TT2_ARATH
ID TT2_ARATH Reviewed; 258 AA.
AC Q9FJA2; A0MFJ2; Q53YP9;
DT 13-DEC-2002, integrated into UniProtKB/Swiss-Prot.
DT 01-MAR-2001, sequence version 1.
DT 03-AUG-2022, entry version 152.
DE RecName: Full=Transcription factor TT2;
DE AltName: Full=Myb-related protein 123;
DE Short=AtMYB123;
DE AltName: Full=Myb-related transcription factor LBM2-like;
DE AltName: Full=Protein TRANSPARENT TESTA 2;
GN Name=TT2; Synonyms=MYB123; OrderedLocusNames=At5g35550; ORFNames=MOK9.18;
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND MUTANTS TT2-2 AND TT2-4.
RC STRAIN=cv. Columbia, cv. Landsberg erecta, and cv. Wassilewskija;
RX PubMed=11549766; DOI=10.2307/3871430;
RA Nesi N., Jond C., Debeaujon I., Caboche M., Lepiniec L.;
RT "The Arabidopsis TT2 gene encodes an R2R3 MYB domain protein that acts as a
RT key determinant for proanthocyanidin accumulation in developing seed.";
RL Plant Cell 13:2099-2114(2001).
RN [2]
RP NUCLEOTIDE SEQUENCE [MRNA].
RC STRAIN=cv. Columbia;
RX PubMed=11597504; DOI=10.1016/s1369-5266(00)00199-0;
RA Stracke R., Werber M., Weisshaar B.;
RT "The R2R3-MYB gene family in Arabidopsis thaliana.";
RL Curr. Opin. Plant Biol. 4:447-456(2001).
RN [3]
RP NUCLEOTIDE SEQUENCE [MRNA].
RA Qu L.-J., Gu H.;
RT "The MYB transcription factor family in Arabidopsis: a genome-wide cloning
RT and expression pattern analysis.";
RL Submitted (JAN-2004) to the EMBL/GenBank/DDBJ databases.
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=9872454; DOI=10.1093/dnares/5.5.297;
RA Nakamura Y., Sato S., Asamizu E., Kaneko T., Kotani H., Miyajima N.,
RA Tabata S.;
RT "Structural analysis of Arabidopsis thaliana chromosome 5. VII. Sequence
RT features of the regions of 1,013,767 bp covered by sixteen physically
RT assigned P1 and TAC clones.";
RL DNA Res. 5:297-308(1998).
RN [5]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [6]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RX PubMed=17147637; DOI=10.1111/j.1467-7652.2006.00183.x;
RA Underwood B.A., Vanderhaeghen R., Whitford R., Town C.D., Hilson P.;
RT "Simultaneous high-throughput recombinational cloning of open reading
RT frames in closed and open configurations.";
RL Plant Biotechnol. J. 4:317-324(2006).
RN [7]
RP FUNCTION, AND INTERACTION WITH BHLH2; BHLH12 AND BHLH42.
RX PubMed=15361138; DOI=10.1111/j.1365-313x.2004.02183.x;
RA Zimmermann I.M., Heim M.A., Weisshaar B., Uhrig J.F.;
RT "Comprehensive identification of Arabidopsis thaliana MYB transcription
RT factors interacting with R/B-like BHLH proteins.";
RL Plant J. 40:22-34(2004).
RN [8]
RP GENE FAMILY.
RX PubMed=16463103; DOI=10.1007/s11103-005-2910-y;
RA Chen Y., Yang X., He K., Liu M., Li J., Gao Z., Lin Z., Zhang Y., Wang X.,
RA Qiu X., Shen Y., Zhang L., Deng X., Luo J., Deng X.-W., Chen Z., Gu H.,
RA Qu L.-J.;
RT "The MYB transcription factor superfamily of Arabidopsis: expression
RT analysis and phylogenetic comparison with the rice MYB family.";
RL Plant Mol. Biol. 60:107-124(2006).
CC -!- FUNCTION: Transcription activator, when associated with
CC BHLH2/EGL3/MYC146, BHLH12/MYC1, or BHLH42/TT8. Involved in the control
CC of flavonoid late metabolism in developing siliques. Plays a key role
CC in determining the tissue-specific activation of leucoanthocyanidin
CC reductase (BANYULS). {ECO:0000269|PubMed:15361138}.
CC -!- SUBUNIT: Interacts with BHLH2/EGL3/MYC146, BHLH12/MYC1 and BHLH42/TT8.
CC {ECO:0000269|PubMed:15361138}.
CC -!- INTERACTION:
CC Q9FJA2; Q9FT81: TT8; NbExp=5; IntAct=EBI-395778, EBI-395790;
CC -!- SUBCELLULAR LOCATION: Nucleus.
CC -!- TISSUE SPECIFICITY: Expressed at a high level in immature siliques and
CC at a lower level in flowers. Undetected in young seedlings, roots,
CC leaves and inflorescence stems.
CC -!- DEVELOPMENTAL STAGE: Highly expressed from the very early stages of
CC embryogenesis to the globular stage, decreases rapidly from the late
CC heart-torpedo stage and did not persist after the completion of
CC embryogenesis.
CC -!- MISCELLANEOUS: TT2 activity is tightly linked to the presence of TT8.
CC -!- SEQUENCE CAUTION:
CC Sequence=ABK28720.1; Type=Erroneous termination; Note=Extended C-terminus.; Evidence={ECO:0000305};
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DR EMBL; AJ299452; CAC40021.1; -; Genomic_DNA.
DR EMBL; AF371981; AAK54744.1; -; mRNA.
DR EMBL; AY519630; AAS10100.1; -; mRNA.
DR EMBL; AB015477; BAB08716.1; -; Genomic_DNA.
DR EMBL; CP002688; AED93980.1; -; Genomic_DNA.
DR EMBL; DQ447000; ABE66191.1; -; mRNA.
DR EMBL; DQ653318; ABK28720.1; ALT_SEQ; mRNA.
DR RefSeq; NP_198405.1; NM_122946.3.
DR AlphaFoldDB; Q9FJA2; -.
DR SMR; Q9FJA2; -.
DR BioGRID; 18773; 22.
DR IntAct; Q9FJA2; 16.
DR STRING; 3702.AT5G35550.1; -.
DR iPTMnet; Q9FJA2; -.
DR PaxDb; Q9FJA2; -.
DR PRIDE; Q9FJA2; -.
DR ProteomicsDB; 234653; -.
DR EnsemblPlants; AT5G35550.1; AT5G35550.1; AT5G35550.
DR GeneID; 833520; -.
DR Gramene; AT5G35550.1; AT5G35550.1; AT5G35550.
DR KEGG; ath:AT5G35550; -.
DR Araport; AT5G35550; -.
DR TAIR; locus:2169538; AT5G35550.
DR eggNOG; KOG0048; Eukaryota.
DR HOGENOM; CLU_028567_6_7_1; -.
DR InParanoid; Q9FJA2; -.
DR OMA; AWTDHED; -.
DR PhylomeDB; Q9FJA2; -.
DR PRO; PR:Q9FJA2; -.
DR Proteomes; UP000006548; Chromosome 5.
DR ExpressionAtlas; Q9FJA2; baseline and differential.
DR Genevisible; Q9FJA2; AT.
DR GO; GO:0005634; C:nucleus; IDA:TAIR.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR GO; GO:0003700; F:DNA-binding transcription factor activity; ISS:TAIR.
DR GO; GO:0000976; F:transcription cis-regulatory region binding; IBA:GO_Central.
DR GO; GO:0030154; P:cell differentiation; IBA:GO_Central.
DR GO; GO:0006633; P:fatty acid biosynthetic process; IMP:TAIR.
DR GO; GO:0009813; P:flavonoid biosynthetic process; IEA:UniProtKB-KW.
DR GO; GO:0010023; P:proanthocyanidin biosynthetic process; IMP:TAIR.
DR GO; GO:0006970; P:response to osmotic stress; IMP:TAIR.
DR CDD; cd00167; SANT; 2.
DR InterPro; IPR009057; Homeobox-like_sf.
DR InterPro; IPR017930; Myb_dom.
DR InterPro; IPR001005; SANT/Myb.
DR Pfam; PF00249; Myb_DNA-binding; 2.
DR SMART; SM00717; SANT; 2.
DR SUPFAM; SSF46689; SSF46689; 1.
DR PROSITE; PS51294; HTH_MYB; 2.
PE 1: Evidence at protein level;
KW Activator; ATP-binding; DNA-binding; Flavonoid biosynthesis;
KW Nucleotide-binding; Nucleus; Reference proteome; Repeat; Transcription;
KW Transcription regulation.
FT CHAIN 1..258
FT /note="Transcription factor TT2"
FT /id="PRO_0000197078"
FT DOMAIN 11..63
FT /note="HTH myb-type 1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00625"
FT DOMAIN 64..118
FT /note="HTH myb-type 2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00625"
FT DNA_BIND 39..63
FT /note="H-T-H motif"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00625"
FT DNA_BIND 91..114
FT /note="H-T-H motif"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00625"
FT BINDING 47..54
FT /ligand="ATP"
FT /ligand_id="ChEBI:CHEBI:30616"
FT /evidence="ECO:0000255"
FT VARIANT 174
FT /note="Q -> L (in strain: cv. Wassilewskija)"
FT MUTAGEN 66
FT /note="G->R: In tt2-4; loss of seed pigmentation."
FT MUTAGEN 127..258
FT /note="Missing: In tt2-2; reduced seed pigmentation."
SQ SEQUENCE 258 AA; 29611 MW; 3BA9CB3F723D1C2E CRC64;
MGKRATTSVR REELNRGAWT DHEDKILRDY ITTHGEGKWS TLPNQAGLKR CGKSCRLRWK
NYLRPGIKRG NISSDEEELI IRLHNLLGNR WSLIAGRLPG RTDNEIKNHW NSNLRKRLPK
TQTKQPKRIK HSTNNENNVC VIRTKAIRCS KTLLFSDLSL QKKSSTSPLP LKEQEMDQGG
SSLMGDLEFD FDRIHSEFHF PDLMDFDGLD CGNVTSLVSS NEILGELVPA QGNLDLNRPF
TSCHHRGDDE DWLRDFTC
