sf.
DR InterPro; IPR022111; Rhodanese_C.
DR InterPro; IPR020936; TrhO.
DR InterPro; IPR040503; TRHO_N.
DR PANTHER; PTHR43268; PTHR43268; 1.
DR Pfam; PF00581; Rhodanese; 1.
DR Pfam; PF12368; Rhodanese_C; 1.
DR Pfam; PF17773; UPF0176_N; 1.
DR SMART; SM00450; RHOD; 1.
DR SUPFAM; SSF52821; SSF52821; 1.
DR PROSITE; PS50206; RHODANESE_3; 1.
PE 1: Evidence at protein level;
KW Alternative splicing; Phosphoprotein; Reference proteome.
FT CHAIN 1..516
FT /note="Thiosulfate sulfurtransferase/rhodanese-like domain-
FT containing protein 2"
FT /id="PRO_0000230673"
FT DOMAIN 301..396
FT /note="Rhodanese"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00173"
FT REGION 490..516
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT ACT_SITE 355
FT /note="Cysteine persulfide intermediate"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00173"
FT MOD_RES 269
FT /note="Phosphoserine"
FT /evidence="ECO:0007744|PubMed:23186163"
FT VAR_SEQ 1..260
FT /note="Missing (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:15498874"
FT /id="VSP_017825"
FT VAR_SEQ 261..278
FT /note="EEIVPMGISPKKISYKKP -> MDLCLADSSVSAPAPHRQ (in isoform
FT 2)"
FT /evidence="ECO:0000303|PubMed:15498874"
FT /id="VSP_017826"
FT VAR_SEQ 418..427
FT /note="ECSYCGARWD -> GRSAQAQSPN (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:15498874"
FT /id="VSP_017827"
FT VAR_SEQ 428..516
FT /note="Missing (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:15498874"
FT /id="VSP_017828"
FT VARIANT 83
FT /note="R -> Q (in dbSNP:rs2773347)"
FT /evidence="ECO:0000269|PubMed:14702039,
FT ECO:0000269|PubMed:15489334, ECO:0000269|Ref.4"
FT /id="VAR_050842"
FT VARIANT 109
FT /note="A -> D (in dbSNP:rs10817858)"
FT /id="VAR_050843"
FT CONFLICT 30
FT /note="L -> F (in Ref. 2; BAC87530)"
FT /evidence="ECO:0000305"
FT CONFLICT 304
FT /note="D -> N (in Ref. 2; BAB71188)"
FT /evidence="ECO:0000305"
FT CONFLICT 433
FT /note="S -> C (in Ref. 2; BAC87530)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 516 AA; 58263 MW; 8C39781FD73CDA9F CRC64;
MPSSTSPDQG DDLENCILRF SDLDLKDMSL INPSSSLKAE LDGSTKKKYS FAKKKAFALF
VKTKEVPTKR SFECKEKLWK CCRQLFTDQT SIHRHVATQH ADEIYHQTAS ILKQLAVTLS
TSKSLSSADE KNPLKECLPH SHDVSAWLPD ISCFNPDELI SGQGSEEGEV LLYYCYHDLE
DPQWICAWQT ALCQHLHLTG KIRIAAEGIN GTVGGSKLAT RLYVEVMLSF PLFKDDLCKD
DFKTSKGGAH CFPELRVGVF EEIVPMGISP KKISYKKPGI HLSPGEFHKE VEKFLSQANQ
EQSDTILLDC RNFYESKIGR FQGCLAPDIR KFSYFPSYVD KNLELFREKR VLMYCTGGIR
CERGSAYLKA KGVCKEVFQL KGGIHKYLEE FPDGFYKGKL FVFDERYALS YNSDVVSECS
YCGARWDQYK LCSTPQCRQL VLTCPACQGQ GFTACCVTCQ DKGSRKVSGP MQDSFKEECE
CTARRPRIPR ELLQHVRQPV SPEPGPDADE DGPVLM
