TTC27_CAEEL
ID TTC27_CAEEL Reviewed; 771 AA.
AC P41842;
DT 01-NOV-1995, integrated into UniProtKB/Swiss-Prot.
DT 04-DEC-2007, sequence version 2.
DT 03-AUG-2022, entry version 145.
DE RecName: Full=Tetratricopeptide repeat-containing protein trd-1 {ECO:0000250|UniProtKB:Q6P3X3};
DE Short=TPR repeat protein 27 {ECO:0000250|UniProtKB:Q6P3X3};
DE AltName: Full=Tetratricopeptide repeat regulator of differentiation protein 1 {ECO:0000312|WormBase:T20B12.1};
GN Name=trd-1 {ECO:0000312|WormBase:T20B12.1};
GN ORFNames=T20B12.1 {ECO:0000312|WormBase:T20B12.1};
OS Caenorhabditis elegans.
OC Eukaryota; Metazoa; Ecdysozoa; Nematoda; Chromadorea; Rhabditida;
OC Rhabditina; Rhabditomorpha; Rhabditoidea; Rhabditidae; Peloderinae;
OC Caenorhabditis.
OX NCBI_TaxID=6239;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Bristol N2;
RX PubMed=9851916; DOI=10.1126/science.282.5396.2012;
RG The C. elegans sequencing consortium;
RT "Genome sequence of the nematode C. elegans: a platform for investigating
RT biology.";
RL Science 282:2012-2018(1998).
RN [2]
RP FUNCTION, SUBCELLULAR LOCATION, TISSUE SPECIFICITY, DEVELOPMENTAL STAGE,
RP AND DISRUPTION PHENOTYPE.
RX PubMed=25493563; DOI=10.1371/journal.pone.0114998;
RA Hughes S., Wilkinson H., Gilbert S.P., Kishida M., Ding S.S., Woollard A.;
RT "The C. elegans TPR containing protein, TRD-1, regulates cell fate choice
RT in the developing germ line and epidermis.";
RL PLoS ONE 9:E114998-E114998(2014).
CC -!- FUNCTION: Developmental protein required for cell fate determination in
CC both the germline and seam cells of the developing epidermis.
CC Specifically, involved in sex determination and may function in
CC parallel or downstream of other sex determination factors, including
CC tra-2 and fem-3, to promote oogenesis in its role in the regulation of
CC the switch from spermatogenesis to oogenesis in the gonads. Also
CC implicated in the mitosis to meiosis switch in distal tip cells.
CC {ECO:0000269|PubMed:25493563}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000269|PubMed:25493563}.
CC -!- TISSUE SPECIFICITY: Expressed in the spermatheca.
CC {ECO:0000269|PubMed:25493563}.
CC -!- DEVELOPMENTAL STAGE: Expressed in seam cells throughout postembryonic
CC development. Expressed in the developing gonad, distal tip cells and
CC spermatheca from larval stage L3. {ECO:0000269|PubMed:25493563}.
CC -!- DISRUPTION PHENOTYPE: High level of lethality with a significant
CC reduction in seam cell number. RNAi-mediated knockdown results in
CC defects linked to cell fate determination including impaired hypodermal
CC differentiation, fewer seam cells, possibly due to a change in cell
CC fate, a molting defect in 10% of animals and male tail abnormalities,
CC including reduced sensory ray number. The germline exhibits a mog
CC (masculinization of the germline) phenotype with increased sperm
CC numbers and largely absent oocytes in the gonad. Defective mitosis to
CC meiosis switching and general impaired morphology in distal tip cells
CC with abnormal extending processes (cytonemes) that are thicker and
CC extend further into the proximal gonad from early stages of
CC development. {ECO:0000269|PubMed:25493563}.
CC -!- SIMILARITY: Belongs to the TTC27 family. {ECO:0000305}.
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DR EMBL; FO081094; CCD69079.1; -; Genomic_DNA.
DR PIR; T16901; T16901.
DR RefSeq; NP_498636.2; NM_066235.5.
DR AlphaFoldDB; P41842; -.
DR SMR; P41842; -.
DR BioGRID; 41264; 22.
DR STRING; 6239.T20B12.1; -.
DR EPD; P41842; -.
DR PaxDb; P41842; -.
DR PeptideAtlas; P41842; -.
DR EnsemblMetazoa; T20B12.1.1; T20B12.1.1; WBGene00020600.
DR GeneID; 176055; -.
DR KEGG; cel:CELE_T20B12.1; -.
DR UCSC; T20B12.1; c. elegans.
DR CTD; 176055; -.
DR WormBase; T20B12.1; CE41454; WBGene00020600; trd-1.
DR eggNOG; KOG1128; Eukaryota.
DR GeneTree; ENSGT00500000044929; -.
DR HOGENOM; CLU_004905_2_0_1; -.
DR InParanoid; P41842; -.
DR OMA; VRYEPAH; -.
DR OrthoDB; 1039489at2759; -.
DR PhylomeDB; P41842; -.
DR PRO; PR:P41842; -.
DR Proteomes; UP000001940; Chromosome III.
DR Bgee; WBGene00020600; Expressed in germ line (C elegans) and 4 other tissues.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0030154; P:cell differentiation; IEA:UniProtKB-KW.
DR Gene3D; 1.25.40.10; -; 1.
DR InterPro; IPR002885; Pentatricopeptide_repeat.
DR InterPro; IPR011990; TPR-like_helical_dom_sf.
DR InterPro; IPR019734; TPR_repeat.
DR InterPro; IPR044244; TTC27/Emw1.
DR PANTHER; PTHR16193; PTHR16193; 1.
DR Pfam; PF01535; PPR; 2.
DR Pfam; PF13181; TPR_8; 1.
DR SMART; SM00028; TPR; 4.
DR SUPFAM; SSF48452; SSF48452; 1.
DR PROSITE; PS50005; TPR; 5.
DR PROSITE; PS50293; TPR_REGION; 1.
PE 2: Evidence at transcript level;
KW Cytoplasm; Differentiation; Reference proteome; Repeat; TPR repeat.
FT CHAIN 1..771
FT /note="Tetratricopeptide repeat-containing protein trd-1"
FT /evidence="ECO:0000305"
FT /id="PRO_0000106423"
FT REPEAT 389..415
FT /note="TPR 1"
FT REPEAT 416..449
FT /note="TPR 2"
FT REPEAT 451..484
FT /note="TPR 3"
FT REPEAT 485..518
FT /note="TPR 4"
FT REPEAT 520..552
FT /note="TPR 5"
FT REPEAT 553..586
FT /note="TPR 6"
SQ SEQUENCE 771 AA; 87973 MW; 2EEAD4E010335BA1 CRC64;
MIPADIIASI AGLVNEKDIE KITSEYQDNI WEFVESLVTH VLASNHVGYS DYNTSSFISE
ELARQKLSCG LNIPNAVSKS LWALHLAHEH VEKLKDLSDI PPIGYRVILE FYLVWQQLLI
DPEETIRTEI EPIMEKLRVL IAEENDAFTS EDRCQIQLEM AAVRFQFYEY DKADNLIKSA
SEECQLNMDL SGMMGKRTRF QQRDIAQLVL IHKDPSTTGT PLPPDSDIPQ SLDMNDDTLL
EQVAITEQGA RVDGRTLNAC QLSCLLWIAR HESATHRHDV LVHERCSPVL DTVIAARRYW
SIQAAALLAR AELERGRVRQ VDRSCTQSEL VVKLQQGVDD PVLIKDRLLR TSYILASGLT
PFWQSSVLLA GILNSLGCTS EALLILEKLE MWDGVIDCYK QLGQMDKAET LIRRLIEQKP
NDSMLHVYLG DITRNLEYFT KAIELSDDRN ARAHRSLGHL LLMDKKFEEA YKHLRRSLEL
QPIQLGTWFN AGYCAWKLEN FKESTQCYHR CVSLQPDHFE AWNNLSAAYI RHGQKPKAWK
LLQEALKYNY EHPNVWENYM LLSVDVGEFS QAIQAYHRLL DMNKRGADDE VLELIAQTLL
RREAEISMDE SEDKAQNEAE NRKEKEEMIK LLARISANHQ TLSPKTLRVY ALLKKPSVLS
SETRTEFEKY VRLLEKSLAA ANGKLTWPKE EKLALEVVET AVRLAEDRLE LAKFIASDTS
VKEASAKVRL SLRGILTRLD KDSGSRVSGD ETEKLQEIVE VAKSLLDSVA I
