ID   TT30A_DANRE             Reviewed;         651 AA.
AC   A7YE96; A8CVX5; B3DKN4;
DT   20-MAY-2008, integrated into UniProtKB/Swiss-Prot.
DT   20-MAY-2008, sequence version 2.
DT   03-AUG-2022, entry version 92.
DE   RecName: Full=Tetratricopeptide repeat protein 30A;
DE            Short=TPR repeat protein 30A;
DE   AltName: Full=Protein fleer;
GN   Name=ttc30a; Synonyms=flr;
OS   Danio rerio (Zebrafish) (Brachydanio rerio).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC   Actinopterygii; Neopterygii; Teleostei; Ostariophysi; Cypriniformes;
OC   Danionidae; Danioninae; Danio.
OX   NCBI_TaxID=7955;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 1 AND 2), FUNCTION, SUBCELLULAR
RP   LOCATION, TISSUE SPECIFICITY, AND DEVELOPMENTAL STAGE.
RX   PubMed=17761526; DOI=10.1091/mbc.e07-06-0537;
RA   Pathak N.H., Obara T., Mangos S., Liu Y., Drummond I.A.;
RT   "The zebrafish fleer gene encodes an essential regulator of cilia tubulin
RT   polyglutamylation.";
RL   Mol. Biol. Cell 18:4353-4364(2007).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=Tuebingen;
RX   PubMed=23594743; DOI=10.1038/nature12111;
RA   Howe K., Clark M.D., Torroja C.F., Torrance J., Berthelot C., Muffato M.,
RA   Collins J.E., Humphray S., McLaren K., Matthews L., McLaren S., Sealy I.,
RA   Caccamo M., Churcher C., Scott C., Barrett J.C., Koch R., Rauch G.J.,
RA   White S., Chow W., Kilian B., Quintais L.T., Guerra-Assuncao J.A., Zhou Y.,
RA   Gu Y., Yen J., Vogel J.H., Eyre T., Redmond S., Banerjee R., Chi J., Fu B.,
RA   Langley E., Maguire S.F., Laird G.K., Lloyd D., Kenyon E., Donaldson S.,
RA   Sehra H., Almeida-King J., Loveland J., Trevanion S., Jones M., Quail M.,
RA   Willey D., Hunt A., Burton J., Sims S., McLay K., Plumb B., Davis J.,
RA   Clee C., Oliver K., Clark R., Riddle C., Elliot D., Threadgold G.,
RA   Harden G., Ware D., Begum S., Mortimore B., Kerry G., Heath P.,
RA   Phillimore B., Tracey A., Corby N., Dunn M., Johnson C., Wood J., Clark S.,
RA   Pelan S., Griffiths G., Smith M., Glithero R., Howden P., Barker N.,
RA   Lloyd C., Stevens C., Harley J., Holt K., Panagiotidis G., Lovell J.,
RA   Beasley H., Henderson C., Gordon D., Auger K., Wright D., Collins J.,
RA   Raisen C., Dyer L., Leung K., Robertson L., Ambridge K., Leongamornlert D.,
RA   McGuire S., Gilderthorp R., Griffiths C., Manthravadi D., Nichol S.,
RA   Barker G., Whitehead S., Kay M., Brown J., Murnane C., Gray E.,
RA   Humphries M., Sycamore N., Barker D., Saunders D., Wallis J., Babbage A.,
RA   Hammond S., Mashreghi-Mohammadi M., Barr L., Martin S., Wray P.,
RA   Ellington A., Matthews N., Ellwood M., Woodmansey R., Clark G., Cooper J.,
RA   Tromans A., Grafham D., Skuce C., Pandian R., Andrews R., Harrison E.,
RA   Kimberley A., Garnett J., Fosker N., Hall R., Garner P., Kelly D., Bird C.,
RA   Palmer S., Gehring I., Berger A., Dooley C.M., Ersan-Urun Z., Eser C.,
RA   Geiger H., Geisler M., Karotki L., Kirn A., Konantz J., Konantz M.,
RA   Oberlander M., Rudolph-Geiger S., Teucke M., Lanz C., Raddatz G.,
RA   Osoegawa K., Zhu B., Rapp A., Widaa S., Langford C., Yang F.,
RA   Schuster S.C., Carter N.P., Harrow J., Ning Z., Herrero J., Searle S.M.,
RA   Enright A., Geisler R., Plasterk R.H., Lee C., Westerfield M.,
RA   de Jong P.J., Zon L.I., Postlethwait J.H., Nusslein-Volhard C.,
RA   Hubbard T.J., Roest Crollius H., Rogers J., Stemple D.L.;
RT   "The zebrafish reference genome sequence and its relationship to the human
RT   genome.";
RL   Nature 496:498-503(2013).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
RG   NIH - Zebrafish Gene Collection (ZGC) project;
RL   Submitted (APR-2008) to the EMBL/GenBank/DDBJ databases.
CC   -!- FUNCTION: Plays a role in anterograde intraflagellar transport (IFT),
CC       the process by which cilia precursors are transported from the base of
CC       the cilium to the site of their incorporation at the tip (By
CC       similarity). Required for polyglutamylation of axonemal tubulin, which
CC       is a prerequisite for correct assembly of cilia and for normal cilia
CC       beat amplitude. Does not seem to be required for neuronal microtubule
CC       polyglutamylation. {ECO:0000250, ECO:0000269|PubMed:17761526}.
CC   -!- SUBCELLULAR LOCATION: Cell projection, cilium
CC       {ECO:0000269|PubMed:17761526}.
CC   -!- ALTERNATIVE PRODUCTS:
CC       Event=Alternative splicing; Named isoforms=2;
CC       Name=1;
CC         IsoId=A7YE96-1; Sequence=Displayed;
CC       Name=2; Synonyms=flr_tv1;
CC         IsoId=A7YE96-2; Sequence=VSP_033487, VSP_033488, VSP_033489;
CC   -!- TISSUE SPECIFICITY: Localizes to the cilia of many ciliated epithelial
CC       cell types including pronephric cells, olfactory placode, the brain
CC       ventricle and lateral line organs. {ECO:0000269|PubMed:17761526}.
CC   -!- DEVELOPMENTAL STAGE: Maternally expressed. First detected in Kupffer's
CC       vesicle of seven somite embryos and the lateral mesoderm of 11 somite
CC       embryos. By 24 to 30 hours post-fertilization (hpf), expression becomes
CC       widespread within the central nervous system and the pronephros and
CC       parallels the development of multiciliated cells. During later stages
CC       of larval development (54 hpf), expression continues throughout the
CC       pronephros and intensifies in the olfactory placode and lateral line
CC       organs. {ECO:0000269|PubMed:17761526}.
CC   -!- SIMILARITY: Belongs to the TTC30/dfy-1/fleer family. {ECO:0000305}.
CC   -!- SEQUENCE CAUTION:
CC       Sequence=ABV08791.1; Type=Erroneous initiation; Evidence={ECO:0000305};
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DR   EMBL; EF653429; ABV08791.1; ALT_INIT; mRNA.
DR   EMBL; EU124003; ABV26099.1; -; mRNA.
DR   EMBL; BX510989; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR   EMBL; BC163912; AAI63912.1; -; mRNA.
DR   RefSeq; NP_001098119.2; NM_001104649.2. [A7YE96-1]
DR   AlphaFoldDB; A7YE96; -.
DR   SMR; A7YE96; -.
DR   STRING; 7955.ENSDARP00000055421; -.
DR   PaxDb; A7YE96; -.
DR   PeptideAtlas; A7YE96; -.
DR   Ensembl; ENSDART00000055422; ENSDARP00000055421; ENSDARG00000038024. [A7YE96-1]
DR   GeneID; 797829; -.
DR   KEGG; dre:797829; -.
DR   CTD; 797829; -.
DR   ZFIN; ZDB-GENE-070117-2066; flr.
DR   eggNOG; KOG4340; Eukaryota.
DR   GeneTree; ENSGT00390000010116; -.
DR   HOGENOM; CLU_023760_0_0_1; -.
DR   InParanoid; A7YE96; -.
DR   OMA; MRNHPEL; -.
DR   OrthoDB; 247838at2759; -.
DR   PhylomeDB; A7YE96; -.
DR   TreeFam; TF314592; -.
DR   Reactome; R-DRE-5620924; Intraflagellar transport.
DR   PRO; PR:A7YE96; -.
DR   Proteomes; UP000000437; Genome assembly.
DR   Proteomes; UP000814640; Chromosome 3.
DR   Bgee; ENSDARG00000038024; Expressed in testis and 29 other tissues.
DR   ExpressionAtlas; A7YE96; baseline.
DR   GO; GO:0005879; C:axonemal microtubule; IDA:UniProtKB.
DR   GO; GO:0005929; C:cilium; IDA:UniProtKB.
DR   GO; GO:0030992; C:intraciliary transport particle B; IBA:GO_Central.
DR   GO; GO:0031514; C:motile cilium; IDA:ZFIN.
DR   GO; GO:0120170; F:intraciliary transport particle B binding; IBA:GO_Central.
DR   GO; GO:0070738; F:tubulin-glycine ligase activity; IMP:ZFIN.
DR   GO; GO:0060271; P:cilium assembly; IMP:ZFIN.
DR   GO; GO:0007368; P:determination of left/right symmetry; IMP:ZFIN.
DR   GO; GO:0001736; P:establishment of planar polarity; IGI:ZFIN.
DR   GO; GO:0042073; P:intraciliary transport; IMP:UniProtKB.
DR   GO; GO:0036372; P:opsin transport; IGI:ZFIN.
DR   GO; GO:0018095; P:protein polyglutamylation; IMP:UniProtKB.
DR   Gene3D; 1.25.40.10; -; 3.
DR   InterPro; IPR011990; TPR-like_helical_dom_sf.
DR   InterPro; IPR013105; TPR_2.
DR   InterPro; IPR019734; TPR_repeat.
DR   InterPro; IPR039941; TT30.
DR   PANTHER; PTHR20931; PTHR20931; 1.
DR   Pfam; PF07719; TPR_2; 1.
DR   Pfam; PF13174; TPR_6; 1.
DR   SMART; SM00028; TPR; 3.
DR   SUPFAM; SSF48452; SSF48452; 2.
DR   PROSITE; PS50005; TPR; 4.
DR   PROSITE; PS50293; TPR_REGION; 3.
PE   2: Evidence at transcript level;
KW   Alternative splicing; Cell projection; Cilium;
KW   Cilium biogenesis/degradation; Coiled coil; Reference proteome; Repeat;
KW   TPR repeat.
FT   CHAIN           1..651
FT                   /note="Tetratricopeptide repeat protein 30A"
FT                   /id="PRO_0000333206"
FT   REPEAT          8..41
FT                   /note="TPR 1"
FT   REPEAT          42..75
FT                   /note="TPR 2"
FT   REPEAT          140..173
FT                   /note="TPR 3"
FT   REPEAT          175..207
FT                   /note="TPR 4"
FT   REPEAT          379..410
FT                   /note="TPR 5"
FT   REPEAT          411..443
FT                   /note="TPR 6"
FT   REPEAT          445..478
FT                   /note="TPR 7"
FT   REPEAT          530..563
FT                   /note="TPR 8"
FT   COILED          494..521
FT                   /evidence="ECO:0000255"
FT   VAR_SEQ         379..382
FT                   /note="Missing (in isoform 2)"
FT                   /evidence="ECO:0000303|PubMed:17761526"
FT                   /id="VSP_033487"
FT   VAR_SEQ         538..557
FT                   /note="TLYCAKGNYDFGISRVIKSL -> LEQTHGSTVFSRCWKTWPST (in
FT                   isoform 2)"
FT                   /evidence="ECO:0000303|PubMed:17761526"
FT                   /id="VSP_033488"
FT   VAR_SEQ         558..651
FT                   /note="Missing (in isoform 2)"
FT                   /evidence="ECO:0000303|PubMed:17761526"
FT                   /id="VSP_033489"
FT   CONFLICT        573
FT                   /note="K -> R (in Ref. 1; ABV08791)"
FT                   /evidence="ECO:0000305"
SQ   SEQUENCE   651 AA;  75523 MW;  8EB0F30CB970D65E CRC64;
     MPPMTIKDGE YTATVYKMIK EGRYGDAIHI LSKEHQKHTK SRAALSLLGY CYYHMQDFTN
     AAECYEQLTQ LHPEVEDYKL YYAQSLYGAC AFPEAMKSTF LLDNTTSHTK MIKLQAAIKY
     GEEDYSGAKT LVEQLPQEDP DYDVDLGCLL YKEGEFEEAC KKFMSSMNVL GYQPDLAYNI
     ALCYYSLKQY ASALKYIAEI IERGIREHPE LSIGMTTEGI DVRSVGNTLI LHETALIEAF
     NLKAAIEYQL KNYAAAQEAL TDMPPRSEEE LDPVTLHNQA LMNMDTKPTE GFEKLAFLLQ
     QNPFPPVTFG NLLLLYCKYE YFDLAADVLA ENAHLTYKFL TPYLYEFLDA MITCQTAPEE
     AFRKFDENAG KLTEQLRKVT KQVQEARHNR DDESLKKYVQ DYDEVLEKYI PVLMAQAKIY
     WNRENYSMVE KIFHKSLEFC NEHDTWKLNV AHVLFMQDNK YKEAIGFYEP IVKKHYENIL
     NVSAIVLANL CVSYIMTSQN EEAEELMRKI EKEEEQISYD DPDKKIFHLC IVNLVIGTLY
     CAKGNYDFGI SRVIKSLEPY NKKLGTDTWF YAKRCFLSLL ENMAKHMIML RDSVVQECIQ
     FLEHCELYGK DVLAIIEQPL EEDRMHIGKN TVTYESRLIK ALFYEVTGWN E