C4AA1_DROME
ID C4AA1_DROME Reviewed; 510 AA.
AC Q9V7G5; Q1RKQ6;
DT 01-DEC-2000, integrated into UniProtKB/Swiss-Prot.
DT 14-APR-2009, sequence version 2.
DT 03-AUG-2022, entry version 151.
DE RecName: Full=Probable cytochrome P450 4aa1;
DE EC=1.14.-.-;
DE AltName: Full=CYPIVAA1;
GN Name=Cyp4aa1; ORFNames=CG8302;
OS Drosophila melanogaster (Fruit fly).
OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea;
OC Drosophilidae; Drosophila; Sophophora.
OX NCBI_TaxID=7227;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Berkeley;
RX PubMed=10731132; DOI=10.1126/science.287.5461.2185;
RA Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D.,
RA Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F.,
RA George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N.,
RA Sutton G.G., Wortman J.R., Yandell M.D., Zhang Q., Chen L.X., Brandon R.C.,
RA Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C.,
RA Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A.,
RA An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A.,
RA Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V.,
RA Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J.,
RA Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E.,
RA Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B.,
RA Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I.,
RA Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C.,
RA Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S.,
RA Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M.,
RA Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M.,
RA Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D.,
RA Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F.,
RA Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D.,
RA Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A.,
RA Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C.,
RA McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C.,
RA Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L.,
RA Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R.,
RA Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V.,
RA Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F.,
RA Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J.,
RA Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R.,
RA Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y.,
RA Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T.,
RA Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S.,
RA Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W.,
RA Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M.,
RA Venter J.C.;
RT "The genome sequence of Drosophila melanogaster.";
RL Science 287:2185-2195(2000).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=Berkeley;
RX PubMed=12537572; DOI=10.1186/gb-2002-3-12-research0083;
RA Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S.,
RA Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E.,
RA Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P.,
RA Bettencourt B.R., Celniker S.E., de Grey A.D.N.J., Drysdale R.A.,
RA Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M.,
RA Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E.;
RT "Annotation of the Drosophila melanogaster euchromatic genome: a systematic
RT review.";
RL Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM A).
RC STRAIN=Berkeley;
RA Stapleton M., Carlson J.W., Chavez C., Frise E., George R.A., Pacleb J.M.,
RA Park S., Wan K.H., Yu C., Celniker S.E.;
RL Submitted (APR-2006) to the EMBL/GenBank/DDBJ databases.
CC -!- FUNCTION: May be involved in the metabolism of insect hormones and in
CC the breakdown of synthetic insecticides. {ECO:0000250}.
CC -!- COFACTOR:
CC Name=heme; Xref=ChEBI:CHEBI:30413; Evidence={ECO:0000250};
CC -!- SUBCELLULAR LOCATION: Endoplasmic reticulum membrane {ECO:0000305};
CC Peripheral membrane protein {ECO:0000305}. Microsome membrane
CC {ECO:0000305}; Peripheral membrane protein {ECO:0000305}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=B;
CC IsoId=Q9V7G5-1; Sequence=Displayed;
CC Name=A;
CC IsoId=Q9V7G5-2; Sequence=VSP_036909, VSP_036910;
CC -!- SIMILARITY: Belongs to the cytochrome P450 family. {ECO:0000305}.
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DR EMBL; AE013599; AAF58091.2; -; Genomic_DNA.
DR EMBL; BT025154; ABE73324.1; -; mRNA.
DR RefSeq; NP_611067.2; NM_137223.3. [Q9V7G5-1]
DR AlphaFoldDB; Q9V7G5; -.
DR SMR; Q9V7G5; -.
DR STRING; 7227.FBpp0113050; -.
DR PaxDb; Q9V7G5; -.
DR PRIDE; Q9V7G5; -.
DR EnsemblMetazoa; FBtr0114558; FBpp0113050; FBgn0034053. [Q9V7G5-1]
DR GeneID; 36752; -.
DR KEGG; dme:Dmel_CG8302; -.
DR UCSC; CG8302-RB; d. melanogaster. [Q9V7G5-1]
DR CTD; 36752; -.
DR FlyBase; FBgn0034053; Cyp4aa1.
DR VEuPathDB; VectorBase:FBgn0034053; -.
DR eggNOG; KOG0157; Eukaryota.
DR GeneTree; ENSGT00940000166362; -.
DR HOGENOM; CLU_001570_5_1_1; -.
DR InParanoid; Q9V7G5; -.
DR OMA; VNNCVLD; -.
DR PhylomeDB; Q9V7G5; -.
DR Reactome; R-DME-193144; Estrogen biosynthesis.
DR Reactome; R-DME-211976; Endogenous sterols.
DR BioGRID-ORCS; 36752; 0 hits in 1 CRISPR screen.
DR GenomeRNAi; 36752; -.
DR PRO; PR:Q9V7G5; -.
DR Proteomes; UP000000803; Chromosome 2R.
DR Bgee; FBgn0034053; Expressed in proventriculus outer layer (Drosophila) and 14 other tissues.
DR Genevisible; Q9V7G5; DM.
DR GO; GO:0005789; C:endoplasmic reticulum membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0020037; F:heme binding; IEA:InterPro.
DR GO; GO:0005506; F:iron ion binding; IEA:InterPro.
DR GO; GO:0004497; F:monooxygenase activity; IEA:UniProtKB-KW.
DR GO; GO:0016705; F:oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen; IEA:InterPro.
DR GO; GO:0006697; P:ecdysone biosynthetic process; IBA:GO_Central.
DR Gene3D; 1.10.630.10; -; 1.
DR InterPro; IPR001128; Cyt_P450.
DR InterPro; IPR017972; Cyt_P450_CS.
DR InterPro; IPR002403; Cyt_P450_E_grp-IV.
DR InterPro; IPR036396; Cyt_P450_sf.
DR Pfam; PF00067; p450; 1.
DR PRINTS; PR00465; EP450IV.
DR PRINTS; PR00385; P450.
DR SUPFAM; SSF48264; SSF48264; 1.
DR PROSITE; PS00086; CYTOCHROME_P450; 1.
PE 2: Evidence at transcript level;
KW Alternative splicing; Endoplasmic reticulum; Heme; Iron; Membrane;
KW Metal-binding; Microsome; Monooxygenase; Oxidoreductase;
KW Reference proteome.
FT CHAIN 1..510
FT /note="Probable cytochrome P450 4aa1"
FT /id="PRO_0000051825"
FT BINDING 450
FT /ligand="heme"
FT /ligand_id="ChEBI:CHEBI:30413"
FT /ligand_part="Fe"
FT /ligand_part_id="ChEBI:CHEBI:18248"
FT /note="axial binding residue"
FT /evidence="ECO:0000250"
FT VAR_SEQ 348..375
FT /note="DSNRAPTMTDLHEMRYMEMCIKEALRLY -> FLWGIYIYFLLKQCYICFIC
FT NKVMLVGI (in isoform A)"
FT /evidence="ECO:0000303|Ref.3"
FT /id="VSP_036909"
FT VAR_SEQ 376..510
FT /note="Missing (in isoform A)"
FT /evidence="ECO:0000303|Ref.3"
FT /id="VSP_036910"
SQ SEQUENCE 510 AA; 58536 MW; 91FD50E2E325D3DF CRC64;
MFVEKVLERT TNLELCSILI LLVISLSIYT FYATLNTYLR SVLLSLRLTG PPSLPFLGNC
MLVTDKDLMR RCAGKAFDLY GSLVRIWVLL FPFFAVLEPE DLQVILSSKK HTNKVFFYRL
MHNFLGDGLI TSSGSKWSNH RRLIQPAFHH NLLEKFIDTF VDASQSLYEN LDAEAVGTEI
NIAKYVNNCV LDILNEAVLG VPIKKRGQDV AMMEDSPFRQ GKIMMPARFT QPWLLLDGIY
HWTKMANDEL NQKKRLNDFT RKMIQRRRQI QNNNNGNSER KCLLDHMIEI SESNRDFTEE
DIVNEACTFM LAGQDSVGAA VAFTLFLLTQ NPECQDRCVL ELATIFEDSN RAPTMTDLHE
MRYMEMCIKE ALRLYPSVPL IARKLGEEVR LAKHTLPAGS NVFICPYATH RLAHIYPDPE
KFQPERFSPE NSENRHPYAF LPFSAGPRYC IGNRFAIMEI KTIVSRLLRS YQLLPVTGKT
TIAATFRITL RASGGLWVRL KERDHPLIAH
