ID   TT9_ARATH               Reviewed;         837 AA.
AC   Q8W4P9; C0Z3C5; Q9LSJ0;
DT   28-FEB-2018, integrated into UniProtKB/Swiss-Prot.
DT   01-MAR-2002, sequence version 1.
DT   03-AUG-2022, entry version 116.
DE   RecName: Full=Protein TRANSPARENT TESTA 9 {ECO:0000303|PubMed:8528278};
DE   AltName: Full=Protein GREEN FLUORESCENT SEED 9 {ECO:0000303|PubMed:25116949};
GN   Name=TT9 {ECO:0000303|PubMed:8528278};
GN   Synonyms=GFS9 {ECO:0000303|PubMed:25116949};
GN   OrderedLocusNames=At3g28430 {ECO:0000312|Araport:AT3G28430};
GN   ORFNames=MFJ20.13 {ECO:0000312|EMBL:BAB02860.1};
OS   Arabidopsis thaliana (Mouse-ear cress).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC   rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX   NCBI_TaxID=3702;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=cv. Columbia;
RX   PubMed=10819329; DOI=10.1093/dnares/7.2.131;
RA   Sato S., Nakamura Y., Kaneko T., Katoh T., Asamizu E., Tabata S.;
RT   "Structural analysis of Arabidopsis thaliana chromosome 3. I. Sequence
RT   features of the regions of 4,504,864 bp covered by sixty P1 and TAC
RT   clones.";
RL   DNA Res. 7:131-135(2000).
RN   [2]
RP   GENOME REANNOTATION.
RC   STRAIN=cv. Columbia;
RX   PubMed=27862469; DOI=10.1111/tpj.13415;
RA   Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA   Town C.D.;
RT   "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT   genome.";
RL   Plant J. 89:789-804(2017).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   STRAIN=cv. Columbia;
RX   PubMed=14593172; DOI=10.1126/science.1088305;
RA   Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M.,
RA   Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G.,
RA   Liu S.X., Lam B., Sakano H., Wu T., Yu G., Miranda M., Quach H.L.,
RA   Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C.,
RA   Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J.,
RA   Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A.,
RA   Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C.,
RA   Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X.,
RA   Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M.,
RA   Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K.,
RA   Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A.,
RA   Ecker J.R.;
RT   "Empirical analysis of transcriptional activity in the Arabidopsis
RT   genome.";
RL   Science 302:842-846(2003).
RN   [4]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 441-837.
RC   STRAIN=cv. Columbia;
RX   PubMed=19423640; DOI=10.1093/dnares/dsp009;
RA   Iida K., Fukami-Kobayashi K., Toyoda A., Sakaki Y., Kobayashi M., Seki M.,
RA   Shinozaki K.;
RT   "Analysis of multiple occurrences of alternative splicing events in
RT   Arabidopsis thaliana using novel sequenced full-length cDNAs.";
RL   DNA Res. 16:155-164(2009).
RN   [5]
RP   DISRUPTION PHENOTYPE.
RX   PubMed=8528278; DOI=10.1046/j.1365-313x.1995.08050659.x;
RA   Shirley B.W., Kubasek W.L., Storz G., Bruggemann E., Koornneef M.,
RA   Ausubel F.M., Goodman H.M.;
RT   "Analysis of Arabidopsis mutants deficient in flavonoid biosynthesis.";
RL   Plant J. 8:659-671(1995).
RN   [6]
RP   FUNCTION, SUBCELLULAR LOCATION, AND DISRUPTION PHENOTYPE.
RX   PubMed=25116949; DOI=10.1111/tpj.12637;
RA   Ichino T., Fuji K., Ueda H., Takahashi H., Koumoto Y., Takagi J.,
RA   Tamura K., Sasaki R., Aoki K., Shimada T., Hara-Nishimura I.;
RT   "GFS9/TT9 contributes to intracellular membrane trafficking and flavonoid
RT   accumulation in Arabidopsis thaliana.";
RL   Plant J. 80:410-423(2014).
CC   -!- FUNCTION: Involved in membrane trafficking and vacuole development
CC       through membrane fusion at the vacuole. Required for membrane
CC       trafficking machinery and accumulation of flavonoids in the seed coat.
CC       {ECO:0000269|PubMed:25116949}.
CC   -!- SUBCELLULAR LOCATION: Golgi apparatus membrane
CC       {ECO:0000269|PubMed:25116949}; Peripheral membrane protein
CC       {ECO:0000269|PubMed:25116949}.
CC   -!- DISRUPTION PHENOTYPE: Abnormal pale brown color of seed coat (testa)
CC       due to low flavonoid accumulation levels. {ECO:0000269|PubMed:25116949,
CC       ECO:0000269|PubMed:8528278}.
CC   -!- SIMILARITY: Belongs to the CLEC16A/gop-1 family. {ECO:0000305}.
CC   -!- SEQUENCE CAUTION:
CC       Sequence=BAB02860.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
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DR   EMBL; AB026644; BAB02860.1; ALT_SEQ; Genomic_DNA.
DR   EMBL; CP002686; AEE77444.1; -; Genomic_DNA.
DR   EMBL; AY062117; AAL32989.1; -; mRNA.
DR   EMBL; AK319089; BAH57204.1; -; mRNA.
DR   RefSeq; NP_566837.1; NM_113763.3.
DR   AlphaFoldDB; Q8W4P9; -.
DR   SMR; Q8W4P9; -.
DR   IntAct; Q8W4P9; 1.
DR   STRING; 3702.AT3G28430.1; -.
DR   iPTMnet; Q8W4P9; -.
DR   PaxDb; Q8W4P9; -.
DR   PRIDE; Q8W4P9; -.
DR   ProteomicsDB; 232357; -.
DR   EnsemblPlants; AT3G28430.1; AT3G28430.1; AT3G28430.
DR   GeneID; 822473; -.
DR   Gramene; AT3G28430.1; AT3G28430.1; AT3G28430.
DR   KEGG; ath:AT3G28430; -.
DR   Araport; AT3G28430; -.
DR   TAIR; locus:2088917; AT3G28430.
DR   eggNOG; KOG2219; Eukaryota.
DR   HOGENOM; CLU_017600_0_0_1; -.
DR   InParanoid; Q8W4P9; -.
DR   OMA; DEKHAKW; -.
DR   OrthoDB; 1319544at2759; -.
DR   PhylomeDB; Q8W4P9; -.
DR   PRO; PR:Q8W4P9; -.
DR   Proteomes; UP000006548; Chromosome 3.
DR   ExpressionAtlas; Q8W4P9; baseline and differential.
DR   GO; GO:0036020; C:endolysosome membrane; IBA:GO_Central.
DR   GO; GO:0019898; C:extrinsic component of membrane; IDA:TAIR.
DR   GO; GO:0005794; C:Golgi apparatus; IDA:TAIR.
DR   GO; GO:0000139; C:Golgi membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0016021; C:integral component of membrane; IBA:GO_Central.
DR   GO; GO:0009718; P:anthocyanin-containing compound biosynthetic process; IDA:TAIR.
DR   GO; GO:0001708; P:cell fate specification; IMP:TAIR.
DR   GO; GO:0016197; P:endosomal transport; IBA:GO_Central.
DR   GO; GO:0008333; P:endosome to lysosome transport; IBA:GO_Central.
DR   GO; GO:1903415; P:flavonoid transport from endoplasmic reticulum to plant-type vacuole; IMP:TAIR.
DR   GO; GO:0044090; P:positive regulation of vacuole organization; IMP:TAIR.
DR   GO; GO:1901096; P:regulation of autophagosome maturation; IBA:GO_Central.
DR   GO; GO:0016192; P:vesicle-mediated transport; IMP:TAIR.
DR   InterPro; IPR016024; ARM-type_fold.
DR   InterPro; IPR039272; CLEC16A/TT9.
DR   InterPro; IPR019155; CLEC16A/TT9_N.
DR   PANTHER; PTHR21481; PTHR21481; 1.
DR   Pfam; PF09758; FPL; 1.
DR   SUPFAM; SSF48371; SSF48371; 1.
PE   2: Evidence at transcript level;
KW   Golgi apparatus; Membrane; Reference proteome.
FT   CHAIN           1..837
FT                   /note="Protein TRANSPARENT TESTA 9"
FT                   /id="PRO_0000443285"
FT   DOMAIN          42..192
FT                   /note="FPL"
FT                   /evidence="ECO:0000255"
FT   REGION          366..386
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   CONFLICT        773
FT                   /note="T -> A (in Ref. 4; BAH57204)"
FT                   /evidence="ECO:0000305"
SQ   SEQUENCE   837 AA;  94742 MW;  5B6F3C0AFABCFF65 CRC64;
     MWLSFLRPRD RFSLAELRYL TDQLRKIQIV NEANKDLVIE ALRSIAEILT YGDQHDPLFF
     EFFMEKQVMG EFVRILRVSK TVTVSVQLLQ TMSIMIQNLK SEQAIYYLFS NEYVNYLITY
     TFDFQHEELL SYYISFLRAV SGKLNKHTIS LLLKTENDVV VSFPLYVEGI QFAFHEENMI
     RTAVRALTLN VYHVGDESVN DYVVSPPHTE YFSKLISFFQ KQCMDLSAMV LNTLKSPSPD
     SGGKLFSAVD GIEDTLYYFS DVISAGIPDI GRLITDHILQ HLTLPLLLPS LCSEAVNDIS
     VDPVTSLYLL SCILRIVKIK DLANMTAATL FCPVKAFISS SLVKPNSSLA PEGLTYVNGH
     PDKGVTEEAN QQCSSTAAGM SDDGNSHLCS EDTPKSIFNN SHMTFRETLL QYISEGDDVQ
     AQGSLFVLAT LLQTKELEES MLDAFGILPQ RKQHKKLLLQ SLVGEDTGEE QLFSPRNGSM
     RDGLSSELDW YLRRLEEQFG VCCSLPGAAR CPRVHRHQVV DTLVTLLCRE NISAETLWDG
     GWLLRQLLPY SEAEFNRKHL KMLNVSYEKC KNSLTREIKG IWPDLLIRVL LDEWRKCKRV
     IEAPSPQKEP KSVLLQLDRS SSNDNSVSES SFTAGERMCE VVKVFVLLHQ LQIFSLGRSL
     PEQPPIYPPA DRSETSRATR AGLDVSVPKP GTELKLVDAV PCRIAFERGK ERDFSFLALS
     SGESGWIVLA DPDNGIVRVT APLAGCKPRI DEKHPRWLHL RIRPSTLPLL DPTKRGVYEK
     LKSKGLVDGR WILAFRDDES CHSAYSMVAG EIDLQCSEVE RRLRPLFDLE RNQLEDQ