TTDT_ECOLI
ID TTDT_ECOLI Reviewed; 487 AA.
AC P39414; Q2M9E2; Q46870;
DT 01-FEB-1995, integrated into UniProtKB/Swiss-Prot.
DT 01-NOV-1997, sequence version 2.
DT 03-AUG-2022, entry version 151.
DE RecName: Full=L-tartrate/succinate antiporter;
DE AltName: Full=Tartrate carrier;
DE AltName: Full=Tartrate transporter;
GN Name=ttdT; Synonyms=ygjC, ygjE; OrderedLocusNames=b3063, JW3035;
OS Escherichia coli (strain K12).
OC Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC Enterobacteriaceae; Escherichia.
OX NCBI_TaxID=83333;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX PubMed=3297921; DOI=10.1016/0378-1119(87)90303-9;
RA Nesin M., Lupski J.R., Svec P., Godson G.N.;
RT "Possible new genes as revealed by molecular analysis of a 5-kb Escherichia
RT coli chromosomal region 5' to the rpsU-dnaG-rpoD macromolecular-synthesis
RT operon.";
RL Gene 51:149-161(1987).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / MG1655 / ATCC 47076;
RX PubMed=9278503; DOI=10.1126/science.277.5331.1453;
RA Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V.,
RA Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F.,
RA Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B.,
RA Shao Y.;
RT "The complete genome sequence of Escherichia coli K-12.";
RL Science 277:1453-1462(1997).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / W3110 / ATCC 27325 / DSM 5911;
RX PubMed=16738553; DOI=10.1038/msb4100049;
RA Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S.,
RA Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.;
RT "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655
RT and W3110.";
RL Mol. Syst. Biol. 2:E1-E5(2006).
RN [4]
RP IDENTIFICATION.
RX PubMed=7984428; DOI=10.1093/nar/22.22.4756;
RA Borodovsky M., Rudd K.E., Koonin E.V.;
RT "Intrinsic and extrinsic approaches for detecting genes in a bacterial
RT genome.";
RL Nucleic Acids Res. 22:4756-4767(1994).
RN [5]
RP POSSIBLE FUNCTION.
RX PubMed=9696764; DOI=10.1128/jb.180.16.4160-4165.1998;
RA Pos K.M., Dimroth P., Bott M.;
RT "The Escherichia coli citrate carrier CitT: a member of a novel eubacterial
RT transporter family related to the 2-oxoglutarate/malate translocator from
RT spinach chloroplasts.";
RL J. Bacteriol. 180:4160-4165(1998).
RN [6]
RP TOPOLOGY [LARGE SCALE ANALYSIS].
RC STRAIN=K12 / MG1655 / ATCC 47076;
RX PubMed=15919996; DOI=10.1126/science.1109730;
RA Daley D.O., Rapp M., Granseth E., Melen K., Drew D., von Heijne G.;
RT "Global topology analysis of the Escherichia coli inner membrane
RT proteome.";
RL Science 308:1321-1323(2005).
RN [7]
RP INDUCTION.
RX PubMed=16804186; DOI=10.1099/mic.0.28753-0;
RA Oshima T., Biville F.;
RT "Functional identification of ygiP as a positive regulator of the ttdA-
RT ttdB-ygjE operon.";
RL Microbiology 152:2129-2135(2006).
RN [8]
RP FUNCTION, AND BIOPHYSICOCHEMICAL PROPERTIES.
RC STRAIN=K12 / MG1655 / ATCC 47076;
RX PubMed=17172328; DOI=10.1128/jb.01402-06;
RA Kim O.B., Unden G.;
RT "The L-tartrate/succinate antiporter TtdT (YgjE) of L-tartrate fermentation
RT in Escherichia coli.";
RL J. Bacteriol. 189:1597-1603(2007).
CC -!- FUNCTION: Catalyzes the uptake of tartrate in exchange for
CC intracellular succinate. Essential for anaerobic L-tartrate
CC fermentation. {ECO:0000269|PubMed:17172328}.
CC -!- BIOPHYSICOCHEMICAL PROPERTIES:
CC Kinetic parameters:
CC KM=700 uM for tartrate {ECO:0000269|PubMed:17172328};
CC KM=400 uM for succinate {ECO:0000269|PubMed:17172328};
CC Vmax=110 umol/min/g enzyme for tartrate import
CC {ECO:0000269|PubMed:17172328};
CC Vmax=16 umol/min/g enzyme for succinate export
CC {ECO:0000269|PubMed:17172328};
CC -!- SUBCELLULAR LOCATION: Cell inner membrane; Multi-pass membrane protein.
CC -!- INDUCTION: Induced by tartrate, via TtdR.
CC {ECO:0000269|PubMed:16804186}.
CC -!- SIMILARITY: Belongs to the SLC13A/DASS transporter (TC 2.A.47) family.
CC DIT1 subfamily. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=M16194; Type=Frameshift; Evidence={ECO:0000305};
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DR EMBL; M16194; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR EMBL; U28379; AAA89143.1; -; Genomic_DNA.
DR EMBL; U00096; AAC76099.1; -; Genomic_DNA.
DR EMBL; AP009048; BAE77114.1; -; Genomic_DNA.
DR PIR; E65094; QQECRS.
DR RefSeq; NP_417535.1; NC_000913.3.
DR RefSeq; WP_000804973.1; NZ_LN832404.1.
DR AlphaFoldDB; P39414; -.
DR SMR; P39414; -.
DR BioGRID; 4262386; 10.
DR STRING; 511145.b3063; -.
DR TCDB; 2.A.47.3.3; the divalent anion:na(+) symporter (dass) family.
DR PaxDb; P39414; -.
DR PRIDE; P39414; -.
DR EnsemblBacteria; AAC76099; AAC76099; b3063.
DR EnsemblBacteria; BAE77114; BAE77114; BAE77114.
DR GeneID; 947576; -.
DR KEGG; ecj:JW3035; -.
DR KEGG; eco:b3063; -.
DR PATRIC; fig|511145.12.peg.3157; -.
DR EchoBASE; EB2294; -.
DR eggNOG; COG0471; Bacteria.
DR HOGENOM; CLU_005170_7_0_6; -.
DR InParanoid; P39414; -.
DR OMA; YAWHFFA; -.
DR PhylomeDB; P39414; -.
DR BioCyc; EcoCyc:YGJE-MON; -.
DR BioCyc; MetaCyc:YGJE-MON; -.
DR PRO; PR:P39414; -.
DR Proteomes; UP000000318; Chromosome.
DR Proteomes; UP000000625; Chromosome.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0005886; C:plasma membrane; IDA:EcoCyc.
DR GO; GO:0015297; F:antiporter activity; IMP:EcoliWiki.
DR InterPro; IPR030676; CitT-rel.
DR InterPro; IPR001898; SLC13A/DASS.
DR PANTHER; PTHR42826; PTHR42826; 1.
DR Pfam; PF00939; Na_sulph_symp; 1.
DR PIRSF; PIRSF002457; DASS; 1.
DR TIGRFAMs; TIGR00785; dass; 1.
PE 1: Evidence at protein level;
KW Antiport; Cell inner membrane; Cell membrane; Membrane; Reference proteome;
KW Transmembrane; Transmembrane helix; Transport.
FT CHAIN 1..487
FT /note="L-tartrate/succinate antiporter"
FT /id="PRO_0000172525"
FT TOPO_DOM 1..9
FT /note="Periplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 10..30
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 31..32
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 33..53
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 54..74
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 75..92
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 93..113
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 114..136
FT /note="Periplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 137..157
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 158..188
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 189..209
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 210..235
FT /note="Periplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 236..256
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 257..291
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 292..312
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 313..333
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 334..339
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 340..360
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 361..369
FT /note="Periplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 370..390
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 391..392
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 393..413
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 414..417
FT /note="Periplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 418..438
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 439..464
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 465..485
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 486..487
FT /note="Periplasmic"
FT /evidence="ECO:0000255"
FT CONFLICT 404
FT /note="L -> P (in Ref. 1)"
FT /evidence="ECO:0000305"
FT CONFLICT 457
FT /note="A -> T (in Ref. 1)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 487 AA; 52906 MW; 4D6E8439BEE281D2 CRC64;
MKPSTEWWRY LAPLAVIAII ALLPVPAGLE NHTWLYFAVF TGVIVGLILE PVPGAVVAMV
GISIIAILSP WLLFSPEQLA QPGFKFTAKS LSWAVSGFSN SVIWLIFAAF MFGTGYEKTG
LGRRIALILV KKMGHRTLFL GYAVMFSELI LAPVTPSNSA RGAGIIYPII RNLPPLYQSQ
PNDSSSRSIG SYIMWMGIVA DCVTSAIFLT AMAPNLLLIG LMKSASHATL SWGDWFLGML
PLSILLVLLV PWLAYVLYPP VLKSGDQVPR WAETELQAMG PLCSREKRML GLMVGALVLW
IFGGDYIDAA MVGYSVVALM LLLRIISWDD IVSNKAAWNV FFWLASLITL ATGLNNTGFI
SWFGKLLAGS LSGYSPTMVM VALIVVFYLL RYFFASATAY TSALAPMMIA AALAMPEIPL
PVFCLMVGAA IGLGSILTPY ATGPSPIYYG SGYLPTADYW RLGAIFGLIF LVLLVITGLL
WMPVVLL
