ID   TTGA_PSEPK              Reviewed;         384 AA.
AC   Q88N30;
DT   19-JUL-2005, integrated into UniProtKB/Swiss-Prot.
DT   01-JUN-2003, sequence version 1.
DT   03-AUG-2022, entry version 104.
DE   RecName: Full=Probable efflux pump periplasmic linker TtgA;
DE   Flags: Precursor;
GN   Name=ttgA; OrderedLocusNames=PP_1386;
OS   Pseudomonas putida (strain ATCC 47054 / DSM 6125 / CFBP 8728 / NCIMB 11950
OS   / KT2440).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Pseudomonadales;
OC   Pseudomonadaceae; Pseudomonas.
OX   NCBI_TaxID=160488;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 47054 / DSM 6125 / CFBP 8728 / NCIMB 11950 / KT2440;
RX   PubMed=12534463; DOI=10.1046/j.1462-2920.2002.00366.x;
RA   Nelson K.E., Weinel C., Paulsen I.T., Dodson R.J., Hilbert H.,
RA   Martins dos Santos V.A.P., Fouts D.E., Gill S.R., Pop M., Holmes M.,
RA   Brinkac L.M., Beanan M.J., DeBoy R.T., Daugherty S.C., Kolonay J.F.,
RA   Madupu R., Nelson W.C., White O., Peterson J.D., Khouri H.M., Hance I.,
RA   Chris Lee P., Holtzapple E.K., Scanlan D., Tran K., Moazzez A.,
RA   Utterback T.R., Rizzo M., Lee K., Kosack D., Moestl D., Wedler H.,
RA   Lauber J., Stjepandic D., Hoheisel J., Straetz M., Heim S., Kiewitz C.,
RA   Eisen J.A., Timmis K.N., Duesterhoeft A., Tuemmler B., Fraser C.M.;
RT   "Complete genome sequence and comparative analysis of the metabolically
RT   versatile Pseudomonas putida KT2440.";
RL   Environ. Microbiol. 4:799-808(2002).
RN   [2]
RP   CHARACTERIZATION.
RX   PubMed=12743835; DOI=10.1007/s00792-003-0331-x;
RA   Segura A., Rojas A., Hurtado A., Huertas M.J., Ramos J.L.;
RT   "Comparative genomic analysis of solvent extrusion pumps in Pseudomonas
RT   strains exhibiting different degrees of solvent tolerance.";
RL   Extremophiles 7:371-376(2003).
CC   -!- FUNCTION: Probable periplasmic linker protein component of the TtgABC
CC       efflux pump with unknown specificity.
CC   -!- SUBCELLULAR LOCATION: Cell inner membrane {ECO:0000305}; Lipid-anchor
CC       {ECO:0000255|PROSITE-ProRule:PRU00303}.
CC   -!- SIMILARITY: Belongs to the membrane fusion protein (MFP) (TC 8.A.1)
CC       family. {ECO:0000305}.
CC   -!- CAUTION: There are 4 nearly identical operons in various strains of
CC       P.putida. The ttgABC operon of strain DOT-T1E and the mepABC operon of
CC       strain KT2442-TOL function in solvent and antibiotic efflux; however in
CC       strain S12 the arpABC operon functions only in antibiotic efflux. This
CC       may be due to different protein expression levels. In KT2400 this
CC       operon does not seem to function in toluene efflux. {ECO:0000305}.
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DR   EMBL; AE015451; AAN67009.1; -; Genomic_DNA.
DR   RefSeq; NP_743545.1; NC_002947.4.
DR   RefSeq; WP_010952494.1; NC_002947.4.
DR   AlphaFoldDB; Q88N30; -.
DR   SMR; Q88N30; -.
DR   STRING; 160488.PP_1386; -.
DR   EnsemblBacteria; AAN67009; AAN67009; PP_1386.
DR   KEGG; ppu:PP_1386; -.
DR   PATRIC; fig|160488.4.peg.1470; -.
DR   eggNOG; COG0845; Bacteria.
DR   HOGENOM; CLU_018816_2_1_6; -.
DR   OMA; INVRYTK; -.
DR   PhylomeDB; Q88N30; -.
DR   BioCyc; PPUT160488:G1G01-1476-MON; -.
DR   Proteomes; UP000000556; Chromosome.
DR   GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0022857; F:transmembrane transporter activity; IEA:InterPro.
DR   InterPro; IPR043602; CusB_dom_1.
DR   InterPro; IPR032317; HlyD_D23.
DR   InterPro; IPR006143; RND_pump_MFP.
DR   Pfam; PF00529; CusB_dom_1; 1.
DR   Pfam; PF16576; HlyD_D23; 1.
DR   TIGRFAMs; TIGR01730; RND_mfp; 1.
DR   PROSITE; PS51257; PROKAR_LIPOPROTEIN; 1.
PE   1: Evidence at protein level;
KW   Cell inner membrane; Cell membrane; Coiled coil; Lipoprotein; Membrane;
KW   Palmitate; Reference proteome; Signal; Transport.
FT   SIGNAL          1..22
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00303"
FT   CHAIN           23..384
FT                   /note="Probable efflux pump periplasmic linker TtgA"
FT                   /id="PRO_0000018717"
FT   REGION          362..384
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COILED          115..155
FT                   /evidence="ECO:0000255"
FT   LIPID           23
FT                   /note="N-palmitoyl cysteine"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00303"
FT   LIPID           23
FT                   /note="S-diacylglycerol cysteine"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00303"
SQ   SEQUENCE   384 AA;  41250 MW;  16BB3FA87E3C16CA CRC64;
     MQFKPAVTAL VSAVALATLL SGCKKEEAAP AAQAPQVGVV TIQPQAFTLT SELPGRTSAY
     RVAEVRPQVN GIILKRLFKE GSEVKEGQQL YQIDPAVYEA TLANAKANLL ATRSLAERYK
     QLIDEQAVSK QEYDDANAKR LQAEASLKSA QIDLRYTKVL APISGRIGRS SFTEGALVSN
     GQTDAMATIQ QLDPIYVDVT QSTAELLKLR RDLESGQLQK AGDNAASVQL VLEDGSLFKQ
     EGRLEFSEVA VDETTGSVTL RALFPNPDHT LLPGMFVHAR LKAGVNANAI LAPQQGVTRD
     LKGAPTALVV NQENKVELRQ LKASRTLGSD WLIEEGLNPG DRLITEGLQY VRPGVEVKVS
     DATNVKKPAG PDQANAAKAD AKAE