ID   TT30B_MOUSE             Reviewed;         664 AA.
AC   Q9CY00; Q3UI02;
DT   20-MAY-2008, integrated into UniProtKB/Swiss-Prot.
DT   01-JUN-2001, sequence version 1.
DT   03-AUG-2022, entry version 142.
DE   RecName: Full=Tetratricopeptide repeat protein 30B;
DE            Short=TPR repeat protein 30B;
GN   Name=Ttc30b;
OS   Mus musculus (Mouse).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC   Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC   Murinae; Mus; Mus.
OX   NCBI_TaxID=10090;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   STRAIN=C57BL/6J; TISSUE=Kidney, and Liver;
RX   PubMed=16141072; DOI=10.1126/science.1112014;
RA   Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N.,
RA   Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K.,
RA   Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.,
RA   Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R.,
RA   Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T.,
RA   Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A.,
RA   Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B.,
RA   Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M.,
RA   Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S.,
RA   Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E.,
RA   Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D.,
RA   Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M.,
RA   Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H.,
RA   Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V.,
RA   Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S.,
RA   Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H.,
RA   Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N.,
RA   Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F.,
RA   Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G.,
RA   Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z.,
RA   Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C.,
RA   Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y.,
RA   Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S.,
RA   Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K.,
RA   Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R.,
RA   van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H.,
RA   Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M.,
RA   Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C.,
RA   Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S.,
RA   Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K.,
RA   Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M.,
RA   Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C.,
RA   Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A.,
RA   Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.;
RT   "The transcriptional landscape of the mammalian genome.";
RL   Science 309:1559-1563(2005).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=C57BL/6J;
RX   PubMed=19468303; DOI=10.1371/journal.pbio.1000112;
RA   Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X.,
RA   Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y.,
RA   Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S.,
RA   Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R.,
RA   Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K.,
RA   Eichler E.E., Ponting C.P.;
RT   "Lineage-specific biology revealed by a finished genome assembly of the
RT   mouse.";
RL   PLoS Biol. 7:E1000112-E1000112(2009).
RN   [3]
RP   INTERACTION WITH IFT27; IFT46; IFT74; IFT52; IFT57; IFT80; IFT81; IFT88 AND
RP   KIF17.
RX   PubMed=23810713; DOI=10.1016/j.yexcr.2013.06.010;
RA   Howard P.W., Jue S.F., Maurer R.A.;
RT   "Interaction of mouse TTC30/DYF-1 with multiple intraflagellar transport
RT   complex B proteins and KIF17.";
RL   Exp. Cell Res. 319:2275-2281(2013).
CC   -!- FUNCTION: Required for polyglutamylation of axonemal tubulin. Plays a
CC       role in anterograde intraflagellar transport (IFT), the process by
CC       which cilia precursors are transported from the base of the cilium to
CC       the site of their incorporation at the tip. {ECO:0000250}.
CC   -!- SUBUNIT: Interacts with the IFT B complex components IFT27, IFT46,
CC       IFT74, IFT52, IFT57, IFT80, IFT81 and IFT88 (PubMed:23810713).
CC       Interacts with KIF17 (PubMed:23810713). {ECO:0000269|PubMed:23810713}.
CC   -!- SUBCELLULAR LOCATION: Cell projection, cilium {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the TTC30/dfy-1/fleer family. {ECO:0000305}.
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DR   EMBL; AK011097; BAB27394.1; -; mRNA.
DR   EMBL; AK147134; BAE27704.1; -; mRNA.
DR   EMBL; AL773533; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR   CCDS; CCDS38150.1; -.
DR   RefSeq; NP_082511.1; NM_028235.1.
DR   AlphaFoldDB; Q9CY00; -.
DR   SMR; Q9CY00; -.
DR   BioGRID; 215366; 1.
DR   ComplexPortal; CPX-5028; IFT-B complex.
DR   IntAct; Q9CY00; 1.
DR   MINT; Q9CY00; -.
DR   STRING; 10090.ENSMUSP00000097576; -.
DR   iPTMnet; Q9CY00; -.
DR   PhosphoSitePlus; Q9CY00; -.
DR   jPOST; Q9CY00; -.
DR   MaxQB; Q9CY00; -.
DR   PaxDb; Q9CY00; -.
DR   PRIDE; Q9CY00; -.
DR   ProteomicsDB; 300146; -.
DR   Ensembl; ENSMUST00000099996; ENSMUSP00000097576; ENSMUSG00000075273.
DR   GeneID; 72421; -.
DR   KEGG; mmu:72421; -.
DR   UCSC; uc008keu.1; mouse.
DR   CTD; 150737; -.
DR   MGI; MGI:1919671; Ttc30b.
DR   VEuPathDB; HostDB:ENSMUSG00000075273; -.
DR   eggNOG; KOG4340; Eukaryota.
DR   GeneTree; ENSGT00390000010116; -.
DR   HOGENOM; CLU_023760_0_0_1; -.
DR   InParanoid; Q9CY00; -.
DR   OMA; HMILIRD; -.
DR   OrthoDB; 247838at2759; -.
DR   PhylomeDB; Q9CY00; -.
DR   TreeFam; TF314592; -.
DR   Reactome; R-MMU-5620924; Intraflagellar transport.
DR   BioGRID-ORCS; 72421; 3 hits in 72 CRISPR screens.
DR   ChiTaRS; Ttc30b; mouse.
DR   PRO; PR:Q9CY00; -.
DR   Proteomes; UP000000589; Chromosome 2.
DR   RNAct; Q9CY00; protein.
DR   Bgee; ENSMUSG00000075273; Expressed in olfactory epithelium and 250 other tissues.
DR   ExpressionAtlas; Q9CY00; baseline and differential.
DR   Genevisible; Q9CY00; MM.
DR   GO; GO:0005879; C:axonemal microtubule; IBA:GO_Central.
DR   GO; GO:0036064; C:ciliary basal body; ISO:MGI.
DR   GO; GO:0005929; C:cilium; IC:ComplexPortal.
DR   GO; GO:0030992; C:intraciliary transport particle B; IDA:MGI.
DR   GO; GO:0120170; F:intraciliary transport particle B binding; IBA:GO_Central.
DR   GO; GO:0060271; P:cilium assembly; IC:ComplexPortal.
DR   GO; GO:0035720; P:intraciliary anterograde transport; IC:ComplexPortal.
DR   GO; GO:0042073; P:intraciliary transport; IBA:GO_Central.
DR   Gene3D; 1.25.40.10; -; 3.
DR   InterPro; IPR011990; TPR-like_helical_dom_sf.
DR   InterPro; IPR019734; TPR_repeat.
DR   InterPro; IPR039941; TT30.
DR   PANTHER; PTHR20931; PTHR20931; 1.
DR   Pfam; PF13176; TPR_7; 1.
DR   SMART; SM00028; TPR; 4.
DR   SUPFAM; SSF48452; SSF48452; 3.
DR   PROSITE; PS50293; TPR_REGION; 2.
PE   1: Evidence at protein level;
KW   Cell projection; Cilium; Cilium biogenesis/degradation; Coiled coil;
KW   Reference proteome; Repeat; TPR repeat.
FT   CHAIN           1..664
FT                   /note="Tetratricopeptide repeat protein 30B"
FT                   /id="PRO_0000333204"
FT   REPEAT          11..44
FT                   /note="TPR 1"
FT   REPEAT          45..78
FT                   /note="TPR 2"
FT   REPEAT          153..186
FT                   /note="TPR 3"
FT   REPEAT          188..220
FT                   /note="TPR 4"
FT   REPEAT          385..418
FT                   /note="TPR 5"
FT   REPEAT          423..456
FT                   /note="TPR 6"
FT   REPEAT          458..491
FT                   /note="TPR 7"
FT   REPEAT          543..576
FT                   /note="TPR 8"
FT   COILED          507..534
FT                   /evidence="ECO:0000255"
FT   CONFLICT        493
FT                   /note="L -> P (in Ref. 1; BAE27704)"
FT                   /evidence="ECO:0000305"
SQ   SEQUENCE   664 AA;  76096 MW;  8BCB2D1F1A7099F3 CRC64;
     MAGLSSSQIP DGEFTAVVYR LIRDSRYSEA VQLLSAELQR SSRSRAGLSL LAYCYYRLQE
     FELAAECYEQ LSQMHPELEQ YRLYQAQALY KACLYPEATR VAFLLDNPAY QTRVLRLQAA
     IKYSEGDLPG ARSLVEQLLS GEGVEDSGGE SDYDGQINLG CLLYKEGHYE AACSKFLAAL
     QASGYQPDLS YNLALAYYSS RQYAPALKHI ADIIERGIRQ HPELGVGMTT EGIDVRSVGN
     TIVLHQTALV EAFNLKAAIE YQLRNYEAAQ EALTDMPPRA EEELDPVTLH NQALMNMDAK
     PTEGFEKLQF LLQQNPFPPE TFGNLLLLYC KYEYFDLAAD VLAENAHLTY KFLTPYLYDF
     LDAMITCQTA PEEAFIKLDG LAGMLTEQLR KLTIQVQDSR HSRDDESAKK AVNEYDETLE
     KYIPVLMAQA KIYWNFENYP MVEKIFRKSV EFCNDHDVWK LNVAHVLFMQ ENKYKEAIGF
     YEPIVKKNYD NILSVSAIVL ANLCVSYIMT SQNEEAEELM RKIEKEEEQL SYGDPDKKIY
     HLCIVNLVIG TLYCAKGNYD FGISRVIKSL EPYHKKLGTD TWYYAKRCFL SLLENMSKHT
     IMLRDSVIQE CVQFLEHCEL YGRNIPAVIE QPLEEERMHT GKNTVTYESR KLRALIYEII
     GWNV