TTC4_DROME
ID TTC4_DROME Reviewed; 396 AA.
AC A1Z6M6; Q6NMU5;
DT 18-SEP-2019, integrated into UniProtKB/Swiss-Prot.
DT 06-FEB-2007, sequence version 1.
DT 03-AUG-2022, entry version 128.
DE RecName: Full=DNA polymerase interacting tetratricopeptide repeat-containing, protein of 47 kDa {ECO:0000303|PubMed:11493638};
DE Short=DNA polymerase interacting TPR-containing, protein of 47 kDa {ECO:0000303|PubMed:11493638};
DE AltName: Full=Tetratricopeptide repeat protein 4 homolog;
DE Short=TPR repeat protein 4 homolog;
GN Name=Dpit47; ORFNames=CG3189;
OS Drosophila melanogaster (Fruit fly).
OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea;
OC Drosophilidae; Drosophila; Sophophora.
OX NCBI_TaxID=7227;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Berkeley;
RX PubMed=10731132; DOI=10.1126/science.287.5461.2185;
RA Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D.,
RA Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F.,
RA George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N.,
RA Sutton G.G., Wortman J.R., Yandell M.D., Zhang Q., Chen L.X., Brandon R.C.,
RA Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C.,
RA Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A.,
RA An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A.,
RA Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V.,
RA Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J.,
RA Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E.,
RA Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B.,
RA Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I.,
RA Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C.,
RA Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S.,
RA Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M.,
RA Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M.,
RA Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D.,
RA Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F.,
RA Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D.,
RA Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A.,
RA Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C.,
RA McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C.,
RA Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L.,
RA Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R.,
RA Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V.,
RA Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F.,
RA Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J.,
RA Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R.,
RA Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y.,
RA Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T.,
RA Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S.,
RA Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W.,
RA Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M.,
RA Venter J.C.;
RT "The genome sequence of Drosophila melanogaster.";
RL Science 287:2185-2195(2000).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=Berkeley;
RX PubMed=12537572; DOI=10.1186/gb-2002-3-12-research0083;
RA Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S.,
RA Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E.,
RA Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P.,
RA Bettencourt B.R., Celniker S.E., de Grey A.D.N.J., Drysdale R.A.,
RA Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M.,
RA Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E.;
RT "Annotation of the Drosophila melanogaster euchromatic genome: a systematic
RT review.";
RL Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=Berkeley; TISSUE=Embryo;
RX PubMed=12537569; DOI=10.1186/gb-2002-3-12-research0080;
RA Stapleton M., Carlson J.W., Brokstein P., Yu C., Champe M., George R.A.,
RA Guarin H., Kronmiller B., Pacleb J.M., Park S., Wan K.H., Rubin G.M.,
RA Celniker S.E.;
RT "A Drosophila full-length cDNA resource.";
RL Genome Biol. 3:RESEARCH0080.1-RESEARCH0080.8(2002).
RN [4]
RP FUNCTION, INTERACTION WITH HSP83; HSP70AA AND DNAPOL-ALPHA180, AND
RP SUBCELLULAR LOCATION.
RX PubMed=11493638; DOI=10.1242/jcs.114.11.2015;
RA Crevel G., Bates H., Huikeshoven H., Cotterill S.;
RT "The Drosophila Dpit47 protein is a nuclear Hsp90 co-chaperone that
RT interacts with DNA polymerase alpha.";
RL J. Cell Sci. 114:2015-2025(2001).
CC -!- FUNCTION: May act as a co-chaperone for HSP83.
CC {ECO:0000269|PubMed:11493638}.
CC -!- SUBUNIT: Forms a complex with Hsp83 and Hsp70aa (PubMed:11493638).
CC Interacts with DNApol-alpha180; the interaction inhibits the activity
CC of the DNA polymerase and occurs only in proliferating cells but not in
CC quiescent cells (PubMed:11493638). {ECO:0000269|PubMed:11493638}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000269|PubMed:11493638}. Nucleus,
CC nucleoplasm {ECO:0000269|PubMed:11493638}. Cytoplasm
CC {ECO:0000269|PubMed:11493638}. Note=In embryos prior to
CC cellularisation, found in the nucleus and cytoplasm but after
CC cellularisation seen predominantly in the nucleus.
CC {ECO:0000269|PubMed:11493638}.
CC -!- TISSUE SPECIFICITY: More abundant in young embryos, pupae and females
CC and a lower level expression seen in late embryos, larvae and males.
CC {ECO:0000269|PubMed:11493638}.
CC -!- SIMILARITY: Belongs to the TTC4 family. {ECO:0000305}.
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DR EMBL; AE013599; AAF57368.2; -; Genomic_DNA.
DR EMBL; AE013599; AGB93262.1; -; Genomic_DNA.
DR EMBL; BT132984; AEX31654.1; -; mRNA.
DR EMBL; BT011560; AAS15696.1; -; mRNA.
DR RefSeq; NP_001260729.1; NM_001273800.1.
DR RefSeq; NP_525106.2; NM_080367.3.
DR AlphaFoldDB; A1Z6M6; -.
DR SMR; A1Z6M6; -.
DR IntAct; A1Z6M6; 5.
DR STRING; 7227.FBpp0085479; -.
DR PaxDb; A1Z6M6; -.
DR PRIDE; A1Z6M6; -.
DR DNASU; 35565; -.
DR EnsemblMetazoa; FBtr0086146; FBpp0085479; FBgn0266518.
DR EnsemblMetazoa; FBtr0336664; FBpp0307645; FBgn0266518.
DR GeneID; 35565; -.
DR KEGG; dme:Dmel_CG3189; -.
DR UCSC; CG3189-RA; d. melanogaster.
DR CTD; 35565; -.
DR FlyBase; FBgn0266518; Dpit47.
DR VEuPathDB; VectorBase:FBgn0266518; -.
DR eggNOG; KOG0551; Eukaryota.
DR GeneTree; ENSGT00510000049371; -.
DR HOGENOM; CLU_040446_2_0_1; -.
DR InParanoid; A1Z6M6; -.
DR OMA; HPMFEGL; -.
DR OrthoDB; 866891at2759; -.
DR PhylomeDB; A1Z6M6; -.
DR BioGRID-ORCS; 35565; 0 hits in 1 CRISPR screen.
DR GenomeRNAi; 35565; -.
DR PRO; PR:A1Z6M6; -.
DR Proteomes; UP000000803; Chromosome 2R.
DR Bgee; FBgn0266518; Expressed in eye disc (Drosophila) and 23 other tissues.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0005654; C:nucleoplasm; IDA:UniProtKB.
DR GO; GO:0005634; C:nucleus; IDA:FlyBase.
DR GO; GO:0030544; F:Hsp70 protein binding; IDA:UniProtKB.
DR GO; GO:0051879; F:Hsp90 protein binding; IDA:UniProtKB.
DR GO; GO:0006457; P:protein folding; IBA:GO_Central.
DR Gene3D; 1.25.40.10; -; 1.
DR InterPro; IPR044059; Csn1/TTC4_wheel.
DR InterPro; IPR011990; TPR-like_helical_dom_sf.
DR InterPro; IPR019734; TPR_repeat.
DR Pfam; PF18972; Wheel; 1.
DR SMART; SM00028; TPR; 3.
DR SUPFAM; SSF48452; SSF48452; 1.
DR PROSITE; PS50293; TPR_REGION; 1.
PE 1: Evidence at protein level;
KW Cytoplasm; Nucleus; Reference proteome; Repeat; TPR repeat.
FT CHAIN 1..396
FT /note="DNA polymerase interacting tetratricopeptide repeat-
FT containing, protein of 47 kDa"
FT /id="PRO_0000447984"
FT REPEAT 91..124
FT /note="TPR 1"
FT REPEAT 129..162
FT /note="TPR 2"
FT REPEAT 163..196
FT /note="TPR 3"
FT CONFLICT 200
FT /note="I -> S (in Ref. 3; AAS15696)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 396 AA; 46906 MW; B1115ED0F708D7FF CRC64;
MKQMAAQKAW TDEERLELAA QLDAELDAFI DGLEKKRYEE GWPEDRWQEE MDKHPFFMKR
APQPGDDVHP MFEGLQKLKY DPEENTRDEL ALNYKEDGNF YMKHKKFRMA IYSFTEGIKT
KTDNPDVLAV LYNNRSAAHF FIKNYRSSLS DAQRALFYKP DYTKARWRSA QCAYELERFD
LCTQMCEELL EVDVDNEVAI ALLHKNKMKK LEIERNQRKE AAEAKRRLTR FHRLRDAIEQ
RAIKFDDQKV GKKDVLSEEL LYPKFLPLED HPVHLDEDGS TLIWPAAFSY PEFLYSDFYQ
QLPETTTMRD CLATLLTEKL PYDKAHNYRL GNVHVYYENR KVGCVHKVDE EKQLAEIIAE
KGFFVSGGAL LFYVVHKDSR VEQEFINERR RPMVYS
