TTGB_PSEPT
ID TTGB_PSEPT Reviewed; 1050 AA.
AC O52248; I7BZ53;
DT 19-JUL-2005, integrated into UniProtKB/Swiss-Prot.
DT 01-NOV-1998, sequence version 2.
DT 25-MAY-2022, entry version 88.
DE RecName: Full=Toluene efflux pump membrane transporter TtgB;
GN Name=ttgB; OrderedLocusNames=T1E_0242;
OS Pseudomonas putida (strain DOT-T1E).
OC Bacteria; Proteobacteria; Gammaproteobacteria; Pseudomonadales;
OC Pseudomonadaceae; Pseudomonas.
OX NCBI_TaxID=1196325;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND FUNCTION.
RC STRAIN=DOT-T1E;
RX PubMed=9642183; DOI=10.1128/jb.180.13.3323-3329.1998;
RA Ramos J.L., Duque E., Godoy P., Segura A.;
RT "Efflux pumps involved in toluene tolerance in Pseudomonas putida DOT-
RT T1E.";
RL J. Bacteriol. 180:3323-3329(1998).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=DOT-T1E;
RX PubMed=23815283; DOI=10.1111/1751-7915.12061;
RA Udaondo Z., Molina L., Daniels C., Gomez M.J., Molina-Henares M.A.,
RA Matilla M.A., Roca A., Fernandez M., Duque E., Segura A., Ramos J.L.;
RT "Metabolic potential of the organic-solvent tolerant Pseudomonas putida
RT DOT-T1E deduced from its annotated genome.";
RL Microb. Biotechnol. 6:598-611(2013).
RN [3]
RP EFFLUX PUMP SUBSTRATES.
RC STRAIN=DOT-T1E;
RX PubMed=11395460; DOI=10.1128/jb.183.13.3967-3973.2001;
RA Rojas A., Duque E., Mosqueda G., Golden G., Hurtado A., Ramos J.L.,
RA Segura A.;
RT "Three efflux pumps are required to provide efficient tolerance to toluene
RT in Pseudomonas putida DOT-T1E.";
RL J. Bacteriol. 183:3967-3973(2001).
RN [4]
RP INDUCTION.
RC STRAIN=DOT-T1E;
RX PubMed=14506010; DOI=10.1128/aac.47.10.3067-3072.2003;
RA Teran W., Felipe A., Segura A., Rojas A., Ramos J.L., Gallegos M.T.;
RT "Antibiotic-dependent induction of Pseudomonas putida DOT-T1E TtgABC efflux
RT pump is mediated by the drug binding repressor TtgR.";
RL Antimicrob. Agents Chemother. 47:3067-3072(2003).
CC -!- FUNCTION: The inner membrane transporter component of a constitutive
CC organic solvent efflux system. Involved in export of toluene, styrene,
CC m-xylene, propylbenzene and ethylbenzene. Also exports AMP and the
CC antibiotics carbenicillin, nalidixic acid, chloramphenicol and
CC tetracycline. {ECO:0000269|PubMed:9642183}.
CC -!- SUBCELLULAR LOCATION: Cell inner membrane {ECO:0000305}; Multi-pass
CC membrane protein {ECO:0000305}.
CC -!- INDUCTION: The ttgABC operon is repressed by toluene; this is mediated
CC by TtgR. The ttgABC operon is induced in response to chloramphenicol
CC and tetracycline. {ECO:0000269|PubMed:14506010}.
CC -!- SIMILARITY: Belongs to the resistance-nodulation-cell division (RND)
CC (TC 2.A.6) family. {ECO:0000305}.
CC -!- CAUTION: There are 4 nearly identical operons in various strains of
CC P.putida. This one and the mepABC operon of strain KT2442-TOL function
CC in solvent and antibiotic efflux; however the arpABC operon of strain
CC S12 functions only in antibiotic efflux. This may be due to different
CC protein expression levels. In strain KT2440 the equivalent operon does
CC not seem to function in toluene efflux. {ECO:0000305}.
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DR EMBL; AF031417; AAC38671.1; -; Genomic_DNA.
DR EMBL; CP003734; AFO46101.1; -; Genomic_DNA.
DR RefSeq; WP_014754360.1; NC_018220.1.
DR AlphaFoldDB; O52248; -.
DR SMR; O52248; -.
DR TCDB; 2.A.6.2.9; the resistance-nodulation-cell division (rnd) superfamily.
DR EnsemblBacteria; AFO46101; AFO46101; T1E_0242.
DR KEGG; ppx:T1E_0242; -.
DR PATRIC; fig|1196325.3.peg.243; -.
DR HOGENOM; CLU_002755_0_1_6; -.
DR Proteomes; UP000006503; Chromosome.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0015562; F:efflux transmembrane transporter activity; IEA:InterPro.
DR GO; GO:0046677; P:response to antibiotic; IEA:UniProtKB-KW.
DR GO; GO:0042908; P:xenobiotic transport; IEA:InterPro.
DR Gene3D; 3.30.2090.10; -; 2.
DR InterPro; IPR027463; AcrB_DN_DC_subdom.
DR InterPro; IPR001036; Acrflvin-R.
DR InterPro; IPR004764; HAE1.
DR PANTHER; PTHR32063; PTHR32063; 1.
DR Pfam; PF00873; ACR_tran; 1.
DR PRINTS; PR00702; ACRIFLAVINRP.
DR SUPFAM; SSF82714; SSF82714; 2.
DR TIGRFAMs; TIGR00915; 2A0602; 1.
PE 2: Evidence at transcript level;
KW Antibiotic resistance; Cell inner membrane; Cell membrane; Membrane;
KW Transmembrane; Transmembrane helix; Transport.
FT CHAIN 1..1050
FT /note="Toluene efflux pump membrane transporter TtgB"
FT /id="PRO_0000161849"
FT TRANSMEM 10..30
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 339..359
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 370..390
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 393..413
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 440..460
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 472..492
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 539..559
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 871..891
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 893..913
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 923..943
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 972..992
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 1004..1024
FT /note="Helical"
FT /evidence="ECO:0000255"
SQ SEQUENCE 1050 AA; 112808 MW; CEC4552686C5E49C CRC64;
MSKFFIDRPI FAWVIALVIM LVGALSILKL PINQYPSIAP PAIAIAVTYP GASAQTVQDT
VVQVIEQQLN GIDNLRYVSS ESNSDGSMTI TATFEQGTNP DTAQVQVQNK LNLATPLLPQ
EVQQQGIRVT KAVKNFLLVI GLVSEDGSMT KDDLANYIVS NMQDPISRTA GVGDFQVFGA
QYAMRIWLDP AKLNKFQLTP VDVKTAVAAQ NVQVSSGQLG GLPALPGTQL NATIIGKTRL
QTAEQFESIL LKVNKDGSQV RLGDVAQVGL GGENYAVSAQ FNGKPASGLA VKLATGANAL
DTAKALRETI KGLEPFFPPG VKAVFPYDTT PVVTESISGV IHTLIEAVVL VFLVMYLFLQ
NFRATIITTM TVPVVLLGTF GILAAAGFSI NTLTMFAMVL AIGLLVDDAI VVVENVERVM
SEEGLPPKEA TKRSMEQIQG ALVGIALVLS AVLLPMAFFG GSTGVIYRQF SITIVSAMGL
SVLVALIFTP ALCATMLKPL KKGEHHTAKG GFFGWFNRNF DRSVNGYERS VGTILRNKVP
FLLAYALIVV GMIWLFARIP TAFLPEEDQG VLFAQVQTPA GSSAERTQVV VDQMREYLLK
DEADTVSSVF TVNGFNFAGR GQSSGMAFIM LKPWDERSKE NSVFALAQRA QQHFFTFRDA
MVFAFAPPAV LELGNATGFD VFLQDRGGVG HAKLMEARNQ FLAKAAQSKI LSAVRPNGLN
DEPQYQLTID DERASALGVT IADINNTLSI ALGASYVNDF IDRGRVKKVY IQGEPSARMS
PEDLQKWYVR NGAGEMVPFS SFAKGEWTYG SPKLSRYNGV EAMEILGAPA PGYSTGEAMA
EVERIAGELP SGIGFSWTGM SYEEKLSGSQ MPALFALSVL FVFLCLAALY ESWSIPIAVV
LVVPLGIIGA LIATSLRGLS NDVYFLVGLL TTIGLAAKNA ILIVEFAKEL HEQGRSLYDA
AIEACRMRLR PIIMTSLAFI LGVVPLTIAS GAGAGSQHAI GTGVIGGMIS ATVLAIFWVP
LFFVAVSSLF GSKEPEKDVT PENPRYEAGQ
