ID   TTGD_PSEPT              Reviewed;         382 AA.
AC   Q9KWV5; E0X9D1; I7B4U8;
DT   19-JUL-2005, integrated into UniProtKB/Swiss-Prot.
DT   05-SEP-2012, sequence version 2.
DT   25-MAY-2022, entry version 86.
DE   RecName: Full=Toluene efflux pump periplasmic linker protein TtgD;
DE   Flags: Precursor;
GN   Name=ttgD; Synonyms=sepA; OrderedLocusNames=T1E_4281;
OS   Pseudomonas putida (strain DOT-T1E).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Pseudomonadales;
OC   Pseudomonadaceae; Pseudomonas.
OX   NCBI_TaxID=1196325;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND INDUCTION.
RC   STRAIN=DOT-T1E;
RX   PubMed=10648517; DOI=10.1128/jb.182.4.937-943.2000;
RA   Mosqueda G., Ramos J.L.;
RT   "A set of genes encoding a second toluene efflux system in Pseudomonas
RT   putida DOT-T1 is linked to the tod genes for toluene metabolism.";
RL   J. Bacteriol. 182:937-943(2000).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND INDUCTION.
RC   STRAIN=DOT-T1E;
RX   PubMed=11251828; DOI=10.1046/j.1365-2958.2001.02310.x;
RA   Duque E., Segura A., Mosqueda G., Ramos J.L.;
RT   "Global and cognate regulators control the expression of the organic
RT   solvent efflux pumps TtgABC and TtgDEF of Pseudomonas putida.";
RL   Mol. Microbiol. 39:1100-1106(2001).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND SEQUENCE REVISION.
RC   STRAIN=DOT-T1E;
RA   Daniels C., Godoy P., Duque E., Molina-Henares M.A., de la Torre J.,
RA   Del Arco J.M., Herrera C., Segura A., Guazzaroni M.E., Ferrer M.,
RA   Ramos J.L.;
RT   "Global regulation of food supply by Pseudomonas putida DOT-T1E.";
RL   Submitted (SEP-2010) to the EMBL/GenBank/DDBJ databases.
RN   [4]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=DOT-T1E;
RX   PubMed=23815283; DOI=10.1111/1751-7915.12061;
RA   Udaondo Z., Molina L., Daniels C., Gomez M.J., Molina-Henares M.A.,
RA   Matilla M.A., Roca A., Fernandez M., Duque E., Segura A., Ramos J.L.;
RT   "Metabolic potential of the organic-solvent tolerant Pseudomonas putida
RT   DOT-T1E deduced from its annotated genome.";
RL   Microb. Biotechnol. 6:598-611(2013).
RN   [5]
RP   FUNCTION, AND EFFLUX PUMP SUBSTRATES.
RC   STRAIN=DOT-T1E;
RX   PubMed=11395460; DOI=10.1128/jb.183.13.3967-3973.2001;
RA   Rojas A., Duque E., Mosqueda G., Golden G., Hurtado A., Ramos J.L.,
RA   Segura A.;
RT   "Three efflux pumps are required to provide efficient tolerance to toluene
RT   in Pseudomonas putida DOT-T1E.";
RL   J. Bacteriol. 183:3967-3973(2001).
CC   -!- FUNCTION: The periplasmic linker protein component of an inducible
CC       organic solvent efflux pump. Involved in export of toluene and styrene
CC       but not of m-xylene, propylbenzene or ethylbenzene. Is not involved in
CC       antibiotic or AMP efflux. {ECO:0000269|PubMed:11395460}.
CC   -!- SUBCELLULAR LOCATION: Cell inner membrane {ECO:0000305}; Lipid-anchor
CC       {ECO:0000255|PROSITE-ProRule:PRU00303}.
CC   -!- INDUCTION: The ttgDEF operon is induced in the presence of toluene and
CC       styrene, but not m-xylene. {ECO:0000269|PubMed:10648517,
CC       ECO:0000269|PubMed:11251828}.
CC   -!- SIMILARITY: Belongs to the membrane fusion protein (MFP) (TC 8.A.1)
CC       family. {ECO:0000305}.
CC   -!- SEQUENCE CAUTION:
CC       Sequence=ADI95410.1; Type=Erroneous initiation; Note=Extended N-terminus.; Evidence={ECO:0000305};
CC       Sequence=AFO50110.1; Type=Erroneous initiation; Note=Truncated N-terminus.; Evidence={ECO:0000305};
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DR   EMBL; GQ884177; ADI95410.1; ALT_INIT; Genomic_DNA.
DR   EMBL; CP003734; AFO50110.1; ALT_INIT; Genomic_DNA.
DR   AlphaFoldDB; Q9KWV5; -.
DR   SMR; Q9KWV5; -.
DR   TCDB; 2.A.6.2.10; the resistance-nodulation-cell division (rnd) superfamily.
DR   EnsemblBacteria; AFO50110; AFO50110; T1E_4281.
DR   KEGG; ppx:T1E_4281; -.
DR   PATRIC; fig|1196325.3.peg.4238; -.
DR   HOGENOM; CLU_018816_2_1_6; -.
DR   Proteomes; UP000006503; Chromosome.
DR   GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0022857; F:transmembrane transporter activity; IEA:InterPro.
DR   InterPro; IPR043602; CusB_dom_1.
DR   InterPro; IPR032317; HlyD_D23.
DR   InterPro; IPR006143; RND_pump_MFP.
DR   Pfam; PF00529; CusB_dom_1; 1.
DR   Pfam; PF16576; HlyD_D23; 1.
DR   TIGRFAMs; TIGR01730; RND_mfp; 1.
DR   PROSITE; PS51257; PROKAR_LIPOPROTEIN; 1.
PE   2: Evidence at transcript level;
KW   Cell inner membrane; Cell membrane; Coiled coil; Lipoprotein; Membrane;
KW   Palmitate; Signal; Transport.
FT   SIGNAL          1..23
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00303"
FT   CHAIN           24..382
FT                   /note="Toluene efflux pump periplasmic linker protein TtgD"
FT                   /id="PRO_0000018719"
FT   COILED          100..136
FT                   /evidence="ECO:0000255"
FT   LIPID           24
FT                   /note="N-palmitoyl cysteine"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00303"
FT   LIPID           24
FT                   /note="S-diacylglycerol cysteine"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00303"
SQ   SEQUENCE   382 AA;  41558 MW;  F0DD9A1979592757 CRC64;
     MRLERALRAR QLIPLAAIWL LVGCGKQETV ESTAVPPEVG VYTVKAQALT LTTDLPGRTS
     AYRVSEVRPQ ASGILQKRMF VEGAEVKQGE QLYQIDPRTY EALLARAEAS LLTAQNLARR
     YERLLDTNAI SQQQYDDAMA TWKQAQAEAQ MARINMQYTK VLAPITGRIG RSAVTEGALV
     TNGQAQELAT VTQLDPIYVD VNQPITRLLG LKRALESGRL QRVGDNQAQV SLTLDDGTPY
     PLKGVLKFSE VSVAPSTGSV TLRAEFPNPD HKLLPGMFVH ALLNEGEQQA AILVPHQAVG
     RDARGVPTVW VVKPDNTVES REVQTLQTVG NAWLLGAGIN DGERVITEGV QLARSGITVK
     PVAAKNVKLM SEFGSQVQAQ AH