TTC21_CAEEL
ID TTC21_CAEEL Reviewed; 1324 AA.
AC Q20255; Q23468; Q7Z146;
DT 01-NOV-1997, integrated into UniProtKB/Swiss-Prot.
DT 19-OCT-2011, sequence version 3.
DT 03-AUG-2022, entry version 151.
DE RecName: Full=Tetratricopeptide repeat protein 21 homolog;
DE Short=TPR repeat protein 21 homolog;
DE AltName: Full=Intraflagellar transport protein 139 homolog {ECO:0000303|PubMed:27515926};
GN Name=ift-139 {ECO:0000303|PubMed:27515926, ECO:0000312|WormBase:ZK328.7a};
GN ORFNames=ZK328.7 {ECO:0000312|WormBase:ZK328.7a};
OS Caenorhabditis elegans.
OC Eukaryota; Metazoa; Ecdysozoa; Nematoda; Chromadorea; Rhabditida;
OC Rhabditina; Rhabditomorpha; Rhabditoidea; Rhabditidae; Peloderinae;
OC Caenorhabditis.
OX NCBI_TaxID=6239;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA], AND ALTERNATIVE SPLICING.
RC STRAIN=Bristol N2;
RX PubMed=9851916; DOI=10.1126/science.282.5396.2012;
RG The C. elegans sequencing consortium;
RT "Genome sequence of the nematode C. elegans: a platform for investigating
RT biology.";
RL Science 282:2012-2018(1998).
RN [2]
RP FUNCTION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY, DISRUPTION PHENOTYPE,
RP AND MUTAGENESIS OF LEU-810.
RX PubMed=27515926; DOI=10.1038/srep31544;
RA Niwa S.;
RT "The nephronophthisis-related gene ift-139 is required for ciliogenesis in
RT Caenorhabditis elegans.";
RL Sci. Rep. 6:31544-31544(2016).
RN [3]
RP FUNCTION, IDENTIFICATION IN IFT COMPLEX A, SUBCELLULAR LOCATION, TISSUE
RP SPECIFICITY, DISRUPTION PHENOTYPE, AND IDENTIFICATION BY MASS SPECTROMETRY.
RX PubMed=28479320; DOI=10.1016/j.cub.2017.04.015;
RA Yi P., Li W.J., Dong M.Q., Ou G.;
RT "Dynein-driven retrograde intraflagellar transport is triphasic in C.
RT elegans sensory cilia.";
RL Curr. Biol. 27:1448-1461(2017).
CC -!- FUNCTION: Component of the IFT complex A (IFT-A), a complex required
CC for retrograde ciliary transport (PubMed:27515926, PubMed:28479320). In
CC particular, may act redundantly with the intraflagellar transport
CC protein ift-43 to regulate the transport of specific ciliary cargo
CC proteins such as che-3 which are related to motility (PubMed:28479320).
CC Functions in cilia biogenesis (PubMed:27515926).
CC {ECO:0000269|PubMed:27515926, ECO:0000269|PubMed:28479320}.
CC -!- SUBUNIT: Component of the IFT complex A (IFT-A) composed of at least
CC che-11, daf-10, dyf-2, ift-139, ift-43 and ifta-1.
CC {ECO:0000269|PubMed:28479320}.
CC -!- SUBCELLULAR LOCATION: Cell projection, cilium
CC {ECO:0000269|PubMed:27515926, ECO:0000269|PubMed:28479320}. Cytoplasm,
CC cytoskeleton, cilium basal body {ECO:0000269|PubMed:27515926}. Cell
CC projection, dendrite {ECO:0000269|PubMed:27515926}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=a {ECO:0000312|WormBase:ZK328.7a};
CC IsoId=Q20255-1; Sequence=Displayed;
CC Name=b {ECO:0000312|WormBase:ZK328.7b};
CC IsoId=Q20255-2; Sequence=VSP_041855;
CC -!- TISSUE SPECIFICITY: Expressed in ciliated sensory neurons in the head
CC and tail. {ECO:0000269|PubMed:27515926, ECO:0000269|PubMed:28479320}.
CC -!- DISRUPTION PHENOTYPE: Shorter cilia with an irregular morphology
CC resulting in functional defects characterized by defective movement,
CC dauer formation, and mating behavior (PubMed:27515926). Double knockout
CC with intraflagellar transport protein ift-43 results in defective
CC cilium morphology and function (PubMed:28479320).
CC {ECO:0000269|PubMed:27515926, ECO:0000269|PubMed:28479320}.
CC -!- SIMILARITY: Belongs to the TTC21 family. {ECO:0000305}.
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DR EMBL; FO081350; CCD70952.1; -; Genomic_DNA.
DR EMBL; FO081350; CCD70957.1; -; Genomic_DNA.
DR PIR; C88473; C88473.
DR PIR; T29012; T29012.
DR RefSeq; NP_001022993.2; NM_001027822.2. [Q20255-1]
DR RefSeq; NP_001022994.1; NM_001027823.2. [Q20255-2]
DR AlphaFoldDB; Q20255; -.
DR BioGRID; 41076; 2.
DR ComplexPortal; CPX-1289; Intraflagellar transport complex A.
DR IntAct; Q20255; 1.
DR STRING; 6239.ZK328.7a; -.
DR EPD; Q20255; -.
DR PaxDb; Q20255; -.
DR EnsemblMetazoa; ZK328.7a.1; ZK328.7a.1; WBGene00022696. [Q20255-1]
DR EnsemblMetazoa; ZK328.7b.1; ZK328.7b.1; WBGene00022696. [Q20255-2]
DR GeneID; 175855; -.
DR KEGG; cel:CELE_ZK328.7; -.
DR UCSC; ZK328.7a; c. elegans.
DR CTD; 175855; -.
DR WormBase; ZK328.7a; CE44936; WBGene00022696; ift-139. [Q20255-1]
DR WormBase; ZK328.7b; CE34449; WBGene00022696; ift-139. [Q20255-2]
DR eggNOG; ENOG502QQAB; Eukaryota.
DR GeneTree; ENSGT00390000005979; -.
DR HOGENOM; CLU_006149_0_0_1; -.
DR InParanoid; Q20255; -.
DR OMA; PEWGQQA; -.
DR OrthoDB; 45918at2759; -.
DR PhylomeDB; Q20255; -.
DR Reactome; R-CEL-5610787; Hedgehog 'off' state.
DR Reactome; R-CEL-5620924; Intraflagellar transport.
DR PRO; PR:Q20255; -.
DR Proteomes; UP000001940; Chromosome III.
DR Bgee; WBGene00022696; Expressed in pharyngeal muscle cell (C elegans) and 3 other tissues.
DR GO; GO:0005929; C:cilium; IC:ComplexPortal.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-KW.
DR GO; GO:0005856; C:cytoskeleton; IEA:UniProtKB-KW.
DR GO; GO:0030425; C:dendrite; IEA:UniProtKB-SubCell.
DR GO; GO:0030991; C:intraciliary transport particle A; IBA:GO_Central.
DR GO; GO:0035721; P:intraciliary retrograde transport; IBA:GO_Central.
DR GO; GO:0061512; P:protein localization to cilium; IBA:GO_Central.
DR Gene3D; 1.25.40.10; -; 4.
DR InterPro; IPR011990; TPR-like_helical_dom_sf.
DR InterPro; IPR019734; TPR_repeat.
DR InterPro; IPR040364; TTC21A/TTC21B.
DR PANTHER; PTHR14699; PTHR14699; 1.
DR Pfam; PF13176; TPR_7; 1.
DR SMART; SM00028; TPR; 9.
DR SUPFAM; SSF48452; SSF48452; 4.
DR PROSITE; PS50005; TPR; 11.
DR PROSITE; PS50293; TPR_REGION; 2.
PE 1: Evidence at protein level;
KW Alternative splicing; Cell projection; Cilium biogenesis/degradation;
KW Cytoplasm; Cytoskeleton; Reference proteome; Repeat; TPR repeat.
FT CHAIN 1..1324
FT /note="Tetratricopeptide repeat protein 21 homolog"
FT /id="PRO_0000106425"
FT REPEAT 58..91
FT /note="TPR 1"
FT REPEAT 414..446
FT /note="TPR 2"
FT REPEAT 582..615
FT /note="TPR 3"
FT REPEAT 669..702
FT /note="TPR 4"
FT REPEAT 737..770
FT /note="TPR 5"
FT REPEAT 772..804
FT /note="TPR 6"
FT REPEAT 806..837
FT /note="TPR 7"
FT REPEAT 847..880
FT /note="TPR 8"
FT REPEAT 894..927
FT /note="TPR 9"
FT REPEAT 929..961
FT /note="TPR 10"
FT REPEAT 963..995
FT /note="TPR 11"
FT REPEAT 997..1029
FT /note="TPR 12"
FT REPEAT 1033..1066
FT /note="TPR 13"
FT REPEAT 1205..1238
FT /note="TPR 14"
FT REPEAT 1240..1272
FT /note="TPR 15"
FT REPEAT 1274..1307
FT /note="TPR 16"
FT VAR_SEQ 1099..1324
FT /note="Missing (in isoform b)"
FT /evidence="ECO:0000305"
FT /id="VSP_041855"
FT MUTAGEN 810
FT /note="L->P: Defective cilia morphology."
FT /evidence="ECO:0000269|PubMed:27515926"
SQ SEQUENCE 1324 AA; 151169 MW; 680B8A0AFA49BD1F CRC64;
MDSESDDNPN DPEDRKKWGH KDVEHWRAVS NVHYYAREGY FGTAILVCDG RLATIKDPAL
AILKGVCLTL LGKIPDAIRH LETFVTDNDV ALGALHALKW AHASAFNPDN KSIVEIETEI
STRARNEKTP YTSYATASEV LYFAGEYQKS KQMLDIARKR ATEKHAKHYC LLGWIELALG
KKQKSTQELF EKAGGQEYPD GNIGRCKILE GHHSAPEMKV AANELAISTI HFLPGHIEKA
KASIMMKDWR GVMDCIMNAD QPEGSNPYIE VLRTVHGICY AGEVSMLKRT LQLLLKSLDE
NEATNHVLYA RITKLLVSIS GRDEKILRHA RDFLTRALKI SRKPDYVALS MRIAFGLGGA
KEVSTLSQEL VALDCEDSYA VLSSVVSMLM ISRVSDARAQ FDILPSAHPK LLESPLYYLI
ASVLAKQSKD KSFENFRQHI ENLVEMLRNQ LQSFPFGLDY LSLFSSDLLY SAVEQCFDFY
PLVPIKAPDD CMKLTAKTLQ MIYDVAPGLA HCTLQLARNS YLCSNTNAAE KWIEKVLDKD
DSLADAHILR AELILDRGGK ITDADDALVT GLNFNFKLRE TSLYHLIKSK TFKKRNENDE
AIKTLKMALQ IPRKEPSKNL FQPKESADTH KISVQLELID TLQHMKRIQE AETTMTDALA
EWAGQPEQDQ LVIAQAQLYL TKGHVERALG ILKKIQPGQS NFHLSRIKMA EIYLEEKKDK
RMFAACYREL LKVEATPGSY SLLGDAFMKV QEPEDAINFY EQALKMQSKD VQLAEKIGEA
YVMAHLYSKA VNFYESSMNI YKDKNMRLKL ANLLLKLRNF EKCEKVLRAP FERDPEPVGT
ETIQTYIQFL LLLAECHEMM DNVPEAMNDF EKAKSLHSRI QDKTLTAALK KEGARICNLQ
AELLYRRREF SQAVDICKQA LAYHETDLKA NLLLSKIFKE ENKWTLVLQP CQTVIQVDPH
NDEANSILAD FYYIRSEAAH ASTSYTTLLN TNPQHWHALS RVVELFCRNG EQNAAEKHLD
RAKEVNPRCV TESGYNVCRG RFEWYTGDQN EALRYYSRTK DSAAGWREKA LYYMIDICLN
PDNEIIIDEN SVENPETTIV EEASEQQKLA KYYLDLLGKL PTTDRFLLAQ NFIRMHTTDK
SAIQAALDEF NRMAFNADRS QVTNVGAVFG VARGHVLLKQ VQKAKTVLKM VNGRVWNFDD
SDYLEKCWLM LADIYINQNK NDQAVTFLDL VFKYNCNCLK AFELYGYMRE KEQKYVEAYK
MYEKAFMATK ERNPGFGYKL AFTYLKAKRL FACIETCQKV LDLNPQYPKI KKEIMDKAKA
LIRT
