ID   TSX_KLEAK               Reviewed;         294 AA.
AC   P40785; G0E7G7;
DT   01-FEB-1995, integrated into UniProtKB/Swiss-Prot.
DT   14-DEC-2011, sequence version 2.
DT   03-AUG-2022, entry version 93.
DE   RecName: Full=Nucleoside-specific channel-forming protein Tsx {ECO:0000305};
DE   Flags: Precursor;
GN   Name=tsx {ECO:0000303|PubMed:9043137}; OrderedLocusNames=EAE_12525;
OS   Klebsiella aerogenes (strain ATCC 13048 / DSM 30053 / CCUG 1429 / JCM 1235
OS   / KCTC 2190 / NBRC 13534 / NCIMB 10102 / NCTC 10006 / CDC 819-56)
OS   (Enterobacter aerogenes).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC   Enterobacteriaceae; Klebsiella/Raoultella group; Klebsiella.
OX   NCBI_TaxID=1028307;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, AND SUBCELLULAR LOCATION.
RC   STRAIN=ATCC 13048 / DSM 30053 / CCUG 1429 / JCM 1235 / KCTC 2190 / NBRC
RC   13534 / NCIMB 10102 / NCTC 10006 / CDC 819-56;
RX   PubMed=9043137; DOI=10.1099/00221287-143-2-603;
RA   Nieweg A., Bremer E.;
RT   "The nucleoside-specific Tsx channel from the outer membrane of Salmonella
RT   typhimurium, Klebsiella pneumoniae and Enterobacter aerogenes: functional
RT   characterization and DNA sequence analysis of the tsx genes.";
RL   Microbiology 143:603-615(1997).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 13048 / DSM 30053 / CCUG 1429 / JCM 1235 / KCTC 2190 / NBRC
RC   13534 / NCIMB 10102 / NCTC 10006 / CDC 819-56;
RX   PubMed=22493190; DOI=10.1128/jb.00028-12;
RA   Shin S.H., Kim S., Kim J.Y., Lee S., Um Y., Oh M.K., Kim Y.R., Lee J.,
RA   Yang K.S.;
RT   "Complete genome sequence of Enterobacter aerogenes KCTC 2190.";
RL   J. Bacteriol. 194:2373-2374(2012).
CC   -!- FUNCTION: Functions as substrate-specific channel for nucleosides and
CC       deoxynucleosides. Functions also in albicidin uptake and as receptor
CC       for colicin K. Is also a receptor for several Tsx-specific
CC       bacteriophages. {ECO:0000269|PubMed:9043137}.
CC   -!- SUBCELLULAR LOCATION: Cell outer membrane {ECO:0000269|PubMed:9043137};
CC       Multi-pass membrane protein {ECO:0000305|PubMed:9043137}.
CC   -!- SIMILARITY: Belongs to the nucleoside-specific channel-forming outer
CC       membrane porin (Tsx) (TC 1.B.10) family. {ECO:0000305}.
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DR   EMBL; Z26655; CAA81396.1; -; Genomic_DNA.
DR   EMBL; CP002824; AEG97419.1; -; Genomic_DNA.
DR   PIR; S49142; S49142.
DR   RefSeq; WP_015367996.1; NC_015663.1.
DR   RefSeq; YP_004592698.1; NC_015663.1.
DR   AlphaFoldDB; P40785; -.
DR   SMR; P40785; -.
DR   STRING; 1028307.EAE_12525; -.
DR   PRIDE; P40785; -.
DR   EnsemblBacteria; AEG97419; AEG97419; EAE_12525.
DR   GeneID; 66605202; -.
DR   KEGG; eae:EAE_12525; -.
DR   PATRIC; fig|1028307.3.peg.2498; -.
DR   eggNOG; COG3248; Bacteria.
DR   HOGENOM; CLU_073836_0_0_6; -.
DR   OMA; KSTGWGY; -.
DR   Proteomes; UP000008881; Chromosome.
DR   GO; GO:0009279; C:cell outer membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0046930; C:pore complex; IEA:UniProtKB-KW.
DR   GO; GO:0005337; F:nucleoside transmembrane transporter activity; IEA:InterPro.
DR   GO; GO:0015288; F:porin activity; IEA:UniProtKB-KW.
DR   GO; GO:0006811; P:ion transport; IEA:UniProtKB-KW.
DR   Gene3D; 2.40.230.20; -; 1.
DR   InterPro; IPR003055; Channel_Tsx.
DR   InterPro; IPR018013; Channel_Tsx-like.
DR   InterPro; IPR036777; Channel_Tsx-like_sf.
DR   Pfam; PF03502; Channel_Tsx; 1.
DR   PRINTS; PR01277; CHANNELTSX.
DR   SUPFAM; SSF111364; SSF111364; 1.
PE   3: Inferred from homology;
KW   Cell outer membrane; Ion transport; Membrane; Porin; Signal; Transmembrane;
KW   Transmembrane beta strand; Transport.
FT   SIGNAL          1..22
FT                   /evidence="ECO:0000255"
FT   CHAIN           23..294
FT                   /note="Nucleoside-specific channel-forming protein Tsx"
FT                   /id="PRO_0000025192"
FT   CONFLICT        184
FT                   /note="G -> A (in Ref. 1; CAA81396)"
FT                   /evidence="ECO:0000305"
SQ   SEQUENCE   294 AA;  33574 MW;  FB1B03D7A1127279 CRC64;
     MKKTLLAASA VVALSASFTA GAAETEKPQY LSDWWHQSVN VVGSYHTRFG PQIRNDTYLE
     YEAFAKKDWF DFYGYIDAPV FFGGNSTAKG IWNKGSPLFM EIEPRFSIDK LTNTDLSFGP
     FKEWYFANNY IYDMGRNDSQ EQSTWYMGLG TDIDTGLPMS LSLNIYAKYQ WQNYGASNEN
     EWDGYRFKVK YFVPLTDLWG GSLSYIGFTN FDWGSDLGDD NFYDMNGKHA RTSNSIASSH
     ILALNYAHWH YSIVARYFHN GGQWADDAKL NFGDGDFSVR STGWGGYFVV GYNF