TTC30_CAEEL
ID TTC30_CAEEL Reviewed; 656 AA.
AC Q8I7G4; Q8I7G3;
DT 20-MAY-2008, integrated into UniProtKB/Swiss-Prot.
DT 01-MAR-2003, sequence version 1.
DT 03-AUG-2022, entry version 118.
DE RecName: Full=Tetratricopeptide repeat protein 30 homolog;
DE Short=TPR repeat protein 30 homolog;
DE AltName: Full=Abnormal dye filling protein 1;
GN Name=dyf-1 {ECO:0000312|WormBase:F54C1.5a};
GN ORFNames=F54C1.5 {ECO:0000312|WormBase:F54C1.5a};
OS Caenorhabditis elegans.
OC Eukaryota; Metazoa; Ecdysozoa; Nematoda; Chromadorea; Rhabditida;
OC Rhabditina; Rhabditomorpha; Rhabditoidea; Rhabditidae; Peloderinae;
OC Caenorhabditis.
OX NCBI_TaxID=6239;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA], AND ALTERNATIVE SPLICING.
RC STRAIN=Bristol N2;
RX PubMed=9851916; DOI=10.1126/science.282.5396.2012;
RG The C. elegans sequencing consortium;
RT "Genome sequence of the nematode C. elegans: a platform for investigating
RT biology.";
RL Science 282:2012-2018(1998).
RN [2]
RP TISSUE SPECIFICITY.
RX PubMed=15916950; DOI=10.1016/j.cub.2005.04.059;
RA Blacque O.E., Perens E.A., Boroevich K.A., Inglis P.N., Li C., Warner A.,
RA Khattra J., Holt R.A., Ou G., Mah A.K., McKay S.J., Huang P., Swoboda P.,
RA Jones S.J.M., Marra M.A., Baillie D.L., Moerman D.G., Shaham S.,
RA Leroux M.R.;
RT "Functional genomics of the cilium, a sensory organelle.";
RL Curr. Biol. 15:935-941(2005).
RN [3]
RP FUNCTION, SUBCELLULAR LOCATION, AND DISRUPTION PHENOTYPE.
RX PubMed=16049494; DOI=10.1038/nature03818;
RA Ou G., Blacque O.E., Snow J.J., Leroux M.R., Scholey J.M.;
RT "Functional coordination of intraflagellar transport motors.";
RL Nature 436:583-587(2005).
RN [4]
RP FUNCTION.
RX PubMed=17761526; DOI=10.1091/mbc.e07-06-0537;
RA Pathak N.H., Obara T., Mangos S., Liu Y., Drummond I.A.;
RT "The zebrafish fleer gene encodes an essential regulator of cilia tubulin
RT polyglutamylation.";
RL Mol. Biol. Cell 18:4353-4364(2007).
RN [5]
RP FUNCTION, IDENTIFICATION IN IFT COMPLEX B, AND IDENTIFICATION BY MASS
RP SPECTROMETRY.
RX PubMed=28479320; DOI=10.1016/j.cub.2017.04.015;
RA Yi P., Li W.J., Dong M.Q., Ou G.;
RT "Dynein-driven retrograde intraflagellar transport is triphasic in C.
RT elegans sensory cilia.";
RL Curr. Biol. 27:1448-1461(2017).
CC -!- FUNCTION: Plays a role in anterograde intraflagellar transport (IFT),
CC the process by which cilia precursors are transported from the base of
CC the cilium to the site of their incorporation at the tip
CC (PubMed:16049494). Specifically required for the kinesin osm-3 to dock
CC onto and move the IFT particles which contain these precursors
CC (PubMed:16049494). Component of the intraflagellar transport (IFT)
CC complex B required for transport of proteins in the motile cilium
CC (PubMed:28479320). May be required for ciliary entrance and transport
CC of specific ciliary cargo proteins such as che-3 which are related to
CC motility (PubMed:28479320). Required for polyglutamylation of axonemal
CC tubulin in sensory cilia (PubMed:17761526).
CC {ECO:0000269|PubMed:16049494, ECO:0000269|PubMed:17761526,
CC ECO:0000269|PubMed:28479320}.
CC -!- SUBUNIT: Component of the IFT complex B composed of at least che-2,
CC che-13, dyf-1, dyf-3, dyf-6, dyf-11, dyf-13, ift-20, ift-74, ift-81,
CC ifta-2, osm-1, osm-5 and osm-6. {ECO:0000269|PubMed:28479320}.
CC -!- SUBCELLULAR LOCATION: Cell projection, cilium
CC {ECO:0000269|PubMed:16049494}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=a;
CC IsoId=Q8I7G4-1; Sequence=Displayed;
CC Name=b;
CC IsoId=Q8I7G4-2; Sequence=VSP_033490;
CC -!- TISSUE SPECIFICITY: Expressed in most amphid, both phasmid and several
CC labial-quadrant neurons. {ECO:0000269|PubMed:15916950}.
CC -!- DISRUPTION PHENOTYPE: Sensory neurons are unable to take up a
CC fluorescent dye from the media, a phenotype that is often associated
CC with aberrant sensory cilia. {ECO:0000269|PubMed:16049494}.
CC -!- SIMILARITY: Belongs to the TTC30/dfy-1/fleer family. {ECO:0000305}.
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; FO080890; CCD67546.1; -; Genomic_DNA.
DR EMBL; FO080890; CCD67547.1; -; Genomic_DNA.
DR RefSeq; NP_491494.2; NM_059093.5. [Q8I7G4-1]
DR RefSeq; NP_871863.1; NM_182063.4. [Q8I7G4-2]
DR AlphaFoldDB; Q8I7G4; -.
DR SMR; Q8I7G4; -.
DR BioGRID; 37583; 6.
DR ComplexPortal; CPX-1290; Intraflagellar transport complex B.
DR STRING; 6239.F54C1.5a; -.
DR PaxDb; Q8I7G4; -.
DR EnsemblMetazoa; F54C1.5a.1; F54C1.5a.1; WBGene00001117. [Q8I7G4-1]
DR EnsemblMetazoa; F54C1.5b.1; F54C1.5b.1; WBGene00001117. [Q8I7G4-2]
DR EnsemblMetazoa; F54C1.5b.2; F54C1.5b.2; WBGene00001117. [Q8I7G4-2]
DR EnsemblMetazoa; F54C1.5b.3; F54C1.5b.3; WBGene00001117. [Q8I7G4-2]
DR EnsemblMetazoa; F54C1.5b.4; F54C1.5b.4; WBGene00001117. [Q8I7G4-2]
DR GeneID; 172122; -.
DR KEGG; cel:CELE_F54C1.5; -.
DR UCSC; F54C1.5a; c. elegans. [Q8I7G4-1]
DR CTD; 172122; -.
DR WormBase; F54C1.5a; CE33108; WBGene00001117; dyf-1. [Q8I7G4-1]
DR WormBase; F54C1.5b; CE33109; WBGene00001117; dyf-1. [Q8I7G4-2]
DR eggNOG; KOG4340; Eukaryota.
DR GeneTree; ENSGT00390000010116; -.
DR InParanoid; Q8I7G4; -.
DR OMA; MRNHPEL; -.
DR OrthoDB; 247838at2759; -.
DR PhylomeDB; Q8I7G4; -.
DR Reactome; R-CEL-5620924; Intraflagellar transport.
DR PRO; PR:Q8I7G4; -.
DR Proteomes; UP000001940; Chromosome I.
DR Bgee; WBGene00001117; Expressed in pharyngeal muscle cell (C elegans) and 3 other tissues.
DR GO; GO:0005879; C:axonemal microtubule; IDA:UniProtKB.
DR GO; GO:0036064; C:ciliary basal body; IDA:MGI.
DR GO; GO:0005929; C:cilium; IDA:UniProtKB.
DR GO; GO:0030992; C:intraciliary transport particle B; IBA:GO_Central.
DR GO; GO:0120170; F:intraciliary transport particle B binding; IBA:GO_Central.
DR GO; GO:0042073; P:intraciliary transport; IMP:UniProtKB.
DR GO; GO:0072659; P:protein localization to plasma membrane; IMP:UniProtKB.
DR GO; GO:0018095; P:protein polyglutamylation; IMP:UniProtKB.
DR Gene3D; 1.25.40.10; -; 3.
DR InterPro; IPR011990; TPR-like_helical_dom_sf.
DR InterPro; IPR019734; TPR_repeat.
DR InterPro; IPR039941; TT30.
DR PANTHER; PTHR20931; PTHR20931; 1.
DR SMART; SM00028; TPR; 3.
DR SUPFAM; SSF48452; SSF48452; 2.
DR PROSITE; PS50293; TPR_REGION; 1.
PE 1: Evidence at protein level;
KW Alternative splicing; Cell projection; Cilium;
KW Cilium biogenesis/degradation; Reference proteome; Repeat; TPR repeat.
FT CHAIN 1..656
FT /note="Tetratricopeptide repeat protein 30 homolog"
FT /id="PRO_0000333210"
FT REPEAT 9..42
FT /note="TPR 1"
FT REPEAT 43..76
FT /note="TPR 2"
FT REPEAT 141..174
FT /note="TPR 3"
FT REPEAT 176..208
FT /note="TPR 4"
FT REPEAT 238..271
FT /note="TPR 5"
FT REPEAT 378..412
FT /note="TPR 6"
FT REPEAT 416..449
FT /note="TPR 7"
FT REPEAT 451..484
FT /note="TPR 8"
FT REPEAT 537..570
FT /note="TPR 9"
FT VAR_SEQ 1..59
FT /note="Missing (in isoform b)"
FT /evidence="ECO:0000305"
FT /id="VSP_033490"
SQ SEQUENCE 656 AA; 75607 MW; 6C10AACD65DC3C20 CRC64;
MNAMLNIKEG EFTSTIYTLI HEHKFNDAIR ILQYQHERNP KNLAALSLLA YCYYYTQDFM
NAADCYSQLS YNFPQYSQYK LYHAQSLYNA FRPADALAVV SMIQDENLLN ESVKLEAAIK
YQEDDLVNCR ILVEQLPEND AAVIINTACI DYKEGNYEEA LKKFNEATEF SGYQSGLAYS
IALCHYRRGD YDSALKLISE IINRGVKDHP EFNIGMVTEG IDVNFIQNTQ KLHESALIEA
FNLKFAIYYR TKDFKAAKES LTDMPPRNEH DADPITLHNL AISNANSDFG DSSAKLQFLL
GINPFPQETF ANLLFLYCKN DYFGLAADVL AENPSHTFYC LNEYQFNLLE ALIYMPTNPE
ESLKKLEKLE KECLDRLRKT AIEIQIKKEQ KTTDSDDSLE MRNLIESYDD SLEMYLPVLM
TYAKYYWDKR DYQAVEKLFR NSVDYCKEHD TWKLNVAHTI FMQEKKYKDA AAFYEPIVHK
KYDDGILEVP AMILANLVVC YIMTNQTDEA ELILKAVENE EEAALMMKPN EKFFHNSIIS
LVIGSLYCSK GNFEFGISRV VKALEPPEKK LGVDTWYYAK RCIVAAIELM AKNLLVMRDS
VVMEVIQFLT SCEVPGRNIY TVPDDLFEQA GESKVKCNVT YEARMIKAAL LMVFND
