ID   TSTD_DICDI              Reviewed;         154 AA.
AC   C0HLF1;
DT   05-DEC-2018, integrated into UniProtKB/Swiss-Prot.
DT   05-DEC-2018, sequence version 1.
DT   25-MAY-2022, entry version 11.
DE   RecName: Full=TSET complex member tstD {ECO:0000305};
DE   AltName: Full=Protein TSPOON {ECO:0000303|PubMed:24867644};
GN   Name=tstD {ECO:0000303|PubMed:24867644}; ORFNames=DDB_G0350235;
OS   Dictyostelium discoideum (Slime mold).
OC   Eukaryota; Amoebozoa; Evosea; Eumycetozoa; Dictyostelia; Dictyosteliales;
OC   Dictyosteliaceae; Dictyostelium.
OX   NCBI_TaxID=44689;
RN   [1] {ECO:0000305}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=AX4;
RX   PubMed=15875012; DOI=10.1038/nature03481;
RA   Eichinger L., Pachebat J.A., Gloeckner G., Rajandream M.A., Sucgang R.,
RA   Berriman M., Song J., Olsen R., Szafranski K., Xu Q., Tunggal B.,
RA   Kummerfeld S., Madera M., Konfortov B.A., Rivero F., Bankier A.T.,
RA   Lehmann R., Hamlin N., Davies R., Gaudet P., Fey P., Pilcher K., Chen G.,
RA   Saunders D., Sodergren E.J., Davis P., Kerhornou A., Nie X., Hall N.,
RA   Anjard C., Hemphill L., Bason N., Farbrother P., Desany B., Just E.,
RA   Morio T., Rost R., Churcher C.M., Cooper J., Haydock S., van Driessche N.,
RA   Cronin A., Goodhead I., Muzny D.M., Mourier T., Pain A., Lu M., Harper D.,
RA   Lindsay R., Hauser H., James K.D., Quiles M., Madan Babu M., Saito T.,
RA   Buchrieser C., Wardroper A., Felder M., Thangavelu M., Johnson D.,
RA   Knights A., Loulseged H., Mungall K.L., Oliver K., Price C., Quail M.A.,
RA   Urushihara H., Hernandez J., Rabbinowitsch E., Steffen D., Sanders M.,
RA   Ma J., Kohara Y., Sharp S., Simmonds M.N., Spiegler S., Tivey A.,
RA   Sugano S., White B., Walker D., Woodward J.R., Winckler T., Tanaka Y.,
RA   Shaulsky G., Schleicher M., Weinstock G.M., Rosenthal A., Cox E.C.,
RA   Chisholm R.L., Gibbs R.A., Loomis W.F., Platzer M., Kay R.R.,
RA   Williams J.G., Dear P.H., Noegel A.A., Barrell B.G., Kuspa A.;
RT   "The genome of the social amoeba Dictyostelium discoideum.";
RL   Nature 435:43-57(2005).
RN   [2] {ECO:0000305}
RP   IDENTIFICATION IN THE TSET COMPLEX, SUBCELLULAR LOCATION, DISRUPTION
RP   PHENOTYPE, AND IDENTIFICATION BY MASS SPECTROMETRY.
RX   PubMed=24867644; DOI=10.7554/elife.02866;
RA   Hirst J., Schlacht A., Norcott J.P., Traynor D., Bloomfield G.,
RA   Antrobus R., Kay R.R., Dacks J.B., Robinson M.S.;
RT   "Characterization of TSET, an ancient and widespread membrane trafficking
RT   complex.";
RL   Elife 3:E02866-E02866(2014).
CC   -!- SUBUNIT: Component of the TSET complex, a heterohexamer composed of
CC       tstA, tstB, tstC, tstD, tstE and tstF, which may act in plasma membrane
CC       turnover. tstA, tstB, tstC and tstD are likely to be the core complex
CC       members with tstE and tstF acting as associated scaffold proteins.
CC       {ECO:0000269|PubMed:24867644}.
CC   -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000269|PubMed:24867644};
CC       Peripheral membrane protein {ECO:0000305}. Cytoplasm
CC       {ECO:0000269|PubMed:24867644}.
CC   -!- DISRUPTION PHENOTYPE: Knockout cells grow at least as fast as a control
CC       axenic strain and differentiation also appears normal with fruiting
CC       bodies forming under appropriate stimuli. Uptake of FITC-dextran, an
CC       assay for fluid phase endocytosis, is unimpaired while uptake of FM1-
CC       43, a membrane marker, is slower than in the control.
CC       {ECO:0000269|PubMed:24867644}.
CC   -!- SIMILARITY: Belongs to the adaptor complexes small subunit family.
CC       {ECO:0000305}.
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DR   EMBL; AAFI02000005; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR   AlphaFoldDB; C0HLF1; -.
DR   SMR; C0HLF1; -.
DR   dictyBase; DDB_G0350235; tstD.
DR   PRO; PR:C0HLF1; -.
DR   Proteomes; UP000002195; Chromosome 1.
DR   GO; GO:0030126; C:COPI vesicle coat; IBA:GO_Central.
DR   GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0006891; P:intra-Golgi vesicle-mediated transport; IBA:GO_Central.
DR   GO; GO:0006886; P:intracellular protein transport; IBA:GO_Central.
DR   GO; GO:0060098; P:membrane reorganization involved in phagocytosis, engulfment; IMP:dictyBase.
DR   GO; GO:0006890; P:retrograde vesicle-mediated transport, Golgi to endoplasmic reticulum; IBA:GO_Central.
DR   InterPro; IPR022775; AP_mu_sigma_su.
DR   InterPro; IPR039652; Coatomer_zeta.
DR   InterPro; IPR011012; Longin-like_dom_sf.
DR   PANTHER; PTHR11043; PTHR11043; 1.
DR   Pfam; PF01217; Clat_adaptor_s; 1.
DR   SUPFAM; SSF64356; SSF64356; 1.
PE   1: Evidence at protein level;
KW   Cell membrane; Cytoplasm; Membrane; Reference proteome.
FT   CHAIN           1..154
FT                   /note="TSET complex member tstD"
FT                   /id="PRO_0000445761"
SQ   SEQUENCE   154 AA;  17984 MW;  F3E761B63D9333B2 CRC64;
     MINSLLITNN NGNIVFSKYY SNINDEKQSE YEKLLYQFTK DEWVNSKNEK HLVTEFSGYI
     TVFTNVGDLM LFLCGSEEMD ELALSDVFFP IIESLKDICK KKGVTEQNFI EQIPKFILYL
     DEIIQRGHLD QVQLESIQNY SSLKHDQPIK KDNV