C4BPA_MOUSE
ID C4BPA_MOUSE Reviewed; 469 AA.
AC P08607; Q91X48;
DT 01-AUG-1988, integrated into UniProtKB/Swiss-Prot.
DT 27-JUL-2011, sequence version 3.
DT 03-AUG-2022, entry version 164.
DE RecName: Full=C4b-binding protein;
DE Short=C4bp;
DE Flags: Precursor;
GN Name=C4bpa; Synonyms=C4bp;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA].
RX PubMed=3663616; DOI=10.1021/bi00389a012;
RA Kristensen T., Ogata R.T., Chung L.P., Reid K.B.M., Tack B.F.;
RT "cDNA structure of murine C4b-binding protein, a regulatory component of
RT the serum complement system.";
RL Biochemistry 26:4668-4674(1987).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=C57BL/6J; TISSUE=Liver;
RX PubMed=16141072; DOI=10.1126/science.1112014;
RA Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N.,
RA Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K.,
RA Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.,
RA Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R.,
RA Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T.,
RA Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A.,
RA Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B.,
RA Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M.,
RA Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S.,
RA Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E.,
RA Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D.,
RA Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M.,
RA Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H.,
RA Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V.,
RA Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S.,
RA Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H.,
RA Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N.,
RA Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F.,
RA Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G.,
RA Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z.,
RA Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C.,
RA Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y.,
RA Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S.,
RA Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K.,
RA Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R.,
RA van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H.,
RA Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M.,
RA Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C.,
RA Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S.,
RA Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K.,
RA Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M.,
RA Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C.,
RA Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A.,
RA Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.;
RT "The transcriptional landscape of the mammalian genome.";
RL Science 309:1559-1563(2005).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RA Mural R.J., Adams M.D., Myers E.W., Smith H.O., Venter J.C.;
RL Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases.
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=FVB/N; TISSUE=Liver;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [5]
RP GLYCOSYLATION [LARGE SCALE ANALYSIS] AT ASN-275.
RC STRAIN=C57BL/6J; TISSUE=Plasma;
RX PubMed=17330941; DOI=10.1021/pr0604559;
RA Bernhard O.K., Kapp E.A., Simpson R.J.;
RT "Enhanced analysis of the mouse plasma proteome using cysteine-containing
RT tryptic glycopeptides.";
RL J. Proteome Res. 6:987-995(2007).
RN [6]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Brown adipose tissue, and Heart;
RX PubMed=21183079; DOI=10.1016/j.cell.2010.12.001;
RA Huttlin E.L., Jedrychowski M.P., Elias J.E., Goswami T., Rad R.,
RA Beausoleil S.A., Villen J., Haas W., Sowa M.E., Gygi S.P.;
RT "A tissue-specific atlas of mouse protein phosphorylation and expression.";
RL Cell 143:1174-1189(2010).
CC -!- FUNCTION: Controls the classical pathway of complement activation. It
CC binds as a cofactor to C3b/C4b inactivator (C3bINA), which then
CC hydrolyzes the complement fragment C4b. It also accelerates the
CC degradation of the C4bC2a complex (C3 convertase) by dissociating the
CC complement fragment C2a. Alpha chain binds C4b. It interacts also with
CC serum amyloid P component.
CC -!- SUBUNIT: Homoheptamer; not covalently linked. Mouse lacks the beta
CC chain of C4BP.
CC -!- INTERACTION:
CC P08607; Q13873: BMPR2; Xeno; NbExp=3; IntAct=EBI-527325, EBI-527196;
CC -!- SUBCELLULAR LOCATION: Secreted.
CC -!- CAUTION: It is uncertain whether Met-1 or Met-44 is the initiator.
CC {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAA37312.1; Type=Erroneous initiation; Note=Truncated N-terminus.; Evidence={ECO:0000305};
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DR EMBL; M17122; AAA37312.1; ALT_INIT; mRNA.
DR EMBL; AK149468; BAE28899.1; -; mRNA.
DR EMBL; AK149477; BAE28904.1; -; mRNA.
DR EMBL; CH466520; EDL39738.1; -; Genomic_DNA.
DR EMBL; BC012257; AAH12257.1; -; mRNA.
DR CCDS; CCDS35700.1; -.
DR PIR; A27117; NBMSC4.
DR RefSeq; NP_031602.3; NM_007576.3.
DR RefSeq; XP_011246208.1; XM_011247906.2.
DR AlphaFoldDB; P08607; -.
DR SMR; P08607; -.
DR IntAct; P08607; 1.
DR STRING; 10090.ENSMUSP00000027657; -.
DR GlyConnect; 814; 1 N-Linked glycan (1 site).
DR GlyGen; P08607; 7 sites, 2 N-linked glycans (1 site).
DR iPTMnet; P08607; -.
DR PhosphoSitePlus; P08607; -.
DR CPTAC; non-CPTAC-3318; -.
DR MaxQB; P08607; -.
DR PaxDb; P08607; -.
DR PeptideAtlas; P08607; -.
DR PRIDE; P08607; -.
DR Antibodypedia; 694; 300 antibodies from 29 providers.
DR DNASU; 12269; -.
DR Ensembl; ENSMUST00000027657; ENSMUSP00000027657; ENSMUSG00000026405.
DR GeneID; 12269; -.
DR KEGG; mmu:12269; -.
DR UCSC; uc007cmc.1; mouse.
DR CTD; 12269; -.
DR MGI; MGI:88229; C4bp.
DR VEuPathDB; HostDB:ENSMUSG00000026405; -.
DR eggNOG; ENOG502SHRK; Eukaryota.
DR GeneTree; ENSGT00940000154640; -.
DR HOGENOM; CLU_020107_5_2_1; -.
DR InParanoid; P08607; -.
DR OMA; WKPQIPS; -.
DR OrthoDB; 1058295at2759; -.
DR PhylomeDB; P08607; -.
DR TreeFam; TF334137; -.
DR BioGRID-ORCS; 12269; 2 hits in 72 CRISPR screens.
DR PRO; PR:P08607; -.
DR Proteomes; UP000000589; Chromosome 1.
DR RNAct; P08607; protein.
DR Bgee; ENSMUSG00000026405; Expressed in left lobe of liver and 41 other tissues.
DR ExpressionAtlas; P08607; baseline and differential.
DR Genevisible; P08607; MM.
DR GO; GO:0005615; C:extracellular space; ISO:MGI.
DR GO; GO:0006958; P:complement activation, classical pathway; IEA:UniProtKB-KW.
DR GO; GO:0045087; P:innate immune response; IEA:UniProtKB-KW.
DR GO; GO:0045959; P:negative regulation of complement activation, classical pathway; ISO:MGI.
DR GO; GO:0045732; P:positive regulation of protein catabolic process; ISO:MGI.
DR GO; GO:1903027; P:regulation of opsonization; ISO:MGI.
DR GO; GO:0009609; P:response to symbiotic bacterium; ISO:MGI.
DR CDD; cd00033; CCP; 6.
DR InterPro; IPR040514; C4bp_oligo.
DR InterPro; IPR035976; Sushi/SCR/CCP_sf.
DR InterPro; IPR000436; Sushi_SCR_CCP_dom.
DR Pfam; PF18453; C4bp_oligo; 1.
DR Pfam; PF00084; Sushi; 6.
DR SMART; SM00032; CCP; 6.
DR SUPFAM; SSF57535; SSF57535; 6.
DR PROSITE; PS50923; SUSHI; 6.
PE 1: Evidence at protein level;
KW Complement pathway; Disulfide bond; Glycoprotein; Immunity;
KW Innate immunity; Reference proteome; Repeat; Secreted; Signal; Sushi.
FT SIGNAL 1..56
FT CHAIN 57..469
FT /note="C4b-binding protein"
FT /id="PRO_0000005889"
FT DOMAIN 57..117
FT /note="Sushi 1"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00302"
FT DOMAIN 118..178
FT /note="Sushi 2"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00302"
FT DOMAIN 179..242
FT /note="Sushi 3"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00302"
FT DOMAIN 243..301
FT /note="Sushi 4"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00302"
FT DOMAIN 302..357
FT /note="Sushi 5"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00302"
FT DOMAIN 358..415
FT /note="Sushi 6"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00302"
FT CARBOHYD 74
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 227
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 275
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000269|PubMed:17330941"
FT CARBOHYD 292
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 366
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 381
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 428
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT DISULFID 58..103
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00302"
FT DISULFID 88..115
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00302"
FT DISULFID 120..160
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00302"
FT DISULFID 146..176
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00302"
FT DISULFID 181..223
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00302"
FT DISULFID 209..240
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00302"
FT DISULFID 245..287
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00302"
FT DISULFID 273..299
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00302"
FT DISULFID 303..343
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00302"
FT DISULFID 329..355
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00302"
FT DISULFID 359..400
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00302"
FT DISULFID 386..413
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00302"
SQ SEQUENCE 469 AA; 51524 MW; 14266B31430F7107 CRC64;
MCAKQQQTLL PTRAAHGRLH RNRDAVAWPF STLCRVSGPT LFQMTFTAAL WVAVFGKCGP
PPAIPNALPA SDVNRTDFES HTTLKYECLP GYGRGISRMM VYCKPSGEWE ISVSCAKKHC
RNPGYLDNGY VNGETITFGS QIEFSCQEGF ILVGSSTSSC EVRGKGVAWS NPFPECVIVK
CGPPPDISNG KHSGTEDFYP YNHGISYTCD PGFRLVGSPF IGCTVVNKTV PVWSSSPPTC
EKIICSQPNI LHGVIVSGYK ATYTHRDSVR LACLNGTVLR GRHVIECQGN GNWSSLPTCE
FDCDLPPAIV NGYYTSMVYS KITLVTYECD KGYRLVGKAI ISCSFSKWKG TAPQCKALCQ
KPEVGNGTLS DEKDQYVESE NVTIQCDSGF AMLGSQSISC SESGTWYPEV PRCEQEASED
LKPALTGNKT MQYVPNSHDV KMALEIYKLT LEVELLQLQI QKEKHTEAH
