ID   TTGB_PSEPK              Reviewed;        1050 AA.
AC   Q88N31;
DT   19-JUL-2005, integrated into UniProtKB/Swiss-Prot.
DT   01-JUN-2003, sequence version 1.
DT   03-AUG-2022, entry version 96.
DE   RecName: Full=Probable efflux pump membrane transporter TtgB;
GN   Name=ttgB; OrderedLocusNames=PP_1385;
OS   Pseudomonas putida (strain ATCC 47054 / DSM 6125 / CFBP 8728 / NCIMB 11950
OS   / KT2440).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Pseudomonadales;
OC   Pseudomonadaceae; Pseudomonas.
OX   NCBI_TaxID=160488;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 47054 / DSM 6125 / CFBP 8728 / NCIMB 11950 / KT2440;
RX   PubMed=12534463; DOI=10.1046/j.1462-2920.2002.00366.x;
RA   Nelson K.E., Weinel C., Paulsen I.T., Dodson R.J., Hilbert H.,
RA   Martins dos Santos V.A.P., Fouts D.E., Gill S.R., Pop M., Holmes M.,
RA   Brinkac L.M., Beanan M.J., DeBoy R.T., Daugherty S.C., Kolonay J.F.,
RA   Madupu R., Nelson W.C., White O., Peterson J.D., Khouri H.M., Hance I.,
RA   Chris Lee P., Holtzapple E.K., Scanlan D., Tran K., Moazzez A.,
RA   Utterback T.R., Rizzo M., Lee K., Kosack D., Moestl D., Wedler H.,
RA   Lauber J., Stjepandic D., Hoheisel J., Straetz M., Heim S., Kiewitz C.,
RA   Eisen J.A., Timmis K.N., Duesterhoeft A., Tuemmler B., Fraser C.M.;
RT   "Complete genome sequence and comparative analysis of the metabolically
RT   versatile Pseudomonas putida KT2440.";
RL   Environ. Microbiol. 4:799-808(2002).
RN   [2]
RP   CHARACTERIZATION.
RX   PubMed=12743835; DOI=10.1007/s00792-003-0331-x;
RA   Segura A., Rojas A., Hurtado A., Huertas M.J., Ramos J.L.;
RT   "Comparative genomic analysis of solvent extrusion pumps in Pseudomonas
RT   strains exhibiting different degrees of solvent tolerance.";
RL   Extremophiles 7:371-376(2003).
CC   -!- FUNCTION: Probable membrane transporter component of the TtgABC efflux
CC       pump with unknown specificity.
CC   -!- SUBCELLULAR LOCATION: Cell inner membrane {ECO:0000305}; Multi-pass
CC       membrane protein {ECO:0000305}.
CC   -!- SIMILARITY: Belongs to the resistance-nodulation-cell division (RND)
CC       (TC 2.A.6) family. {ECO:0000305}.
CC   -!- CAUTION: There are 4 nearly identical operons in various strains of
CC       P.putida. The ttgABC operon of strain DOT-T1E and the mepABC operon of
CC       strain KT2442-TOL function in solvent and antibiotic efflux; however in
CC       strain S12 the arpABC operon functions only in antibiotic efflux. This
CC       may be due to different protein expression levels. In KT2400 this
CC       operon does not seem to function in toluene efflux. {ECO:0000305}.
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DR   EMBL; AE015451; AAN67008.1; -; Genomic_DNA.
DR   RefSeq; NP_743544.1; NC_002947.4.
DR   RefSeq; WP_010952493.1; NC_002947.4.
DR   AlphaFoldDB; Q88N31; -.
DR   SMR; Q88N31; -.
DR   STRING; 160488.PP_1385; -.
DR   EnsemblBacteria; AAN67008; AAN67008; PP_1385.
DR   KEGG; ppu:PP_1385; -.
DR   PATRIC; fig|160488.4.peg.1469; -.
DR   eggNOG; COG0841; Bacteria.
DR   HOGENOM; CLU_002755_0_1_6; -.
DR   OMA; LMYMSSS; -.
DR   PhylomeDB; Q88N31; -.
DR   BioCyc; PPUT160488:G1G01-1475-MON; -.
DR   Proteomes; UP000000556; Chromosome.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0015562; F:efflux transmembrane transporter activity; IEA:InterPro.
DR   GO; GO:0042908; P:xenobiotic transport; IEA:InterPro.
DR   Gene3D; 3.30.2090.10; -; 2.
DR   InterPro; IPR027463; AcrB_DN_DC_subdom.
DR   InterPro; IPR001036; Acrflvin-R.
DR   InterPro; IPR004764; HAE1.
DR   PANTHER; PTHR32063; PTHR32063; 1.
DR   Pfam; PF00873; ACR_tran; 1.
DR   PRINTS; PR00702; ACRIFLAVINRP.
DR   SUPFAM; SSF82714; SSF82714; 2.
DR   TIGRFAMs; TIGR00915; 2A0602; 1.
PE   1: Evidence at protein level;
KW   Cell inner membrane; Cell membrane; Membrane; Reference proteome;
KW   Transmembrane; Transmembrane helix; Transport.
FT   CHAIN           1..1050
FT                   /note="Probable efflux pump membrane transporter TtgB"
FT                   /id="PRO_0000161848"
FT   TRANSMEM        10..30
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        339..359
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        370..390
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        393..413
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        440..460
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        472..492
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        539..559
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        871..891
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        893..913
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        923..943
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        972..992
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        1004..1024
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
SQ   SEQUENCE   1050 AA;  112836 MW;  B2F0872BE50A0E53 CRC64;
     MSKFFIDRPI FAWVIALVIM LVGALSILKL PINQYPSIAP PAIAIAVTYP GASAQTVQDT
     VVQVIEQQLN GIDNLRYVSS ESNSDGSMTI TATFEQGTNP DTAQVQVQNK LNLATPLLPQ
     EVQQQGIRVT KAVKNFLLVI GLVSEDGSMT KDDLANYIVS NMQDPISRTA GVGDFQVFGA
     QYAMRIWLDP AKLNKFQLTP VDVKTAVAAQ NVQVSSGQLG GLPALPGTQL NATIIGKTRL
     QTAEQFESIL LKVNKDGSQV RLGDVAQVGL GGENYAVSAQ FNGKPASGLA VKLATGANAL
     DTAKALRETI KGLEPFFPPG VKAVFPYDTT PVVTESISGV IHTLIEAVVL VFLVMYLFLQ
     NFRATIITTM TVPVVLLGTF GILAAAGFSI NTLTMFAMVL AIGLLVDDAI VVVENVERVM
     SEEGLPPKEA TKRSMEQIQG ALVGIALVLS AVLLPMAFFG GSTGVIYRQF SITIVSAMGL
     SVLVALIFTP ALCATMLKPL KKGEHHTAKG GFFGWFNRNF DRSVNGYERS VGAILRNKVP
     FLLAYALIVV GMIWLFARIP TAFLPEEDQG VLFAQVQTPA GSSAERTQVV VDQMREYLLK
     DEADTVSSVF TVNGFNFAGR GQSSGMAFIM LKPWDERSKE NSVFALAQRA QQHFFTFRDA
     MVFAFAPPAV LELGNATGFD VFLQDRGGVG HEKLMEARNQ FLAKAAQSKI LSAVRPNGLN
     DEPQYQLTID DERASALGVT IADINNTLSI ALGASYVNDF IDRGRVKKVY IQGEPSARMS
     PEDLQKWYVR NGAGEMVPFS SFAKGEWTYG SPKLSRYNGV EAMEILGAPA PGYSTGEAMA
     EVERIAGELP SGIGFSWTGM SYEEKLSGSQ MPALFALSVL FVFLCLAALY ESWSIPIAVV
     LVVPLGIIGA LIATSLRGLS NDVYFLVGLL TTIGLAAKNA ILIVEFAKEL HEQGRSLYDA
     AIEACRMRLR PIIMTSLAFI LGVVPLTIAS GAGAGSQHAI GTGVIGGMIS ATVLAIFWVP
     LFFVAVSSLF GSKEPEKDVT PENPRYEAGQ