TTDA_ECOLI
ID TTDA_ECOLI Reviewed; 303 AA.
AC P05847; P33130; Q2M9E4;
DT 01-NOV-1988, integrated into UniProtKB/Swiss-Prot.
DT 29-AUG-2003, sequence version 3.
DT 03-AUG-2022, entry version 157.
DE RecName: Full=L(+)-tartrate dehydratase subunit alpha;
DE Short=L-TTD alpha;
DE EC=4.2.1.32;
GN Name=ttdA; Synonyms=ygjA; OrderedLocusNames=b3061, JW3033;
OS Escherichia coli (strain K12).
OC Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC Enterobacteriaceae; Escherichia.
OX NCBI_TaxID=83333;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA], CATALYTIC ACTIVITY, SUBUNIT, AND
RP COFACTOR.
RC STRAIN=K12 / W3110 / ATCC 27325 / DSM 5911;
RX PubMed=8371115; DOI=10.1099/00221287-139-7-1523;
RA Reaney S.K., Begg C., Bungard S.I., Guest J.R.;
RT "Identification of the L-tartrate dehydratase genes (ttdA and ttdB) of
RT Escherichia coli and evolutionary relationship with the class I fumarase
RT genes.";
RL J. Gen. Microbiol. 139:1523-1530(1993).
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX PubMed=3297921; DOI=10.1016/0378-1119(87)90303-9;
RA Nesin M., Lupski J.R., Svec P., Godson G.N.;
RT "Possible new genes as revealed by molecular analysis of a 5-kb Escherichia
RT coli chromosomal region 5' to the rpsU-dnaG-rpoD macromolecular-synthesis
RT operon.";
RL Gene 51:149-161(1987).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / MG1655 / ATCC 47076;
RX PubMed=9278503; DOI=10.1126/science.277.5331.1453;
RA Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V.,
RA Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F.,
RA Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B.,
RA Shao Y.;
RT "The complete genome sequence of Escherichia coli K-12.";
RL Science 277:1453-1462(1997).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / W3110 / ATCC 27325 / DSM 5911;
RX PubMed=16738553; DOI=10.1038/msb4100049;
RA Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S.,
RA Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.;
RT "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655
RT and W3110.";
RL Mol. Syst. Biol. 2:E1-E5(2006).
RN [5]
RP INDUCTION.
RX PubMed=16804186; DOI=10.1099/mic.0.28753-0;
RA Oshima T., Biville F.;
RT "Functional identification of ygiP as a positive regulator of the ttdA-
RT ttdB-ygjE operon.";
RL Microbiology 152:2129-2135(2006).
CC -!- CATALYTIC ACTIVITY:
CC Reaction=(2R,3R)-tartrate = H2O + oxaloacetate; Xref=Rhea:RHEA:15413,
CC ChEBI:CHEBI:15377, ChEBI:CHEBI:16452, ChEBI:CHEBI:30924; EC=4.2.1.32;
CC Evidence={ECO:0000269|PubMed:8371115};
CC -!- COFACTOR:
CC Name=iron-sulfur cluster; Xref=ChEBI:CHEBI:30408;
CC Evidence={ECO:0000269|PubMed:8371115};
CC -!- SUBUNIT: Tetramer of two alpha and two beta subunits.
CC {ECO:0000305|PubMed:8371115}.
CC -!- INTERACTION:
CC P05847; P0ACL0: glpR; NbExp=2; IntAct=EBI-1113137, EBI-1123685;
CC -!- INDUCTION: Induced by tartrate, via TtdR.
CC {ECO:0000269|PubMed:16804186}.
CC -!- SIMILARITY: Belongs to the class-I fumarase family. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAA72573.1; Type=Frameshift; Evidence={ECO:0000305};
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DR EMBL; L14781; AAA03061.1; -; Unassigned_DNA.
DR EMBL; M16194; AAA72573.1; ALT_FRAME; Genomic_DNA.
DR EMBL; U28379; AAA89141.1; -; Genomic_DNA.
DR EMBL; U00096; AAC76097.1; -; Genomic_DNA.
DR EMBL; AP009048; BAE77112.1; -; Genomic_DNA.
DR PIR; C65094; QQECRT.
DR RefSeq; NP_417533.1; NC_000913.3.
DR RefSeq; WP_000986797.1; NZ_LN832404.1.
DR AlphaFoldDB; P05847; -.
DR SMR; P05847; -.
DR BioGRID; 4263296; 17.
DR ComplexPortal; CPX-5912; L-tartrate dehydratase complex.
DR IntAct; P05847; 6.
DR STRING; 511145.b3061; -.
DR PaxDb; P05847; -.
DR PRIDE; P05847; -.
DR EnsemblBacteria; AAC76097; AAC76097; b3061.
DR EnsemblBacteria; BAE77112; BAE77112; BAE77112.
DR GeneID; 66673040; -.
DR GeneID; 947565; -.
DR KEGG; ecj:JW3033; -.
DR KEGG; eco:b3061; -.
DR PATRIC; fig|1411691.4.peg.3670; -.
DR EchoBASE; EB1156; -.
DR eggNOG; COG1951; Bacteria.
DR HOGENOM; CLU_041245_1_0_6; -.
DR InParanoid; P05847; -.
DR OMA; IETYPTH; -.
DR PhylomeDB; P05847; -.
DR BioCyc; EcoCyc:TTDA-MON; -.
DR BioCyc; MetaCyc:TTDA-MON; -.
DR PRO; PR:P05847; -.
DR Proteomes; UP000000318; Chromosome.
DR Proteomes; UP000000625; Chromosome.
DR GO; GO:1902494; C:catalytic complex; IC:ComplexPortal.
DR GO; GO:0051539; F:4 iron, 4 sulfur cluster binding; IEA:UniProtKB-KW.
DR GO; GO:0008730; F:L(+)-tartrate dehydratase activity; IEA:UniProtKB-EC.
DR GO; GO:0046872; F:metal ion binding; IEA:UniProtKB-KW.
DR GO; GO:0044010; P:single-species biofilm formation; IMP:EcoCyc.
DR GO; GO:1901276; P:tartrate catabolic process; IC:ComplexPortal.
DR GO; GO:1901275; P:tartrate metabolic process; IMP:EcoCyc.
DR InterPro; IPR004646; Fe-S_hydro-lyase_TtdA-typ_cat.
DR Pfam; PF05681; Fumerase; 1.
DR TIGRFAMs; TIGR00722; ttdA_fumA_fumB; 1.
PE 1: Evidence at protein level;
KW 4Fe-4S; Iron; Iron-sulfur; Lyase; Metal-binding; Reference proteome.
FT CHAIN 1..303
FT /note="L(+)-tartrate dehydratase subunit alpha"
FT /id="PRO_0000195663"
FT BINDING 71
FT /ligand="iron-sulfur cluster"
FT /ligand_id="ChEBI:CHEBI:30408"
FT /evidence="ECO:0000250|UniProtKB:E9AE57"
FT BINDING 190
FT /ligand="iron-sulfur cluster"
FT /ligand_id="ChEBI:CHEBI:30408"
FT /evidence="ECO:0000250|UniProtKB:E9AE57"
FT BINDING 277
FT /ligand="iron-sulfur cluster"
FT /ligand_id="ChEBI:CHEBI:30408"
FT /evidence="ECO:0000250|UniProtKB:E9AE57"
FT CONFLICT 11
FT /note="V -> L (in Ref. 1 and 2)"
FT /evidence="ECO:0000305"
FT CONFLICT 231..232
FT /note="EL -> DV (in Ref. 1 and 2)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 303 AA; 32734 MW; CBE29FEB6C47DB81 CRC64;
MMSESNKQQA VNKLTEIVAN FTAMISTRMP DDVVDKLKQL KDAETSSMGK IIYHTMFDNM
QKAIDLNRPA CQDTGEIMFF VKVGSRFPLL GELQSILKQA VEEATVKAPL RHNAVEIFDE
VNTGKNTGSG VPWVTWDIIP DNDDAEIEVY MAGGGCTLPG RSKVLMPSEG YEGVVKFVFE
NISTLAVNAC PPVLVGVGIA TSVETAAVLS RKAILRPIGS RHPNPKAAEL ELRLEEGLNR
LGIGPQGLTG NSSVMGVHIE SAARHPSTIG VAVSTGCWAH RRGTLLVHAD LTFENLSHTR
SAL
