ID   TTGA_PSEPT              Reviewed;         384 AA.
AC   Q9WWZ9; I7BPU0;
DT   19-JUL-2005, integrated into UniProtKB/Swiss-Prot.
DT   01-NOV-1999, sequence version 1.
DT   25-MAY-2022, entry version 87.
DE   RecName: Full=Toluene efflux pump periplasmic linker protein TtgA;
DE   Flags: Precursor;
GN   Name=ttgA; OrderedLocusNames=T1E_0243;
OS   Pseudomonas putida (strain DOT-T1E).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Pseudomonadales;
OC   Pseudomonadaceae; Pseudomonas.
OX   NCBI_TaxID=1196325;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC   STRAIN=DOT-T1E;
RX   PubMed=9642183; DOI=10.1128/jb.180.13.3323-3329.1998;
RA   Ramos J.L., Duque E., Godoy P., Segura A.;
RT   "Efflux pumps involved in toluene tolerance in Pseudomonas putida DOT-
RT   T1E.";
RL   J. Bacteriol. 180:3323-3329(1998).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=DOT-T1E;
RX   PubMed=23815283; DOI=10.1111/1751-7915.12061;
RA   Udaondo Z., Molina L., Daniels C., Gomez M.J., Molina-Henares M.A.,
RA   Matilla M.A., Roca A., Fernandez M., Duque E., Segura A., Ramos J.L.;
RT   "Metabolic potential of the organic-solvent tolerant Pseudomonas putida
RT   DOT-T1E deduced from its annotated genome.";
RL   Microb. Biotechnol. 6:598-611(2013).
RN   [3]
RP   EFFLUX PUMP SUBSTRATES.
RC   STRAIN=DOT-T1E;
RX   PubMed=11395460; DOI=10.1128/jb.183.13.3967-3973.2001;
RA   Rojas A., Duque E., Mosqueda G., Golden G., Hurtado A., Ramos J.L.,
RA   Segura A.;
RT   "Three efflux pumps are required to provide efficient tolerance to toluene
RT   in Pseudomonas putida DOT-T1E.";
RL   J. Bacteriol. 183:3967-3973(2001).
RN   [4]
RP   INDUCTION.
RC   STRAIN=DOT-T1E;
RX   PubMed=14506010; DOI=10.1128/aac.47.10.3067-3072.2003;
RA   Teran W., Felipe A., Segura A., Rojas A., Ramos J.L., Gallegos M.T.;
RT   "Antibiotic-dependent induction of Pseudomonas putida DOT-T1E TtgABC efflux
RT   pump is mediated by the drug binding repressor TtgR.";
RL   Antimicrob. Agents Chemother. 47:3067-3072(2003).
CC   -!- FUNCTION: The periplasmic linker protein component of a constitutive
CC       organic solvent efflux system. Involved in export of toluene, styrene,
CC       m-xylene, propylbenzene and ethylbenzene. Also exports AMP and the
CC       antibiotics carbenicillin, nalidixic acid, chloramphenicol and
CC       tetracycline.
CC   -!- SUBCELLULAR LOCATION: Cell inner membrane {ECO:0000305}; Lipid-anchor
CC       {ECO:0000255|PROSITE-ProRule:PRU00303}.
CC   -!- INDUCTION: The ttgABC operon is repressed by toluene; this is mediated
CC       by TtgR. The ttgABC operon is induced in response to chloramphenicol
CC       and tetracycline. {ECO:0000269|PubMed:14506010}.
CC   -!- SIMILARITY: Belongs to the membrane fusion protein (MFP) (TC 8.A.1)
CC       family. {ECO:0000305}.
CC   -!- CAUTION: There are 4 nearly identical operons in various strains of
CC       P.putida. This one and the mepABC operon of strain KT2442-TOL function
CC       in solvent and antibiotic efflux; however the arpABC operon of strain
CC       S12 functions only in antibiotic efflux. This may be due to different
CC       protein expression levels. In strain KT2440 the equivalent operon does
CC       not seem to function in toluene efflux. {ECO:0000305}.
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DR   EMBL; AF031417; AAD39553.1; -; Genomic_DNA.
DR   EMBL; CP003734; AFO46102.1; -; Genomic_DNA.
DR   RefSeq; WP_014592249.1; NC_018220.1.
DR   AlphaFoldDB; Q9WWZ9; -.
DR   SMR; Q9WWZ9; -.
DR   TCDB; 2.A.6.2.9; the resistance-nodulation-cell division (rnd) superfamily.
DR   EnsemblBacteria; AFO46102; AFO46102; T1E_0243.
DR   KEGG; ppx:T1E_0243; -.
DR   PATRIC; fig|1196325.3.peg.244; -.
DR   HOGENOM; CLU_018816_2_1_6; -.
DR   Proteomes; UP000006503; Chromosome.
DR   GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0022857; F:transmembrane transporter activity; IEA:InterPro.
DR   GO; GO:0046677; P:response to antibiotic; IEA:UniProtKB-KW.
DR   InterPro; IPR043602; CusB_dom_1.
DR   InterPro; IPR032317; HlyD_D23.
DR   InterPro; IPR006143; RND_pump_MFP.
DR   Pfam; PF00529; CusB_dom_1; 1.
DR   Pfam; PF16576; HlyD_D23; 1.
DR   TIGRFAMs; TIGR01730; RND_mfp; 1.
DR   PROSITE; PS51257; PROKAR_LIPOPROTEIN; 1.
PE   2: Evidence at transcript level;
KW   Antibiotic resistance; Cell inner membrane; Cell membrane; Coiled coil;
KW   Lipoprotein; Membrane; Palmitate; Signal; Transport.
FT   SIGNAL          1..22
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00303"
FT   CHAIN           23..384
FT                   /note="Toluene efflux pump periplasmic linker protein TtgA"
FT                   /id="PRO_0000018718"
FT   REGION          362..384
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COILED          115..155
FT                   /evidence="ECO:0000255"
FT   LIPID           23
FT                   /note="N-palmitoyl cysteine"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00303"
FT   LIPID           23
FT                   /note="S-diacylglycerol cysteine"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00303"
SQ   SEQUENCE   384 AA;  41249 MW;  1E1F9BE412DCF6C4 CRC64;
     MQFKPAVTAL VSAVALATLL SGCKKEEAAP AAQAPQVGVV TIQPQAFTLT SELPGRTSAY
     RVAEVRPQVN GIILKRLFKE GSEVKEGQQL YQIDPAVYEA TLANAKANLL ATRSLAERYK
     QLIDEQAVSK QEYDDANAKR LQAEASLKSA QIDLRYTKVL APISGRIGRS SFTEGALVSN
     GQTDAMATIQ QLDPIYVDVT QSTAELLKLR RDLESGQLQK AGNNAASVQL VLEDGSLFKQ
     EGRLEFSEVA VDETTGSVTL RALFPNPDHT LLPGMFVHAR LKAGVNANAI LAPQQGVTRD
     LKGAPTALVV NQENKVELRQ LKASRTLGSD WLIEEGLNPG DRLITEGLQY VRPGVEVKVS
     DATNVKKPAG PDQANAAKAD AKAE