TSYL5_HUMAN
ID TSYL5_HUMAN Reviewed; 417 AA.
AC Q86VY4; B3KRF0; Q9C0B3;
DT 23-OCT-2007, integrated into UniProtKB/Swiss-Prot.
DT 23-OCT-2007, sequence version 2.
DT 03-AUG-2022, entry version 122.
DE RecName: Full=Testis-specific Y-encoded-like protein 5;
DE Short=TSPY-like protein 5;
GN Name=TSPYL5; Synonyms=KIAA1750;
OS Homo sapiens (Human).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae;
OC Homo.
OX NCBI_TaxID=9606;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC TISSUE=Brain;
RX PubMed=11214970; DOI=10.1093/dnares/7.6.347;
RA Nagase T., Kikuno R., Hattori A., Kondo Y., Okumura K., Ohara O.;
RT "Prediction of the coding sequences of unidentified human genes. XIX. The
RT complete sequences of 100 new cDNA clones from brain which code for large
RT proteins in vitro.";
RL DNA Res. 7:347-355(2000).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC TISSUE=Brain;
RX PubMed=14702039; DOI=10.1038/ng1285;
RA Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R.,
RA Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H.,
RA Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.,
RA Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K.,
RA Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H.,
RA Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M.,
RA Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K.,
RA Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T.,
RA Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M.,
RA Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S.,
RA Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H.,
RA Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K.,
RA Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N.,
RA Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S.,
RA Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O.,
RA Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H.,
RA Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B.,
RA Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y.,
RA Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K.,
RA Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T.,
RA Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T.,
RA Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y.,
RA Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H.,
RA Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y.,
RA Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H.,
RA Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O.,
RA Isogai T., Sugano S.;
RT "Complete sequencing and characterization of 21,243 full-length human
RT cDNAs.";
RL Nat. Genet. 36:40-45(2004).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA], AND VARIANT VAL-365.
RC TISSUE=Testis;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [4]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Cervix carcinoma;
RX PubMed=18669648; DOI=10.1073/pnas.0805139105;
RA Dephoure N., Zhou C., Villen J., Beausoleil S.A., Bakalarski C.E.,
RA Elledge S.J., Gygi S.P.;
RT "A quantitative atlas of mitotic phosphorylation.";
RL Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008).
RN [5]
RP FUNCTION.
RX PubMed=20079711; DOI=10.1016/j.bbrc.2010.01.045;
RA Kim E.J., Lee S.Y., Kim T.R., Choi S.I., Cho E.W., Kim K.C., Kim I.G.;
RT "TSPYL5 is involved in cell growth and the resistance to radiation in A549
RT cells via the regulation of p21(WAF1/Cip1) and PTEN/AKT pathway.";
RL Biochem. Biophys. Res. Commun. 392:448-453(2010).
RN [6]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RX PubMed=21269460; DOI=10.1186/1752-0509-5-17;
RA Burkard T.R., Planyavsky M., Kaupe I., Breitwieser F.P., Buerckstuemmer T.,
RA Bennett K.L., Superti-Furga G., Colinge J.;
RT "Initial characterization of the human central proteome.";
RL BMC Syst. Biol. 5:17-17(2011).
RN [7]
RP FUNCTION, AND INTERACTION WITH USP7.
RX PubMed=21170034; DOI=10.1038/ncb2142;
RA Epping M.T., Meijer L.A., Krijgsman O., Bos J.L., Pandolfi P.P.,
RA Bernards R.;
RT "TSPYL5 suppresses p53 levels and function by physical interaction with
RT USP7.";
RL Nat. Cell Biol. 13:102-108(2011).
CC -!- FUNCTION: Involved in modulation of cell growth and cellular response
CC to gamma radiation probably via regulation of the Akt signaling
CC pathway. Involved in regulation of p53/TP53. Suppresses p53/TP53
CC protein levels and promotes its ubiquitination; the function is
CC dependent on USP7 and independent on MDM2. Proposed to displace
CC p53/TP53 from interaction with USP7. {ECO:0000269|PubMed:20079711,
CC ECO:0000269|PubMed:21170034}.
CC -!- SUBUNIT: Interacts with USP7. {ECO:0000269|PubMed:21170034}.
CC -!- INTERACTION:
CC Q86VY4; Q93009: USP7; NbExp=4; IntAct=EBI-3436472, EBI-302474;
CC -!- SIMILARITY: Belongs to the nucleosome assembly protein (NAP) family.
CC {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=BAB21841.1; Type=Erroneous initiation; Note=Extended N-terminus.; Evidence={ECO:0000305};
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DR EMBL; AB051537; BAB21841.1; ALT_INIT; mRNA.
DR EMBL; AK091445; BAG52362.1; -; mRNA.
DR EMBL; BC045630; AAH45630.1; -; mRNA.
DR CCDS; CCDS34927.1; -.
DR RefSeq; NP_277047.2; NM_033512.2.
DR AlphaFoldDB; Q86VY4; -.
DR SMR; Q86VY4; -.
DR BioGRID; 124538; 31.
DR CORUM; Q86VY4; -.
DR IntAct; Q86VY4; 27.
DR MINT; Q86VY4; -.
DR STRING; 9606.ENSP00000322802; -.
DR iPTMnet; Q86VY4; -.
DR MetOSite; Q86VY4; -.
DR PhosphoSitePlus; Q86VY4; -.
DR BioMuta; TSPYL5; -.
DR DMDM; 160198165; -.
DR EPD; Q86VY4; -.
DR jPOST; Q86VY4; -.
DR MassIVE; Q86VY4; -.
DR MaxQB; Q86VY4; -.
DR PaxDb; Q86VY4; -.
DR PeptideAtlas; Q86VY4; -.
DR PRIDE; Q86VY4; -.
DR ProteomicsDB; 70090; -.
DR Antibodypedia; 26007; 91 antibodies from 18 providers.
DR DNASU; 85453; -.
DR Ensembl; ENST00000322128.5; ENSP00000322802.3; ENSG00000180543.5.
DR GeneID; 85453; -.
DR KEGG; hsa:85453; -.
DR MANE-Select; ENST00000322128.5; ENSP00000322802.3; NM_033512.3; NP_277047.2.
DR UCSC; uc003yhy.4; human.
DR CTD; 85453; -.
DR DisGeNET; 85453; -.
DR GeneCards; TSPYL5; -.
DR HGNC; HGNC:29367; TSPYL5.
DR HPA; ENSG00000180543; Tissue enhanced (testis).
DR MIM; 614721; gene.
DR neXtProt; NX_Q86VY4; -.
DR OpenTargets; ENSG00000180543; -.
DR PharmGKB; PA128394738; -.
DR VEuPathDB; HostDB:ENSG00000180543; -.
DR eggNOG; KOG1508; Eukaryota.
DR GeneTree; ENSGT00940000162862; -.
DR HOGENOM; CLU_051687_2_1_1; -.
DR InParanoid; Q86VY4; -.
DR OMA; APETCST; -.
DR OrthoDB; 1191764at2759; -.
DR PhylomeDB; Q86VY4; -.
DR TreeFam; TF313386; -.
DR PathwayCommons; Q86VY4; -.
DR SignaLink; Q86VY4; -.
DR BioGRID-ORCS; 85453; 334 hits in 1071 CRISPR screens.
DR GenomeRNAi; 85453; -.
DR Pharos; Q86VY4; Tbio.
DR PRO; PR:Q86VY4; -.
DR Proteomes; UP000005640; Chromosome 8.
DR RNAct; Q86VY4; protein.
DR Bgee; ENSG00000180543; Expressed in adult organism and 208 other tissues.
DR Genevisible; Q86VY4; HS.
DR GO; GO:0000785; C:chromatin; IBA:GO_Central.
DR GO; GO:0005634; C:nucleus; IBA:GO_Central.
DR GO; GO:0003682; F:chromatin binding; IBA:GO_Central.
DR GO; GO:0042393; F:histone binding; IBA:GO_Central.
DR GO; GO:0071480; P:cellular response to gamma radiation; IMP:UniProtKB.
DR GO; GO:0006334; P:nucleosome assembly; IEA:InterPro.
DR GO; GO:0008284; P:positive regulation of cell population proliferation; IDA:UniProtKB.
DR GO; GO:0051897; P:positive regulation of protein kinase B signaling; IMP:UniProtKB.
DR GO; GO:0031398; P:positive regulation of protein ubiquitination; IDA:UniProtKB.
DR GO; GO:0040008; P:regulation of growth; IEA:UniProtKB-KW.
DR InterPro; IPR037231; NAP-like_sf.
DR InterPro; IPR002164; NAP_family.
DR PANTHER; PTHR11875; PTHR11875; 1.
DR Pfam; PF00956; NAP; 1.
DR SUPFAM; SSF143113; SSF143113; 1.
PE 1: Evidence at protein level;
KW Growth regulation; Reference proteome.
FT CHAIN 1..417
FT /note="Testis-specific Y-encoded-like protein 5"
FT /id="PRO_0000307277"
FT REGION 1..55
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 93..112
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 127..202
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 391..417
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1..22
FT /note="Basic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 403..417
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT VARIANT 120
FT /note="T -> S (in dbSNP:rs2635164)"
FT /id="VAR_035393"
FT VARIANT 365
FT /note="I -> V (in dbSNP:rs17854366)"
FT /evidence="ECO:0000269|PubMed:15489334"
FT /id="VAR_035394"
SQ SEQUENCE 417 AA; 45143 MW; 920E123167C08829 CRC64;
MSGRSRGRKS SRAKNRGKGR AKARVRPAPD DAPRDPDPSQ YQSLGEDTQA AQVQAGAGWG
GLEAAASAQL LRLGEEAACR LPLDCGLALR ARAAGDHGQA AARPGPGKAA SLSERLAADT
VFVGTAGTVG RPKNAPRVGN RRGPAGKKAP ETCSTAGRGP QVIAGGRQKK GAAGENTSVS
AGEEKKEERD AGSGPPATEG SMDTLENVQL KLENMNAQAD RAYLRLSRKF GQLRLQHLER
RNHLIQNIPG FWGQAFQNHP QLASFLNSQE KEVLSYLNSL EVEELGLARL GYKIKFYFDR
NPYFQNKVLI KEYGCGPSGQ VVSRSTPIQW LPGHDLQSLS QGNPENNRSF FGWFSNHSSI
ESDKIVEIIN EELWPNPLQF YLLSEGARVE KGKEKEGRQG PGKQPMETTQ PGVSQSN
