TTC4_MOUSE
ID TTC4_MOUSE Reviewed; 386 AA.
AC Q8R3H9;
DT 19-SEP-2002, integrated into UniProtKB/Swiss-Prot.
DT 01-JUN-2002, sequence version 1.
DT 03-AUG-2022, entry version 131.
DE RecName: Full=Tetratricopeptide repeat protein 4;
DE Short=TPR repeat protein 4;
GN Name=Ttc4;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [2]
RP INTERACTION WITH MSL1, AND SUBCELLULAR LOCATION.
RX PubMed=17335777; DOI=10.1016/j.bbrc.2007.02.073;
RA Dmitriev R.I., Korneenko T.V., Bessonov A.A., Shakhparonov M.I.,
RA Modyanov N.N., Pestov N.B.;
RT "Characterization of hampin/MSL1 as a node in the nuclear interactome.";
RL Biochem. Biophys. Res. Commun. 355:1051-1057(2007).
RN [3]
RP SUBCELLULAR LOCATION, AND TISSUE SPECIFICITY.
RX PubMed=19390865; DOI=10.1007/s00441-009-0785-y;
RA Dmitriev R.I., Okkelman I.A., Abdulin R.A., Shakhparonov M.I., Pestov N.B.;
RT "Nuclear transport of protein TTC4 depends on the cell cycle.";
RL Cell Tissue Res. 336:521-527(2009).
RN [4]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Spleen, and Testis;
RX PubMed=21183079; DOI=10.1016/j.cell.2010.12.001;
RA Huttlin E.L., Jedrychowski M.P., Elias J.E., Goswami T., Rad R.,
RA Beausoleil S.A., Villen J., Haas W., Sowa M.E., Gygi S.P.;
RT "A tissue-specific atlas of mouse protein phosphorylation and expression.";
RL Cell 143:1174-1189(2010).
RN [5]
RP SUBCELLULAR LOCATION.
RX PubMed=24913909; DOI=10.1002/jcb.24868;
RA Dmitriev R.I., Pestov N.B., Shakhparonov M.I., Okkelman I.A.;
RT "Two distinct nuclear localization signals in mammalian MSL1 regulate its
RT function.";
RL J. Cell. Biochem. 115:1967-1973(2014).
CC -!- FUNCTION: May act as a co-chaperone for HSP90AB1 (By similarity).
CC {ECO:0000250|UniProtKB:O95801}.
CC -!- SUBUNIT: Interacts (via TPR repeats) with HSP90AB1 (By similarity).
CC Interacts with HSPA8, CDC6 and TBK1 (By similarity). Interacts with
CC isoform 1 and isoform 3 of MSL1 (PubMed:17335777).
CC {ECO:0000250|UniProtKB:O95801, ECO:0000269|PubMed:17335777}.
CC -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000269|PubMed:17335777,
CC ECO:0000269|PubMed:19390865, ECO:0000269|PubMed:24913909}. Nucleus,
CC nucleoplasm {ECO:0000250|UniProtKB:O95801}. Cytoplasm
CC {ECO:0000269|PubMed:19390865}. Note=Predominantly nuclear in the G1 and
CC S phases of cell cycle and is evenly distributed between the nucleus
CC and cytoplasm in the G2 phase (PubMed:19390865). MSL1 can promote its
CC nuclear localization (PubMed:19390865, PubMed:24913909).
CC {ECO:0000269|PubMed:19390865, ECO:0000269|PubMed:24913909}.
CC -!- TISSUE SPECIFICITY: Expressed at high levels in the heart, testis,
CC kidney, brain and tongue (PubMed:19390865). Expressed at low levels in
CC the stomach, lung and liver (PubMed:19390865).
CC {ECO:0000269|PubMed:19390865}.
CC -!- SIMILARITY: Belongs to the TTC4 family. {ECO:0000305}.
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DR EMBL; BC025435; AAH25435.1; -; mRNA.
DR CCDS; CCDS18424.1; -.
DR RefSeq; NP_082485.1; NM_028209.2.
DR AlphaFoldDB; Q8R3H9; -.
DR SMR; Q8R3H9; -.
DR BioGRID; 215330; 38.
DR STRING; 10090.ENSMUSP00000026480; -.
DR iPTMnet; Q8R3H9; -.
DR PhosphoSitePlus; Q8R3H9; -.
DR EPD; Q8R3H9; -.
DR MaxQB; Q8R3H9; -.
DR PaxDb; Q8R3H9; -.
DR PeptideAtlas; Q8R3H9; -.
DR PRIDE; Q8R3H9; -.
DR ProteomicsDB; 297749; -.
DR Antibodypedia; 34867; 78 antibodies from 20 providers.
DR Ensembl; ENSMUST00000026480; ENSMUSP00000026480; ENSMUSG00000025413.
DR GeneID; 72354; -.
DR KEGG; mmu:72354; -.
DR UCSC; uc008tyr.2; mouse.
DR CTD; 7268; -.
DR MGI; MGI:1919604; Ttc4.
DR VEuPathDB; HostDB:ENSMUSG00000025413; -.
DR eggNOG; KOG0551; Eukaryota.
DR GeneTree; ENSGT00510000049371; -.
DR HOGENOM; CLU_040446_2_0_1; -.
DR InParanoid; Q8R3H9; -.
DR OMA; HPMFEGL; -.
DR OrthoDB; 866891at2759; -.
DR PhylomeDB; Q8R3H9; -.
DR TreeFam; TF314657; -.
DR BioGRID-ORCS; 72354; 23 hits in 72 CRISPR screens.
DR PRO; PR:Q8R3H9; -.
DR Proteomes; UP000000589; Chromosome 4.
DR RNAct; Q8R3H9; protein.
DR Bgee; ENSMUSG00000025413; Expressed in spermatocyte and 246 other tissues.
DR ExpressionAtlas; Q8R3H9; baseline and differential.
DR Genevisible; Q8R3H9; MM.
DR GO; GO:0005737; C:cytoplasm; IDA:UniProtKB.
DR GO; GO:0005654; C:nucleoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0005634; C:nucleus; IDA:UniProtKB.
DR GO; GO:0030544; F:Hsp70 protein binding; IBA:GO_Central.
DR GO; GO:0051879; F:Hsp90 protein binding; IBA:GO_Central.
DR GO; GO:0051607; P:defense response to virus; ISS:UniProtKB.
DR GO; GO:0045087; P:innate immune response; ISS:UniProtKB.
DR GO; GO:0006457; P:protein folding; IBA:GO_Central.
DR Gene3D; 1.25.40.10; -; 1.
DR InterPro; IPR044059; Csn1/TTC4_wheel.
DR InterPro; IPR011990; TPR-like_helical_dom_sf.
DR InterPro; IPR019734; TPR_repeat.
DR Pfam; PF18972; Wheel; 1.
DR SMART; SM00028; TPR; 3.
DR SUPFAM; SSF48452; SSF48452; 1.
DR PROSITE; PS50293; TPR_REGION; 1.
PE 1: Evidence at protein level;
KW Acetylation; Antiviral defense; Cytoplasm; Immunity; Innate immunity;
KW Nucleus; Phosphoprotein; Reference proteome; Repeat; TPR repeat.
FT CHAIN 1..386
FT /note="Tetratricopeptide repeat protein 4"
FT /id="PRO_0000106380"
FT REPEAT 79..112
FT /note="TPR 1"
FT REPEAT 117..150
FT /note="TPR 2"
FT REPEAT 151..184
FT /note="TPR 3"
FT SITE 77
FT /note="Essential for interaction with CDC6"
FT /evidence="ECO:0000250|UniProtKB:O95801"
FT SITE 152
FT /note="Essential for interaction with HSPA8"
FT /evidence="ECO:0000250|UniProtKB:O95801"
FT SITE 156
FT /note="Essential for interaction with HSPA8"
FT /evidence="ECO:0000250|UniProtKB:O95801"
FT MOD_RES 1
FT /note="N-acetylmethionine"
FT /evidence="ECO:0000250|UniProtKB:O95801"
FT MOD_RES 51
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:O95801"
FT MOD_RES 244
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:O95801"
SQ SEQUENCE 386 AA; 44339 MW; 8343EDDBAB57E710 CRC64;
MESSEPEPTE DASMDAFLEK FQSQPYRGGF REDQWEEEFD KIPLFMKKAP SEIDPEEFPD
LACLQSMIFD DDRYPEEQAK TYKDEGNDYF KEKDYKKAVL SYSEGLKKKC ADPDLNAVLY
TNRAAAQYYL GNVRSSLNDV LAAKKLKPGH LKAIIRGALC HLELKHFAEA VNWCDEGLQI
DAKEKKLLEI RAKADKLKRM EERDLRKAKL KEKKEQHQNE ALLQAIKARN IRLVSESAGE
DEDSASNGPA EILLDGLSSE NPYGARLSID DQGRLSWPVL FLYPEYAQSD FISAFHEDTR
FIDHLMAMFS EAPSWDSEHK YHPENLEVYF EDEDRAELYQ VSPDSTLLQV LQHPRCCVKA
LTPAFLVCVG SSPFCRNYLQ GKKVHR
