C4G15_DROME
ID C4G15_DROME Reviewed; 574 AA.
AC Q9VYY4;
DT 01-DEC-2000, integrated into UniProtKB/Swiss-Prot.
DT 01-MAY-2000, sequence version 1.
DT 03-AUG-2022, entry version 167.
DE RecName: Full=Cytochrome P450 4g15;
DE EC=1.14.-.-;
DE AltName: Full=CYPIVG15;
GN Name=Cyp4g15; ORFNames=CG11715;
OS Drosophila melanogaster (Fruit fly).
OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea;
OC Drosophilidae; Drosophila; Sophophora.
OX NCBI_TaxID=7227;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, TISSUE SPECIFICITY, AND DEVELOPMENTAL
RP STAGE.
RC STRAIN=Oregon-R; TISSUE=Larva;
RX PubMed=10891384; DOI=10.1006/bbrc.2000.3058;
RA Maibeche-Coisne M., Monti-Dedieu L., Aragon S., Dauphin-Villemant C.;
RT "A new cytochrome P450 from Drosophila melanogaster, CYP4G15, expressed in
RT the nervous system.";
RL Biochem. Biophys. Res. Commun. 273:1132-1137(2000).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Berkeley;
RX PubMed=10731132; DOI=10.1126/science.287.5461.2185;
RA Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D.,
RA Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F.,
RA George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N.,
RA Sutton G.G., Wortman J.R., Yandell M.D., Zhang Q., Chen L.X., Brandon R.C.,
RA Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C.,
RA Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A.,
RA An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A.,
RA Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V.,
RA Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J.,
RA Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E.,
RA Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B.,
RA Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I.,
RA Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C.,
RA Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S.,
RA Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M.,
RA Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M.,
RA Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D.,
RA Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F.,
RA Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D.,
RA Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A.,
RA Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C.,
RA McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C.,
RA Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L.,
RA Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R.,
RA Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V.,
RA Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F.,
RA Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J.,
RA Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R.,
RA Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y.,
RA Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T.,
RA Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S.,
RA Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W.,
RA Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M.,
RA Venter J.C.;
RT "The genome sequence of Drosophila melanogaster.";
RL Science 287:2185-2195(2000).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=Berkeley;
RX PubMed=12537572; DOI=10.1186/gb-2002-3-12-research0083;
RA Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S.,
RA Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E.,
RA Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P.,
RA Bettencourt B.R., Celniker S.E., de Grey A.D.N.J., Drysdale R.A.,
RA Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M.,
RA Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E.;
RT "Annotation of the Drosophila melanogaster euchromatic genome: a systematic
RT review.";
RL Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=Berkeley; TISSUE=Head;
RX PubMed=12537569; DOI=10.1186/gb-2002-3-12-research0080;
RA Stapleton M., Carlson J.W., Brokstein P., Yu C., Champe M., George R.A.,
RA Guarin H., Kronmiller B., Pacleb J.M., Park S., Wan K.H., Rubin G.M.,
RA Celniker S.E.;
RT "A Drosophila full-length cDNA resource.";
RL Genome Biol. 3:RESEARCH0080.1-RESEARCH0080.8(2002).
CC -!- FUNCTION: Probably involved in steroid hormones biosynthesis.
CC {ECO:0000269|PubMed:10891384}.
CC -!- COFACTOR:
CC Name=heme; Xref=ChEBI:CHEBI:30413; Evidence={ECO:0000250};
CC -!- SUBCELLULAR LOCATION: Endoplasmic reticulum membrane {ECO:0000305};
CC Peripheral membrane protein {ECO:0000305}. Microsome membrane
CC {ECO:0000305}; Peripheral membrane protein {ECO:0000305}.
CC -!- TISSUE SPECIFICITY: Expressed in larval brain cortex cells and ring
CC glands and weakly in larval digestive system and adult nervous system.
CC {ECO:0000269|PubMed:10891384}.
CC -!- DEVELOPMENTAL STAGE: Expressed throughout development.
CC {ECO:0000269|PubMed:10891384}.
CC -!- SIMILARITY: Belongs to the cytochrome P450 family. {ECO:0000305}.
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; AF159624; AAF76522.1; -; mRNA.
DR EMBL; AE014298; AAF48049.1; -; Genomic_DNA.
DR EMBL; AY060719; AAL28267.1; -; mRNA.
DR PIR; JC7327; JC7327.
DR RefSeq; NP_572721.1; NM_132493.3.
DR RefSeq; NP_727531.2; NM_167287.2.
DR AlphaFoldDB; Q9VYY4; -.
DR SMR; Q9VYY4; -.
DR BioGRID; 58504; 4.
DR IntAct; Q9VYY4; 3.
DR STRING; 7227.FBpp0073352; -.
DR PaxDb; Q9VYY4; -.
DR DNASU; 32093; -.
DR EnsemblMetazoa; FBtr0073503; FBpp0073352; FBgn0030304.
DR EnsemblMetazoa; FBtr0346268; FBpp0312025; FBgn0030304.
DR GeneID; 32093; -.
DR KEGG; dme:Dmel_CG11715; -.
DR UCSC; CG11715-RA; d. melanogaster.
DR CTD; 32093; -.
DR FlyBase; FBgn0030304; Cyp4g15.
DR VEuPathDB; VectorBase:FBgn0030304; -.
DR eggNOG; KOG0157; Eukaryota.
DR HOGENOM; CLU_001570_5_1_1; -.
DR InParanoid; Q9VYY4; -.
DR OMA; EKTANRH; -.
DR OrthoDB; 1247045at2759; -.
DR PhylomeDB; Q9VYY4; -.
DR Reactome; R-DME-193144; Estrogen biosynthesis.
DR Reactome; R-DME-211976; Endogenous sterols.
DR BioGRID-ORCS; 32093; 0 hits in 1 CRISPR screen.
DR GenomeRNAi; 32093; -.
DR PRO; PR:Q9VYY4; -.
DR Proteomes; UP000000803; Chromosome X.
DR Bgee; FBgn0030304; Expressed in head capsule and 21 other tissues.
DR ExpressionAtlas; Q9VYY4; baseline and differential.
DR Genevisible; Q9VYY4; DM.
DR GO; GO:0005789; C:endoplasmic reticulum membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0020037; F:heme binding; IEA:InterPro.
DR GO; GO:0005506; F:iron ion binding; IEA:InterPro.
DR GO; GO:0004497; F:monooxygenase activity; IEA:UniProtKB-KW.
DR GO; GO:0016705; F:oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen; IEA:InterPro.
DR Gene3D; 1.10.630.10; -; 2.
DR InterPro; IPR001128; Cyt_P450.
DR InterPro; IPR017972; Cyt_P450_CS.
DR InterPro; IPR002401; Cyt_P450_E_grp-I.
DR InterPro; IPR036396; Cyt_P450_sf.
DR Pfam; PF00067; p450; 2.
DR PRINTS; PR00463; EP450I.
DR PRINTS; PR00385; P450.
DR SUPFAM; SSF48264; SSF48264; 1.
DR PROSITE; PS00086; CYTOCHROME_P450; 1.
PE 2: Evidence at transcript level;
KW Endoplasmic reticulum; Heme; Iron; Membrane; Metal-binding; Microsome;
KW Monooxygenase; Oxidoreductase; Reference proteome.
FT CHAIN 1..574
FT /note="Cytochrome P450 4g15"
FT /id="PRO_0000051844"
FT REGION 288..327
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 303..318
FT /note="Basic and acidic residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT BINDING 379
FT /ligand="heme"
FT /ligand_id="ChEBI:CHEBI:30413"
FT /note="covalent"
FT /evidence="ECO:0000250"
FT BINDING 519
FT /ligand="heme"
FT /ligand_id="ChEBI:CHEBI:30413"
FT /ligand_part="Fe"
FT /ligand_part_id="ChEBI:CHEBI:18248"
FT /note="axial binding residue"
FT /evidence="ECO:0000250"
FT CONFLICT 320..322
FT /note="VAG -> WRD (in Ref. 1; AAF76522)"
FT /evidence="ECO:0000305"
FT CONFLICT 336
FT /note="D -> N (in Ref. 1; AAF76522)"
FT /evidence="ECO:0000305"
FT CONFLICT 344
FT /note="E -> K (in Ref. 1; AAF76522)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 574 AA; 65380 MW; 3131878D3023DCF7 CRC64;
MEVLKKDAAL GSPSSVFYFL LLPTLVLWYI YWRLSRAHLY RLAGRLPGPR GLPIVGHLFD
VIGPASSVFR TVIRKSAPFE HIAKMWIGPK LVVFIYDPRD VELLLSSHVY IDKASEYKFF
KPWLGDGLLI STGQKWRSHR KLIAPTFHLN VLKSFIELFN ENSRNVVRKL RAEDGRTFDC
HDYMSEATVE ILLETAMGVS KKTQDKSGFE YAMAVMRMCD ILHARHRSIF LRNEFVFTLT
RYYKEQGRLL NIIHGLTTKV IRSKKAAFEQ GTRGSLAQCE LKAAALERER EQNGGVDQTP
STAGSDEKDR EKDKEKASPV AGLSYGQSAG LKDDLDVEDN DIGEKKRLAF LDLMLESAQN
GALITDTEIK EQVDTIMFEG HDTTAAGSSF FLSLMGIHQD IQDRVLAELD SIFGDSQRPA
TFQDTLEMKY LERCLMETLR MYPPVPLIAR ELQEDLKLNS GNYVIPRGAT VTVATVLLHR
NPKVYANPNV FDPDNFLPER QANRHYYAFV PFSAGPRSCV GRKYAMLKLK ILLSTILRNY
RVYSDLTESD FKLQADIILK REEGFRVRLQ PRTS
