TTD14_DROME
ID TTD14_DROME Reviewed; 475 AA.
AC Q7K556; A8DYI1;
DT 13-APR-2016, integrated into UniProtKB/Swiss-Prot.
DT 03-OCT-2006, sequence version 1.
DT 03-AUG-2022, entry version 125.
DE RecName: Full=TRPL translocation defect protein 14 {ECO:0000303|PubMed:26509977};
GN Name=Ttd14 {ECO:0000303|PubMed:26509977, ECO:0000312|FlyBase:FBgn0284257};
GN ORFNames=CG30118 {ECO:0000312|FlyBase:FBgn0284257};
OS Drosophila melanogaster (Fruit fly).
OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea;
OC Drosophilidae; Drosophila; Sophophora.
OX NCBI_TaxID=7227;
RN [1] {ECO:0000312|Proteomes:UP000000803}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Berkeley {ECO:0000312|Proteomes:UP000000803};
RX PubMed=10731132; DOI=10.1126/science.287.5461.2185;
RA Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D.,
RA Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F.,
RA George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N.,
RA Sutton G.G., Wortman J.R., Yandell M.D., Zhang Q., Chen L.X., Brandon R.C.,
RA Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C.,
RA Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A.,
RA An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A.,
RA Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V.,
RA Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J.,
RA Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E.,
RA Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B.,
RA Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I.,
RA Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C.,
RA Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S.,
RA Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M.,
RA Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M.,
RA Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D.,
RA Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F.,
RA Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D.,
RA Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A.,
RA Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C.,
RA McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C.,
RA Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L.,
RA Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R.,
RA Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V.,
RA Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F.,
RA Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J.,
RA Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R.,
RA Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y.,
RA Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T.,
RA Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S.,
RA Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W.,
RA Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M.,
RA Venter J.C.;
RT "The genome sequence of Drosophila melanogaster.";
RL Science 287:2185-2195(2000).
RN [2] {ECO:0000312|Proteomes:UP000000803}
RP GENOME REANNOTATION.
RC STRAIN=Berkeley {ECO:0000312|Proteomes:UP000000803};
RX PubMed=12537572; DOI=10.1186/gb-2002-3-12-research0083;
RA Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S.,
RA Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E.,
RA Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P.,
RA Bettencourt B.R., Celniker S.E., de Grey A.D.N.J., Drysdale R.A.,
RA Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M.,
RA Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E.;
RT "Annotation of the Drosophila melanogaster euchromatic genome: a systematic
RT review.";
RL Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002).
RN [3] {ECO:0000312|EMBL:AAK92870.1}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM A).
RC STRAIN=Berkeley {ECO:0000312|EMBL:AAK92870.1};
RC TISSUE=Head {ECO:0000312|EMBL:AAK92870.1};
RX PubMed=12537569; DOI=10.1186/gb-2002-3-12-research0080;
RA Stapleton M., Carlson J.W., Brokstein P., Yu C., Champe M., George R.A.,
RA Guarin H., Kronmiller B., Pacleb J.M., Park S., Wan K.H., Rubin G.M.,
RA Celniker S.E.;
RT "A Drosophila full-length cDNA resource.";
RL Genome Biol. 3:RESEARCH0080.1-RESEARCH0080.8(2002).
RN [4] {ECO:0000305}
RP FUNCTION, ALTERNATIVE SPLICING, TISSUE SPECIFICITY, AND MUTAGENESIS OF
RP PRO-75.
RX PubMed=26509977; DOI=10.1371/journal.pgen.1005578;
RA Cerny A.C., Altendorfer A., Schopf K., Baltner K., Maag N., Sehn E.,
RA Wolfrum U., Huber A.;
RT "The GTP- and phospholipid-binding protein TTD14 regulates trafficking of
RT the TRPL ion channel in Drosophila photoreceptor cells.";
RL PLoS Genet. 11:E1005578-E1005578(2015).
CC -!- FUNCTION: GTP-binding protein which is required for the light-dependent
CC internalization of the TRPL ion channel from the rhabdomere on the
CC apical surface of photoreceptor cells to the cell body and for the
CC recycling of TRPL back to the rhabdomere in the dark. Binds to 3-
CC phosphoinositide (PtdIns(3)P) and phosphatic acid and so may interact
CC with membranes, particularly the early endosome membrane where
CC PtdIns(3)P is predominantly localized. Plays a role in preventing
CC photoreceptor degeneration. {ECO:0000269|PubMed:26509977}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm, cytosol {ECO:0000269|PubMed:26509977}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=A {ECO:0000312|FlyBase:FBgn0284257};
CC IsoId=Q7K556-1; Sequence=Displayed;
CC Name=B {ECO:0000312|FlyBase:FBgn0284257};
CC IsoId=Q7K556-2; Sequence=VSP_058208;
CC -!- TISSUE SPECIFICITY: Isoform A: Expressed in the head. Isoform B:
CC Expressed in the head. {ECO:0000269|PubMed:26509977}.
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DR EMBL; AE013599; AAF57688.2; -; Genomic_DNA.
DR EMBL; AE013599; ABV53842.1; -; Genomic_DNA.
DR EMBL; AY051446; AAK92870.1; -; mRNA.
DR RefSeq; NP_001097365.1; NM_001103895.2. [Q7K556-2]
DR RefSeq; NP_611333.1; NM_137489.4. [Q7K556-1]
DR AlphaFoldDB; Q7K556; -.
DR IntAct; Q7K556; 2.
DR STRING; 7227.FBpp0112273; -.
DR PRIDE; Q7K556; -.
DR DNASU; 37119; -.
DR EnsemblMetazoa; FBtr0086724; FBpp0085903; FBgn0284257. [Q7K556-1]
DR EnsemblMetazoa; FBtr0113360; FBpp0112272; FBgn0284257. [Q7K556-2]
DR GeneID; 37119; -.
DR KEGG; dme:Dmel_CG30118; -.
DR UCSC; CG30118-RA; d. melanogaster. [Q7K556-1]
DR UCSC; CG30118-RB; d. melanogaster.
DR CTD; 37119; -.
DR FlyBase; FBgn0284257; Ttd14.
DR VEuPathDB; VectorBase:FBgn0284257; -.
DR eggNOG; ENOG502QVQD; Eukaryota.
DR GeneTree; ENSGT00520000060563; -.
DR HOGENOM; CLU_037796_0_1_1; -.
DR GenomeRNAi; 37119; -.
DR PRO; PR:Q7K556; -.
DR Proteomes; UP000000803; Chromosome 2R.
DR Bgee; FBgn0284257; Expressed in anlage in statu nascendi and 74 other tissues.
DR ExpressionAtlas; Q7K556; baseline and differential.
DR Genevisible; A8DYI1; DM.
DR GO; GO:0005829; C:cytosol; IEA:UniProtKB-SubCell.
DR GO; GO:0005525; F:GTP binding; IDA:UniProtKB.
DR GO; GO:0070300; F:phosphatidic acid binding; IDA:UniProtKB.
DR GO; GO:0035091; F:phosphatidylinositol binding; IDA:UniProtKB.
DR GO; GO:0045494; P:photoreceptor cell maintenance; IMP:UniProtKB.
DR GO; GO:0015031; P:protein transport; IEA:UniProtKB-KW.
DR Gene3D; 3.40.50.300; -; 1.
DR InterPro; IPR033469; CYTH-like_dom_sf.
DR InterPro; IPR038727; NadR/Ttd14_AAA_dom.
DR InterPro; IPR027417; P-loop_NTPase.
DR Pfam; PF13521; AAA_28; 1.
DR SUPFAM; SSF52540; SSF52540; 1.
DR SUPFAM; SSF55154; SSF55154; 1.
PE 1: Evidence at protein level;
KW Alternative splicing; Cytoplasm; GTP-binding; Lipid-binding;
KW Nucleotide-binding; Protein transport; Reference proteome; Transport.
FT CHAIN 1..475
FT /note="TRPL translocation defect protein 14"
FT /evidence="ECO:0000305"
FT /id="PRO_0000436009"
FT REGION 1..59
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 452..475
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT COMPBIAS 1..22
FT /note="Polar residues"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT VAR_SEQ 454..475
FT /note="GKQEEQSPVPAKQYHPYDENSD -> VKGAANGVSSTPLLLNGQ (in
FT isoform B)"
FT /id="VSP_058208"
FT MUTAGEN 75
FT /note="P->L: Lack of GTP-binding, defective light-induced
FT internalization of TRPL, late-onset light-dependent
FT photoreceptor degeneration and larval lethality."
FT /evidence="ECO:0000269|PubMed:26509977"
SQ SEQUENCE 475 AA; 54337 MW; 261D57E316B51697 CRC64;
MATLLSNGQQ SVMASTSNGQ EQQRHQDEPE QQQQVEIRKS SSPKPGAMPS LKLNRMGSVS
PKDKRVYKIV LTGGPCGGKT TGQSRLCTFF ENLGWKVFRV PETATVLLSG GVKFSDLTEK
EAYKFQENLI RTMVQIENTY FELGNSSNRN CLIICDRGVM DASAYISKDK WEKMMAGNNW
NPVEMRDNRY NQILHLVSAA NGAEDFYSTE DHACRSEGVD LARELDYKSA AAWVGHPYFD
VIDNSTNFET KMNRMIESVC QKLGIDIGDR LQATSRKLKY LVALLPPDSE FPPFQDFDVV
HHYLQSAGPK VQARLRKRGQ KNHWSYIHTQ RRPNVHGQAR IEVKTQLTHR DYMNLLAQRD
DAHFTIYKKR RCFLINNQYF QLDIYKEPGH PRCKGLVLLE TYSSLTGDAL KNCMPKFLNI
VKEVTGDPDY SMFNLSLKED WSTTKKFCRS ATHGKQEEQS PVPAKQYHPY DENSD
